mass spectrometric analysis of mucin core proteins

... cross-link products) Each mass in the combined mass list is matched within a custom defined mass window Computer-assisted mass spectrometric analysis of cross-links with the masses of the peptides from ... 283 Computer-assisted mass spectrometric analysis of cross-links L J de Koning et al Fig Part of the output sheet of the VIRTUALMSLAB analysis of the MALDI-FTICR-MS data of the RNase A digest ... with mass analysis, and by taking advance of the high mass accuracy of FTICR-MS In conclusion, it appears that advanced mass spectrometric studies on proteins can significantly be promoted by software...

Ngày tải lên: 07/03/2014, 12:20

... SDS/PAGE and Western blot analysis of pollen diffusates SDS/PAGE and Western blot analysis of pollen diffusates Approximately 10 µg of diffusate was loaded in each lane of a 12.5% SDS/PAGE and ... 6, 11 and 16) Proteomic analysis of pollen diffusates A proteomic approach, enabling rapid identification and analysis of proteins, was employed to confirm the identities of the bands surrounding ... The identities of other intensely staining bands were also examined to verify that mass spectrometry was suitable for analysis of pollen proteins Bands were excised from diffusates of Kentucky blue...

Ngày tải lên: 13/08/2014, 13:20

... patterns of muscle proteins probably overestimate the presence of soluble proteins and underestimate the expression of membrane-associated proteins Despite these problems, the proteomics analysis of ... viscosity of the extract as a result of DNA, lL of DNase I (200 units) was added per 100 lL of buffer [30] Following filtration through two layers of miracloth and the addition of four volumes of ice-cold ... combination of the pI value and molecular mass of individual 2D spots can be useful in the initial identification of proteins However, owing to the abnormal electrophoretic mobility of certain proteins, ...

Ngày tải lên: 19/02/2014, 16:20

... Fe-protein; B, modified Fe-protein Fig Analysis of both nitrogenase Fe-protein isoforms from conventional Coomassie stained SDS ⁄ PAGE gels by MALDI-TOF MS MALDITOF spectrum of the unmodified Fe-protein ... °C until analysis by MS MALDI-TOF analysis For MALDI-TOF analysis, peptides were resuspended in 10 lL of 50% ACN, diluted : 10 with ultrapure bidest H2O and mixed with an equal volume of matrix ... effective for the analysis of other types of modifications on nitrogenase Fe -proteins Probably, it also provides a new method for the investigation of other labile modifications on proteins resulting...

Ngày tải lên: 07/03/2014, 02:20

... Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined by inductively ... 0.12 85.4 a Average of two determinations; b app (quinoline) (mM) kcat app (quinoline) (s)1) average of three experiments; c [1] Metal content of the Qor proteins and analysis of nucleotides released ... organic part of the molybdenum cofactor However, we cannot exclude that the pyranopterin part of the cofactor is somehow defective in Qor from strain KT2440 pUF1 UV/Visual spectra of the Qor proteins...

Ngày tải lên: 17/03/2014, 10:20

... Patterns of EIAV Are Closer to Those of Influenza than of disorder patterns of HIV/SIV Analysis of Figure reveals that the pattern of the predicted disorder in EIAV matrix protein is closer to that of ... behavior of the matrix proteins [3] Conclusion Matrix Disorder of Retroviruses Varies with Nature of the Virus A peculiar finding of this paper is the pattern of predicted disorder of EIAV p15 ... Figure PONDR/B-factor plots of matrix proteins of various viruses A) the influenza A M1 protein B) the SIVmac P17 protein PONDR/B-factor plots of matrix proteins of various viruses A) the influenza...

Ngày tải lên: 20/06/2014, 01:20

... present in the mountains of the Alps, the Pyr6n6es, the C6vennes and the Massif Central The genetic variability of the Corsican mouflon has been assessed mainly by means of electrophoretic analyses, ... wild-living) by blood typing and by electrophoresis of transferrin and hemoglobin The aim of this study was to determine the effects of founder events variability of introduced mouflon populations Genetic ... classification Group compositions group correspondence analysis according on to the method of Roux performed on Biomeco software (Lebreton et al, RESULTS Blood proteins Table II provides the allelic frequencies...

