mechanism of fatigue in the absence of defects and inclusions
Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
... catalyzed the reduction of the 9,11-endoperoxide group of PGH2 to produce PGF2a in the presence of NADPH, which was defined as the PGFS activity, and the isomerization of PGH2 to PGD2 in the absence of ... 7.0 by the wild-type and mutants (D) Typical fluorescence quenching curves of wild-type AKR1B1 and its mutants The binding of NADP+ to these proteins was determined by measuring the decrease in fluorescence ... 1% in the Y48F, K77L and H110F mutants, to 4% in the D43N mutant and to 12% in the H110A mutant The AKR activity of the D43N and K77L mutants was partly restored in the charge-recovered (D43E and...
- 11
- 390
- 0
Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... shown in Fig 1B, the distance between the two termini of the inserted Ca2+-binding sites in the model structure of the EF-hand of STIM1 is within ˚ 15 A Accordingly, a total of six glycine linkers ... Ca2+-coordinating loop positions and was introduced to perturb the Ca2+-binding ability of the grafted EF-hand of STIM1 (B) Modelled structure of the engineered protein with the grafted EF-hand Ca2+-binding ... it retains its native structure after the insertion of foreign sequences and in the presence and absence of Ca2+ ions, so that the in uence from the host protein to the inserted sites is minimized...
- 9
- 465
- 0
Báo cáo Y học: Inhibition of the SERCA Ca21 pumps by curcumin Curcumin putatively stabilizes the interaction between the nucleotide-binding and phosphorylation domains in the absence of ATP pot
... ATP into contact in the absence of ATP, curcumin is then able to bind to the ATPase (possibly at the hinge region) locking the two domains together and therefore precluding ATP binding (i.e inhibiting ... concentrations of curcumin (25 mM ) the Km for the catalytic site is also possibly increased In order to further assess the possibility of curcumin affecting the interaction of ATP binding to the ATPase, ... The rate of quenching was then monitored (Fig 2) After addition of curcumin, the rate of quenching was increased, showing that curcumin permeabilizes the membrane to metal ions The increase in...
- 10
- 594
- 0
Báo cáo khoa học: A steady-state competition model describes the modulating effects of thrombomodulin on thrombin inhibition by plasminogen activator inhibitor-1 in the absence and presence of vitronectin ppt
... PAI-1/VN complexes, suggests the involvement of the chondroitin sulphate of rl-TM in the binding of PAI-1/VN Indeed, both in the absence and presence of PAI-1, the interaction of rl-TM with immobilized ... binding of TM by thrombin Binding of the carboxy-terminal part of the reactive center loop of PAI-1 in the small cleft formed by the 60-loop and VR1 loop can be envisioned The kinetics of the interaction ... Pro37 and Gln38 are involved in the binding of TM to thrombin [27,31] These residues therefore comprise the most likely overlapping binding site for TM and PAI-1 on thrombin, as they are also of...
- 10
- 483
- 0
Báo cáo khóa học: Protein assembly of photosystem II and accumulation of subcomplexes in the absence of low molecular mass subunits PsbL and PsbJ pdf
... always hold true In rapidly expanding young leaves in particular, some of the PSII core proteins and all of the OEC proteins can accumulate in thylakoids in the absence of any assembly of PSII, as ... and PsbJ is critical in providing proper docking sites, either directly or indirectly, by modifying the conformation of PSII on the lumenal side, making efficient binding of PsbQ and PsbP of the ... accumulating all three proteins of the OEC and traces of the D2 and CP43 core proteins as well (Fig 1B) The presence of minor amounts of D2 in both DpsbE and DpsbF supports the suggestion that the...
- 12
- 401
- 0
Báo cáo sinh học: " Clearance of low levels of HCV viremia in the absence of a strong adaptive immune response" pptx
... show the sum of SI values of the proliferation assay and the sum of specific interferon-gamma spots (spots in the presence of antigen – spots in the medium control) against individual HCV proteins ... detected against at least one HCV protein either in the interferon-gamma ELISPOT assay or in the proliferation assay in five of the seven patients (figures and 2) None of the patients who remained negative ... remained negative throughout follow-up in of the seven cases (Table 1) We also investigated reactivity against single HCV proteins by the INNO-LIA HCV III assay A faint band against the C2 protein...
