measurements data processing and analysis

... sensitivity and information content in NMR spectra Molecular biology and medicine were revolutionized by the advent of multidimensional NMR spectroscopy and NMR imaging Apart from chemical analysis and ... more demanding than chemical analysis and medical imaging as they are rather diverse All three major methodical branches of NMR are needed They are relaxometry, imaging, and spectroscopy And imaging ... multidimensional data the procedure (2.40) has to be applied sequentially for all dimensions In the expressions Qn and MCk the naturals n and k signify the index of the result and measured data vectors,...

Ngày tải lên: 29/05/2014, 18:32

... to the conception and design of the study and planning of the work that led to the manuscript, the acquisition, and interpretation of data, and has been involved in drafting and critically revising ... to the design of the study and planning of the work that led to the manuscript, the acquisition, analysis and interpretation of data, and has been involved in drafting and critically revising the ... substantial contributions to the conception and design of the study, the acquisition, analysis and interpretation of data, and has been involved in drafting and critically revising the manuscript...

Ngày tải lên: 11/08/2014, 05:21

... to the conception and design of the study and planning of the work that led to the manuscript, the acquisition, and interpretation of data, and has been involved in drafting and critically revising ... to the design of the study and planning of the work that led to the manuscript, the acquisition, analysis and interpretation of data, and has been involved in drafting and critically revising the ... substantial contributions to the conception and design of the study, the acquisition, analysis and interpretation of data, and has been involved in drafting and critically revising the manuscript...

Ngày tải lên: 11/08/2014, 16:21

... Indeed, several long-range NOEs were observed between T47 and Q61, between H72 and V78, and between H72 and L81 (Table 1) The NOESY data were scrutinized extensively to identify additional longrange ... unaffected (Figs and 3) and their backbone mobility remains high in the presence of membrane-mimicking micelles (Fig 8B) PRE data suggest buffer-exposed side chains of S53 and S57 (Figs 7, and 10) that ... tight loop and the C-terminus of VpUcyt In contrast, most cross-peaks originating from helices and 3, as well as from residues between helix and the tight loop, were least affected and remained...

Ngày tải lên: 18/02/2014, 06:20

... post-translational modifications ) protein processing [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular ... et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ) ... Following MS ⁄ MS analysis (Fig 2D), the latter peptide is identified as TEAPLNPKANR(106-116) and constitutes a neo-N-terminus indicative of HIV-1mediated protein processing Processing of b-actin...

Ngày tải lên: 18/02/2014, 16:20

... rigidity) and contour length, enabling the validation of the continuum model of biomolecules such as microtubule and DNA In last decade, since the pioneering works by Bustamante and coworkers13,14 and ... nanowires,7-9 and biological fibers such as microtubules.10 Fluorescence method for a cantilevered fibers such as microtubules11 and/ or DNA molecules12 has allowed one to understand the relationship ... materials and their mechanical properties such as Young’s modulus, quantitatively and/ or qualitatively comparable to mechanical properties of biological protein materials obtained from experiments and/ or...

Ngày tải lên: 22/02/2014, 08:20

... by tandem mass spectrometry A protein complex is separated by 1D PAGE and bands of interest are cut from the gel, digested with trypsin and the generated peptides are partially sequenced by tandem ... from the tandem mass spectrum Mass and partial amino-acid sequence data are simultaneously searched against protein or nucleotide sequence databases for protein identification 576 A Bauer and B Kuster ... pulldown’ The standard precipitation experiment with the aid of a recombinant GST fusion protein has been widely used for the discovery and analysis of individual protein interactions and, to a lesser...

Ngày tải lên: 31/03/2014, 07:20

... assays and measurements, performed the statistical analysis and drafted the manuscript AF conceived the study, designed and conducted the study and drafted the manuscript AS collected the samples and ... discarded, and the cells remaining in the tube were collected Magnetic enrichment was repeated twice Cell suspension was finally split in two fractions and stained with CD45 (BD Biosciences) and CK and ... cytometry or immunocytochemical analysis for visualization and quantification Immunomagnetic separation was successful on clinical samples, and superior to the standard Ficoll density centrifugation...

Ngày tải lên: 18/06/2014, 19:20

... assays and measurements, performed the statistical analysis and drafted the manuscript AF conceived the study, designed and conducted the study and drafted the manuscript AS collected the samples and ... discarded, and the cells remaining in the tube were collected Magnetic enrichment was repeated twice Cell suspension was finally split in two fractions and stained with CD45 (BD Biosciences) and CK and ... cytometry or immunocytochemical analysis for visualization and quantification Immunomagnetic separation was successful on clinical samples, and superior to the standard Ficoll density centrifugation...

Ngày tải lên: 20/06/2014, 03:20

... experiments and data analysis in the laboratory Both authors designed and coordinated experiments EPCL provided important advice and financial support MP wrote manuscript Both authors read and approved ... Preparation of buffer solutions and stock solutions All buffer solutions were prepared and pH-adjusted using sodium hydroxide (10 M, M, and M) and hydrochloric acid (1 M and 0.5 M) All CE separation ... approximately 20 mM and the mixture was left to stand for 10 minutes The activated QDs were purified of unreacted reagents and byproducts by dialysis using 100 kDa MWCO microcentrifuge tubes and re-suspended...

