materials small rna library construction and deep sequencing

... strand, demonstrating the strand-specificity of our library [15] (Materials and methods) We next combined these reads to define consecutive regions of strand-specific transcription (Materials and ... antisense to NOP10) Shown are reads mapped from a standard cDNA sequencing library [15] (yellow), and from the strand-specific library prepared and run side-by-side on the same flow cell (green: ... used strand-specific RT-PCR and sequencing to validate the presence of the orthologous sense and antisense transcripts in each species in mid-log and early stationary phase, and used strand-specific...

Ngày tải lên: 09/08/2014, 20:22

... albumin and hsCRP, and the hazard of death was non-linear (p < 0.10), and there was evidence that the association between albumin and the hazard of the combined endpoint of mortality and loss ... CN, EK, IZ and LM were responsible for study design and data collection MB, BS and JK performed the statistical analyses JC and EK performed the laboratory analyses JK, MB, EK, CW, JC and AM drafted ... albumin, and 58 hsCRP and ferritin values) using multiple imputation techniques [19] The proportion of imputed values was within the commonly accepted range [19] Koethe et al Journal of the International...

Ngày tải lên: 20/06/2014, 08:20

... relationship to joint and vascular damage and risk factors in RA patients [19] We report associations of each protein with both joint and vascular disease and their risk factors Materials and methods Subjects ... between and 14 kDa, and are characterized by two calcium binding sites of the EF-hand type (helix-loop-helix) [20] S100A8 and S100A9, generally functioning as the S100A8/A9 heterocomplex, and S100A12 ... design, acquisition, analysis and interpretation of data WY and CG carried out S100A8, S100A9 and S00A12 assays YC, CG, MB and RT wrote the manuscript All authors read and approved the final manuscript...

Ngày tải lên: 09/08/2014, 13:22

... pre-heparinized needle and syringe and the birds were killed by intravenous injection of sodium pentobarbitone Blood samples were centrifuged for at 1500 × g and the plasma supernatant was frozen and stored ... h at 4°C and then both breast muscles (m pectoralis) and both whole thighs (bone and muscles) were cut from the carcass and evaluated for muscle colour, lightness (L*), redness (a*) and yellowness ... in a cold muffle furnace and heated to 550 ± 5°C for 16 h (overnight) After this time, the beakers and their contents were removed from the furnace, placed in a desiccator and allowed to cool...

Ngày tải lên: 14/08/2014, 13:21

... social class and socioeconomic factors like childhood poverty and family income, low parent education, and the more limited opportunities and negative influences faced by minority children And indeed, ... differences hold up after controlling for family income and poverty, low parent education levels, and race and ethnicity An intact two-parent family and regular church attendance are each associated ... Administration of Children and Families (ACF) of the U.S Department of Health and Human Services This involved designing and field testing one-on-one child assessments and developing a computer-based...

Ngày tải lên: 28/03/2014, 09:20

... changes in mood and behavior were to be considered, novel codes and standards that influence the choice of lighting technologies and the design of indoor environments could be developed and implemented ... cluster sampling design was planned by Statistics Finland The sampling frame comprised adults aged 30 years and over living in mainland Finland This frame was regionally stratified according to ... scores of to assigned as low and those of to 36 as high) Seasonal changes in mood and behavior Seasonal changes in mood and behavior were measured using items taken and adapted from the SPAQ [13]...

Ngày tải lên: 18/06/2014, 19:20

... (quality of life and distress and urinary incontinence) and search (quality of life and bother and urinary Results of search (quality of life and distress and urinary incontinence) and search (quality ... The first search used the terms "quality of life and distress and urinary incontinence" and the second used the terms "quality of life and bother and urinary incontinence." The searches were limited ... incontinence episodes and total UDI and IIQ scores [55] Relationships between UDI and IIQ were examined at baseline and post-operatively One study reported a moderate correlation between UDI and IIQ at...

