maintainance and update of molecular biology databases

... filters were 458 nm/band-pass 465-510 nm for CFP, 488 nm/bandpass 500-530 nm for GFP, 514 nm/ bandpass 525-555 nm for YFP, 561 nm/bandpass 575-630 nm for RFP and mCherry, and 488 nm/band-pass 650-710 ... Taipei, Taiwan 3Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 4Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 5Institute of Biotechnology, National ... wavelengths and Hsu et al BMC Plant Biology 2011, 11:60 http://www.biomedcentral.com/1471-2229/11/60 Page 12 of 14 Figure Strategy for identification of BAC clones containing genes of interest Genes of...

Ngày tải lên: 11/08/2014, 11:21

... Department of Obstetrics and Gynecology, Alexandra Hospital, School of Medicine, University of Athens, Greece 2Department of Clinical and Therapeutics, Alexandra Hospital, School of Medicine, ... coordination of all members of the bloodless medicine Page of and surgery team such as surgeons, anaesthesiologists, intensivists, pharmacists, nursing stuff and hematologists The efficient cooperation of ... transfusion Awareness and incorporation of such interventions is mandatory and should be followed by all surgeons and all patients have the right to benefit from the application of these measures...

Ngày tải lên: 13/02/2014, 06:20

... Chloroplast + - Cell organelle Molecular biology: definition • Molecular biology is the study of molecular underpinnings of the process of replication, transcription and translation of the genetic material ... interface of molecular biology and computer science in bioinformatics and computational biology • Since the late 1950s and early 1960s, molecular biologists have learned to characterize, isolate, and ... other areas of biology and chemistry, particularly genetics and biochemistry Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell,...

Ngày tải lên: 13/03/2014, 19:27

... reticulum (ER) synthesis of most lipids synthesis of proteins for distribution to many organelles and plasma membrane Golgi apparatus modification, sorting, and packaging of proteins and lipids for either ... Lesson 1: Basics of molecular biology I The Cell’s Organization II Cell Cycle and Cell Division III Cellular Molecules IV The Genetic Dogma The ... cytosol: the cytoskeleton  “microtubules”  maintainance of cell shape and mobility  ancor for other cellular structures Presentation: DNA Cell Cycle and Cell Division Some Eukaryotic Cell-Cycle...

Ngày tải lên: 13/03/2014, 19:46

... the evaluation of the safety and efficacy of such agents in preclinical models is the identification of suitable candidates for clinical investigation The identification of more TNF and TNFR superfamily ... recent understanding of the role of CDKs in cell cycle regulation and the discovery that approximately 90% of all neoplasia is the result of CDK hyperactivation, leading to the abrogation of the Rb ... past decade the genetic and biochemical analysis of the p53 pathway that leads from cellular stress (through p53 activation) to growth arrest and 256 Impact of Molecular Biology on Cancer Treatment:...

Ngày tải lên: 29/03/2014, 01:20

... 1oAML, and especially in AML secondary to the use of topoisomerase II inhibitors MLL is homologous to the trithorax gene of Drosophila and displays many features of a transcription factor and of ... concentration of sodium chloride are then used to break the ionic interaction between protein and matrix and elute off-bound proteins Examples of such separation media include DEAE and CM cellulose ... function of binding proteins and inhibiting transcription Initiation complex This multi-protein complex forms at the site of transcription initiation and is composed of RNA polymerase, a series of...

Ngày tải lên: 11/04/2014, 07:16

... production of an unspecific band is owing to partial primer duplex formation and polymerization andleads to a product of less than the length of the two primers Landgraf and Wolfes Standard Mismatch ... DNA sequences for gene synthesis, and the specific amplification of limiting quantities of DNA for analysis From Methods m Molecular Biology, Vol 16 Enzymes of Molecular B/o/ogy Edited by M M Burrell ... double-stranded DNA probes (actually only labeled in one or the other of the two strands) by the extension of oligomeric primers of random sequence (8) The oligolabeling technique produces probes of...

