material growth conditions and rna extraction
báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
Ngày tải lên :
12/08/2014, 03:20
... both rice and Arabidopsis thaliana, and are distributed non-randomly throughout the genome [30-32] Clustering of R genes is known to facilitate tandem duplication of paralogous sequences and generation ... RNBS-II, RNBSIII, GLPL and RNBS-V) (Additional File 5) and were compared to those from closely related Poaceae species (wheat and rice) and to A thaliana The P-Loop, Kin-2A and GLPL motifs were ... or lacked CC and/ or LRR domains P-Loop, Kin-2A and GLPL motifs were conserved and similar in sequence to those of closely related Poaceae species such as wheat and rice [31,58] and the model...
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Báo cáo y học: "Validation and refinement of gene-regulatory pathways on a network of physical interactions" pps
Ngày tải lên :
14/08/2014, 14:21
... Vertical bars indicate the standard deviations for the random selection method The standard deviations for the information and hub selection curves are less than five and are not shown for clarity ... hybridizations Total RNA was isolated by hot acid phenol extraction, purified to mRNA (Ambion PolyAPure kits), and labeled with Cy3 or Cy5 by direct incorporation (Amersham CyScribe First-Strand cDNA Labeling ... 'coherence'; details of the computation of expression coherence and the selection of the gene sets are described further in Materials and methods and [23] As shown in Figure 3a, the gene set downstream...
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Báo cáo y học: "he discovery, positioning and verification of a set of transcription-associated motifs in vertebrates" pps
Ngày tải lên :
14/08/2014, 15:20
... promoter and the expression patterns were scored in a double-blind manner (see Materials and methods) All three promoters showed some ubiquitous expression and, for two of the genes (Q99JW1 and Q96BU7), ... real and random promoterwise alignments select this threshold We performed simulation studies with random sequence that showed that bit scores >20 bits are extremely rare when aligning randomly ... space and time efficient manner, we developed a simpler approach along the lines of the same greedy approach (see Materials and methods) lists these 55 motifs (some occur in both the CpG and the...
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báo cáo hóa học:" Cross-cultural validation and analysis of responsiveness of the QUALIOST®: QUAlity of Life questionnaire In OSTeoporosis" pptx
Ngày tải lên :
20/06/2014, 15:20
... consent and the protocol was approved by the Ethics Committee Baseline and annual X-rays were provided and assessed by a centralised procedure (Pr C Roux, Cochin Hospital, France) The prevalent and ... its first version had 168 questions and the Osteoporosis Functional Disability questionnaire (OFDQ) [4] focused mainly on pain and handicap rather than QoL and was not generated using patient ... (M0) and then every six months using the SF-36 and QUALIOST® The 12 countries of the SOTI trial were Australia, Belgium, Denmark, France, Germany, Greece, Hungary, Italy, Poland, Spain, Switzerland...
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HIV INFECTION IN THE ERA OF HIGHLY ACTIVE ANTIRETROVIRAL TREATMENT AND SOME OF ITS ASSOCIATED COMPLICATIONS docx
Ngày tải lên :
27/06/2014, 18:20
... Neuropathies Katrin Hahn and Ingo Husstedt Chapter Spondylodiscitis and HIV – Diagnosis and Treatment Strategies 121 Jan Siewe, Kourosh Zarghooni and Rolf Sobottke Chapter Acute Abdomen and HIV Infection ... diabetes, and use of lipid lowering and hypertension therapy 20,775 HIV-infected and 215,158 HIV negative members contributed to 90,961 and 1,133,333 persons-years (py) respectively HIV-infected and ... transcriptase inhibitor (PIs/NNRTI) regimens (54 and 44% respectively), followed by PIs regimes (40 and 27% respectively) and NNRTI-containing combinations (32 and 23% respectively) (Friss Moller et al.,...
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Báo cáo y học: "Perspectives and limitations of gene expression profiling in rheumatology: new molecular strategies" doc
Ngày tải lên :
09/08/2014, 01:23
... and will reduce our screening and analysis efforts to the principal components and actors Strategies Suggestions by Firestein and Pisetsky [41] underline the importance of an understandable and ... retroviral transcripts in renal tubules and also in brain and lung tissue Azuma and colleagues used microarrays for the detection of new candidates in salivary gland tissue from the MLR/MpJ-lpr/lpr ... invasion and bone resorption, also demand the identification of targets to directly inhibit destruction and/ or to induce regeneration and repair A deeper knowledge of pathophysiological networks and...
