mcp cd55 and cd59 on the surface of lung epithelial cells increases after oxidative stress but this does not prevent complement activation on the cell surface
Báo cáo y học: " Some ABCA3 mutations elevate ER stress and initiate apoptosis of lung epithelial cells" pptx
Ngày tải lên :
12/08/2014, 13:22
... lysates of A549 cells expressing WT and R43L and R280C mutations, and only one protein band in the cells expressing L101P mutation (B) Deglycosylation assay with PNGaseF and EndoH on the membrane ... AECII Conclusion Apparently, the effect of ABCA3 mutations on homeostasis of lung epithelial cells depends on the type of the ABCA3 protein defect More work is necessary to verify if ER stress and ... that cause ER stress, but not other apoptotic stimuli [28,37] Cleavage of caspase leads to proteolytic activation of executioner caspase and to cell elimination Therefore, activation of caspase...
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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt
Ngày tải lên :
11/08/2014, 08:21
... the regulation of the expression of the known TLR adaptor proteins in small-airway epithelial cells To examine the impact of different TLR ligands on the expression of the adaptor proteins, cells ... emphasize the strong inflammatory properties of the TLR3 ligand poly(I:C) regarding the activation of lung epithelial cells that are likely to contribute to virus-induced exacerbations of pulmonary ... functions of the lung epithelium are critical for the local host defense providing protection of the airways and lung parenchyma from microbial colonization and infections Besides the induction of...
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Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione" pdf
Ngày tải lên :
12/08/2014, 18:20
... proliferation at high concentrations both in subconfluent layers of epithelial cells and during wound closure Effect of CSC on epithelial GSH content To investigate the effect of CSC on intracellular ... proliferation and its modulation by BSO and NAC on cell Effect of1 in NCI-H292 Effect of CSC and its modulation by BSO and NAC on cell proliferation in NCI-H292 Subconfluent cultures of NCI-H292 cells ... induction of apoptosis [13] In addition to ligands of the EGFR, oxidants have been shown to cause activation of EGFR [14] Therefore, oxidants may not only cause direct killing of epithelial cells, but...
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Báo cáo y học: "Construction of predictive promoter models on the example of antibacterial response of human epithelial cells" pdf
Ngày tải lên :
13/08/2014, 22:22
... new ideas The biological system we consider in this work is the transcriptional regulation of the response of lung epithelial cells to infection with Pseudomonas aeruginosa Binding of bacteria ... construction and show how these approaches work in the particular case of antibacterial response of a eukaryotic cell, namely the reaction of human lung epithelial cell to P aeruginosa binding One of the ... b-defensin gene expression in tracheal epithelial cells Infection and immunity 2000, 68:113-119 Diamond G, Jones DE, Bevins CL: Airway epithelial cells are the site of expression of a mammalian antimicrobial...
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Action of diclofenac and meloxicam on nephrotoxic cell death
Ngày tải lên :
26/09/2015, 10:59
... number of viable cells The production of purple formazan in cells treated with various concentrations of drugs was measured relative to the production in untreated control cells and the cell viability ... induction of MPTP allows for the activation and action of proteases and thus giving rise to the apoptosis phenotype On the other hand, Lemasters et al (1999) suggested that the progression to ... diclofenac 38 3.1.7 Inhibition of the intra-mitochondrial production of NAD(P)H by diclofenac 3.2 Action of diclofenac and meloxicam on kidney mitochondrial function 3.1.1 Uncoupling of oxidative...
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Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx
Ngày tải lên :
16/02/2014, 09:20
... among the regulation of nucleolin ⁄ C23, the activation of caspase-3 and the induction of apoptosis under the setting of oxidative stress and Hsp70 overexpression More importantly, for the first ... harvested and processed for annexin V–FITC and PI costaining and analysed by flow cytometry (A) Q3 cells were the control cells, Q4 cells were cells at the early apoptosis stage, Q2 cells were cells ... slides and examined by fluorescence microscopy Apoptotic cells were identified by the condensation and fragmentation of their nuclei The percentage of apoptotic cells was calculated as the number of...
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Báo cáo khoa học: "Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells" pptx
Ngày tải lên :
07/08/2014, 17:22
... 50,000 cells per well They were cultured for 24 h The medium was then replaced After rat lung epithelial cells were treated with various concentrations of PM2.5 and PM10 stock solution, the cytotoxicity ... at the same weight [18] The purpose of this study is not only to compare in vitro toxicity of PM2.5 and PM10 collected in urban area of Seoul in rat lung epithelial cell, but also to provide the ... reaction (RTPCR) of mRNA of repair enzymes To study the steady-state mRNA levels of the genes that could be induced by PMs, rat lung epithelial cells were treated with different concentrations of...
