manufacturer s instructions total rna 2µg from caudal neural tubes of embryos was added with 1µl of dntp mix 10mm 1µl of oligo dt primer 0 5µg µl and depc treated

Trinucleotide (CAG) repeat polymorphism of the androgen receptor gene in human disease

Ngày tải lên : 11/09/2015, 09:05

... UNIVERSITY OF SINGAPORE 200 4 This thesis is dedicated to Santhosh, Roger, Jolanda and my parents This thesis is mine as well as yours ACKNOWLEDGEMENTS Firstly and mostly I will like to thank my supervisor ... function is to mediate the transcriptional response in target cells in response to ligands such as the sex steroids (progestins, estrogens and androgens), adrenal steroids (glucocorticoids and mineralocorticoids), ... aggregates in all of those disorders including SBMA These aggregates are similar to those found in other neurodegenative disorders such as Alzheimer s, Parkinson s disease and prion diseases, suggesting...

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GT-repeat polymorphism in the heme oxygenase1 gene promoter and the risk of carotid atherosclerosis related to arsenic exposure ppt

Ngày tải lên : 10/08/2014, 05:21

... allele 100 - 300 , Non-carriers of the class S allele 100 - 300 , Carriers of the class S allele > 300 , Non-carriers of the class S allele > 300 , Carriers of the class S allele (3.1) 20 (7.8) (2.4) 1 .00 ... the class S allele, % Lanyang cohorta ≤ 50, Non-carriers of the class S allele ≤ 50, Carriers of the class S allele 50- 100 , Non-carriers of the class S allele 50- 100 , Carriers of the class S allele ... risk of atherosclerosis from carriers to non-carriers of the class S allele was also measured by arsenic exposure All analyses were performed using SAS (Win8e; SAS, Cary, NC, USA) statistical software,...

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Shorter GT repeat polymorphism in the heme oxygenase-1 gene promoter has protective effect on ischemic stroke in dyslipidemia patients potx

Ngày tải lên : 10/08/2014, 05:21

... the previous reports[23-25,34,35] The previous studies defined L and S alleles first and constructed genotypes SS, SL, and LL to examine the disease risk We demonstrated the results assuming co-dominant ... on HDL-C was shown The associations between stroke status and HO-1 genotypes were examined by stratifying on stroke and cardiovascular (CVD) risk factors The risk of odds ratio (OR) was showed ... selection bias, such as survivor for severe disease It is less considered in previous studies Interestingly, we also described more risk effects of genotype L on stroke in those with normal TC...

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Báo cáo y học: "Association of the D repeat polymorphism in the ASPN gene with developmental dysplasia of the hip: a case-control study in Han Chinese" potx

Ngày tải lên : 12/08/2014, 15:22

... Recently, an aspartic acid repeat polymorphism of ASPN was first described as an osteoarthritis-associated polymorphism The D14 allele of ASPN was over-represented in osteoarthritis subjects, and D14 ... 3 .03 0. 71 0. 004 0. 56 to 0. 90 2. 10 9.3* 10- 4 1.34 to 3.29 Male patients (n = 57) vs Male controls (n = 155) 1 .02 0. 96 0. 39 to 2.68 0. 51 0. 002 0. 33 to 0. 79 1.34 0. 55 0. 50 to 3.59 ASPN, asporin; CI, ... Lack of association of a variable number of aspartic acid residues in the asporin gene with osteoarthritis susceptibility: case-control studies in Spanish Caucasians Arthritis Res Ther 200 6, 8:R55...

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Báo cáo y học: "Polymorphism in the tumour necrosis factor receptor II gene is associated with circulating levels of soluble tumour necrosis factor receptors in rheumatoid arthritis" ppsx

Ngày tải lên : 09/08/2014, 07:20

... damage was measured by scoring radiographs of the hands and feet using the method proposed by Larsen and coworkers [32], and functional outcome was assessed using the Health Assessment Questionnaire ... package for Windows (NCSS 200 0, NCSS Statistical Software, Kaysville, Utah, USA) P < 0. 05 were considered statistically significant Results sTNFR levels in rheumatoid arthritis Both sTNFRs were detected ... relationship between the two sTNFRs was assessed using Spearman 's rank correlation, whereas differences in sTNFR levels between early and established RA were assessed using the Mann–Whitney U-Test...

