matrix metalloproteinases in tumor progression

... expression in diverse tumor types, suggesting that these proteins are involved in different steps of cancer progression including carcinogenesis [9,10] ADAM molecules are implicated in tumor cell ... solid tumors [20] During cancer progression, increased shedding of the membrane-anchored ligands of EGFR, including heparin-binding EGF (HB-EGF), transforming growth factor (TGF)-a and amphiregulin, ... molecules, integrins and interacting with phosphorylation sites for serine ⁄ threonine and tyrosine kinases, thus contributing to cancer development [11] Roles of MMPs in cancer progression During development...

Ngày tải lên: 15/03/2014, 00:20

... primary tumor [102] The possible involvement of heparanase in tumor selfseeding is supported by the timing of its induction during tumorigenesis and its prometastatic function Using the RIP-Tag2 tumor ... protein-coding genes contain introns that are removed in the nucleus by RNA splicing and are often alternatively spliced Alternative splicing increases the coding capacity of the genome, generating ... determinant behind its signaling capacity The existence of a C-terminus domain (C-domain) emerged from a prediction of the 3D structure of a single-chain heparanase enzyme [73] In this protein 3894...

Ngày tải lên: 15/03/2014, 23:20

... expression in the hippocampus is involved in Abinduced cognitive dysfunction [10] These findings together point to the need to increase our understanding of the role of MMPs in AD and their relation ... within each group and to define ‘individuals within the control group with increased risk for future AD’ The group difference in the CSF analysis method should therefore not influence the findings ... of matrix metalloproteinases in the central nervous system J Neurosci Res 2003, 74:801-806 Asahina M, Yoshiyama Y, Hattori T: Expression of matrix metalloproteinase-9 and urinary-type plasminogen...

Ngày tải lên: 22/03/2014, 14:20

... ovarian tumors [16] According to these findings, MMPs might play a critical role in the first steps of tumorigenesis in ovaries Surprisingly, to our knowledge, no single study to date investigated ... carcinomas corresponds to our finding of strong mRNA expression in Caski and SiHa cell lines [13] Further, we found a strong expression of MMP-15 mRNA in those cell lines These results are in line ... their expression analysis in general These cell lines are also promising candidates for future investigations dealing with the role of MMPs in tumor invasion and building of metastatic formations...

Ngày tải lên: 28/03/2014, 14:20

... critical zinc-binding region in the catalytic domain that resulted in markedly decreased transcription of the MMP13 gene and no apparent translation Recombinant MMP13 lacking this catalytic domain has ... substitution of an arginine by a histidine (R101H) In affected members of both families there was no MMP-2 activity detected in serum by gelatin zymography, in contrast to measurable levels in unaffected ... I, Hart DA: Matrix metalloproteinase-13 expression in rabbit knee joint connective tissues: influence of maturation and response to injury Matrix Biol 2000, 19:431-441 Coussens LM, Fingleton B,...

Ngày tải lên: 09/08/2014, 01:21

... Discussion PKR is a serine/threonine protein kinase that can phosphorylate a limited number of cellular proteins, including the eukaryotic initiation factor 2α (eIF2α), resulting in a block on translation ... inhibitors of matrix metalloproteinases Crit Rev Eukaryot Gene Expr 1997, 7:159-178 Mengshol JA, Vincenti MP, Coon CI, Barchowsky A, Brinckerhoff CE: Interleukin-1 induction of collagenase (matrix ... containing 10 IU penicillin and 10 µg/ml streptomycin In vitro cartilage samples Cartilage was taken from the metacarpophalangeal joint of 7-day-old bovine calves within 12 hours of slaughter using...

Ngày tải lên: 09/08/2014, 01:23

... are induced in host cells in response to infectious stimuli Normally, MMPs assist in clearing infections, initiating immune responses, and in tissue remodeling [9] Excessive MMPs cause matrix degradation ... Elkington PT, O'Kane CM, Friedland JS: The paradox of matrix metalloproteinases in infectious disease Clin Exp Immunol 2005, 142:12-20 Burrage PS, Mix KS, Brinckerhoff CE: Matrix metalloproteinases: ... during S aureus infections Calander and colleagues [29], using wild-type S aureus strain 8325-4 and its mutants lacking aureolysin, serine protease, and cysteine protease, demonstrated in a murine...