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Báo cáo y học: "Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro" potx
Ngày tải lên :
11/08/2014, 08:21
... 24 and 48 hours after treatment (Figures 3a and 3b) Since the number of detached cells varied depending on the treatment used, TUNEL positive cells are expressed as the number of cells per ml of ... peroxide and CSE (100%)(Figure 5) Discussion The aim of the present study was to investigate the effect of tobacco smoke and some of its components on apoptosis in A549 cells, an epithelial cell ... C and activation of Bax from the mitochondria h after treatment However, because few cells detached from the culture over this period, cytochrome C and Bax measurements were made on all the cells...
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Báo cáo y học: "Differential cytopathogenesis of respiratory syncytial virus prototypic and clinical isolates in primary pediatric bronchial epithelial cells" pdf
Ngày tải lên :
11/08/2014, 21:22
... bronchial epithelial cells (PBECs), derived from non-bronchoscopic brushings of children undergoing elective surgery [9] To address the question of whether the prototypic RSV A2 is representative of ... model is consistent with these reports In contrast, HS appears to be only present on basal cells in bronchial tissues or in well-differentiated (WD) PBECs, but is not found on apical cells [26,27] ... variability among individuals prevented these responses reaching significance Similarly, there was little or no evidence of RSV strain-dependence on the secretion of these analytes These data contrast...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
Ngày tải lên :
12/08/2014, 02:20
... mechanism of toll-like receptor (TLR) activation pathways that is intrinsic to the lungs Bronchial epithelial cells modulate the activation of monocytes, macrophages, dendritic cells (DC), and T ... in the stimulation of B lymphocytes for antibody production TNF-a together with IL-6 may boost proliferation and differentiation of B cells, and proliferation of T cells As a result, all these ... inflammatory response in lung tissues [55] Therefore, the exact regulatory role of this cytokine - TGF-b2, and its association with TLR activation in the initiation, progression, and resolution of immune...
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Báo cáo y học: "IL-13-induced proliferation of airway epithelial cells: mediation by intracellular growth factor mobilization and ADAM17" doc
Ngày tải lên :
12/08/2014, 15:20
... constitutively The majority of the growth factor (TGFα) was localized to the interior of the epithelial cells, with ample expression observed in the basal and central regions of the cells Little Cells ... expression particularly prominent in the apical region of the epithelial cells In fact, about 80% of control cells exhibited this pattern of expression which remained relatively unchanged as NHBE cells ... apical region /surface of NHBE cells are broad-reaching, having the potential to provide insight not only into the role of epithelial cells in allergic asthma, but also into the impact of intracellular...
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Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps
Ngày tải lên :
12/08/2014, 15:21
... http://respiratory-research.com/content/9/1/16 COX-2 Figure up-regulation mediated by TLR2 in epithelial cells in vitro and lung tissues of mice in vivo COX-2 up-regulation mediated by TLR2 in epithelial cells in vitro and lung ... kinase (MAPK) and nuclear factor (NF)-kappa B in lung epithelial cells The full understanding of the role of pulmonary endogenous anti-inflammatory mediators such as PGE2 and their regulation will ... However, whether NTHi infections have effect on the expression of COX-2 and subsequent PGE2 in lung epithelial cells still remains uncertain Lipid products of arachidonic acid including prostaglandins...
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Báo cáo y học: "Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complex" potx
Ngày tải lên :
12/08/2014, 15:21
... media of cells grown on Transwells The effect of NE on SLPI concentration in media of cells grown on Transwells The diagram shows the concentration of SLPI in each compartment of A549 cells grown ... concentration of SLPI Timing of the addition of inhibitor to NE affects concentration of SLPI The diagram shows the concentration of SLPI in the media of wells treated according to the conditions shown ... examine the relationship of NE concentration to the reduction of SLPI concentration in cell culture supernatants The initial concentration of SLPI in the cell supernatants was not known at the outset...
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Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
Ngày tải lên :
13/08/2014, 05:21
... http://www.retrovirology.com/content/5/1/86 Figure Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cells Secretion of IL-8 and CCL20 and Induction of cell surface ... infection of lung epithelial cells with HTLV-I increased the production of cytokines and chemokines and induced the surface expression of cell adhesion molecule http://www.retrovirology.com/content/5/1/86 ... cytoplasm of epithelial cells (A and D), and infiltrated lymphocytes (B and E) and macrophages (C and F) HTLV-I can be transmitted into lung epithelial cells from infected T cells and the integrated...