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Báo cáo y học: "MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays" pptx
Ngày tải lên :
09/08/2014, 20:20
... (left) and the robustness of predictions in the face of increasing noise levels (right) these unsuccessful candidates early and at low cost Conversely, careful selection and optimization of candidate ... and candidate biomarkers (Additional data file 1); the XML-based PMML model that MethMarker uses for exporting, importing and storing candidate biomarkers (Additional data file 2) importing and ... 13 CB initiated the project and conceptualized workflow and software PS designed and implemented MethMarker, developed the case study tutorial, set up the website and drafted the paper TM devised...
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báo cáo khoa học: " Identification, utilisation and mapping of novel transcriptome-based markers from blackcurrant (Ribes nigrum)" pdf
Ngày tải lên :
11/08/2014, 11:21
... both SNP and SSR markers identified in this study Total RNA extraction Total RNA was extracted from 100 mg of frozen pooled developing bud material using the Plant RNeasy Mini Young leaf material ... from developing fruit and buds, and Sanger-sequenced several thousand ESTs [13,14] From these libraries, forty-three SSR and sixteen SNP markers have been mapped genetically and, together with AFLPs, ... design and molecular biology, and facilitated the 2GS procedures MB and LC provided bioinformatics support for the 2GS data CH analysed the mapping data CB and JAM provided sequencing and genotyping...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
Ngày tải lên :
12/08/2014, 03:20
... accessions, prima- Plant material and RNA extraction Leaves of globe artichoke, and cultivated cardoon were collected from experimental fields in Scalenghe, Torino (Italy) Total RNA was extracted from ... of globe artichoke and cardoon Reverse transcription from both globe artichoke and cardoon total RNA was achieved using poly(dT)primer and M-MuLV RNaseH-RT (Finnzymes, Finland), following the ... contributions SL and FB planned and supervised the work CC, AM, BM and AH carried out the molecular genetic studies; EP carried out phylogenetic and linkage analyses All authors read and approved...
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Báo cáo y học: "Value and price of ventilator-associated pneumonia surveillance as a quality indicator" potx
Ngày tải lên :
13/08/2014, 20:21
... interdisciplinary team Crit Care Med 2006, 34:211-218 Horan TC, Andrus M, Dudeck MA: CDC/NHSN surveillance definition of health care-associated infection and criteria for specific types of infections in the ... Intensive Care Med 2004, 30:996-997 doi:10.1186/cc8189 Cite this article as: Aardema LM, et al.: Value and price of ventilator-associated pneumonia surveillance as a quality indicator Critical Care 2010,...
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Báo cáo y học: "Strategy for encoding and comparison of gene expression signatures" pps
Ngày tải lên :
14/08/2014, 07:22
... hit ratio, and this is used to rank the confidence levels of data set matches reports Where N and M are numbers of concordant and discordant matches, respectively, and Siq and Sis (Sjq and Sjs ) ... from a query dataset and signatures in SigDB (see Materials and methods, below) The significance of the similarity was determined by a simulation analysis (see Materials and methods, below) Finally, ... include those reported by Staunton and coworkers [13] (dataset A), Butte and colleagues [12] (dataset B), Ross and coworkers [14] (dataset C), and GDS89 Datasets A and B were generated using Affymetrix...
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Báo cáo y học: "Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish" pdf
Ngày tải lên :
14/08/2014, 08:20
... STAT4 and STAT1 genes on human chromosome and Danio rerio chromosomes 22 and Synteny between regions containing STAT4 and STAT1 genes on human chromosome and Danio rerio chromosomes 22 and Genes ... genes we searched the zebrafish genome and all available zebrafish ESTs for the subdomains SD100A and SD100B (see Materials and methods, below) We identified 22 candidates, of which seven had incomplete ... nucleotidebinding domain/NACHT domain and leucine rich repeat containing family Materials and methods Software Standard web-based programs were used for sequence comparisons, alignments, and phylogenies The phylogenetic...
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Báo cáo y học: " Validation and extension of an empirical Bayes method for SNP calling on Affymetrix microarrays" pot
Ngày tải lên :
14/08/2014, 08:21
... Hubbell and Chuck Sugnet for helpful discussions; and finally, Ben Bolstad, James MacDonald, Seth Falcon, Marvin Newhouse, Robert Gentleman, Jiong Yang, and Fernando Pineda for help with hardware and ... HapMap and Affymetrix and, thus, these SNPs were corrected by hand For the replicate data, high quality chips (operationally defined as having an SNR greater than 4.5 and 2.36 for the 100K and 500K ... (Nsp and Sty) from the Broad Institute and Affymetrix, respectively Again, the gold standard is HapMap calls (c) ADPs are plotted for the 32 and 16 high quality replicates hybridized on XBA and...
