mechanisms for activating cell death in cancer cells pdf

... of syncytium formation in these cells Interestingly, RSV BT4a-infected PBECs and mock-infected controls were indistinguishable in terms of CPE Similar data were obtained following infection at ... hpi for cytokine/chemokine measurements Cells were scraped into the remaining 250 μL medium, harvested, sonicated, centrifuged at 250 × g for 15 min, snap frozen and stored in liquid nitrogen for ... passaged in vitro in continuous cell lines, such as HEp2 In contrast, the clinical isolates have not RSV A2 would therefore be expected to be better adapted to replication in HEp-2 cells than the clinical...

Ngày tải lên: 11/08/2014, 21:22

... p27 found in quiescent cells suggest that the p27 gene also plays a role in maintaining cells in G0 (Hengst et al., 1996) As p27 has a nuclear localization signal in its -COOH terminus, loss ... of our hypothesis in determining clinical significance of cancer stem -cell marks in colorectal cancer Our main objective is to understand the clinical value of cancer stem -cell markers and their ... relevance of cancer stem cells Taking into consideration that the working mechanisms of the cancer stem -cell concept may incorporate many molecular pathways (Barozzi et al., 2002), we have examined a...

Ngày tải lên: 03/10/2015, 20:57

... ATP binding site serves as a docking site for specific binding of cytoplasmic signaling proteins containing Src homology-2 (SH2) and protein tyrosine binding (PTB) domains These proteins in turn ... shock protein 90-binding geldanamycin inhibits cell proliferation, down- regulates oncoproteins, and inhibits epidermal growth factor-induced invasion in thyroid cancer cell line J Clin Endocrionol ... tyrosine kinase inhibitors Inhibiting the activity of tyrosine kinases by low molecular weight compounds capable of interfering with either ligand binding (in the case of receptor tyrosine kinases)...

Ngày tải lên: 03/11/2012, 09:57

... conditions coexist in the same lobe, the time required for the diagnosis of cancer is often considerable Outstanding progress in imaging techniques has, though, led to increasing accuracy in the diagnosis ... easily missed in patients with active or inactive TB, which commonly delays the diagnosis of lung cancer due to masking In TB patients with a predominant or growing nodule, coexisting cancer should ... eccentrically in 15 cases and centrally in five Cavitation within the tumor was observed in five patients (9.8%), and mediastinal lymphadenopathy in 42 (82.4%) The stage of non-small -cell lung cancer...

Ngày tải lên: 22/03/2014, 18:20

... H358), two large carcinoma cell lines (H460, H661), and one squamous cell carcinoma cell line (H226), as well as one small cell lung cancer cell line (SCLC) (H841) were obtained from the American ... SQ participated in acquiring clinical samples and follow-up clinical information MSC participated in study design and coordination, data analysis and interpretation and final writing of the manuscript ... exist within the individual gene promoters for certain genes in lung cancer carcinomas MS-MLPA is only based on a single CpG site compared to an average of 4-6 CpG sites in MS-PCR assays Since only...

Ngày tải lên: 18/06/2014, 16:20

... few coordinated “pioneering cancer cells form deep penetrating invasion tunnels along a line, paving the way for follower cancer cells Among these four patterns, washing pattern may correlate ... barrier, forming a potential invasive tunnel (E, F) Linear pattern: cancer cells digest the ECM main along a line (G, H) Type IV collagen was abruptly degraded at a point, several cells were migrating ... of tumor cells and their microenvironment in cancer invasions Both of tumor cells and their microenvironment are involved in cancer invasions Invasion is the first observable step of cancer progression...

Ngày tải lên: 18/06/2014, 16:20

... increases (cells/ uL) -200 200 400 600 months 50 cells/ L 100 200 300 Baseline CD4 counts (cells/ uL) CD4 increases (cells/ uL) -200 200 400 600 100 200 300 Baseline CD4 counts (cells/ uL) 100 cells/ ... predictors of SO-CD4 The independent variables included age, sex, baseline CD4 cell counts, body mass index (BMI), baseline hemoglobin, initial ART regimen, magnitude of CD4 increase in first months and ... of the magnitude of the CD4 cell increase [a CD4 count increase of < 50 CD4 cells/ μl after months of ART [3-6];

Ngày tải lên: 10/08/2014, 05:21

... cisplatin plus irinotecan versus carboplatin plus paclitaxel, cisplatin plus gemcitabine, and cisplatin plus vinorelbine for advanced non-small -cell lung cancer: Four-Arm Cooperative Study in Japan ... Rusch V, Sobin L: International Association for the Study of Lung Cancer International Staging Committee; Participating Institutions The IASLC Lung Cancer Staging Project: proposals for the revision ... chemoradiation clinical trials for locally advanced non-small cell lung cancer in Japan Int J Clin Oncol 2008, 13:112-6 Farray D, Mirkovic N, Albain KS: Multimodality therapy for stage III nonsmall-cell...

Ngày tải lên: 10/08/2014, 09:22

... immunostaining in sections and further graded into four groups: + : < 5% of tumor cells stained; ++ : 5-25% of tumor cells stained; +++ : > 25-50% of tumor cells stained; ++++ : > 50% of tumor cells ... operation to cancer- related death only Cases were included according to the following inclusion criteria: having archived formalin-fixed, paraffin-embedded specimens available; having complete clinicopathological ... competing interests Received: November 2010 Accepted: January 2011 Published: January 2011 References Patel T: Increasing incidence and mortality of primary intrahepatic cholangiocarcinoma in the...

