marker for microbial mass protein 53 with some modification as described in 13
In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
Ngày tải lên :
15/03/2014, 04:20
... to the methods involved in collecting, preparing and screening plants for bioactive properties for use in manipulating key ruminal fermentation pathways and against gastrointestinal pathogens ... directly by using a stereo microscope to examine the washings/contents collected from the abomasa, small intestines or large intestines Materials • • • • Abomasum or small or large intestine from ... direct anthelmintic effects in these assays These include condensed tannins, saponins, flavonols, lectins and various proteases However, since most of these assays operate best within a narrow pH...
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ECOLOGY OF COLD SEEP SEDIMENTS: INTERACTIONS OF FAUNA WITH FLOW, CHEMISTRY AND MICROBES potx
Ngày tải lên :
15/03/2014, 19:20
... (Levin et al 2003) Vertical partitioning of seep sediments was investigated for dorvilleid species in the Eel River Basin Whereas no partitioning was evident for five dorvilleid species in microbial ... has been limited quantitative sampling of associated fauna In uence of seep clams on associated infauna is discussed later in the section on macrofauna Seep infauna Foraminifera Interest in Foraminifera ... (Phalascosoma) Densities of many taxa increase with habitat complexity, measured as tubeworm density, but decline with age of the tubeworm aggregation Increasing patch age leads to a decline in...
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INTERACTIONS OF POLYMERS WITH FIBRILLAR STRUCTURE OF CELLULOSE FIBRES: A NEW APPROACH TO BONDING AND STRENGTH IN PAPER docx
Ngày tải lên :
18/03/2014, 02:20
... II) In contrast to xyloglucan and CMC, addition PDADMAC induced a sharp increase in frequency and a decrease in dissipation, indicating a reduction of the detected mass and an increase in elasticity ... able to reinforce the natural bonding of fibres Expectedly, the reinforcing mechanism also include the self-crosslinking of the polymers, at least to some extent, since the crosslinking reactions ... (2006), the pulp was ground with a Masuko Supermasscolloider (Masuko Sangyo, Japan) The grinding produced fines mainly from the primary and the S1 cell wall layers, and, after washing, even fibrillation...
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Báo cáo khoa học: Surface-enhanced vibrational spectroscopy for probing transient interactions of proteins with biomimetic interfaces: electric field effects on structure, dynamics and function of cytochromec doc
Ngày tải lên :
22/03/2014, 16:20
... rate constants [28] With increasing field strength, protein reorientation is increasingly restricted, due to an electric field-dependent increase in the activation barrier for the transition SERR ... the medium-affinity binding domain involving Lys25 and Lys27 As long as the rotational diffusion of the protein on the SAM surface is fast in comparison with electron tunnelling, electron transfer ... thickness, using cytochrome c as a model protein [25,26,28–37] It was found that, with decreasing distance, the electron transfer rate constant first increases exponentially, as expected for long-range...
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Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf
Ngày tải lên :
23/03/2014, 13:20
... overexpressed was the opposite of that observed for the case when dUSP was overexpressed As shown in Fig 4D, as the amount of plasmid overexpressing EcR was increased progressively, the fold induction ... acting dose of L314R-expressing plasmid (that suppressed JH III-activation), but were also cotransfected with increasing amounts of plasmid expressing wtUSP The outcome was that the increasing ... protein (GFP)-dUSP fusion protein was prepared in pIE1-4 by subcloning the GFP coding sequence for into the EcoRI ⁄ SmaI sites of the pIE14 vector, upstream of and in the same reading frame as...
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Báo cáo y học: "Complex genetic association of 6q23 with autoimmune rheumatic conditions" docx
Ngày tải lên :
09/08/2014, 14:20
... be required as initial steps in determining its potential role in modulating PTPN11 expression An alternative explanation is that a polymorphism within an enhancer sequence in the intergenic region ... understanding of the biological basis of the genetic associations of this region with autoimmune rheumatic diseases Competing interests The authors declare that they have no competing interests ... PTPN11, leading to autoimmune disease Determining whether the pseudogene is transcribed and whether sequence variants of the transcript are present on diseaseassociated haplotypes will therefore be...
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Interactions of viologens with conducting polymers, metal salt solutions and glucose oxidase
Ngày tải lên :
16/09/2015, 17:13
... generalized formula as shown in Figure 2.7, where some of the C6H4 rings are in the benzoid form while others in the quinonoid form Aniline polymers are actually poly(p-phenyleneimineamine)s, in which ... treated with a base to form EB The leucoemeraldine base is off-white in powder form and forms thin transparent coatings It can be conveniently prepared by the reduction of the EB powder or coating, ... were to form a layer on the electrode surface by self-assembly of a viologen with other species, or in which the viologen is occluded within a ‘binder’, ensuring that the viologen remains in the...