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Báo cáo y học: "A polymorphism in the human serotonin 5-HT2A receptor gene may protect against systemic sclerosis by reducing platelet aggregation" potx

Ngày tải lên : 09/08/2014, 13:21

... allele was observed in SSc patients (χ2 = 7. 308 , one degree of freedom, P < 0. 001 ) All of the results were confirmed after the 1 ,00 0-fold permutation test (P < 0. 05 and P < 0. 001 for genotypes and ... (SPSS Inc., Chicago, IL, USA) P < 0. 05 was considered significant Continuous values are expressed as the mean ± standard deviation – except for skewed distributions (skewness 2), ... 0. 29 0. 19 0. 05 0. 66 0. 46 0. 33 0. 01 0. 91 0. 82 0. 72 0. 001 0. 99 0. 98 Our results indicate that the C/T transition at position +1,354 of the third exon of the 5-HTR2A gene [19] is associated with a...

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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt

Ngày tải lên : 16/03/2014, 18:20

... 200 300 200 100 80 100 80 B +1 Exon RPAAS Intron (18.4 Kb) xon MMCB1 ATG RPA-1 RPA-2 CB1: GG GTAAGA TAG GGTT Conserved: AG GURAGU YAG RNNN C D L P N 7 50 500 400 300 200 P * N 500 400 300 200 ... lateral striatum where the highest levels of expression of CB1 are found and the mutant huntingtin- Ratio of Mature CB1/HPRT A 1 .00 0. 80 0. 60 * 0. 40 0. 20 Ratio of Primary CB1/HPRT B 12 0. 08 0. 06 0. 04 ... reactions, 10 pg of undigested probe control (P) and a no target control that consists of 400 pg of probe combined with excess yeast RNA and digested with RNase (N) are shown In C, 10 lg wild-type striatal...

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Báo cáo Y học: Amino acids 3–13 and amino acids in and ﬂanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf

Ngày tải lên : 17/03/2014, 10:20

... pr28/29AA, and pr 30/ 31AA sense and antisense oligonucleotides were modiﬁed pr17–32 sense and antisense oligonucleotides, containing GCTGCA (sense) and TGCAGC (antisense) as two adjacent alanine codons ... frame insertion of a double-stranded oligonucleotide in the corresponding sites of pGEX-2TK (Amersham Biosciences, Uppsala, Sweden) Oligonucleotides were PDsense and PDantisense Insertion of the ... agarose beads were washed ﬁve times with buffer A supplemented with 0. 1 M NaCl with or without 100 nM R1881, boiled in 30 lL Laemmli sample buffer and 25 lL supernatant was separated over a 10% SDS/PAGE...

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Báo cáo y học: "No evidence for an association between the -871 T/C promoter polymorphism in the B-cell-activating factor gene and primary Sjögren''''s syndrome" pptx

Ngày tải lên : 09/08/2014, 07:20

... increased serum and salivary expression of BAFF in patients with pSS [2-6] Moreover, because one-third of first-degree relatives of patients with primary pSS suffer from other autoimmune diseases ... autoantibodies, in whom disease is usually less systemic Moreover, we previously reported that BAFF level was not associated with systemic features in pSS [8] Likewise, no association was found between ... Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren 's syndrome J Clin Invest 200 2, 109 :59-68 Jonsson MV, Szodoray P, Jellestad S, Jonsson R, Skarstein K: Association...

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Báo cáo y học: "Replication of association of the D-repeat polymorphism in asporin with osteoarthristis" docx

Ngày tải lên : 09/08/2014, 08:22

... that this estimation is valid because the inclusion criteria are the same, provided that the ethnicity is consistent as the Spanish group itself proposed If so, the association of asporin has been ... Caucasians, there was no evidence for an important effect of the asporin D repeat polymorphism; this was similar to the conclusion of the authors of the UK study [3] Our conclusion was based in ... Kaliakatsos M, Tzetis M, Kanavakis E, Fytili P, Chouliaras G, Karachalios T, Malizos K, Tsezou A: Asporin and knee osteoarthritis in patients of Greek origin Osteoarthritis Cartilage 200 6, 14: 609 -611...

