massarray™ for confirmation and validation of single nucleotide polymorphisms

... favourably to these other performance metrics In the single nucleotide polymorphism database (dbSNP) of the National Center for Biotechnology Information (NCBI), more than million polymorphisms including ... 83 83 83 83 287 287 287 287 10 Figure Nucleotide sequence alignment of amplicon sequences of a probe set Nucleotide sequence alignment of amplicon sequences of a probe set Polymorphic residues ... microarrays for transcriptome analyses in a range of rice RILs, NILs and germplasm accessions, existing data files provide abundant opportunities for the identification of additional SFP markers and...

Ngày tải lên: 12/08/2014, 03:20

... the association of GST polymorphisms and cancer outcome (mainly colorectal, breast, and lung malignancy) SNPs for GST polymorphisms were used as a substrate for analyses Materials and Methods Search ... difference for any of the GST polymorphisms for at least one of the following outcome measures: overall survival or disease recurrence or response to treatment In the case of lack of a clear ... authors and the competition for acceptance in journals are fierce [3,4] and may lead the authors to perform studies with few polymorphisms, which need less time to complete, and to submit for publication...

Ngày tải lên: 25/10/2012, 10:51

... ethnical committees of the Medical School of Nanjing University, and single nucleotide Page of (page number not for citation purposes) polymorphism (SNP) Research Center of RIKEN, and informed consent ... 0.28 and P = 0.27 in recessive and allele modes, respectively) We had 99% and 63% power to detect odds ratios of 2.1 and 1.5 in our study TXNDC3 Frequencies of the susceptible T allele of rs4720262 ... samples and participated in the design and analysis of the study, and MN genotyped the Japanese samples JD, JQ, NJ, YX, CY and JW evaluated the patients and genotyped Chinese samples, and TN helped...

Ngày tải lên: 09/08/2014, 10:23

... thank Dr Andrea Puebla and and Pablo Vera for gene sequencing and Natalia Almasia and Florencia del Viso for critical reading of the manuscript This study is conducted within the frame of the Sunflower ... complete set of 28 genes studied in Table b Nucleotide polymorphism and nucleotide diversity obtained by Liu and Burke [46] inbred lines, 0.0078 for the P&I cultivated group and 0.0079 for the pooled ... the resulting nucleotide diversity and LD estimates to those previously reported for wild and cultivated sunflower Results SNPs frequency and nucleotide diversity A total of 64 candidate regions...

Ngày tải lên: 12/08/2014, 05:20

... dna %19.04 dewohs reed akis citsemod naeroK eht ,PNS 676 eht fo elella G eht rof %55 dna PNS 804 eht fo elella C eht rof %55 dah reed akis citsemod esenihC eht elihW ]6[ reed akis citsemod esenihC ... esenihC ni deifitnedi neeb evah PNRP reed akis eht ni sPNS ehT )1 elbaT( ylevitcepser ,G dna C rof %25.15 dna 84.84 erew msihpromylop taht fo seicneuqerf elella ehT )4 giF( dica cimatulg ot enicylg ... 622 nodoc ta detacol )676 editoelcun ta G → C( PNS eht ,revewoH )1 elbaT( ylevitcepser ,C dna T rof %19.04 dna 90.95 erew msihpromylop taht fo seicneuqerf elella ehT ]6,5[ napaJ dna anihC gnitibahni...

Ngày tải lên: 07/08/2014, 23:22

... 'Fair', and 'Poor') Items that significantly predicted this variable were candidates for inclusion in the utility exercise Page of (page number not for citation purposes) Health and Quality of Life ... is the mean TTO value for each health state and x is a vector of dummy explanatory variables (x∂λ) for each level λ of dimension ∂ of the simplified CAMPHOR classification For example, x31 denotes ... than 0.05 (35% for the mean level model and 38% for the random effects models, respectively) and both models resulting in two predictive errors greater than 0.10 In both mean and random effects...

