material and structural basis of skeletal fragility
Báo cáo khoa học: Natural and amyloid self-assembly of S100 proteins: structural basis of functional diversity doc
Ngày tải lên :
23/03/2014, 03:20
... the EF-hands in S100 proteins Structure of the N-terminal, S100-specific EF-hand (A) and the C-terminal, canonical EF-hand (B) in the metal-free (lighter) and Ca2+-bound (darker) form of S100A6 ... deposition and tissue remodelling [14] The conversion of functional proteins and peptides into insoluble amyloid structures and their deposition in a variety of tissues and organs is a hallmark of a ... ultimately bring us to a higher level of understanding the biology of tumour and degeneration and enable to use our acquired knowledge of S100 structure and functions in developing strategies...
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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
Ngày tải lên :
14/02/2014, 22:20
... studies of the structure and binding sites of enzymes Inhibitor binding to ribonucleases J Mol Biol 45, 491511 33 Ui N (1971) Isoelectric points and conformation of proteins I Effect of urea ... Fc| RhFo, where Fo and Fc are the observed and calculated structure factor amplitudes of reection h, respectively Rfree is equal to Rcryst for a randomly selected 5.0% subset of reections not used ... structural interactions of a nucleic acid with a protein Conclusions The functional and structural studies presented herein demonstrate the attributes of N-acylsulfonamidyl and sulfonimidyl groups...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
Ngày tải lên :
18/02/2014, 17:20
... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, a proregion of ... bonds for Asn11(CO)–Trp14(HN) and Ser12(CO)– Thr15(HN), and confirmed by the slow solvent exchange kinetics of the amide protons of Trp14 and Thr15 The observation of two small 3JHN-Ha coupling...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
Ngày tải lên :
22/02/2014, 07:20
... the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most of the residues are in the allowed regions of low energy, except ... respectively, fixed at 4.0 and 80.0, and the calculations were performed keeping a salt concentration of 0.14 M NaCl No even distribution of the net negative charge of the carboxylic group of negatively charged ... SDS/PAGE yielded a single polypeptide band of < 55 kDa (Fig 1A) The unreduced protein also yielded a major band of 55 kDa but exhibited an additional minor band of slightly higher molecular mass (
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
Ngày tải lên :
07/03/2014, 12:20
... BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic treatment ... using a combination of CM-TSK and RP-HPLC The final material was homogenous, as shown by the presence of a single band on SDS ⁄ PAGE, a single sharp peak on RP-HPLC, and a single band on immunoblotting ... Fig cDNA sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
Ngày tải lên :
07/03/2014, 15:20
... component bands, determined by band fitting of the absorbance spectrum of Fig 4A, are shown in Fig and Table From Table 2, the sum of individual amide I¢ intensity and the intensity percent of each ... the presence of a very strong negative band at 198 nm The negative band near 220 nm results from overlapping of the bands of b-sheet (215 nm) and a-helix Table Fractional composition of secondary ... purification, identification and characterization of a member of the PKI family By cloning and construction of a high- expression Ó FEBS 2004 Purification and structural study of human PKIb (Eur J Biochem...
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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deficient families from Southern Italy docx
Ngày tải lên :
16/03/2014, 01:20
... to Erlandsen and Stevens [22] The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, ... means that it is often difcult to predict the phenotype on the basis of a patients genotype, and further studies in different ethnic groups are still warranted Function and structure of PAH human ... family and a shorter fragment of 253 bp present only in the proband, as well as in his mother who bears the same mutation (Fig 3) Direct sequencing of both cDNA bands conrmed the skipping of the...
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Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf
Ngày tải lên :
16/03/2014, 16:20
... mutant, and Fe-type NHase are shown in yellow, red and cyan, respectively The Cc2 atom of aThr109 and the Cc1 atom of aVal136 of the Co-type NHase are connected by a yellow line (B) Vicinity of aTyr114 ... mutated residue in the aT109S and aY114T structures, and a cobalt atom and three oxygen atoms in the apoenzyme and aY114T structures Several rounds of refinement and model Ó FEBS 2003 432 A Miyanaga ... BSA as the standard The cobalt and iron contents were determined by ICP-AES (SPS-1200 V; Seiko Instruments, Chiba, Japan) using sample solutions, and standard solutions of cobalt and iron (Wako...
