master plate preparation and distribution for high throughput screening

... [9], and cancer [10-12] Important ligands for CCR5 are the βchemokines 'regulated on activation normal T cell expressed and secreted' (RANTES), and the 'macrophage inflammatory proteins' MIP-1α and ... cells and the MOLT4.CCR5, Jurkat.CCR5 and SupT1.CCR5 cells were washed and resuspended at × 104 cells/ml in medium and seeded out in 24 well plates HIV-1 laboratory strains BaL, NL4.3 and HE ... [13] and in human macrophages [14] Unlike CCR5, the CXCR4 receptor has only one known ligand, the α-chemokine 'stromal cell derived factor' (SDF)-1 Since the natural CCR5 and CXCR4 ligands and...

Ngày tải lên: 13/08/2014, 13:20

Ngày tải lên: 21/02/2014, 05:20

... produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for the production and selective ... scrambling and easier sample handling compared to in vivo protein production render cell-free protein synthesis particularly attractive for high- throughput production of proteins and selective ... interconversion between Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20]...

Ngày tải lên: 07/03/2014, 12:20

... genomes (119 replicons) The ISsaga platform has been designed to maintain common standards for high quality IS annotation used in ISfinder at both protein and nucleotide levels It is a web-based ... intercalated or tandem ISs; and IS-driven flanking gene expression (for example, formation of hybrid promoters) [3] The ability to identify partial IS copies, intercalated ISs and IS derivatives, ... combination for providing high quality data, especially when subject to expert human inspection and validation The numerical importance of transposases in nature [4], and presumably, therefore, the...

Ngày tải lên: 09/08/2014, 22:24

... Engl 44, 1048-1053 (2005) Hu, Y., Uttamchandani, M & Yao, S.Q Microarray: a versatile platform for highthroughput functional proteomics Comb Chem High Throughput Screen 9, 203-212 (2006) x List ... from three different aspects for high- throughput identification of enzymes and their associated proteins in yeast proteome (catalomics) Firstly, to validate the high- throughput capacity of differential ... protein biotinylation strategies for potential high- throughput proteomics J Am Chem Soc 126, 10551062 (2004) Hu, Y., Chen, G.Y.J & Yao, S.Q Activity-based high throughput screening of enzymes using...

Ngày tải lên: 16/09/2015, 08:30

... images and EDS of SiOx nanowires deposited on the Si(111) substrate at the reaction temperature of 1000 °C for h (a) and (b), The lower magnification SEM images of the nanowires, (c) The higher ... reaction temperature of 1000 °C for h Large scale, high density nanowires are uniformly covered on the surface of Si(111) substrate, as illustrated in Fig 2(a) and (b) The lengths of these nanowires ... catalysts play a key role in the formation of SiOx nanowires, and a VLS mechanism is the most probable growth mechanism Here, we give a description for the possible formation of SiOx nanowires Firstly,...

Ngày tải lên: 16/03/2014, 15:11

... diameter and about 5–10 nm in particles diameter, Fig 6(a–f)) The SEM, TEM, and SAED images of the nanosheet TiO2 calcined at 600 and 700 °C showed almost nanoparticles with a mixture of anatase and ... surface is 642 m2/g), and the pore size distribution of the sample with pore diameter about 3–4 nm (inset) method at 130 °C for 12 h The nanosheet structure was slightly curved and approximately ... nanosheet TiO2 and nanosheet TiO2 calcined for h at 300–700 °C 2976 S Pavasupree et al / Materials Letters 61 (2007) 2973–2977 Fig SEM, TEM, and SAED images of the nanosheet TiO2 calcined for h at...

Ngày tải lên: 20/03/2014, 13:06

... Radiation threats and your safety A guide to Preparation and REsponse for Professionals and Community Radiation threats and your safety A guide to Preparation and REsponse for Professionals and Community ... numerous guidance and planning documents, and some of that information keeps changing as new plans are drawn and new terminologies and acronyms are created Also, numerous fact sheets and information ... of radiation and radioactivity, at least for many, is formed by images of mushroom clouds, cinema and television dramatizations, and even cartoon characters The need for understanding radiation...

Ngày tải lên: 30/03/2014, 02:20

... 0.59 for plate 1, 0.42 for plate 2, and 0.10 for plate A complete analysis of performance is shown in Table Table Statistical Analysis of Data from the Three Plates Described in Fig Parameter Plate ... High Throughput Screening Assays 1 Design and Implementation of High Throughput Screening Assays Ricardo Macarrón and Robert P Hertzberg Introduction In most pharmaceutical and biotechnology ... FLIPR: a new instrument for accurate, high throughput optical screening J Biomol Screen 1, 75 – Hertzberg, R P., and Pope, A J (2000) High throughput screening: new technology for the 21st century...

