measurement of modulus of rupture 3 point bending test

... 41 3. 3 Selection of polymeric precursors 43 3 .3. 1 Polyimides and its derivatives 43 3 .3. 2 Phenolic resin 45 3. 3 .3 Polyfurfuryl alcohol 46 3. 3.4 ... 297 12 .3. 3.5 Effect of soak time 30 0 12 .3. 4 Surface analysis of NMP derived carbon membrane 30 3 12.4 Conclusions 30 8 x|Page Table of Contents Chapter 13 : Conclusions ... 287 12 .3. 3.2 Critical role of CTAB in tightening chain interaction to achieve molecular sieving membrane 290 12 .3. 3 .3 Effect of pyrolysis temperature 292 12 .3. 3.4 Effect of pyrolysis...

Ngày tải lên: 09/09/2015, 17:54

... an employee fails to carry out any of the above (1 - 3) they can be liable for a fine of up to $10 000 If an employee fails to carry out any of the above (1 - 3) and this causes the death or serious ... be in breach of the law and liable for a fine of up to $200 000 If an employer fails to notify the commissioner of a workplace injury as stated in section they will be in breach of the law and ... the commissioner of any workplace injury that resulted in time off or the death of an employee If an employer hires a contractor to carry out work then the contractor and any of their employees...

Ngày tải lên: 21/08/2012, 17:56

Ngày tải lên: 17/09/2012, 09:13

... Laine, A., and Aubert, J.-P (1999) Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin Biochem J 33 8, 32 5 33 3 Desseyn, J.-L., Guyonet-Dupérat, V., Porchet, N., ... centrifuge ( 23, 000g av) 14 Pour off the supernatant corresponding to the reduced/alkylated “insoluble” mucin complex 3. 3 Isopycnic Density Gradient Centrifugation in CsCl/Guanidinium Chloride 3. 3.1 Isopycnic ... completely (3 11) although large stretches of MUC5AC (12–15) as well as the C-terminal sequences of MUC3 (16) and MUC6 (17) are now known A characteristic feature of mucins is the presence of one...

Ngày tải lên: 23/09/2012, 19:25

... Pathol 33 , 4 53 13 Spicer, S S (1965) Diamine methods for differentiating mucosubstances histochemically J Histochem Cytochem 13, 211– 234 14 Williams, G T (1985) Transitional mucosa of the large intestine ... Pathologica 87, 30 0 30 9 34 Barger, J D and DeLamater, E D (1948) The use of thionyl chloride in the preparation of Schiff's reagent Science 108, 121–122 35 Spicer, S S (1960) A correlative study of the ... 50 g of aluminium potassium sulfate (AlK[SO4]2·12H2O) and dissolve using heat Then add 0.2 g of sodium iodate (NaIO3·H2O) followed by g of citric acid and then 50 g of chloral hydrate (CCl3·CH[OH]2)...

Ngày tải lên: 23/09/2012, 19:25

... the range of 1/500 to 1/20,000) is often provided with commercial conjugates Incubation details are as for primary antibody Wash as in Subheading 3. 2 .3. 3 3. 2 .3. 5 DETECTION Detection of bound enzyme ... curves fitted as in Subheading 3. 1.1.7 3. 2 Dot-Slot and Western Blotting 3. 2.1 Preparation of Dot-Slot Blots for Detection of Mucins (see Note 8) 3. 2.1.1 APPLICATION OF SAMPLES Samples can be either ... Subheading 3. 1.1.1., step and detect as in Subheadings 3. 1.1.5 and 3. 1.1.6 Detection and Quantitation of Mucins 49 3. 1.2.2 ASSAY Select a dilution of antibody and antigen that gives an absorbance of...

Ngày tải lên: 23/09/2012, 19:25

... Digest Dis Sci 23, 35 9 36 4 McQueen, S., Allen, A., and Garner, A (1984) Measurement of gastric and duodenal mucus gel thickness, in Mechanisms of Mucosal Protection in the Upper Gastrointestinal Tract ... (16); (2) the dimensions of the surface pH gradient measured using microelectrodes (17,18); and (3) observation of transverse sections of unfixed mucosa (3) The first two of these methods also measure ... final postfixing of sections at 37 °C Gelatin for water-soluble mounting prepared by dissolving 10 g of gelatin in 60 mL of distilled water mixed with 250 mg of phenol in 70 mL of glycerol Poly-L-lysine-coated...

Ngày tải lên: 23/09/2012, 19:25

... presence of mRNA is only indirect proof of the biosynthesis of the encoded mucin This point was proven by to our knowledge, the only study that actually correlated the levels of mRNA, synthesis of ... the signal of the mRNA of a “housekeeping” protein, i.e., β-actin or glyceraldehydephosphate dehydrogenase, as a measure of sample size (no dimension) 3. 3 Calculation of Biosynthesis of Mucin and ... dimensions of the various calculated values have limited significance, largely because of the use of arbitrary units for each of the measurements of radioactivity, owing to the use of the PhosphorImager...