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Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx
Ngày tải lên :
16/03/2014, 18:20
... influence of standard variables (pH of the solution, reaction time, and binding Table Influence of the reaction time (t) and binding ability of the counter-ions (X) on the nature and structural ... Notwithstanding this, there is a minor effect of t and X at the beginning and end of the titration Thus, the addition of the first 4–6 of Hg(II) to apo-MT gives rise to Hg-MT species of comparable ... system required the performance and analysis of 18 Ó FEBS 2004 titrations and the corresponding duplicates The detailed and comparative analysis of the set of CD, UV-vis and difference electronic absorption...
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Báo cáo khoa học: Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom Identification of the origin of a new viper population based on ammodytin I1 heterogeneity docx
Ngày tải lên :
17/03/2014, 03:20
... ammodytins I1 and I2 and ammodytin L genes of V am ammodytes, the VP7 and VP8 genes of V palaestinae, and the PLA2 genes of Crotalinae snakes (Trimeresurus flavoviridis) were also of similar length ... Analysis of venom gland PLA2 cDNAs is currently underway to determine which of these genes are expressed The presence in the neurotoxic snake of two ammodytin I1 isoforms (In and Ia) and of several ... the sequences of V a zinnikeri and neurotoxic V a aspis (DDBJ/EMBL/GenBank accession no.s AY152843 and AF548351) and that of vaspin chain B, from the sequences of V a zinnikeri and neurotoxic...
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Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx
Ngày tải lên :
17/03/2014, 17:20
... (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein Results and Discussion Annotation of CMs in M tuberculosis ... 90-MtCM and 105-MtCM at chorismate concentrations of 100 lm to mm Both MtCMs catalyzed the conversion of chorismate to prephenate with a kcat of 5.5 ± 0.2 s)1 and a Km of 1500 ± 100 lm at 37 °C and ... shorter version of helix of EcCM, TtCM and PfCM lacking two of the binding site residues (Fig 4C) corresponding to Ser84 and Gln88 of EcCM as discussed below During the preparation of this article,...
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hydrothermal synthesis and structural characterization of fe2o3sno2 nanoparticles
Ngày tải lên :
19/03/2014, 16:48
... evidence of the solubility limits of Sn4þ in the hematite structure and of Fe3þ in SnO2 are discussed the compositional uniformity The determined average values of x; for the analyzed series of mixed ... with coordinates of ð0; 0; zÞ occupy 2/3 of the octahedral holes in successive oxygen layers, and 1/3 of the octahedral holes with coordinates of ð0; 0; 0Þ are empty In the case of our samples, ... shape and the disappearance of magnetic hyperfine structure as tin content in the samples M Sorescu et al / Journal of Physics and Chemistry of Solids 65 (2004) 1021–1029 Fig TEM image (a) and the...
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Báo cáo khoa học: Structural basis of p63a SAM domain mutants involved in AEC syndrome ppt
Ngày tải lên :
22/03/2014, 16:20
... ideal geometry for bond lengths and restraint angles (Engh and Huber) g Percentage of residues in the ‘most favoured region’ of the Ramachandran plot and percentage of outliers (PROCHECK) h Protein ... contribute to an understanding of the potential destabilizing or functional effects of AEC mutations within the p63a SAM domain Materials and methods Cloning, expression and purification of the p63a SAM ... ⁄ 15N assignments and structure determination were carried out using standard methods as described in the Materials and methods A summary of all conformational constraints and statistics is presented...
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Báo cáo khoa học: X-ray crystallography and structural stability of digestive lysozyme from cow stomach doc
Ngày tải lên :
23/03/2014, 04:21
... Structure and stability of bovine stomach lysozyme Y Nonaka et al Table Comparison of structural parameters among lysozymes BSL2 No of residues No of charged residues Negative Positive No of salt ... BSL2 and HEWL is ˚ , and that between BSL2 and house fly lysozyme 0.35 A ˚ is 0.79 A The backbone structure of BSL2 is closer to that of the vertebrate nondigestive lysozyme than to that of insect ... b-domain is composed of a large loop and a three-strand antiparallel b-sheet Figure 1B is a superimposition of the main chain conformations of BSL2, human lysozyme, HEWL, and house fly midgut A...