Ngày tải lên: 18/06/2014, 19:20

... were 0.424 for disease and physical; 0.515 for physical and social; 0.777 for social and psychological; and 0.612 for psychological and satisfaction Criterion validity of the short-form of BPH-QLS ... domains of the short-form of BPH-QLS and SF-36 were significantly correlated (p < 0.01) with CCs of 0.267~0.773, most of which were > 0.300 The disease domain of the new scale and that of the ... between shortform of BPH-QLS and BPH-QLS with CCs of 0.864– 0.980 (table 3), and correlation of the total score between the two scales r2 = 0.947 The short-form scale accounted for 94.7% of the explained...

Ngày tải lên: 18/06/2014, 19:20

... included an information sheet and a consent form as well as a new questionnaire and a short demographic form At the time of recruitment for this project, the Volunteer Database consisted of 197 carers ... The aim of the carer workshops was to provide information and skills training for carers of people with eating disorders in order to improve their coping strategies and reduce the levels of difficulties ... and qualitative work with carers and reviewed by a panel of "expert carers" [12,16,26,27] The panel was made up of a psychiatrist (one of the authors of this paper), a clinical nurse leader and...

Ngày tải lên: 18/06/2014, 22:20

... 59 and 75 for depression, and 61 and 61 Lastly, we computed means for men and women and each subscale as a function of cancer type Only those types of cancer were listed where at least men and ... anxiety and depression Bjelland et al [23] provided a review of the psychometrics of HADS based on 747 published studies and reported Cronbach's alphas of 68 to 93 for anxiety and 67 to 90 for depression ... of Health and Human Services, Centers for Disease Control and Prevention Andresen E, Catlin T, Wyrwich K: Retest reliability and validity of a surveillance measure of health-related quality of...

Ngày tải lên: 20/06/2014, 15:20

... achieved for dry eye symptoms and these symptom sub-concepts, as well as for all of the overarching concepts of dry eye daily life impact and for the aforementioned sub-concepts Concepts and sub-concepts ... between some of the IDEEL dimensions and the SF-36 (such as “impact on work” of IDEEL and “role physical” of SF-36; “emotional impact” of IDEEL and the mental component (or some of the related ... process of development and finalisation of the questionnaire They gave their approval at each of the milestones of the development and finalisation process The questionnaire was also reviewed and...

Ngày tải lên: 20/06/2014, 15:20

... information of the environment is limited, and due to more frequent unpredictable phenomenas such as moving vehicles and fast fading Scenario and Measurements The scenario for the evaluation of ... out of scope of this paper [22] EURASIP Journal on Wireless Communications and Networking MR-FDPF model is the second component of the combined approach of this paper 2.3 Comparison RO models and ... calibration of the parameters of the model in order to make it suitable for indoor simulation even if the model is restricted to 2D [21]; (iii) an improvement of the model in order to perform OFDM simulations...

Ngày tải lên: 21/06/2014, 11:20

... sensitivity) and false-positive ratios (or 1-specificity) of a particular cut-off value, and may help in selecting the optimal cut-off value for a new scale: i.e assuming an optimal FAS cut-off value of ... mean (standard deviation) of FAS was 6.34 (1.61) and the 95% CI of the mean was 6.09 to 6.49 Validity analysis The construct validity of the FAS index was examined in terms of convergence and discriminant ... Ethics Committees of the Polytechnic University of the Marche Medical School, and the Sacco University Hospital, and all of the patients gave their informed consent Identification of important efficacy...

Ngày tải lên: 09/08/2014, 14:22

... monitoring and intervention for these disorders From an ethical perspective, there may be a growing responsibility for physicians to offer these forms of testing For carrier testing for recessive ... availability of carrier testing and prenatal diagnosis for some forms of deafness could lead to an obligation to inform couples of this [18] Perhaps it is reassuring that, to my knowledge, couples and ... Prader-Willi and Angelman syndromes and in identifying candidate gene regions for disease in children born of first cousin and similar matings However, the occurrence of incest, as in the mating of a...

