maximum value and the number of times it occurs
Basic data structure trong lập trình
... write down the values of the elements in the corresponding leaves of the tree, then on the basis of these count values for the vertices of the previous level as the sum of the two leaves, then ... minimum / maximum) Minimum / maximum value and the number of times it occurs The task is similar to the previous one, but now in addition to the maximum required and return the number of its occurrences ... embodiment, the rank of a tree is the number of vertices in it, in the other - the depth of the tree (or rather, the upper limit to the depth of the tree, as the joint application of heuristics...
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Tài liệu Powerful Offline Marketing In The Internet Age ppt
... partner know from the start that: You’ve discovered an additional profit center for them that they are probably unaware of (offer projected profits, if possible) The additional profit center will ... by itself True, they are getting the contact info of some of their customers (something 101 Ways To Promote Your Business For Maximum Profits they themselves should be gathering), but if they ... business, there are experienced marketers out there who could handle the nuts and bolts of the campaign In other words, this would be the reverse of the previous two examples, where you are the professional,...
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Tài liệu Murray N. Rothbard - History Of Money And Banking In The United States (pdf) pptx
... judgments of value, with the choice of ends and of the means resorted to for the attainment of these ends, and with the valuation of the outcome of actions performed.21 Furthermore, whether directed ... definite judgments of value, chose definite ends, and resorted to definite means in order to attain the ends chosen, and it deals furthermore with the outcome of their actions, the state of affairs ... What was the net contribution of the railroad to the growth of real GNP in the United States? Or, what has been the effect of the creation of the Federal Reserve System on the stability of the price...
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Tài liệu Engineering Mechanics - StaticsChapter 1Problem 1-1 Represent each of the following combinations of units in the correct SI form using an appropriate prefix: (a) m/ms (b) μkm (c) ks/mg (d) km⋅ μN Units Used: μN = 10−6N kmμkm = 109−6Gs = 10 s pptx
... = 150 N Problem 2-17 Determine the magnitude and direction of the resultant force F R Express the result in terms of the magnitudes of the components F and F and the angle φ Solution: F R = F ... Problem 2-20 The plate is subjected to the forces acting on members A and B as shown Determine the magnitude of the resultant of these forces and its direction measured clockwise from the positive x ... clockwise from the positive x axis, so that the magnitude of force F in this chain is a minimum All forces lie in the x-y plane.What is the magnitude of F ? Hint: First find the resultant of the two...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
... inhibitor of CYPs These results suggest the specificity of the assay We did not observe any significant increases in the activity of mitochondrial CYP2E1 after supplementing the reaction with purified ... determinations of the supernatant containing resorufin were made at the excitation and emission wavelengths of 528 nm and 590 nm, respectively The ERND activities of mitochondria and mitoplast fractions ... [22] The nature and specificity of the endoprotease and the site of proteolytic cleavage remain unknown The same investigators were unable to see any significant intramitochondrial localization of...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... determined by ITC Values shown for affinity are those from the best fit of two sites nsd ¼ no site detected Values are in units of M)1 and are the averages of three measurements The standard error ... ion was followed using two isoforms of stefin B, one with E at site 31 (wildtype) and the other with Y at site 31 (variant 2), and the mutant P79S of the variant, in the three different buffers ... the maximal value of ThT fluorescence intensity was taken as 100% From these, for each reading of ThT fluorescence the percentage of inhibition of the fibril growth was obtained The percentage of...
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Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc
... expression of these genes depends on the presence of nicotine in the growth medium and we have determined the enzyme activities of the proteins Our results demonstrate the presence of two pathways of ... representation of the pAO1 gene and ORF cluster flanked by Tn554 and DTn The cluster consists of the pmfR gene, encoding the regulator of the purU-mabO-folD operon, a permease-like ORF, the genes of the ... function Arrows indicate the position of primers employed in the PCR amplification of gene fragments and the numbers the size in basepair of the amplified DNA fragment (B) RT-PCR of RNA derived from...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... independent of the thermodynamic and kinetic properties of the binding process and is solely based on the detectability of the peptides of interest We have used this method to show that binding of fosfomycin ... ligand binding The loop region of the upper domain moves towards the active site of the enzyme, thus shielding the substrates (and ligands) from bulk solvent A detailed thermodynamic study of ... entry 1UAE [11]), conformation The thiol group of C115 is highlighted in green and the nitrogen atoms of R120 and K22 in blue The open form of MurA is unliganded (A) and the closed conformation (B)...