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Báo cáo khoa học: Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima ppt
Ngày tải lên :
23/03/2014, 09:20
... of six a-helixes and has one central b-sheet made up of six b-strand-strands (Fig 3A) The first and second b-strand of the a ⁄ bhydrolase fold have not been modeled The quality of the model towards ... homologues and sequences of the esterase and lipase families using the Tcoffee server (http://www.igs.cnrs-mrs.fr/Tcoffee/tcoffee_cgi/index cgi) [36] The alignment was further corrected by hand A ... both sides of Ser243 The alcohol side of the substrate is in a groove pointed towards the entrance ˚ of the pocket and extends approximately 10 A from Ser243 The acyl-side of the ligand fits in...
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Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx
Ngày tải lên :
23/03/2014, 09:21
... responsible for the different substrate specificity of hPNP and hMTAP (Glu201, Asn243 and Val245 of hPNP instead of Ser178, Asp220 and Asp222 of hMTAP, respectively) The last important substitution ... and the elution was carried out with : 95 (v ⁄ v) mixture of 95% methanol and 0.1% trifluoroacetic acid in H2O The retention times of inosine and hypoxantine, guanosine and guanine were 10.5 and ... function of time and protein concentration One unit of enzyme activity was defined as the amount of enzyme that catalyzes the cleavage of lmol of inosine per minute at 80 °C Determination of kinetic...
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Báo cáo khoa học: The structural basis of calpain behavior pptx
Ngày tải lên :
23/03/2014, 10:21
... and oscillations of [Ca2+], in particular those involving specific phosphorylations of calpastatin, calpains and targets The following Minireviews develop three themes on the role of calpain and ... The structural basis of calpain behavior Y Benyamin two parts, DIIa and DIIb, come closer together, with concomitant release of constraints between the domains ... Calpains and disease N Engl J Med 352, 2413–2423 Hosfield CM, Elce JS, Davies PL & Jia Z (1999) Crystal structure of calpain reveals the structural basis for Ca2+dependent protease activity and a...
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Báo cáo khoa học: Synthesis and structural characterization of a mimetic membrane-anchored prion protein doc
Ngày tải lên :
23/03/2014, 10:21
... composed of 35% a helix and a small amount of b sheet, whereas PrPSc appears to have elevated levels of b sheet [7,8] Higher resolution studies of the structure of PrPC have made use of NMR and ... thorough understanding of the folding and interactions of PrP in its tethered conformation on the plasma membrane The interaction of PrP with different lipid components is complex and is not completely ... 220 s and uncoupled material elutes at around 180 s (B) Electrospray MS and deconvoluted MS (inset) of PrP–GPIm The measured mass of 24 064.3 Da agrees with the expected calculated mass of 24...
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Báo cáo khoa học: Correlation between functional and structural changes of reduced and oxidized trout hemoglobins I and IV at different pHs doc
Ngày tải lên :
23/03/2014, 12:20
... HbI and HbIV: iron(II)-HbI and met-HbI induce a 23.2 and 50.1% reduction of the peak intensity, respectively, when compared with the peak of the buffer Iron(II)-HbIV and metHbIV induce a 35.8 and ... [5,6] The mechanism of hydrogen peroxide removal by iron(II)-Hb (oxyHb and deoxyHb) and met-Hb results in the formation of ferrylhemoglobin (ferrylHb) and oxoferrylhemoglobin (oxoferrylHb), respectively ... explained by the reaction of oxoferrylHb with H2O2 with the production of met-Hb and oxygen instead of superoxide anion [8] Our data performed, at pH 5.4, in the presence of H2O2 and guaiacol (as reductant)...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
Ngày tải lên :
23/03/2014, 13:20
... GELGIYLVGDDSLFPWR and IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band ... (1D, the horizontal bands) and then in nondenaturing conditions by BN ⁄ PAGE (2D) The molecular mass standards and direction of migration are indicated for both the first (1D) and second (2D) dimension ... types of plastids The Ndh complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS...
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