Ngày tải lên: 09/08/2014, 08:23

... cell–MMP interactions have been reported in recent years, such as the binding of gelatinase-A to the integrin αvβ3 [33], binding of gelatinase-B to CD44 [28], and binding of Matrilysin reports ... progressively and intense staining for matrilysin is observed in these migrating cells, especially those in close contact with the underlying matrix Release of matrilysin towards the matrix was seen in association ... methionine to the carboxy side of the zincbinding site In the inactive state, the conserved cysteine residue in the pro-domain provides the fourth coordination site for the catalytic zinc ion,...

Ngày tải lên: 12/08/2014, 18:20

... protein IL-2 Interleukin-2 IL-2Rα Interleukin-2 receptor alpha IL-4 Interleukin-4 IL-5 Interleukin-5 IL-6 Interleukin-6 IL-8 Interleukin-8 IL-10 Interleukin-10 IL-1β Interleukin-1 beta IL-12 Interleukin-12 ... metalloproteinases (TIMPs) 16 I Matrix metalloproteinase inhibitors 17 J Matrix metalloproteinases in the normal lung 19 K Matrix metalloproteinases in cancer .19 L Matrix metalloproteinases ... beta IL-12 Interleukin-12 IL-13 Interleukin-13 xxii IL-21 Interleukin-21 IL-22 Interleukin-22 IL-23 Interleukin-23 IFN-γ Interferon gamma ITK IL-2 inducible T cell kinase IP3 Inositol 1, 4, triphosphate...

Ngày tải lên: 24/08/2014, 11:37

... tumor progression 96 Figure 23: Diagram illustrating the progression of tumors in RETAAD mice 99 Figure 24: In silico modeling of tumor growth in non-vascularised tumors ... to TAMs in other parts of the tumor [8,30] In summary, inflammation and the immune system play critical roles in both tumor suppression and tumor progression Understanding the complex interactions ... analysis Tumors were dissected from RETAAD mice and cut up into small pieces in RPMI medium without serum Single cell suspensions were obtained by incubating the tumor pieces for 20mins at 37°C in...

Ngày tải lên: 10/09/2015, 15:50

... disseminated cancer cells by 80% (reduction seen in primary tumor) Therefore (4) 𝑘𝑘 𝑑𝑑 = 0.2𝑘𝑘 𝑐𝑐 By combining (2), (3) and (4), one finds (5) 𝐷𝐷𝐷𝐷𝐷𝐷 𝑐𝑐 � 𝐷𝐷𝐷𝐷𝐷𝐷 = 𝑑𝑑 Therefore, even an 80% inhibition ... 2: Immune profiling gating strategy Identification of immune subsets in single-cell suspension of RETAAD tumors The figure shows the strategy used to enumerate immune cells in tumors E Appendix ... 5: Alternate inhibitors of HGFR, EGFR and TGF-βR1 Alternative Inhibitors block PMN-MDSC induced EMT Alternative inhibitors block PMN-MDSC induced EMT in NBT-II cells AG1478 – EGFR inhibitor, SGX523...

Ngày tải lên: 10/09/2015, 15:50

... intoxication with genotoxins, may allow cells carrying genomic instability to break through the tumorigenesis barrier, resulting in an increased risk of tumor development vated protein kinase [57] (Fig ... pro -in ammatory cytokine production, there is now evidence linking T cell-mediated immune responses in infection-induced carcinogenesis The production and secretion of the B fragilis toxin induces ... gain of chromosomes) 72 h post-infection Such aberrations were maintained in a proportion of cells up to 21 days postinfection The chromosomal instability induced by infection with colibactin-producing...

Ngày tải lên: 14/03/2014, 22:20

... and link metalloproteinases (MMPs), tisleukin (IL)-1 on the expression of matrix protein in (TNF)-α and/or interleukin (IL)-1 on the expression of matrix metalloproteinases (MMPs), tissue inhibitors ... link protein, chain in Figures and derived as described in Materials Polymerase and ADAMTS analysis,7 matrix metalloproteinase (MMP), link protein, and ADAMTS analysis, derived as described in ... symptoms and progression of OA, it is an important question which cell type in the OA synovium is responsible for maintaining synovial inflammation In RA, in which the macrophage is the main promoter...

