management of medical emergencies in the oral health care setting
Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... as shown by others [5,17] The complex was stable in 0.5% DDM even after addition of reducing agent (1% b-mercaptoethanol), indicating strong interaction, independent of maintenance of disulfide ... Notch signalling in development in addition to the generation of Ab peptides in pathological processes Study of the characteristics of the PS complex will facilitate elaboration of the molecular ... processes involved in the production of Ab Analysis of these complexes requires detergent solubilization in conditions that not disrupt protein interactions This can lead to loss of weakly interacting...
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Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc
... client signaling protein kinases We next investigated whether the accumulation of phosphorylated Cdc37 in the EGF-induced membrane ruffles was a result of an increase in the phosphorylation of Cdc37 ... line known to show prominent membrane ruffling in response to growth factors [34] KB cells were serum starved by incubating them in medium containing only 1% fetal bovine serum for h and then incubated ... Silverstein AM & Galigniana MD (1999) A model for the cytoplasmic trafficking of signalling proteins involving the hsp90-binding immunophilins and p50cdc37 Cell Signal 11, 839–851 Pratt WB & Toft DO...
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GEL ELECTROPHORESIS – PRINCIPLES AND BASICS pdf
... Staining: The most sensitive staining for protein is silver staining This involves soaking the gel in Ag NO3 which results in precipitation of metallic silver (Ag0) at the location of protein ... in a community and the rate of mixed G genotypes infections In view of the recent introduction of rotavirus vaccine in many countries, the correct identification of the G genotypes involved in ... formulations underlines the importance of virological surveillance, rotavirus strain characterization and the evaluation of the impact of these vaccines in diminishing the diarrhea illness in our region...
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Báo cáo khoa học: "Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites" ppt
... neosporosis Clin Diagn Lab Immunol 1994, 1, 214-221 Bradford MM A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal ... immunoblot analysis of Neospora caninum tachyzoites 143 Fig Analysis of 2-DE and immunoblot profiles of N caninum tachyzoites (A) A total of 335 spots were detected on the 2-DE profile Of these, 64 spots ... pI values Isoform proteins might be separated into chain-like patterns Each spot of the isoforms might be either originated from different gene or the same gene but occurred usually in post translational...
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Báo cáo khoa học: "Effects of buffer system pH and tissue storage on starch gel electrophoresis of allozymes in three tropical tree species" pps
... of inconsistent staining, uninterpretable inheritance patterns, or other problems Many factors may be responsible for these problems (Kephart, 1990) Some of these factors include the nature of ... Therefore the pH affects the charge and separation of enzymes allowing the possibility of increasing the number of zones of enzyme activity It is relevant to note that the R values and f numbers of ... effect of buffer pH (B and H gel gel electrophoresis and electrode buffers) on the resolving power and staining intensities of electromorphs Follwing a conservative approach by altering the pH of the...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
... efficiently determine rejoining without the inference of the apoptosis Figure illustrates the effect of abrogating apoptosis in NP18 cells from a xenograft, showing clear differences between initial and ... program, public domain Java image processing software (http://rsb.info.nih.gov/ij/) Activation of apoptosis in cells growing in monolayer was examined using immunofluorescence by the specific nuclear ... environment (Figure 2B) To further confirm that the origin of apoptosis was the encapsulation in agarose, we investigated the activation of caspase-3 in cells growing as monolayer (in a physiological environment)...
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báo cáo khoa học: " Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis" pdf
... According to the induction of the NO3- assimilation pathway, in the roots of the plants incubated for the last 30 h in the presence of the nutrient, we observed an increase in the accumulation of ... depend on the need of responding to the new nutritional status In leaves, many proteins were found to be (in) directly involved in the photosynthesis reactivation and in the maintenance of the chloroplastic ... reference gel of the software-assisted analysis the values of pI predicted for any given length of the strip Both Mr and pI of the spots of interest were then determined by using software-automated...
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Báo cáo y học: "Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis" pps
... degrees In addition, different colors of nodes indicate different values of K-core the proteins have The "degree" is defined as the number of interactions of a protein with other proteins in the ... proteins within the particular category; n: the total number of proteins within the same category; Nf: the number of flagged proteins in the protein reference database list; N: the total number of ... protein network While the rank is determined as the decreasing ordering of each protein's degree, the first rank which has the highest degree belongs to the most central protein in the network The...
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Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx
... Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Prolactin-inducible ... protein Prolactin-inducible protein Prolactin-inducible protein Prolactin-inducible protein Cystatin S Cystatin S Cystatin SN Hemoglobin beta chain Hemoglobin beta chain Intersectin 2, (splice isoform ... identifying proteins in different biological samples In short, proteins are separated in the first dimension according to their isoelectric points (pI) and then in the second dimension according to their...
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Báo cáo y học: "Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis" pdf
... protein isoforms for further study Overall, a decrease in coagulation-associated proteins, the depletion of protease inhibitors, and an observed increase in intracellular proteins indicative of injury ... remained largely intact Lysis of red blood cells in the post-shock state are illustrated by an increase in both the a and b chains of hemoglobin concurrent with the identification of haptoglobin, ... potentially tipping the protease/ anti-protease balance Finally, the finding of intracellular enzymes such as the A and B isoforms of aldolase, a glycolytic enzyme with actin-binding properties...