- 11
- 528
- 0
Báo cáo hóa học: " Clearance of low levels of HCV viremia in the absence of a strong adaptive immune response" potx
... show the sum of SI values of the proliferation assay and the sum of specific interferon-gamma spots (spots in the presence of antigen – spots in the medium control) against individual HCV proteins ... detected against at least one HCV protein either in the interferon-gamma ELISPOT assay or in the proliferation assay in five of the seven patients (figures and 2) None of the patients who remained negative ... remained negative throughout follow-up in of the seven cases (Table 1) We also investigated reactivity against single HCV proteins by the INNO-LIA HCV III assay A faint band against the C2 protein...
- 11
- 440
- 0
Báo cáo y học: "Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells" ppsx
... generated, a combination of an IgG2b antibody of the clone M2139 binding to the J1 epitope (amino acids 551–564) and an IgG2a antibody of the clone CIIC1 binding to the C1I epitope (amino acids 358–363) ... important roles either during the initiation phase of arthritis or in the perpetuation and exacerbation of the disease after the onset, or they might simply maintain immunity to CII and perpetuate ... cells again did not affect the initiation phase of the disease; instead, the effect was noted in the more chronic phase of the disease Still, co-transfer of T cells into MMC mice resulted in an...
- 7
- 434
- 1
Báo cáo khoa học: "Pancreatic insulinoma co-existing with gastric GIST in the absence of neurofibromatosis-1" pot
... an insulinoma in the absence of NF-1 The GIST tumour was only incidentally discovered by the operating surgeon during enucleation of the insulinoma in our case The literature contains only published ... like the initial one in our case, are usually an incidental surgical finding, while large tumours are usually symptomatic In our case, following resection of the insulinoma and the small incidental ... procedures on the patient and participated in designing the report PT analyzed and reported on all the histological specimens in the case and participated in designing the report All authors read and approved...
- 5
- 457
- 0
Báo cáo y học: " Effector mechanisms of interleukin-17 in collagen-induced arthritis in the absence of interferon-γ and counteraction by interferon-γ" pot
... by injecting 20 μg of CII in the left ears and vehicle in the right ears Bars in Figure 3d represent the percentages of swelling in the CII-challenged ears, normalized to the swelling of the ... importance of STAT1 and IRF-1 in the inhibitory action of IFN-γ, the percentage of inhibition of the expression of GCP-2, RANKL and IL-6 mRNA induced by synergistic action of IL-17 and TNF-α was ... ear and with 20 μl PBS in the right ear DTH response was calculated as the percentage swelling (the difference between the increase of thickness of the left ear and the right ear, divided by the...
- 13
- 560
- 0
Báo cáo y học: "Candida albicans genome sequence: a platform for genomics in the absence of genetics" potx
... sequencing has profoundly altered our understanding of this organism Early assemblies of the C albicans genome sequence revealed a mating-type (MAT-like) locus [9] that led to the engineering of ... Candidosis 2nd edn London: Bailliere Tindall; 1988 Calderone RA: Candida and Candidiasis Washington, DC: ASM Press; 2002 Fonzi W, Irwin M: Isogenic strain construction and gene mapping in Candida ... sequencing may also contribute to the fitness of C albicans in at least one of the niches it occupies and/ or to its pathogenicity C albicans also contains multiple copies of genes involved in the...
- 3
- 400
- 0
Báo cáo y học: "Aspergillus antigen induces robust Th2 cytokine production, inflammation, airway hyperreactivity and fibrosis in the absence of MCP-1 or CCR2" pptx
... for MCP-1 and CCR2 in other models of inflammation and fibrosis, including different allergic airway disease models [25,27,33] Those findings indicate that the role of MCP1 and CCR2 in allergic ... spaces and scattered infiltrates in the lung parenchyma (Fig 2A and 2B) The inflammatory infiltrates consisted of numerous eosinophils as well as other cell types To determine the airway inflammatory ... significantly increased in mice treated with MCP-1 neutralizing antibody and in CCR2-deficient mice However, these increases in fibrosis were seen in the setting of impaired clearance of conidia and a...
- 12
- 290
- 0
Bóa cáo y học: "The incidence and outcome of septic shock patients in the absence of early-goal directed therapy" pdf
... EGDT) was still only 1.6% and its non-NFR mortality lower These findings are relevant to other institutions considering the application of EGDT and to the powering of future randomized controlled ... ventilation in the ICU Of these 52 study patients, 37 (71.2%) survived hospital admission, two (3.8%) died in the ED, eight (15.3%) died in the ICU and six (11.5%) died in the ward Using the lactate ... summarizes their primary sources of infection Available online http://ccforum.com/content/10/3/R80 Table Table Clinical and laboratory findings in the emergency room Primary source of infection in patients...