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The roles of shca proteins in response to oxidative stress 1
Ngày tải lên :
14/09/2015, 14:17
... Inhibition of ERK activation compromised H O -induced Akt activation and FOXO3a phosphorylation NIH3T3 cells were pretreated with different concentrations of U0126 before H2O2 was added Activation of ... 15 Figure 3.4A and B Phosphorylation of FOXO3a at Threonine32 induced by H2O2 involved ERKs activation (A) H2O2 treatment led to Akt activation and FOXO3a phosphorylation on Thr32 Cells were treated ... immunoprecipitated and Ser36 phosphorylation was determined by Western blot (D) Serum-induced activation of AKT and FOXO3a phosphorylation were not affected by inhibition of ERKs activation The experiments...
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The roles of shca proteins in response to oxidative stress 2
Ngày tải lên :
14/09/2015, 14:18
... Co-localization and translocation of PKC δ and ShcA in response to H2O2 treatment COS7 cells were transfected with PKC δ-GFP, p52shc, or both for 48 hours and then treated with H2O2 Cells were then fixed, ... 4.4 Cell fractionation studies of PKC δ and ShcA NIH3T3 cells were treated with H2O2 for 2.5, 5, 10 mins, collected and separated into cytosolic and particulate fractions The levels of PKC δ and ... and translocation of PKC δ and ShcA in response to H2O2 treatment COS7 cells were transfected with PKC δ-GFP, p52shc, or both for 48 hours and then treated with H2O2 Cells were then fixed, stained...
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Purification and studies on natural cellulase properties and produce recombinant cellulase from fungus in vietnam
Ngày tải lên :
04/02/2016, 10:18
... enhance the productivity of cellulase as a selection of strains capable of high cellulase synthesis, optimization of fermentation conditions to obtain large amounts of this enzyme Especially with the ... Triton X-100; TX-114: Triton X-114; ĐC: Control Additional Triton X-114 at a concentration of 1% (v/v) enhanced activity of the enzyme most strongly, but at a concentration of 1020% Triton X-114 ... in concentrations of 1-20% acetone; nbutanol and ethanol at a concentration of 1-5%; isopropanol in concentrations of 1-15% and high reliability for the detergents (Tween 20, Tween 80, Triton...
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Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf
Ngày tải lên :
15/03/2014, 23:20
... ethanol synthesis was absent in the rst 60 after O2 exposure, because of the strong inhibition of the ALDH activity of EhADH2 Furthermore, ALDH reactivation was observed, with the concomitant ... medium, and thus increased susceptibility to ROS in the absence of the proper cosubstrate In vivo inactivation and reactivation of fermenting enzymes and their effect on metabolic uxes Although the ... recovery of cluster integrity, and hence functional activity of the enzymes Regarding the reversible inactivation by H2O2 of EhPFOR, it might be possible that the concentration and incubation length...
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Báo cáo khoa học: "Early administration of IL-6RA does not prevent radiation-induced lung injury in mice" pot
Ngày tải lên :
09/08/2014, 08:23
... received only radiation of 21 Gy and confirmed that radiation-induced IL-6 production in Balb/c mice reached a peak week after irradiation (data not shown) The reason for the choice of this time ... various cells such as inflammatory cells responding to the release or activation of downstream cytokines, growth factors, or chemokines Anscher et al reported long-term (6-month) administration of the ... course of H&E and Azan staining in the lung tissue after irradiation is shown in Figures and 5, demonstrating that the extent and severity of lung damage was not significantly reduced in the IL-6RA...
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Báo cáo y học: " Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells" docx
Ngày tải lên :
12/08/2014, 14:20
... proliferation of epithelial and endothelial cells of various types, such as human endothelial cells [7], tracheal epithelial cells [8,9], retinal pigment epithelial cells [10] and thymic epithelial cells ... human endothelial cells [7], airway epithelial cells [8,9] retinal pigment epithelial cells [10], thymic epithelial cells [11] On the other hand, no such effects have been indicated on lung fibroblast ... hypothesis that CGRP proliferates alveolar epithelial cells, we have examined the effects of CGRP on the proliferation of alveolar epithelial cells and ERK signalling pathway as one of the cell...
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