Ngày tải lên: 09/08/2014, 18:21

... http://www.biomedcentral.com/1471-2229/11/33 Page of 16 Figure Representation of genomic loci of TLP genes in the genome of Populus trichocarpa ’Nisqually-1’ (A), Position of TLP genes on scaffold Transposable element coverage of the ... up-regulation of many PR proteins [26] In particular, transcript profiling of poplar leaves during an incompatible interaction (i.e host-specific resistance) with M larici-populina established a set of ... neo-functionalization of paralogs [37] Analysis of natural selection is increasingly used in plant pathology to estimate how evolutionary forces impact genes and corresponding proteins, from the scale of amino...

Ngày tải lên: 11/08/2014, 11:21

... 1385.2 1397.0 B Mass (m/z) C D Figure Identification of methylation of Lysine 36 of histone variant H3.2 Identification of methylation of Lysine 36 of histone variant H3.2 A MALDI-TOF mass spectrum ... Identification of the two variants of histone H4 Identification of the two variants of histone H4 A MALDI-TOF mass spectrum showing that the amount of the peptide with calculated mass of m/z 1003.6 ... a flow rate of 0.3 μl/min Each fraction was autocollected on the MALDI-TOF sample plate Mass spectrometry Mass spectrometric analysis was carried out using a MALDI-TOF/TOF tandem mass spectrometer...

Ngày tải lên: 12/08/2014, 03:21

... BTB proteins Distribution ofprevious page) in eukarytoic genomes Distribution of BTB proteins in eukarytoic genomes (a) Representation of BTB proteins in selected sequenced genomes Twelve of the ... the Skp1 and ElonginC proteins, 52% of the C elegans BTB proteins, but only 17% of the human proteins, belong to this family There may be additional domains in some of these proteins that have yet ... Many members of this widely represented family of proteins are implicated in the stability and dynamics of actin filaments [75-78] With few exceptions, all of the 515 BTB-kelch proteins in our...

Ngày tải lên: 14/08/2014, 14:22

... 25 2.4.2 The role of Osh proteins in maintaining sterol homeostasis………………… .29 26 2.4.3 The role of Osh proteins in membrane trafficking…………………………………30 27 2.4.4 Roles of Osh proteins in other ... targeting of OSBP proteins 2.5.1 Multiple membrane-targeting of OSBP proteins ………………………… … 35 30 2.5.2 Requirement of phospholipids for membrane targeting ……………………… …37 32 2.5.3 Regulation of membrane ... loss of Osh proteins may be consequences of disrupted lipid homeostasis However, current studies could not delineate the molecular functions of individual Osh proteins 2.5 Membrane targeting of...

Ngày tải lên: 16/09/2015, 08:31

... spectrum The peaks of interest where selected manually using the built-in function for peak picking in XMASS The masses of the fragments were compared to lists of fragment ion masses generated ... experimental masses agree well with the theoretical masses of the candidate peptides Mass measurement errors at the p.p.m.-level were typically observed Molecule Theoretical mass (Da) Measured mass (Da) ... identity of the peptide, and hence the presence of des-(27–31)C-peptide in extracts of mouse pancreatic islets Two other signals of importance were detected whose masses agreed with those of glucagon...

Ngày tải lên: 17/03/2014, 10:20

... 1607.0 Figure Mass analysis of a distinct peptide from the G3L protein using Method (MALDI-TOF/TOF) Mass analysis of a distinct peptide from the G3L protein using Method (MALDI-TOF/TOF) The membraneenriched ... Figure Mass analysis of a distinct peptide from the L3L protein using Method (LC-ESI-Q-TOF MS) Mass analysis of a distinct peptide from the L3L protein using Method (LC-ESI-Q-TOF MS) The core- enriched ... ionization tandem mass spectrometer with time -of- flight/time -of- flight optics (MALDI-TOF/TOF), 2.) a quadrupole-time of flight mass spectrometer (LC-ESI-Q-TOF), and 3.) a quadrupole ion trap mass spectrometer...