Ngày tải lên: 11/04/2014, 09:41

... come out of phage research Conclusion We anticipate that the pages of Virology Journal will reflect the excitement of the "New Phage Biology" by publishing reports in the areas of Ecology and Taxonomy, ... Genomics and Molecular Evolution, Regulation of Gene Expression, Genome Replication and Maintenance, Protein and Nucleic Acid Structure, Virus assembly, Biotechnology, Pathogenesis, Therapeutics and ... divisions of life The large numbers of phylogenetic variants of biologically interesting proteins and nucleic acids that one can derive from sequenced phage genomes are treasure troves for studies of...

Ngày tải lên: 18/06/2014, 22:20

... come out of phage research Conclusion We anticipate that the pages of Virology Journal will reflect the excitement of the "New Phage Biology" by publishing reports in the areas of Ecology and Taxonomy, ... Genomics and Molecular Evolution, Regulation of Gene Expression, Genome Replication and Maintenance, Protein and Nucleic Acid Structure, Virus assembly, Biotechnology, Pathogenesis, Therapeutics and ... divisions of life The large numbers of phylogenetic variants of biologically interesting proteins and nucleic acids that one can derive from sequenced phage genomes are treasure troves for studies of...

Ngày tải lên: 20/06/2014, 04:20

... is Professor of Bioethics and Philosophy of Law, University of Manchester MATTI ¨ HAYRY is Lecturer, University of Iceland, in Privacy Law at the Faculty of Law, and in Computer Security and Cryptography ... providing knowledge of ethical, legal and social aspects of population-based human genetic databases; and (II) to consult citizens in order to gain knowledge of public views of privacy and related moral ... – analysis of the exercise of political, economic and administrative authority in the management of databases; (ii) discourse on databases – analysis of the public discourse on the databases, ...

Ngày tải lên: 05/08/2014, 13:20

... Links, Family Ties and Social Bonds: Rights and Responsibilities in the Face of Genetic Knowledge’, Journal of Medicine and Philosophy 23 (1998), pp 10–30 Cf., however, Tuija Takala and Matti Hayry, ... Obligations and Social Duties’, Journal of Medicine and Philosophy 25 (2000), pp 107–113 20 Matti Hayry and Tuija Takala ¨ Security Genetic databanks need systems and procedures to secure the privacy and ... promotion of the claims of public bodies as the representatives of the ‘people’s voice’; * judicial considerations of public policy – the interest in resolving dilemmas and conflicts of interest...

Ngày tải lên: 05/08/2014, 13:20

... in Iceland that aims at discovering more about Icelanders’ perceptions of privacy of personal information in general and privacy of medical and genetic data in particular, and their trust of private ... a random sample of 1,000 Swedes between eighteen and seventy-five years of age The questions were aimed at mapping attitudes and opinions and did not measure actual knowledge of the field of ... known Little was known also of the public perception of privacy and trust and related moral values in the context of biobanks The purpose of the consultation part of the ELSAGEN project has been...

Ngày tải lên: 05/08/2014, 13:20

... local history and the nationalistic discourse of Icelanders, emphasizing the uniqueness of the Icelandic biological and cultural heritage.17 Weighing risks and benefits In the context of public acceptance ... the University of Tartu, also one of the main founders of the genome project and a board member of the EGP As such, Metspalu appeared as one of the main spokespersons and promoters of the EGP since ... and monitor their current usage.5 Definitions of genetic databases can be found in the laws of Iceland, Sweden and Estonia,6 but these are limited in their scope and may not capture genetic databases...