Ngày tải lên: 11/04/2014, 09:46

... al Fig 2.1 High- throughput sequencing workflow There are three main steps in high- throughput sequencing: preparation, immobilization, and sequencing Preparation of the sample for highthroughput ... Bioinformatics for High Throughput Sequencing Naiara Rodríguez-Ezpeleta Ana M Aransay ● Michael Hackenberg Editors Bioinformatics for High Throughput Sequencing Editors ... molecular biology and biochemistry The ultra -high- throughput instruments are essentially high- tech machines and understanding the engineering principles gives the user the ability to command and troubleshoot...

Ngày tải lên: 29/05/2014, 14:33

... valid for a class of models and robust against uncertainties in specific models positive X ∗ and F (X ∗ ) We write • avoids expensive numerical approximation of steady states and can be scaled to highdimensional ... author Abstract Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes ... intuitive, e.g the aX Holling type-II functional response G(X) = k+X is linear for low density X (gX ≈ 1) and saturates for high density X (gX ≈ 0) So far we succeeded in expressing the Jacobian of...

Ngày tải lên: 18/06/2014, 18:20

... valid for a class of models and robust against uncertainties in specific models positive X ∗ and F (X ∗ ) We write • avoids expensive numerical approximation of steady states and can be scaled to highdimensional ... author Abstract Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes ... intuitive, e.g the aX Holling type-II functional response G(X) = k+X is linear for low density X (gX ≈ 1) and saturates for high density X (gX ≈ 0) So far we succeeded in expressing the Jacobian of...

Ngày tải lên: 20/06/2014, 01:20

... valid for a class of models and robust against uncertainties in specific models positive X ∗ and F (X ∗ ) We write • avoids expensive numerical approximation of steady states and can be scaled to highdimensional ... author Abstract Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes ... intuitive, e.g the aX Holling type-II functional response G(X) = k+X is linear for low density X (gX ≈ 1) and saturates for high density X (gX ≈ 0) So far we succeeded in expressing the Jacobian of...

Ngày tải lên: 20/06/2014, 21:20

... coexistence performance assessment and techniques for WiMAX services from highaltitude platform and terrestrial deployments,” by Z Yang et al., the authors investigate the performance and coexistence ... served by a multi-antenna payload, and both uplink and downlink performances are analyzed The forth paper “Coexistence performance of highaltitude platform and terrestrial system using gigabit ... coexistence techniques for worldwide interoperability for microwave access (WiMAX) delivered from high- altitude platforms (HAPs) and terrestrial systems in shared 3.5 GHz frequency bands The paper shows...

Ngày tải lên: 21/06/2014, 23:20

... with a MasterScan database MFQL queries are search masks for probing lipid spectra for the features stored in the MasterScan, such as precursors and fragment masses and their compositional and abundance ... of employed tandem mass spectrometer LipidXplorer imports spectra in generic mzXML format and converters from proprietary formats to mzXML are available for major instrument platforms Here we ... and speeds up lipid identification in high- throughput screens, improves ion statistics and limits the rate of false positive assignments To the best of our knowledge, comparable flexibility and...

Ngày tải lên: 09/08/2014, 22:23

... 65°C for or 80°C for min, respectively After denaturation, candidate aptamer and target were quickly cooled down to 4° C for and then equilibrated at room temperature (RT) for before adding the ... the TOPO TA cloning kit (Invitrogen) Synthesis, capture and sequencing of the candidates In order to generate candidates for high throughput screening, we set up a second automated workstation (Tecan ... the target and A beads to the candidate to be tested This is repeated for a number of candidates issued from a given SELEX round and the emission specific to each pair is measured Candidates generating...

Ngày tải lên: 11/08/2014, 00:23

... susceptibility index Manual screening has been done for a large number of experiments and this proved to be a tedious and very time-consuming mission The desired screening of thousands of candidate genes ... the data set and used one half for training the classifier and the other half for testing To obtain a stable informational value, this partitioning, training, and testing was performed in terms ... incubated for another 40 h, followed by staining of transformed cells for beta-glucuronidase (GUS) activity and microscopy Cell segmentation Due to the performed staining (cf Figures and 4), hue...

Ngày tải lên: 12/08/2014, 05:20

... signatures of interest with great rapidity and flexibility, and at very high throughput and dramatically lower cost, represents, in our view, the next transformative technology in the gene expression ... coated 384 well plates (GenePlateHT; RNAture) from total RNA (500 ng) in Lysis Buffer (RNAture) or whole cell lysates (20 μl) Plates were covered and centrifuged at 500 g for one minute, and incubated ... in 1× Taq Ligase Buffer (New England Biolabs, Ipswich, Ma, USA; μl), the plate covered, spun as before, heated at 95°C for two minutes and maintained at 50°C for six hours Unannealed probes were...

Ngày tải lên: 14/08/2014, 16:21

... Suitable for psychological, physical characteristics and development of students; 2) Close to the program for higher secondary education; 3) Practical, close to life and production; 4) Suitable for ... evaluation plan and design evaluation toolkit for project method of teaching in production and use of electric power The toolkit has dual purpose (qualitative and quantitative evaluations) and will ... production and use - higher secondary Physics (54 pages, including figures and tables) Chapter Designing process of teaching by project method in knowledge of electric power production and use – higher...

Ngày tải lên: 17/08/2015, 21:21