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Tài liệu SEXUAL HEALTH EDUCATION A T SCHOOL AND A T HOME: ATTITUDES AND EXPERIENCES OF NEW BRUNSWICK PARENTS pptx
... school Theme #2: Quality of teaching Some parents mentioned the teaching methods used for SHE and the importance of the quality of teaching They indicated that they want their children to have ... to indicate whether they would be interested in attending a SHE workshop for parents if it was offered at their child’s school Fifty percent of parents indicated that they would be interested in ... providing parents with sexual health information before it is disseminated to their child and providing information on how to discuss sexual health topics with their child Involving parents in the...
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Tài liệu Báo cáo khoa học: "Different Structures for Evaluating Answers to Complex Questions: Pyramids Won’t Topple, and Neither Will Human Assessors" docx
... 16.4% of all Other questions, the nugget pyramid assigned a non-zero F-score where the original binary F-score was zero This can be seen in the band of points on the left edge of the plot in Figure ... total of 75 Other questions (one per target) Each series contained 6–9 questions (counting the Other question), with most series containing questions The task employed the AQUAINT collection of ... target (in the opinion of the assessor), but credit was not given for retrieving facts already explicitly asked for in the factoid and list questions The Other questions were evaluated using the...
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Gel electrophoresis
... amount of sample will cling to the walls of the tubes Make sure the entire volume of sample is at the the bottom of the tubes before starting to load the gel • Tap the overlay cover on top of the ... depressed to the first stop, insert the tip into the sample Raise the plunger of the micropipet to withdraw the sample Load the sample into the appropriate well of the gel Discard the tip Repeat ... (negative input) Insert the plug of the red wire into the red input of the power source (positive input) Set the power source at the required voltage and conduct electrophoresis for the length of time...
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Uniform t shirts and company uniforms
... agreement with the organization authorities on the minimum order basis where the customer needs to buy a minimum of specific amount of t shirts It can be the maker to determine the minimum but limit ... must check the standard and also the status from the store before purchasing a uniform shirt stock from him The store should have a minimum of a good number of color designs and fashions of uniform ... http://www.wahmee.com.sg/products/uniforms-corporate-gifts the whole stack of t shirts that have been bought early by having an older design would go waste without having to be used This adds to wastage of company's capital and therefore can...
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Gel Electrophoresis – Teacher Instructions
... when loading the gel; hold the sample-loading device in one hand and use your other hand and arm to support and stabilize the one doing the loading • When loading samples into the gel, be careful ... pour it into gel forms TIP: If you have a choice of placing the comb in the middle of the gel, you may choose to have students deduce the charge of the molecules from their migration during electrophoresis ... bath until needed Tape the ends of the gel form, if needed, and position in the comb Pour the agar into the gel form; it should come 1/3-1/2 of the way up the comb teeth Let the gel sit undisturbed...
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T-Shirts and Suits A Guide to the Business of Creativity pptx
... Magic of Marketing — This chapter explains that the real meaning of marketing is not about advertising and selling but choosing the right customers in the first place, then being prepared to put them ... not in the sense of putting customers in their sights (as if firing products at them), but putting the customer at the centre of their universe so that their entire business revolves around them ... business model which cuts out most of the costs associated with traditional publishing including printing, warehousing, physical distribution and the remaindering of unsold www.onlineoriginals.com...
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Báo cáo khoa học: Investigating RNA polymerase II carboxyl-terminal domain (CTD) phosphorylation ˆ Benoıt Palancade and Olivier Bensaude pptx
... [75,76,80] The capping enzyme may in turn, assist the recruitment of CDK9 as in S pombe, the CDK9 ortholog binds Pct1, a subunit of the capping apparatus [81] Consistently, inactivation of capping enzyme ... degeneracy of the CTD adds a further level of complexity (Fig 1) Indeed, the N-terminal half of the CTD only stimulates capping whereas the C-terminal half supports capping as well as splicing and ... following inactivation of MAT1, the CDK7 partner [122] These findings are consistent with an H14 staining attributed in part to initiating polymerases (productive or abortive) and an H5 staining of...
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comparison of single and binary oxide moo3, tio2 and wo3 sol – gel gas sensors
... also in uences the gas sensing properties It has been shown that the smaller grain size increases gas sensitivity since the diameter is comparable with or less than the space-charge region of the ... in the grain size, which in addition improves gas sensor response characteristics Current research of binary-metal oxide ®lms is extremely promising but still at an initial stage By varying the ... based structure as the W content of the ®lm increases with respect to the Mo content The gas sensing properties of TiO2, WO3 and MoO3 single metal oxide compounds were examined when exposed to...
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T - engine and T - monitor
... bày thông tin bên cờ kiện đinh event flag ID.Nếu thông số định,thông tin tất cờ kiện trình bày ID Even flag ID WID ID waiting task đầu queue(0 waiting task) PTN Mẫu cờ hành EXINF Thông tin mở rộng ... Free N: Uninit 1: disk-map 2: page-file 4: other map Thông tin map pgf Thông tin page file (PAGEMEM) seg Thông tin quản lý segent spg Thông tin mở rộng OS (KEREXT) pld [01]— Thiết lập Running program ... số định,thông tin tất fixed-size memory pool hiển thị ID Fixed-size memory pool ID WID ID waiting task đầu queue (0 waiting task) FREE Số khối trống EXINF Thông tin mở rộng T-ENGINE / SH7760 –...
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why men don't listening and women don't reading
... range because of their past defending the nest The Slippery Case of the Missing Butter Every woman in the world has had the following conversation with a male who is standing in front of an open ... converted in the brain into a map? Professor Masakazu Konishi of the California Institute of Technology discovered some of the answers using barn owls, birds that are better than humans at pinpointing ... never find a pair of socks but their CDs are in alphabetical order Women can always find the missing set of car keys, but rarely the most direct route to their destination Men think they're the...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25