Ngày tải lên: 05/08/2014, 13:20

... payment of Annual payment of Annual payment of 900,000 EUR 300,000 EUR 200,000 EUR Annual profit payment of Annual profit payment of Annual profit payment 6%, max 900,000 EUR 0.5%, unlimited of 5% ... Conference of UNESCO at its 29th Session on 11 November 1997, art 4, and in the Convention on the Protection of Human Rights and Dignity of the Human Being with Regard to the Application of Biology and ... Committee of Ministers, 1997) Convention on the Protection of Human Rights and Dignity of the Human Being with Regard to the Application of Biology and Medicine: Convention on Human Rights and Biomedicine,...

Ngày tải lên: 05/08/2014, 13:20

... Finally, at a deeper level there are different understandings of ethics and the appropriate role and resourcing of research, and indeed of the concept of global public goods itself Our argument proceeds ... reasons, such as different cultural understandings of disease and genetics, of identity, and different perceptions of the benefits of genetic technologies Benefits and costs: alternative interpretations ... reconsideration of the definition of an orphan medicine, with particular reference to the implications of genetic stratification of both patients and diseases’.21 Here the relevance of the normative and...

Ngày tải lên: 05/08/2014, 13:20

... because of the nature of personal information, because of difficulties with distinguishing adequately between sensitive and nonsensitive information, and because of the nature of computerized databases ... best use of money), governance of the project, aims of the project, scientific value of the project (reliability of the data) and the perception of the public Trust 195 In order to create and maintain ... instance listed in the Book of Icelanders, which is a database containing the genealogical information of 95% of the Icelandic population since the settlement of Iceland over 1,000 years ago, simply...

Ngày tải lên: 05/08/2014, 13:20

... Arnason, Salvor Nordal and Vilhjalmur Arnason ¨ ´ (eds.), Blood and Data: Ethical, Legal and Social Aspects of Human Genetic Databases (Reykjavık: University of Iceland Press and Centre for Ethics, ... negotiation of different 226 Ruth Chadwick and Mark Cutter interests involved requires consideration not only of the rival claims of individuals and communities but also of purposes and benefits, ... this question is to presuppose the elements of competence, disclosure, understanding, voluntariness and consent as definitive of the scope of the concept, and then consider each element in turn In...

Ngày tải lên: 05/08/2014, 13:20

... (eds.), Blood and Data: Ethical, Legal ¨ ´ and Social Aspects of Human Genetic Databases (Reykjavık: University of Iceland Press and ´ Centre for Ethics, 2004), pp 175–179; Sue Weldon and Mairi Levitt, ... interests of a private company that has to follow the rule of the market: profitability In this change rests perhaps one of the most pronounced dangers of today’s science and research: the rise of McScience, ... problem of genetic databases: endangering freedom of an individual in and against the state and society he or she lives in Genetic databases are inherently endangering this freedom because of fundamental...

Ngày tải lên: 05/08/2014, 13:20

... of Chicago Press, 1958 ´ ´ Arnason, G., S Nordal and V Arnason (eds.), Blood and Data: Ethical, Legal and Social Aspects of Human Genetic Databases, Reykjavık: University of Iceland ´ Press and ... Commission, 2003 Council of Europe Explanatory Report to the Convention for the Protection of Human Rights and Dignity of the Human Being with Regard to the Application of Biology and Medicine: Convention ... Implications of the Estonian Genome Project’, Trames (2004), 241–253 Korts, K., S Weldon and M L Gudmundsdottir, ‘Genetic Databases and Public ´ Attitudes: A Comparison of Iceland, Estonia and the...

Ngày tải lên: 05/08/2014, 13:20

... result of a better molecular understanding of a specific cancer mutation she carried The interest in using computational biology to better understand the etiology and pathology of cancer and other ... sites They showed that there is a trade-off between the length of the poly(A) tract and the propor­ tion of adenine nucleotides in it, and that the influence of the tract falls steadily with distance ... study of molecular biology at increasingly fine resolution was another popular theme Jonathan Widom (Northwestern University, Evanston, USA) described the confirmation of previous studies of nucleosome-binding...

Ngày tải lên: 09/08/2014, 20:22