Ngày tải lên: 19/06/2014, 08:20

... 1607.0 Figure Mass analysis of a distinct peptide from the G3L protein using Method (MALDI-TOF/TOF) Mass analysis of a distinct peptide from the G3L protein using Method (MALDI-TOF/TOF) The membraneenriched ... Figure Mass analysis of a distinct peptide from the L3L protein using Method (LC-ESI-Q-TOF MS) Mass analysis of a distinct peptide from the L3L protein using Method (LC-ESI-Q-TOF MS) The core- enriched ... ionization tandem mass spectrometer with time -of- flight/time -of- flight optics (MALDI-TOF/TOF), 2.) a quadrupole-time of flight mass spectrometer (LC-ESI-Q-TOF), and 3.) a quadrupole ion trap mass spectrometer...

Ngày tải lên: 20/06/2014, 04:20

... flour The gluten proteins are made up of gliadins, a heterogeneous collection of monomeric proteins that are associated with extensibility properties, and glutenins, a group of proteins that confer ... sequences Proteins within each subgroup have distinct N-terminal and C-terminal sequences The structures of the proteins and the number and arrangement of cysteine residues within proteins of each ... identities of individual gluten proteins using approaches that work for most other proteins, in part because the gluten proteins yield relatively few peptides of a size suitable for MS/MS analysis...

Ngày tải lên: 12/08/2014, 03:21

... proteomic analysis of C3 and C3b samples 51 2.3.1 Protein cross-linking for quantitative analysis 51 2.3.2 Sample preparation for mass spectrometric analysis 52 IV 2.3.3 Mass spectrometric analysis ... for cross-linked peptides 1.4 Analysis of cross-linked peptides by mass spectrometry 1.4.1 Mass spectrometric analysis of cross-linked samples Peptide mixtures of digested cross-linked protein ... 3D proteomics: Analysis of proteins and protein complexes by chemical cross-linking and mass spectrometry Zhuo A Chen Thesis for the Degree of Doctor of Philosophy The University of Edinburgh August...

Ngày tải lên: 30/09/2015, 05:51

... peptide is thus 6073 Da The measured mass is 363 mass units higher than predicted Further analysis of c¢-type ions (singly and doubly ETD-MS analysis of peptides and proteins charged) indicates that ... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of ... breakthrough in the field of proteomics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer The scan times required for direct analysis of proteins using ETD...

Ngày tải lên: 18/02/2014, 16:20

... intensity 0.8 0.6 0.4 0.2 min Fig Time-lapse LSM analysis of OlvasGFP reveals the dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed ... suggesting the existence of some interactive cellular partner FRAP analysis of the nuage revealed the slow recovery of OlvasGFP (Fig 3B) When the whole part of the single compartment of nuage was bleached, ... in the part of the cytoplasm outside of the granules FRAP analysis in the granules provided typical recovery curves of these fusion proteins: a diffusion constant with two components of 0.9 and...

Ngày tải lên: 18/02/2014, 16:20

... distribution of the size of the inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent on when the analysis ... percentage of the cell population with an FL2-H fluorescence greater than · 101 was recorded during the FACS analysis The percentage of decrease of DYm was calculated as the ratio of the percentage of ... formation of carbonyl residues on amino acid side chains of proteins [43,64] In order to explore the ability of httEx1-polyQ to oxidize cellular proteins, we performed immunoblot detection of protein...

Ngày tải lên: 19/02/2014, 06:20

... K Analysis by Sacramento County Office of Education (format adapted from Monterey COE), June 2010 © Sacramento County Office of Education, California, 2010 All rights reserved 16 Analysis of California
ELA
standards
to
Common Core standards­1st
grade ... on
knowledge of sound­letter
 relationships.
 English Language Arts, Grade K Analysis by Sacramento County Office of Education (format adapted from Monterey COE), June 2010 © Sacramento County Office of ... Grade K Analysis by Sacramento County Office of Education (format adapted from Monterey COE), June 2010 © Sacramento County Office of Education, California, 2010 All rights reserved 13 Common Core Standards
not
found
in
Kindergarten
CA
ELA
Standards

...

Ngày tải lên: 24/02/2014, 18:20