Ngày tải lên: 18/06/2014, 19:20

... laboratory tests and wrote the manuscript; RS and GP carried out RT-PCR tests; JM performed the sequencing experiments; AF, KZ, RB and DS supervised the rotavirus surveillance program and critically ... Conclusion RNA extraction The QIAamp Viral RNA mini kit (Qiagen/Westburg, Leusden, The Netherlands) was used according to the manufacturer's instructions for the extraction of rotavirus RNA from ... (14.6%), and G4 (13.8%) G1 comprised only 11.6% of the isolates and 36.9% of the rotavirus-positive samples were untypeable VP7 gene sequence analysis We amplified the VP7 genes of five randomly...

Ngày tải lên: 19/06/2014, 08:20

... triglycerides and lower LDL than healthy controls [20] However, only limited information is available on the effect of serum triglycerides and cholesterol levels and the roles of HDL and LDL levels ... lipid profile and clinical status The standardized acquisition and analysis methods for obtaining contrast-enhancing lesion volume (CE-LV), CE lesion number (CEL number), T2-LV, T1-LV and brain parenchymal ... EDSS < 4.0 and ≥ 4.0 The baseline EDSS groups were analyzed using logistic regression with sex as a factor and disease duration and lipid profile variable of interest The CE-LV, T2-LV and T1-LV...

Ngày tải lên: 19/06/2014, 22:20

... GF, ZsI and PG conceived of the study, and participated in its design and coordination, and helped to draft the manuscript; L-MT and M-OS carried out the immunoassays; GSZ, T, GP, KH, RSZ and ZSz ... ficolin-3 and high CRP levels on the one hand and the unfavorable outcome of the disease on the other hand, we repeated the analysis as above in the follow-up samples by including ficolin-3 and CRP ... ficolin-3 on the one hand and the severity of ischemic stroke and the infarct size on the other hand, as discussed above It is well known that both the high baseline NIHSS score and the high serum...

Ngày tải lên: 19/06/2014, 22:20

... lineage III (blue) and lineage IV (red) lected to understand this complex epidemiology [15] However, in recent years it was reported that the accumulation of point mutations in VP4 and VP7 genes was ... [17,22-24] and one for P-typing [25] In the one developed by Gouvea et al [24], it was reported failures in the correct genotyping of G2, G8, G9 and G10 rotavirus strains [7,12,17,20,21], and In ... corrections of the manuscript, and Juan Stupka and the three anonymous referees for critical corrections 20 References Parashar UD, Gibson CJ, Bresee JS, Glass RI: Rotavirus and severe childhood diarrhea...

Ngày tải lên: 20/06/2014, 01:20

... laboratory tests and wrote the manuscript; RS and GP carried out RT-PCR tests; JM performed the sequencing experiments; AF, KZ, RB and DS supervised the rotavirus surveillance program and critically ... Conclusion RNA extraction The QIAamp Viral RNA mini kit (Qiagen/Westburg, Leusden, The Netherlands) was used according to the manufacturer's instructions for the extraction of rotavirus RNA from ... (14.6%), and G4 (13.8%) G1 comprised only 11.6% of the isolates and 36.9% of the rotavirus-positive samples were untypeable VP7 gene sequence analysis We amplified the VP7 genes of five randomly...

Ngày tải lên: 20/06/2014, 04:20

... conceptualized and designed the experiments, collected, assembled, analyzed and interpreted data, and drafted the manuscript OC conceptualized and designed the experiments, and collected, assembled and ... designed and implemented experiments SS recruited, collected and annotated specimens, and interpreted data KO recruited, collected and annotated specimens, and interpreted data SC collected and annotated ... specimens, and analyzed and interpreted data PD conceptualized and designed the experiments, analyzed and interpreted data, and helped with the drafting of manuscript JM conceptualized and designed...

Ngày tải lên: 20/06/2014, 08:20

... the internode and petiole angles and azimuths, the angle and azimuth of the leaf surface normal, and the azimuth of the midrib were recorded with a compassprotractor Leaf, petiole, and internode ... and bibliography of Fagales (Betulaceae, Corylaceae, Fagaceae and Ticodendraceae), The Royal Botanic Gardens, Kew, 1998 [15] Grassi G., Giannini R Influence of light and competition on crown and ... Photoinhibition and drought in Mediterranean woody saplings: scaling effects and interactions in sun and shade phenotypes, J Exp Bot (2005) 56: 483–494 [49] Waller D.M., The dynamics of growth and form...