Ngày tải lên: 11/08/2014, 12:20

... Discussion The sensitivity and specificity of the Korean CAM-ICU was 89.8% and 77.4%, respectively, for nurse and 72.4% and 75.8% for nurse Overall agreement () between nurse and nurse was 0.81 (p ... compared with the reference standard were 89.80% for nurse and 77.40% for nurse Their specificities were 72.40% and 75.80% and their overall accuracies were 83.33% and 88.37% respectively (Table ... Professor E Wesley Ely for his approval and research nurse specialists for their support in the ICU Delirium and Cognitive Impairment Study Group We are grateful to the doctors and nurses of...

Ngày tải lên: 11/08/2014, 15:22

... measurements of both number median diameter and geometric standard deviation (GSD) are shown The GSD is a measure of dispersion for a log-normal distribution and is analogous to the standard deviation for ... the flow rates used for the NBIES for exposures) deliver a dry and uniform particle size to each of the NBIES' five ports Therefore, inhalation (and presumably lung deposition) of the generated ... those of humans, and have metabolic and physiologic similarities with humans They are an appropriate animal model [34] for study of the pathogenicity [20,35] and transmissibility [36,37] of influenza...

Ngày tải lên: 12/08/2014, 04:20

... grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to YN, KU-T, KS, and YT), the Ministry of Health, Labour and Welfare of Japan (to YT), and the Japan Health ... conformation and LDI (long distance interaction) conformation of the HIV-1 leader RNA [18] (see Additional file 4) It should be noted that the efficacy of si575 differed when targeting pNL4-3 and ... contributions YN performed the computational analyses and the target mRNA cleavage assays, participated in the design of the study, and drafted the manuscript KN and TO performed the viral replication...

Ngày tải lên: 13/08/2014, 05:22

... increased levels of total IgE, and increased levels of anti- Page of A fumigatus specific IgE, IgG and IgA antibody levels In the present study, we examined HLA class II antigens and IL-4RA polymorphisms ... washed and stained with murine monoclonal antibody to CD23-PE and CD20 Per-CP (Becton Dickinson) PBMCs were washed and fixed with 1% PBS buffered paraformaldehyde Forward and sidescatter was performed ... and allergic bronchopulmonary aspergillosis (ABPA) In our studies of ABPA, we identified risk factors for the development of ABPA: (1) HLA-DR2 and HLA-DR5 restriction [25,26] and (2) IL-4RA single...

Ngày tải lên: 13/08/2014, 13:22

... materials Three pairs of oligonucleotides were used in the SCAR assays [11], namely forward oligonucleotide SR-S343-F1 and the three reverse oligonucleotides SR-S343-R1, SRS343-R2 and SR-S343-R3 (Table ... SCAR reaction was performed with an initial denaturation step at 95°C for five minutes, followed by 35 cycles of 94°C for two minutes, 60°C for one minute, 72°C for one minute and a 10-minute final ... and Sn-S62-r indicated single nucleotide replacements of guanine to adenine and cytosine to guanine in B nivea var nivea and B nivea var tenacissima, respectively (Figure 3) Only a fraction of...

Ngày tải lên: 13/08/2014, 14:20

... times of ULOQ and diluted with blank plasma to get 1/5 and 1/10 concentrations of the spiked sample or as per requirement Calibration standards and six aliquots each of diluted samples (1/5 and ... 393.5/205.4 260.3/116.2 and and and and 10 (IS) 40 (IS) 25 (IS) (IS) Method validation 3.1 Specificity To verify the absence of interfering substances around the retention time of analytes, 20 blank ... Preparation of standard and sample solutions Standard stock solutions of naftopidil (1 mg/mL) and the IS (1 mg/mL) were separately prepared in 10 mL volumetric flasks with methanol Working solutions for...

Ngày tải lên: 02/09/2015, 13:48

... instantaneous gain and loss efficiencies by experimental data for climate conditions of Muscat, Oman He also compared similar operating and climate conditions with single slope solar still and also he ... vertical side of still was kept as 0.30 m, whereas the height of higher vertical side was kept 0.85 m The effective basin area of meter x meter made of GRP of m thickness Condensing cover made of toughed ... cover made of toughed glass of mm is fixed to the top of vertical walls of the stills using a rubber gasket on both sides of glass and clamp fixed iron frames made of angles A plastic pipe is...

Ngày tải lên: 05/09/2013, 15:28