Ngày tải lên: 23/09/2012, 19:25

... Separation and Identification of Mucin 83 3.4 Identification of Mucin The easiest route to identification of mucin is by the use of MUC-specific antisera However, because of sequence similarities ... dialysis 3. 3 Separation of Mucin Separation of reduced mucin subunits is achieved by using anion-exchange chromatography on a Mono Q HR 5/5 column (Fig 2A) Assessment of the effectiveness of the ... mucins: identification of core proteins and glycoforms Biochem J 31 6, 967–975 Thornton, D J., Howard, M., Khan N., and Sheehan, J K (1997) Identification of two glycoforms of the MUC5B mucin in...

Ngày tải lên: 23/09/2012, 19:26

... electron microscopy of nucleic acid molecules Meth Enzymol X11B, 36 1 37 7 11 Lang D and Mitani, M (1970) Simplified quantitative electron microscopy of biopolymers Biopolymers 9, 37 3 37 9 96 Sheehan ... mucins: identification of core proteins and glycoforms Biochem J 31 6, 967–975 Thornton, D J., Howard, M., Khan, N., and Sheehan, J K (1997) Identification of two glycoforms of the MUC5B mucin in ... Subheading 3. 3.1.2., step 1) on the surface of one-half of a freshly cleaved piece of mica Rejoin the surfaces and leave together for a few minutes Place the mica sandwich in a beaker of 0.2 M...

Ngày tải lên: 23/09/2012, 19:26

... 209,927 ,39 2 1 43, 969,008 2 13, 371,584 2 03, 866,048 2 13, 5 53, 008 216,618,864 91, 930 ,856 200,286 ,32 0 2 03, 002,480 144,096,656 202 ,35 7,184 201,9 93, 024 206,174,464 292,284 ,35 2 404,279,488 532 ,1 83, 264 2 43, 187,696 ... 607,125 ,31 2 179,105,712 271,715,648 149,426,064 7, 537 ,291 84,959,272 169,595,904 29,927 ,38 0 132 , 131 ,184 270,782,624 104,2 13, 960 109, 133 ,576 pmolb 39 1 617 2 43 507 35 711 220 31 8 172 20 106 209 52 1 63 ... Separation of 16 Fmoc–Derivatized Amino Acids Within 35 Time (min) % Mobile phase A (30 mM ammonium phosphate, pH 6.5) % Mobile phase B (15% [v/v] methanol/water) 32 32 .05 34 34 .05 35 17 17 10.8...

Ngày tải lên: 23/09/2012, 19:26

... times) and dry 3. 1 .3 Adsorption of Glycopeptides onto Hyperbond or PVDF Place a piece of Hyperbond or PVDF (a round membrane is preferable, diameter ~0.5 cm; see Note 11) in a lid of an Eppendorf ... Incubate the sample at 4°C for 30 (see Note 16) Wash the membrane alternately in mL of methanol and mL of Milli Q water three times and dry 3. 2 Edman Sequencing 3. 2.1 Glycopeptides Electroblotted ... analysis of glycosylation sites, as little as 10 pmol of material is required, whereas for quantification of the macro- and microheterogeneity, more material is required, depending on the length of...

Ngày tải lên: 23/09/2012, 19:26

... saline (PBS), 0.01 M, pH 7.2 (1 .34 g of Na2HPO4·2H2O, 0 .39 g of NaH2PO4·2H2O, and 8.5 g of NaCl made up to L with distilled water) is used as the buffer base for most of the following buffer reagents ... a minimum of 30 Prepare HOBt and DIC solutions by dissolving in the appropriate amount of DMF (see Note 13) The addition of bromophenol blue to the HOBt to give a final concentration of 0.05 mM ... (see Subheading 3. 4., Notes 13 15, and Fig 3) 1.4 General Comments The techniques described for the analysis of antimucin antibodies using synthetic peptides can provide a great deal of information...

Ngày tải lên: 23/09/2012, 19:26

... (Fig 3) as described in Subheading 3. 5 (see Note 6) 3. 6 .3 Benzyl-α-GalNAc Repeat Subheadings 3. 1. 3. 5 with the addition of benzyl-α-GalNAc at a final concentration of mM to each test dish of COS ... µL of H2O, µL of incubation buffer, and at least U of SacI to a microfuge tube Incubate for h in a 37 ˚ C water bath Add at least U of XbaI to the reaction and incubate for a further hour at 37 ˚C ... organization of the 3' region of the human mucin gene MUC5B J Biol Chem 272, 16,8 73 16,8 83 17 Keates, A C., Nunes, D P., Afdhal, N H., Troxler, R F., and Offner, G D (1997) Molecular cloning of a major...

Ngày tải lên: 23/09/2012, 19:26

... flow rate of 1.5 mL/min Detect by fluorescence with an excitation wavelength of 32 0 nm and an emission wavelength of 400 nm 3. 3 .3 HPAEC Analysis of Monosaccharides (Notes 6–11) 3. 3 .3. 1 HPAEC ... the sample 3. 3 .3. 3 HPAEC SEPARATION OF SIALIC ACIDS (7) Connect a postcolumn solution of 30 0 mM sodium hydroxide at a flow rate of mL/min Follow steps 1–8 as described in Subheading 3. 3 .3. 1 Inject ... Different Derivatization Reagents Used for HPLC Separation of Monosaccharides Reference (33 ,34 ) (35 ) (36 ) (37 ) (37 ,38 ) (39 ) (40) (41,42) (27) ( 43) (6) (44) (45) (46) 177 178 10 11 12 Michalski and...