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Tài liệu Báo cáo khoa học: Comparison of a coq7 deletion mutant with other respiration-defective mutants in fission yeast doc
Ngày tải lên :
18/02/2014, 14:20
... apt1 occurred in all ubiquinone-deficient strains, induction of gpx1+ was not observed in any of the tested strains (Fig 6) However, in the wild-type strain treated with mm H2O2 for 15 min, a high ... Localization of Coq7 in Sc pombe was examined by constructing a Coq7–green fluorescent protein (GFP) fusion Whereas GFP alone localized in the cytoplasm, the Coq7–GFP fusion protein localized in mitochondria ... normal-phase TLC with authentic ubiquinone-10 as a standard Normal-phase TLC was carried out on Kieselgel 60 F254 with benzene The band containing ubiquinone was collected from the TLC plate following...
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Báo cáo khoa học: The capsid protein of human immunodeficiency virus: interactions of HIV-1 capsid with host protein factors ppt
Ngày tải lên :
07/03/2014, 00:20
... CyPA binding loop C-term N5 C3 N4 N7 C4 Interdomain linker N6 N3 C1 N term N-term reaction, to generate the correct conformation of proline, which is a rate-limiting step in protein folding This ... cyclophilin binding loop, as the specific binding site for CyPA [30,32,38–40] (Figs 2B and 3) Two other binding sites on CA, specifically GP157 and GP224, with higher affinities than the GP90 site within ... Mascarenhas and K Musier-Forsyth R55 P90 CyPA binding loop (HAGPIA) CA-NTD Cyclophilin A (CyPA) multimerize were deficient in CyPA binding CyPA binding to mature CA causes structural changes in...
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Báo cáo khoa học: Interactions of imidazoline ligands with the active site of purified monoamine oxidase A potx
Ngày tải lên :
07/03/2014, 10:20
... ⁄ guanidinium binding domains on monoamine oxidases ) relationship to subtypes of imidazoline-binding proteins and tissue-specific interaction of imidazoline ligands with monoamine-oxidase-B J ... Imidazoline binding in the MAO-A active site T Z E Jones et al Table Binding and inhibition constants for I2 ligands with MAO-A The Ki values for purified human MAO-A were determined as described in ... site before each docking The docking procedure was tested using clorgyline as a control for comparison with the original structure When the covalent bond was present, the optimum position was in...
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Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
Ngày tải lên :
07/03/2014, 10:20
... motifs in caspase-1, caspase-3, caspase-7, caspase-8 and caspase-10 gene upstream sequences Sequence name Strand Start End Match type Motif sequence ENSG0000 0137 752 Caspase-1 ENSG00000164305 Caspase-3 ... motifs identified in the caspase-1 (for reference) caspase-3, caspase-7, caspase-8 and caspase-10 genes are shown in the Table In Table 3, the start and end positions of the motifs are indicated by ... could interact with the putative promoter sequences of the caspase-8 and caspase-10 genes Expression of caspase-8 and caspase-10, as detected by semiquantitative RT-PCR, was also increased in the...
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Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx
Ngày tải lên :
07/03/2014, 16:20
... measurements of decorin binding to immobilized methylated type I collagen (A) Interaction with wild-type decorin core protein (obtained by chondroitin ABC lyase digestion) in HBS Decorin core protein ... of wild-type decorin core protein used were as indicated (B) Interaction with wild-type decorin in HBS buffer (solid lines) and in HBS buffer containing 15 lM ZnCl2 (dotted lines) Concentrations ... core protein concentrations were as indicated (B) Interaction with wild-type decorin in HBS buffer (solid lines) and in HBS buffer containing 15 lM ZnCl2 (dotted lines) Wild-type decorin concentrations...
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Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf
Ngày tải lên :
08/03/2014, 23:20
... increase in the overall signal (as the three proteins behave as heat shock proteins) Surprisingly, when the eluted fractions were assayed for neutral trehalase, no enzyme activity was found associated ... trehalase in slab gels was expressed as enzyme units per mg protein Protein determination was performed by absorbance measurement at 280 and 205 nm according to the method described in previously [24] ... molecular mass trehalose synthase–trehalase complex or linked to forms of lower molecular mass as for neutral trehalase, although accumulation of trehalose by ntp1-deleted strains seems to indicate...