Ngày tải lên: 09/08/2014, 08:23

... signalling [38] and results in increased apoptotic activity Involvement of TIMPs in the regulation of death pathways including Fas/Fas ligand is an interesting finding, because alterations in Fas ... stage, a cysteine residue within the prodomain interacts with zinc to prevent substrate binding The haemopexin domain mediates interaction with enzyme substrates Specific to the gelatinases is the ... (a disintegrin and metalloproteinase) families of matrix metalloproteinases [35] One possible basis for these functions is the fact that certain ADAMs and MMPs are involved in the shedding of...

Ngày tải lên: 09/08/2014, 13:22

... Gelatinase-B 20q11.2-q13.1 vitronectin,decorin,plasminogen Gelatin,CollagenI,IV,V,VII,X,XI,XVII I,vitronectin,Elastin,laminin,fibro nectin, ProMMP-9 proMMP-2 MMP-3 Stromelysins-1 11q22.2-22.3 Laminin, ... Elastin, gelatin, collagen I,IV, fibronectin, laminin, vitronectin, proteoglycan MMP-7 Matrilysin-1 11q22.2-22.3 Collagen I,IV,V,IX,X,XI,XVIII, Fibronectin,laminin,gelatin,aggr egan,,gelatin,proMMP-9 ... Laminin, aggregan gelatin, fibronectin MMP-10 Stromelysins-2 11q22.2-22.3 CollagenI,III,IV,gelatin,elastin,pro MMP-1,8,10 MMP-11 Stromelysins-3 22q11.2 Fibronectin,laminin,aggregan,gel atin MMP-12...

Ngày tải lên: 10/08/2014, 05:21

... Matrix metalloproteinases (MMPs) constitute a large family of proteolytic enzymes containing a metal in their organic structure playing key roles in degradation of proteins in extracellular matrix ... Jr: Matrix metalloproteinases J Biol Chem 1999, 274:21491-94 Matrisian LM: The matrix degrading metalloproteinases Bioessays 1992, 14:455-463 Murphy G, Nagase H: Progress in matrix metalloproteinases ... Expression of matrix metalloproteinases in patients with acute myocardial infarction Jpn Circ J 2001, 65:71-75 Toshiharu I, Noriko A: Collagen and elastin degradation by matrix metalloproteinase in aortic...

Ngày tải lên: 10/08/2014, 10:20

... procollagenase by matrix metalloproteinase (stromelysin) Biochemistry 1990, 29:1026110270 28 Brown PD: Synthetic inhibitors of matrix metalloproteinases In Matrix Metalloproteinases Edited by ... Windsor LJ, Birkedal-Hansen B, DeCarlo A, Engler JA: Matrix metalloproteinases: a review Crit Rev Oral Biol Med 1993, 4:197-250 23 Woessner JF: The matrix metalloproteinase family In Matrix metalloproteinases ... NE; lane 3: indomethacin; lane 4: NE + indomethacin; lane 5: PGE2; lane 6: NE + PGE2; lane 7: NE + indomethacin + PGE2 (c) BM alone Lane 1: control; lane 2: NE; lane 3: indomethacin; lane 4:...

Ngày tải lên: 12/08/2014, 18:20

... years; 73% men) were included Matrix metalloproteinases (MMPs) are a family of zinc-containing endoproteinases implicated in degradation and remodelling of the extracellular matrix (ECM) They can ... = interleukin; MMP = matrix metalloproteinase; TIMP = tissue inhibitor of matrix metalloproteinase; TNF = tumor necrosis factor Inflammatory status was assessed in sepsis patients by measuring ... Activated partial thromboplastin time; INR = International normalized ratio; MMP = Matrix metalloproteinase; TIMP = Iissue inhibitor of matrix metalloproteinase; IL = interleukin; rho = Spearman's rank...

Ngày tải lên: 13/08/2014, 19:20

... aggressively investigating biomarkers of disease and injury In the scene of sepsis biomarkers, C-reactive protein, IL-6 and procalcitonin are the most investigated markers in clinical trials In recent ... Immunol 2001, 22:571-579 Elkington PTG, O`Kane CM, Friedland JS: The paradox of matrix metalloproteinases in infectious disease Clin Exp Immunol 2005, 142:12-20 Pugin J, Widmer MC, Kossodo S, ... clinical evidence, for example triggering receptor on myeloid cells or N-terminal pro-brain natriuretic peptide [14,15] Unfortunately, biomarkers rarely alter our clinical decision-making in...

Ngày tải lên: 13/08/2014, 19:20

Ngày tải lên: 13/08/2014, 20:21