Ngày tải lên: 23/09/2012, 19:26

... 33 c 30 % acetonitrile over another 35 d Back to 20% acetonitrile over the next Store the column in 75:25 (v/v) acetonitrile:water 3. 7 HPAEC of Released Oligosaccharides 3. 7.1 HPAEC Analysis of ... periodate) for 40 at 0°C in the dark 3. 3 Release of Core Disaccharide (Galβ1-3GalNAcα–) from Mucin Using Endo-α-N-Acetylgalactosaminidase (O-Glycanase) 3. 3.1 The Removal of Glycerol from O-Glycanase ... instructions in the GlycoFree kit 3. 6 HPLC of 2-AB-Labeled Oligosaccharides 3. 6.1 Preparative HPLC on a GlycoSep C HPLC Column Wash the column with water for 30 at a flow rate of 0.4 mL/min Wash the...

Ngày tải lên: 23/09/2012, 19:26

... GlcNAc(β1–6)GalNAc-ol 4 .37 8 4.055 3. 534 4.244 3. 486 4.260 3. 984 ND 4.185 3. 490 4.242 3. 841 3. 379 4.021 3. 933 Klein et al Analysis of Mucin-Type O-Linked Oligosaccharides 195 Fig 1H-NMR spectra of three oligosaccharide-alditols ... Shifts of GalNAc-ol Residues Characteristic of the Nature of Oligosaccharide-Alditol Cores Gal(β1 3) GalNAc-ol 194 H-2 H -3 H-4 H-5 H-6' 4 .39 3 4.0 63 3.506 4.1 93 3.628 GlcNAc(β1 3) GalNAc-ol Gal(β1 3) [GlcNAc(β1–6)]GalNAc-ol ... Gal(β1 3) [GlcNAc(β1–6)]GalNAc-ol 4.286 3. 995 3. 546 4.141 ND NeuAc(α2–6)GalNAc-ol 4 .39 1 4.069 3. 468 4.277 ND 4.246 3. 846 3. 411 4.020 3. 532 Gal(β1 3) [NeuAc(α2–6)]GalNAc-ol H-2 H -3 H-4 H-5 H-6' GlcNAc(β1 3) [NeuAc(α2–6)]GalNAc-ol...

Ngày tải lên: 23/09/2012, 19:26

... re-equilibration in mM NaOH Measurement of Sulfate in Mucins 2 13 Fig Elution positions of acetate (CH3COO–), chloride (Cl–), sulfate (HSO4–), and phosphate (H2PO3–) ions (see also Note 11) Chromatogram ... analysis of inorganic ions The limit of detection of sulfate by ion chromatography as described here is about nmol Since mucins are often highly sulfated (i.e., the number of moles of sulfate ... >> the number of moles of protein), the amount of protein required for analysis will often be quite small For example, 10 pmol of a highly sulfated mucin (>100 mol sulfate/mol of protein) would...

Ngày tải lên: 23/09/2012, 19:26

... presence of Swiss mouse 3T3 feeders (approx × 104 cells/cm2) at 37 °C in a 5% CO2 in air incubator ( 13) In some situations, 3T3-conditioned medium (Subheading 2., item 5d) can be used instead of adding ... U/mL of insulin e Cell growth medium: Dulbecco’s modified Eagles medium (DMEM, Gibco/BRL, Parsley, Scotland) containing 100 IU/mL of penicillin, 100 µg/mL of streptomycin, 3. 7 g/L of NaHCO3, and ... cultures: DMEM, containing 10% FBS, 10 mM of sodium bicarbonate, mM of glutamine, 100 µg/mL of streptomycin, 50 IU/mL of penicillin, 50 µg/mL of gentamicin, and 20 mM of HEPES, pH7.2 b Submerged cultures:...

Ngày tải lên: 23/09/2012, 19:26

... radioactive precursors: D-(3H)glucosamine, 37 0–740 kBq, L-(3H)threonine, 37 0– 740 kBq or (35 S)sulphate 925 kBq In dual labelling experiments the ratio (3H):( 35 S) 37 0 kBq:925 kBq is used with ... each of the following labels used in pulse/chase labeling experiments: [35 S]methionine/ [35 S]cysteine (i.e., Promix™), [3H]threonine, [35 S]sulfate, and [3H]galactose—100 µCi (37 00 kBq) of label ... 2) are added: D-(3H)glucosamine, 37 0–1850 kBq, L-(3H)threonine, 37 0–1850 kBq, (35 S)sulphate 185–1850 kBq (see Notes 3 5) Cells are incubated under standard conditions for 4–96 h 3. 1.1.2 CONTINUOUS...

Ngày tải lên: 23/09/2012, 19:26