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Báo cáo y học: "A case-control study of rheumatoid arthritis identifies an associated single nucleotide polymorphism in the NCF4 gene, supporting a role for the NADPH-oxidase complex in autoimmunity" doc

Ngày tải lên : 09/08/2014, 10:21

... 0. 87 0. 20 to 0. 60 0 .00 01 2 .03 0. 88 to 5.29 0. 15 All 0. 86 0. 70 to 1 .07 0. 17 1 .01 0. 61 to 1.71 0. 98 1 .02 0. 80 to 1.31 0. 88 1.12 0. 63 to 2 .00 0. 70 Men 0. 54 0. 36 to 0. 83 0. 003 0. 79 0. 28 to 2.24 0. 65 ... 1.62 0. 86 0. 94 0. 75 to 1.17 0. 56 1 .01 0. 60 to 1.71 0. 97 0. 50 0.35 to 0. 73 0. 000 2 1. 20 0.52 to 2.81 0. 67 All 0. 85 0. 69 to 1 .04 0. 12 0. 92 0. 56 to 1.53 0. 76 Women 0. 96 0. 75 to 1.23 0. 74 1 .03 0. 58 ... Caucasians used for validation or in the EIRA cohort (rs1 804 006 [CYBA], rs789183, rs13 306 581 and rs13 306 575 [NCF2], and rs1 305 7 803 , rs 100 3 501 , rs12158689, rs96 105 95, rs 207 2 706 and rs 207 2711 [NCF4];...

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Báo cáo y học: "Association of the T allele of an intronic single nucleotide polymorphism in the colony stimulating factor 1 receptor with Crohn''''s disease: a case-control study" ppt

Ngày tải lên : 11/08/2014, 10:23

... was transferred to a clean tube and 100 ul of chloroform were added (Sigma) The sample was centrifuged at 10, 000 × g for and the aqueous phase transferred to a clean tube and mixed with 200 µl ... pellet was resuspended in 85 µl of pellet buffer ( 10 mM Tris-HCL, ph 8 .0, 10 mM EDTA, pH 8 .0, 1 50 mM NaCl) followed by µl of Proteinase K ( 20 mg/ml) (Invitrogen, Grand Island, NY) and 10 µl 10% SDS ... Immunohistochemistry Master Mix for PCR was prepared using Taq polymerase, dNTPs and other reagents from Invitrogen (Carlsbad, Table 1: Crohn 's Disease Status vs CSF1R A 203 3T SNP: All Patients CDT...

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Báo cáo y học: "A1298C polymorphism in the MTHFR gene predisposes to cardiovascular risk in rheumatoid arthritis" ppt

Ngày tải lên : 12/08/2014, 12:20

... published Acknowledgements The present study was supported by two grants from Fondo de Investigaciones Sanitarias PI06 -00 24 and PS09 /00 748 (Spain) This work was partially supported by RETICS Program ... Statistical analysis Strength of association between RA and alleles or genotypes of the MTHFR gene polymorphism was estimated using odds ratios (ORs) and 95% confidence intervals (CIs) Levels of significance ... patients with CV events versus 49.7% in those RA patients without CV events; OR = 1.67; 95% CI = 1 .00 to 2.82; P = 0. 04) This association was in keeping with a statistically significant increase of...

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Báo cáo y học: " A polymorphism in the interleukin-4 receptor affects the ability of interleukin-4 to regulate Th17 cells: a possible immunoregulatory mechanism for genetic control of the severity of rheumatoid arthritis" doc

Ngày tải lên : 12/08/2014, 15:22

... × PBS /0. 05% Tween at 200 μl per well and blocked using 200 μl of PBS with 10% fetal calf serum per well for hours The plates were then washed three times with 200 μl of × PBS /0. 05% Page of Tween ... addition of brefeldin A ( 10 μg/ml) for more hours The cells were washed and × 106 cells per sample were used for staining Cells were first blocked with 20 μl of 10% human serum/ 10% mouse serum in PBS ... well The standard curve was created in duplicate starting with a concentration of 2 ,00 0 pg/ml and serial twofold dilutions to 7.8 pg/ml Supernatants and sera were assayed in triplicate at 100 μl...