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Báo cáo y học: " Advances in the genetics and epigenetics of gene regulation and human disease" pps
Ngày tải lên :
14/08/2014, 17:22
... of enhancers in c-Myc and N-Myc, genes that play a role in growth control and tumorigenesis From the same group, Mikael Björklund presented a genome-wide RNA interference (RNAi) analysis of genes ... maternal nutrition For example, supplementary nutrition can lead to increasing body weight across several generations of offspring He and his colleagues postulate that maternal nutrition before and ... types and pathological conditions Christoph Bock (Max Planck Institute for Informatics, Saarbrücken, Germany) described his group’s work predicting CpG methylation on the basis of DNA sequence and...
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Accelerating Test, Validation and Debug of High Speed Serial Interfaces
Ngày tải lên :
09/04/2015, 17:22
... where standard instruments, such as oscilloscopes, signal generators and logic analyzers are used The standard measurement equipment is also referred to as bench equipment, and the validation and ... transmitter power, the timing jitter, and the method of demodulation and decoding In baseband transmission, the data and clock are combined on the transmitter side and transmitted as digital waveforms ... Verma, Bill Burchanowski, and Sandeep Kumar for collaborating on a work that led to co-authoring one paper We also appreciate the technical support and help from the whole PHY and Storage team at Agere/LSI,...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
Ngày tải lên :
16/02/2014, 15:20
... cytoplasmic Rluc mRNA accumulation was measured and, to normalize the transfection and recovery efficiencies, the accumulation of Fluc mRNA was measured in the same RNA samples (Fig 7D) CP and TTP-CP ... associated Materials and methods Materials Enzymes were purchased from Invitrogen (Carlsbad, CA, USA) and Roche (Basel, Switzerland); oligonucleotides were obtained from Sigma (St Louis, MO, USA); and ... ribonucleoprotein hnRNP M, the RNA helicases RHII ⁄ Gu ⁄ DDX21 and p68 ⁄ DDX5, and the SR protein ASF ⁄ SF2 (Fig 1C) The interactions of TIAR with hnRNP M, DDX21 and DDX5 helicases and ASF ⁄ SF2 were further...
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Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebrafish (Danio rerio) potx
Ngày tải lên :
31/03/2014, 07:20
... B-FABP mRNA expression RT-PCR was used to determine the spatial and temporal distribution of B-FABP mRNA in adult and embryonic zebrafish Total RNA was extracted from adult zebrafish tissues and embryos ... between zebrafish and human, mouse and pufferfish B-FABP is 83%, 76% and 83%, respectively The percentage amino acid identity between zebrafish and human and mouse is higher in the exons and than it ... zebrafish B-FABP and b-actin mRNAs in adult tissues The histogram shows the ratio of B-FABP mRNA to b-actin mRNA in various tissues with abundant expression of the B-FABP mRNA seen in RNA extracted...
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Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio) pot
Ngày tải lên :
31/03/2014, 07:20
... fabp3 genes from mammals and zebrafish were a result of evolutional divergence of transcriptional regulation Materials and methods Growth and maintenance of zebrafish adults and embryos Zebrafish were ... gel-electrophoresis Three bands of 250 bp, 200 bp and 180 bp in the TAP+ reaction were excised from the gel and purified using the Qiaquick gel extraction kit (Qiagen) The two minor bands ( 250 bp and 180 ... The minor band (Fig 2) was reamplified by PCR and repurified The purified DNA fragments were cloned into the plasmid vector, pGEM-T (Promega, Madison, WI, USA), and three of the major band and one...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
Ngày tải lên :
20/06/2014, 01:20
... of SOCS3, ISG15 and IFNβ mRNA expression Results RSV stimulation of SOCS1, SOCS3, IFNα and IFNβ mRNA expression To determine the relationship between RSV infection, RSV proteins, and SOCS regulation ... apoptosis linked to infection [3] RSV and RSV mutant virus infection of MLE-15 cells at 24 h pi was associated with IFNα, IFNβ and SOCS1 and SOCS3 mRNA expression SOCS1 mRNA expression was significantly ... SOCS3 mRNA expression, an effect associated with NS1/NS2 expression Despite differences in SOCS1 mRNA expression, the levels of IFNα and IFNβ mRNA expression were similar between RSV and RSV...
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