- 7
- 248
- 0
Báo cáo y học: "Mechanical ventilation using non-injurious ventilation settings causes lung injury in the absence of pre-existing lung injury in healthy mice" pdf
... behind VILI and the contribution of MV to lung injury in the absence of pre-existing lung injury Competing interests The authors declare that they have no competing interests Page of 11 (page ... cytokines were measured in the BALF, while in our study and in the study of Vaneker and colleagues cytokines were measured in lung homogenate Maybe cytokines were still in the sub-epithelium and ... 7.5 l/g of induction KMA mix (consisting of 1.26 ml ketamine, 0.2 ml medetomidine and ml atropine) To maintain anaesthesia, 10 l/g of maintenance KMA mix (consisting of 0.72 ml ketamine, 0.08...
- 11
- 463
- 0
Securities Trading in the Absence of Dealers:Trades, and Quotes on the Tokyo Stock Exchange
... trading day The mean squared return and spread tend to be elevated at the beginning and end of the trading day The volume tends to be elevated at the beginning and end of the trading sessions.5 The ... One hypothesis involves the size of the order It was noted in the discussion of the institutional features of the TSE that the display terminal at a member’s lead, office shows the size of the orders ... article investigates the properties of intraday trades and quotes on the TSE In comparison with most of the worlds other principal equity markets, the TSE is distinctive in the absence of dealers In...
- 30
- 303
- 0
Mechanism of action and binding mode revealed by the crystal structures of key enzymes in plants sugar metalbolic pathway
... 0.01-0.02 mM of the SPS in the sample cell and 0.1-0.2mM of F6P in the injector (Figure 2.9) Injection volumes of 4-5 µL each were used and the number of injections 24 was 60 The heat of dilution ... the binding residues of H orenii SPS Superimposition of one docked-UDP ligand and the actual UDP ligand Superimposition of one docked-ADP ligand and the actual ADP ligand Superimposition of the ... identified in the course of a random sequence analysis of the H orenii genome (Mijts and Patel, 2001) The following chapters of this thesis report the structures and catalytic mechanisms of SPS and...
- 136
- 309
- 0
Cambridge.University.Press.The.Crisis.of.Literature.in.the.1790s.Print.Culture.and.the.Public.Sphere.Nov.1999.pdf
... Echoes of their mix of promise and threat, anticipation and dread, resound in the writings of the eighteenth and early nineteenth centuries in Britain – a time and a place when the newly disturbing ... these values, could be contained The emotional intensity of the backlash against the intrusions of these subaltern groups suggests the importance of reading these developments in ways which interfuse ... every rank, of every age, and of both sexes They are intimately acquainted with every language, the dead as well as the living To the cultivation of letters they join that of the arts; and the mechanics...
- 316
- 972
- 2
Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
... was further investigated by determining the in uence on the glycone binding of the Sfbgly interactions with the aglycone Thus, the effect of the alteration of the glycone structure in the stability ... and M453 are part of the aglycone-binding site of Sfbgly and interact with the initial pentyl moiety formed by methylene groups to of the alkyl-type aglycones On the other hand, the binding of ... E190 and E194 are positioned in an internal region of the aglycone-binding site, probably subsite +1, because they in uence the binding of the first glucose unit of the aglycone On the other hand,...
- 12
- 731
- 0
Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
... modification may in uence many of the physicochemical and biological properties of the proteins, such as protein folding, stability, targeting, dynamics and ligand binding, as well as cell-matrix and cell–cell ... N-linked oligosaccharide side-chains are important for the GABA transport activity 1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role of the ... misfolding of this protein, resulting in its partial retention in the ER and its rapid digestion thereafter [16,19] Accordingly, our results indicate that the intracellular trafficking of N-glycosylation...
- 14
- 654
- 0
Tài liệu Báo cáo khoa học: Unraveling the catalytic mechanism of lactoperoxidase and myeloperoxidase A reflection on some controversial features Elena Ghibaudi and Enzo Laurenti docx
... oxidize them [19] On the other hand, this fact does not represent proof of the presence of one or two oxidizing equivalents on the decay product of Cpd I The EPR spectrum of a spin-trapped radical in ... pocket) must be crucial in playing on kinetic equilibria of these enzymes and determining whether the peroxidase or catalase function has to prevail The role of water ÔsittingÕ in the active site has ... LPO and MPO They deal with the the major importance of catalase activity in MPO with respect to LPO, the different sensitivity of the enzymes to Cpd III inactivation and the redox potential of the...
- 10
- 529
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25