Ngày tải lên: 11/08/2014, 00:22

... shot and multi-slice CT scans were performed for each block (GE Medical Systems, Lightspeed, USA) at 120 kV and 200 mA Analysis The data obtained were stored in a digital format (DICOM) and transferred ... Sources of support and financial interest: none Authors’ contributions CS, MR, MS, CW, MRu, and NCG conceived of the work and participated in its design and coordination CS and MR made substantial ... design and coordination CS and MR made substantial contributions to data acquisation and conception of manuscript CS and MR drafted and designed the manuscript CW, MRu, NCG have been involved in drafting...

Ngày tải lên: 11/08/2014, 20:21

... contributions PEC, JS, BGH, HE, UK, and WHR designed the studies, performed the studies, analyzed the data, and interpreted the data JTE and JDR provided the psoriatic arthritis and ankylosing spondylitis ... 1:2 dilution and then 20 μl of digoxigenylated monoclonal antibody Standards specific to each type of chip were included in the assays using the CID 1, CID 3, CID 4, and bone chips, and levels ... calculated on the basis of the standard curves generated Results for the synovial antigen and chips (for which standards have not yet been generated) were reported and analyzed as signal intensities...

Ngày tải lên: 12/08/2014, 17:22

... To my dear uncle and my aunt Khem Kandel and Laxmi Kandel: your support and friendship has been such a help during this time Lastly, to my sweetheart Kriti Acharya, your love and support provided ... chemotherapeutic agent and in the mobilization and conditioning regimens for blood and marrow transplantation Reviewing the chemistry and pharmacology of cyclophosphamide is crucial for understanding its ... mustard and its mechanism of DNA cross linking Figure represents how phosphoramide mustard forms intrastrand and interstrand cross–links between guanine bases from DNA 23 B Hypothesis and approaches...

Ngày tải lên: 24/08/2014, 09:51

... routing out and filling with a proper sealant members If they leak, random cracks should be routed and sealed with flexible sealants (Fig 3.6 and 3.7) 3.2.2 Seals for isolation joints and expansion ... entrance and exit lanes, turn areas, and ramps can be expected to require more maintenance than parking stalls and flat drive lanes and should be monitored accordingly 3.3.2 Beams, columns, and walls—Beam ... joints and deck-slab cracks Vertical surfaces of columns and bumper walls are also susceptible to damage by ponded water and salt water splashed from moving vehicles Beams and columns adjacent to and...

Ngày tải lên: 24/10/2014, 21:59

Ngày tải lên: 14/11/2014, 06:20

... hysteresis loop Forward and reverse coercive voltages, V+ and V−, nucleation voltages Vc+ and Vc− , + − and forward and reverse saturation and remnant responses, R0 , R0 , Rs+ and Rs− , are shown ... Yeap, A Kumar, L Chen and Haiyan Jiang, "Chapter 3: Fracture toughness and interfacial adhesion strength of thin films: indentation and scratch experiments and analysis" , in CRC Handbook of Nano-Structured ... amplitude (dzz) and Surface potential results for all the samples under stress and magnetic field at location and Table 6.1 Mean domain angle under the unbiased and biased area for undoped and 2%Mg...

Ngày tải lên: 10/09/2015, 08:41

... Characterization of Nogo-B and Systematic Dissection of the Am-Nogo-A 92 3.3.1 Bioinformatics analysis 93 3.3.2 CD and NMR characterization of N- and C-termini of Nogo-B 97 3.3.3 CD and NMR characterization ... use of bioinformatics analysis, and biochemical and biophysical approaches Firstly, two identified inhibitory domains (Ngogo-A_567-748 and Nogo-66) had been studies CD and NMR characterization ... organization of NgBR and its expression and purification 137 Figure 3.6.2 Bioinformatics analysis of NgBR 139 Figure 3.6.3 CD and NMR characterization of the NgBR ectodomain 140 Figure 3.6.4 CD and NMR characterization...

Ngày tải lên: 14/09/2015, 12:53

... rain, fungus, airborne contaminants and aging Also meets standards for environmental cycling for impulse and AC surges • Meets GR-63 standards for transport and storage • NEBS Level compliant ... GR-78-CORE and GR-63-CORE standards • Meets UL 497 standard for paired conductor communications circuit protection • Unique, high-density single-pair design allows pairs to be isolated and easily ... and easily replaced • Meets all standards for controlled environments including temperature, humidity, office vibration and airborne contaminants • Meets all standards for uncontrolled environments...

Ngày tải lên: 10/12/2013, 18:15

... rules and the spectra of hydrogen atoms and hydrogen-like ions 8.5 The emission and absorption processes 8.6 Light Amplification by Stimulated Emission of Radiation (LASER) and the Einstein A- and ... there is no ambiguity and no doubt on the applications level The rules are clear, precise, and all-encompassing, and the predictions and quantitative results are always correct and accurate without ... spectra and atomic structure Condon and Shortley (1963); Herzberg (1944) On molecules and molecular-orbital theory Ballhausen and Gray (1964); Coulson (1961); Gray (1973); Pauling (1967) On lasers and...

Ngày tải lên: 17/03/2014, 14:41