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Báo cáo khoa học: "Septic shock is correlated with asymmetrical dimethyl arginine levels, which may be influenced by a polymorphism in the dimethylarginine dimethylaminohydrolase II gene: a prospective observational study" docx

Ngày tải lên : 13/08/2014, 03:20

... alleles at DDAH II -449 Parameter ADMA day data ADMA day data 0. 31 (0. 000 2) 0. 32 (0. 001 ) 0. 13 (0. 02) 0. 24 (0. 005 ) 0. 29 (0. 000 3) 0. 20 (0. 01) WCC ns ns IL-6 ns 0. 25 (0. 006 ) pH Base excess Lactate ... 1.8 (1 .05 –3.75) 0. 018 Base excess -3.5 (-9 to 3.4) -2 (-4.95 to 0. 55) ns SOFA (8–12) (3.25–4.75)

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Báo cáo sinh học: " Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene" pptx

Ngày tải lên : 14/08/2014, 13:22

... 0. 118A at buttock (cm) 2.776 ± 0. 090a 2.782 ± 0. 065a 2.843 ± 0. 139ab 3 .03 9 ± 0. 094b 2.851 ± 0. 068ab 2 .02 1 ± 0. 103 a 1.913 ± 0. 075A 1.8 60 ± 0. 158a 2.336 ± 0. 107 Bb Microsatellite polymorphisms of ... pigs, we observed no statistically signiﬁcant diﬀerences in CA3 mRNA expression (Fig 1) as shown by t-test analysis (P > 0. 05) An expression pattern was also performed on the skeletal muscle of ... ± S E) AB (155) AA (1 20) 91.122 ± 0. 371 3.671 ± 0. 069ab ab 2.118 ± 0. 064a 90. 445 ± 0. 326 3.781 ± 0. 061a a 2 .01 7 ± 0. 056ab BB (45) 92.162 ± 0. 605 b 3. 503 ± 0. 112b 1.839 ± 0. 104 b associations with...

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The androgen receptor centric transcriptional network in prostate cancer

Ngày tải lên : 09/09/2015, 10:14

... aggressive, metastatic and castrate-resistant form through a variety of mechanisms (Feldman and Feldman, 200 1) Median survival rate for such cases is only 1-2 years (Lassi and Dawson, 200 9) 1.2 Androgens ... et al., 200 7) This particular class of transcription factors was termed as pioneering factors Their presence at these cis-regulatory elements was usually shown to be necessary for subsequent recruitment ... partners and/ or by post translation modifications such as phosphorylation and ubiquitination during activation of cellular signaling (Chakrabarti et al., 200 0; Li et al., 200 0; Sharrocks, 200 1; Wasylyk...

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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

Ngày tải lên : 07/03/2014, 01:20

... were shown to serve as a link between STARS and SRF In NIH 3T3 cells cotransfected with expression plasmids encoding MRTFs and STARS, the MRTFs are translocated to the nucleus in the absence of serum ... Identiﬁcation and characterization of cvHsp A novel human small stress protein selectively expressed in cardiovascular and insulin-sensitive tissues J Biol Chem 274, 36592–36 600 Ghosh A & Heston WD ( 200 4) ... downstream of STARS, and it seems possible that ABLIM integrates signals from the small GTPases, Rac and RhoA (via STARS) toward the actin cytoskeleton Roles of ABPs in the regulation of nuclear...

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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt

Ngày tải lên : 17/03/2014, 23:20

... been shown to inhibit the expression of stress-responsive proteins For example, overexpression of Ku- 70 and Ku- 80 or Ku- 70 alone specifically inhibits HSP 70 expression [56], whereas overexpression ... transfection of Ku- 80 IR gene is consistent with previous report that the stability of Ku- 70 is compromised by the absence of Ku- 80 [57] This is the first report to show that Ku- 80 expression is suppressed ... expression of IR versions of specific genes has advantages over RNAi by directly introduced double-stranded RNA [36] We constructed a Ku- 80 IR vector that was able to synthesize hairpin double-stranded...

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