Ngày tải lên: 07/08/2014, 16:20

... endostatin and bFGF and other clinical parameters No correlation of VEGF, endostatin and bFGF levels with skin score, carbon monoxide diffusion capacity and the presence of teleangiectasias and other ... (median, 453 pg/ml; range, 93–1143 pg/ml) and patients without detectable autoantibodies (P = 0.36) Autoantibodies and bFGF and endostatin levels Disease duration and VEGF levels To examine whether ... (Scl-70 neg) and higher levels than patients without detectable autoantibodies Data are shown as box plots, with upper and lower quartiles shaded # P < 0.05 Capillaroscopy and endostatin and bFGF...

Ngày tải lên: 09/08/2014, 06:22

... and clinical findings, the objective of this study was to explore the relationships of KRAS and BRAF status with p-AKT and p-ERK expression and with clinical outcomes (response to radiation and ... http://www.ro-journal.com/content/6/1/114 phosphorylation (p-ERK) and activation [14] ERK is required for cell proliferation and allows for the evasion of apoptosis [14] To date, only small studies ... of paraffin Pyrosequencing was performed to identify KRAS mutations in codons 12, 13 and 61 and the BRAF V600E mutation using the PyroMark Q96 KRAS v2.0 kit (#972452, Qiagen) and PyroMark BRAF...

Ngày tải lên: 09/08/2014, 09:21

... Surgery and Internal Medicine, the nurses and physician assistants at the Department of Gastroenterology, the research nurses at the Department of Rheumatology and Clinical Immunology, and the ... writing the article HEV and RWF also contributed to the selection and inclusion of cases and controls JvdP also contributed to the statistical analysis of the data All authors read and approved the ... actual and recent medication, co-morbidity and medical history The questionnaire contained specific items on the use of NSAIDs, aspirin, anticoagulants, gastroprotective drugs, and steroids, and...

Ngày tải lên: 09/08/2014, 10:20

... activated T and B cells [16-21]; altered proportions of naïve and memory T and B cell populations [17,21-23]; and deficiencies of regulatory T-cell subsets such as natural killer (NK)T [24,25] and T-regulatory ... cells, NK cells, and CD56+ T cells, and had significantly smaller sample sizes Clark and coworkers [35] examined antibody-secreting cells in 25 first-degree relatives of lupus patients and found similar ... NKT cell numbers and function, and that these changes precede the development of disease Aside from the changes in NKT cell numbers and the proportions of memory and/ or naïve B and CD4+ T cells,...

Ngày tải lên: 09/08/2014, 13:21

... 0.965 and Correlation coefficient r = 0.872 for rs6990375 G>A and rs3802278 G>A; D’ = 0.981 and r2 = 0.678 for rs6990375 G>A and rs3087714 C>T; D’ = 1.000 and r2 = 0.919 for rs3802278 G>A and rs3087714 ... (Promega, Madison, WI) and T4 DNA ligase (New England BioLabs) were used for ligation Transient Transfection and Luciferase Reporter Gene Assay The ovarian cancer cell lines OVCA429 and SKOV-3 were ... survival, and promoter activity (A) The gene structure, SNP location, predicted functionality of SNPs, and electrophoresis gel pictures; (B) Haplotype combination of rs2623047 and rs6990375 and age...

Ngày tải lên: 10/08/2014, 10:20

... pro-inflammatory cytokines and inhibit iNOS expression and NO release in LPS-stimulated RAW264.7 macrophages by targeting the NFB activation pathway Materials And Methods Cell Culture and Sample Treatment ... Brodsky et al Journal of Inflammation 2010, 7:3 http://www.journal-inflammation.com/content/7/1/3 Page of Figure Effect of AS101 on LPS-induced iNOS protein expression (A-D) and NO(E) and IL-6 (F) ... experiments and carried out the majority of the assays, performed the statistical analysis, participated in the design and coordination of the study, and drafted the manuscript Brodsky et al Journal...

Ngày tải lên: 11/08/2014, 08:22