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Đề tài " On the periods of motives with complex multiplication and a conjecture of GrossDeligne " pdf
Ngày tải lên :
14/03/2014, 22:20
... conjecture described above relies on this last theorem; we write out the fixed-point formula for the de Rham complex and obtain a first formula (11) which involves differential-geometric invariants (in ... author in [13] More precisely, we write down the fixed-point formula as applied to the de Rham complex of a variety equipped with the action of a finite group This yields a formula for some linear ... the case where M is a submotive of an abelian variety with complex multiplication by Q Indeed, assuming the Hodge conjecture, one can show by examining its associated Hodge structures that some...
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Báo cáo khoa học: Interactions of elongation factor EF-P with the Escherichia coli ribosome doc
Ngày tải lên :
16/03/2014, 06:20
... protein was labeled by formylation with [14C]formate and the protein was bound to partly dissociated 70S ribosomes containing 30S and 50S subunits, as described in Experimental procedures The ribosomeÆ[14C]EF-P ... using FLPC Fractions were assayed after each step to locate the protein The purified protein was concentrated and was stored at )80 °C Protein concentration was determined using the method described ... unknown It has been proposed that these proteins stimulate the synthesis of certain proteins in the cell [19] In mammalian cells, including in humans, the genes encoding eIF-5A and its isoform, eIF-5A2,...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
Ngày tải lên :
16/03/2014, 23:20
... Ôarms domainÕ of CLR, which contains the binding site of the tetramer C1r2–C1s2 It has been reported that basic residues lysine and arginine in the hinge region are involved in the assembly of ... when C1 instead of C1q was used in this competition experiment with fucoidan, a lower inhibition of the protein binding to DNA was obtained (for the same C1/fucoidan molar ratio : 100), as colocalization ... fucoidan interacts with C1q In order to ascertain this binding property of fucoidan, we performed competition experiments between fucoidan and DNA for the binding to C1q Fluorescent T4 DNA was incubated...
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Báo cáo khóa học: The proteasome inhibitor, MG132, promotes the reprogramming of translation in C2C12 myoblasts and facilitates the association of hsp25 with the eIF4F complex pptx
Ngày tải lên :
23/03/2014, 12:20
... X-100 for min, rinsed once in NaCl/Pi then incubated with 0.1% (w/v) NaBH4 for prior to three washes with NaCl/Pi Nonspecific binding was blocked by adding 1% BSA in NaCl/Pi for 30 Cells were incubated ... protease inhibitor mix – EDTA), the resin was washed and further incubated for 20 on ice in Buffer F (Buffer E, but The proteasome inhibitor, MG132, promotes an inhibition of protein synthesis without ... before the addition of recombinant hsp25, as described in Materials and methods Following isolation and washing of the resin, protein was eluted and visualized by SDS/PAGE and Coomassie staining...
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Báo cáo khoa học: Characterization of the interactions of the nephrin intracellular domain Evidence that the scaffolding protein IQGAP1 associates with nephrin potx
Ngày tải lên :
23/03/2014, 13:20
... The peptide mass table was used in ProFound to scan the nonredundant sequence database (NCBInr) for matching proteins Amino acid sequencing For obtaining amino acid sequence, Nano-ES mass spectra ... other known proteins It contains a few very typical consensus sequences found in the most proteins, such as two putative phosphorylation sites for protein kinase C and three for casein kinase II However, ... and its interaction with nephrin has been examined also in vitro, although the mapping of interacting domain for nephrin binding resulted in controversial reports [27,28] Studies employing gradient...
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Báo cáo Y học: Chimeric receptor analyses of the interactions of the ectodomains of ErbB-1 with epidermal growth factor and of those of ErbB-4 with neuregulin potx
Ngày tải lên :
24/03/2014, 03:21
... corresponding to domain III showed the highest affinity for EGF [20] Nevertheless, it has been reported that domain I as well as domain III is involved in EGF binding, suggesting bivalent binding of EGF ... deletion in the N-terminal region of domain I impaired the EGF binding of ErbB-1 [21] The N-terminus of EGF was linked to Tyr101 in domain I by using a covalent cross-linking reagent [22] Chimeras ... agrees with the previously reported conclusions, that EGF binding to the receptor depends mainly on domain III and less on domain I in the bivalent binding [18] Therefore, binding assays using the...
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Báo cáo khoa học: Interactions of the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus pot
Ngày tải lên :
30/03/2014, 20:20
... of arginine residues at binding interfaces Indeed, in a database analysis of the contributions of individual amino acid residues to protein protein interactions [20], arginine appears with a ... effects, as described in detail elsewhere [12] The replacement of Arg135 in the PSBD with alanine (R135A) causes a significant decrease in the binding affinity of PSBD for E3 [13] Indeed, Arg135 plays ... explained In mutagenesis studies, lysine is usually regarded as the best substitute for arginine in conserving the positively charged side-chain and many binding proteins appear to be unaffected by interchanging...
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