Ngày tải lên: 10/08/2014, 10:20

... positive expression for CD45, negative staining for CD15 and their high expression of CD68 For antibody staining, cells were incubated with the respective antibodies for 40 minutes and analyzed ... RAST was performed for forty inhalation and food allergens (Sanofi Diagnostics Pasteur, Inc, Chaska, MN) All asthma patients used inhaled bronchodilators and nine asthma patients used inhaled corticosteroids ... performed according to the ATS guidelines [14] The clinical indication for BAL in pediatric asthmatic patients was the severity and chronicity of the asthmatic disease that prompted us to perform...

Ngày tải lên: 12/08/2014, 11:21

... regressing BMLs Logistic regression analysis was used to examine the association between baseline BMLs (absent versus present) and increases in BMLs (no increase or incident BML versus increase or incident ... used to account for the correlated readings within an individual and examine the association between changes in WOMAC scores (pain, function, and stiffness) and continuous changes in BML size Standard ... demonstrating that the association between changes in function and changes in BML size is mediated by changes in pain In those without ROA, a one SD increase in total BML size led to a 1.13-unit increase...

Ngày tải lên: 12/08/2014, 15:22

... particularly useful in diagnosing incident cancers in otherwise normal tissues Indeed, this approach discriminates normal and neoplastic tissues in various cancer types, including CLL, breast cancer, glioblastoma, ... of essential proteins responsible for accelerating the cell cycle, including cyclin D and retinoblastoma [56,57] Moreover, alterations in the machinery responsible for processing miRNA contributes ... expression in lung cancer cells in vitro to inhibit colony formation [36] Key downstream targets for let-7 include the human Ras family of proteins, oncogenes that are commonly mutated in many human...

Ngày tải lên: 16/03/2014, 01:20

... shifting in such a way that the continued increase in body fatness is increasingly being recognized as underlying several chronic disease states This phenomenon is slowing or even reversing gains ... of insulin receptor genes have also been generated, in order to assess insulin action in individual tissues These include the muscle-specific insulin receptor knockout mice, the liver insulin ... (occasionally called insulin resistance syndrome) refers to a constellation of clinical findings including obesity, hypertension, hyperlipidemia, and insulin resistance, with increased risk for type diabetes...

Ngày tải lên: 22/03/2014, 19:20

... a single gene or a few genes in cancer cells The studies have uncovered the roles of individual genes in the uncontrolled behavior of cancer cells Studying the functional roles of genes in cancer ... highly invasive cell lines) They identified 80 loci of high level of amplification in 35 different cell lines These include increased expression of known oncogenes involved in breast cancer, for ... transformation in vitro (40) AURKA interacts with TPX2 and plays a role in centrosome maturation and spindle formation (41) The polo-like kinase (PLK1) is important in spindle formation and cell...

Ngày tải lên: 30/03/2014, 09:20

... ubiquitin -activating enzymes (E1), ubiquitin-conjugating enzymes (E2 or UBC), and ubiquitin–protein ligases (E3) Multiubiquitin chains are formed on lysine side chains on the target protein, which ... resulting in cells that are already in G2 at the time of cell separation (14) The G2/M transition is the major control point in the cell cycle of fission yeast and determines the timing of entry into ... (a G2 cyclin) (reviewed in ref 20) Cyclins bind to Cdc28/Cdc2, forming an active complex, which is associated with histone H1 kinase activity In order to bind, cyclins recognize a binding motif...

Ngày tải lên: 08/04/2014, 12:50

... of these cells to collagencoated surfaces and induces cell spreading [16] RAGE binds laminin and Collagen I and IV in vitro, but not fibronectin Thus RAGE plays a role in anchoring AT I cells to ... proteins (formerly HMG14 and HMG17) have nucleosomal binding domains HMGB proteins (formerly HMG1 through HMG4) are distinguished by having two DNA-binding boxes that have a high affinity for ... monomer of protein However upon addition of Zn++, higher aggregates form [112] S100G was also known as Vitamin D-dependent calcium-binding protein, intestinal CABP, Calbindin-3, and Calbindin-D9k [113]...

Ngày tải lên: 18/06/2014, 15:20

Ngày tải lên: 18/06/2014, 19:20

... The European Organization for Research and Treatment of Cancer QLQ-C30: a quality-of-life instrument for use in international clinical trials in oncology J Natl Cancer Inst 1993, 85(5):365-376 ... Group Am J Clin Oncol 1982, 5(6):649-655 Wyrwich KW, Nienaber NA, Tierney WM, Wolinsky FD: Linking clinical relevance and statistical significance in evaluating intra-individual changes in health-related ... 2007, 5:70 clinical trials in oncology are increasingly incorporating generic preference-based measures such as EQ-5D EQ-5D is an indirect measure of utility for health that generates an index-based...

Ngày tải lên: 18/06/2014, 22:20

... been aforementioned for A baumannii isolates, may promote colistin resistance in MDR K pneumoniae strains Colistin’s pharmacokinetic properties The main pharmacokinetics (PK) of colistin are ... PK/PD relationship of colistin against P aeruginosa has been examined recently in a vitro model A significant finding of the study was that colistin efficacy against P aeruginosa was best correlated ... CMS doses in humans, especially in the ICU setting Optimizing colistin dose based on PK/PD properties The optimal dose of colistin has not been determined in the ICU setting, because since there...

Ngày tải lên: 21/06/2014, 01:20