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Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
... (Fig 3A) On the basis of the Endo H digestion (Fig 2B,C), the large band is assigned to proteins with N-glycans of mature complex type and the small band to the proteins with N-glycans of mannose-rich ... represent the exponential fit with half-lives of 22 and 23 h for wild type GAT1 in the absence and presence of dMM, respectively, and of 12 and 5.5 h for the DDN and DDQ mutants, respectively GABA values ... important for the GABA transport activity 1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role of the terminal structures of the N-glycans of GAT1,...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... aconitase accumulate in the housefly mitochondria with age accompanied by loss in its activity [17,44] Nevertheless, the modifications we observed did not affect the ATPase binding site of the ... membrane The decrease in enzymatic Lon protease activity may be the result of either a loss of efficiency in relation to damaged protein and/ or a decrease in the amount of this protein in the matrix ... modified proteins even in the mitochondrial matrix of adult rats, the concentration of which increased significantly with aging On the one hand we found evidence of the occurrence of oxidative protein...
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Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
... filtration without the addition of ATP (white bar, lane 1), in the presence of 160 pmol dWB ClpA6 without (lane 2) or with addition of mM ATP (lane 3), or in the presence of 160 pmol RR ⁄ dWB ClpA6 without ... dependent on the global thermodynamic stability of the substrate, Substrate recognition and unfolding by ClpA but rather correlates with local unfolding of the substrate (i.e unfolding of the N- or ... a common fold with the N-domain of ClpA) in substrate selection [15–17] One difficulty in understanding the role of the N-domain of ClpA stems from the variety of activities exhibited by various...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... mol) The results are mean ± SD, n ¼ 1–4 The degree of permeabilization is expressed as the percentage of the maximal value obtained at the end of the assay by the addition of mM Triton X-100 The ... where it is native and predominantly dimeric [20], exhibits some toxicity This might be due to the inherent toxicity of lower oligomers or it could be due to the influence of the low pH at the membrane ... pH 3.3 bound the most of all, as the signal increase during the injection phase was the largest and there was low dissociation after the end of injection Discussion The main hypothesis for pathology...
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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
... contrast to the three classes of antagonist, the binding of AVP was dramatically affected by the substitution of Arg46, with the affinity of AVP for all Fig Schematic diagram of the V1aR The V1aR ... to maintaining the conformational switch of the V1aR in the off-state The unique ability of arginyl to fulfil this constraining function in the N-terminus of the V1aR is similar to the unique constraining ... increased for all 19 substitutions compared to the wildtype Arg46, with the precise value depending on the identity of the substituent amino acid (Fig 5) These data establish that the mutant receptors...
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Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf
... occupancy of the nucleotide-binding site could alter the accessibility and reactivity of this cysteine Therefore, the purified singlecysteine-containing fusion proteins were examined for their ability ... to asymmetry in these proteins The crystal dimer of the NBD of the maltoseuptake system of Thermococcus littoralis (MalK) consists of two monomers with deviation from perfect twofold symmetry ... properties of the NBDs, rather than of their interactions with the TMDs (e.g [50] and see introduction for further references) Recent structural determinations of both intact ABC transporters and NBD...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
... and the intensity of each band was determined using the NIH Image software The enzymatic activity of the P-DN42 and mutant P-DN42 gene products was determined as described below The activity ... the medium was quantified by Western blotting in combination with densitometric scanning of the bands on the blotting membrane The enzymatic activities were correlated with the concentration of ... to 40% of the parent enzyme activity In a more in-depth evaluation of the function of the three serine mutants, the kinetic properties of the mutant enzymes were compared to those of the parent...
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Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot
... similar position as the lowest faint band observed for wild-type Kv3.1 and the lower faint band of the single mutants, and furthermore, the immunoband did not shift in the presence of TM To verify ... to the lower two immunobands of wild-type Kv3.1 In both cases, the upper band was darker than the lower band Additionally, the upper band was not visible in the presence of TM A single immunoband ... expressed in Sf9 cells The upper band was the predominant band, and the two lower bands were of minor intensity (Fig 3) In the presence of PNGase F, Endo H, or TM, the upper band was no longer observed...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
... factors and the )10 and +90 sites Efficient initiation requires at least one of the two sites It appears that the )70 Sp1 motif is required whether initiation is directed by either of the )10 ... activities were compared using the concentration of protein in the extract as an internal standard The activity of ()119/+178) PS1CAT in the presence of the empty vector was taken as 100% The values ... flexibility in the mechanism of initiation presents several advantages First it is consistent with the ubiquitous expression of the gene and increases its potential for expression in a variety of...
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Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx
... introduction of Gln for Leu at position 28 does not increase the stability or significantly affect the structure, in spite of the greater hydrophilicity and helical propensity of Gln However, the presence ... a thermal stability higher than that of mBS However, P19A/L28Q-mBS has the same thermal stability of the parent protein, in spite of greater similarity to RNase A To directly characterize the ... residues of the active site (His12 and His119) [11], which belong to the same subunit in M¼M and to different subunits in MxM Thus, upon reduction and denaturation, either a product of molecular...
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