magnetism nmr and epr spectroscopy
Báo cáo khoa học: Defining the QP-site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy doc
Ngày tải lên :
23/03/2014, 13:20
... retains HOQNO binding measured by both the FQ and EPR methods (Figs and 3) Hagerhall and coworkers [20] demonstrated by potenti¨ ¨ ometric titration and EPR spectroscopy that a menasemiquinone radical ... panels, FrdB and FrdA are above the MQ ⁄ HOQNO plane, and FrdC and FrdD are substantially below the MQ ⁄ HOQNO plane Residues from FrdB, FrdC and FrdD have labels starting with ‘B-’, ‘C-’, and ‘D-’, ... is sandwiched between the [3Fe-4S] cluster and the QP site All eight mutant enzymes were studied to assess the effects of the mutations on MQH2 binding using FQ titrations, EPR spectroscopy and...
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Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx
Ngày tải lên :
16/03/2014, 01:20
... the EPR spectra Mobile and immobile components are indicated with arrows (B) Change in activity (d) and C67R1 mobility ( ) with urea concentration The activity in the standard assay and EPR spectra ... structure and dynamics Biochemistry 35, 76927704 Steinhoff HJ (2004) Inter- and intra-molecular distances determined by EPR spectroscopy and site-directed spin labeling reveal protein-protein and protein-oligonucleotide ... structural and dynamical features of a cold-adapted AP using site-directed spin-labeling in conjunction with EPR spectroscopy The effects of cysteine mutations and spin-labeling on kinetic properties and...
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Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
Ngày tải lên :
17/03/2014, 11:20
... Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions J Biomol NMR 2, 661–665 27 Wuthrich, K (1986) NMR of Proteins and Nucleic Acids John Wiley & Sons, New York ... the peptide and secondary structure determination Figure summarizes sequential and medium range NOEs as well as slow-exchanging amide protons for K4L7W and its diastereomer in DPCho and SDS micelles ... peptide and membranes was obtained upon examination of the NMR- calculated average peptide conformations, displaying the relative positions of the two central lysine residues (Lys-5 and Lys-9) and...
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LC-NMR and Other Hyphenated NMR Techniques doc
Ngày tải lên :
05/03/2014, 10:20
... SFE -NMR / 147 6.7 CE -NMR / 147 ix 95 131 143 x CONTENTS 6.8 CEC -NMR / 149 6.9 CZE -NMR / 150 6.10 cITP -NMR / 150 6.11 CapLC -NMR / 152 6.12 SPE -NMR / 154 6.13 SPE-MS -NMR / 159 6.14 Conclusions and ... capillary HPLC (capLC) as capLCNMR and solid-phase extraction (SPE) as SPE -NMR, including SPE hyphenated to MS -NMR as SPE-MS -NMR Many laboratories in industry and academia have NMR as a hyphenated technique ... show the two- and three-bond CÀH correlations for the protons and carbons numbered in the figure and explained in the text The horizontal and vertical traces are the 1D H and 13 C NMR and spectra...
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Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt
Ngày tải lên :
07/03/2014, 16:20
... ionization) and amino-acid analysis [29] NMR spectroscopy Preliminary NMR spectra were acquired at 400 MHz using a Bruker AMX-400 spectrometer (NMR Centre, University of Ioannina, Greece) High-field NMR ... Agonist H2O Lowest energy NMR structure in H2O Lowest energy NMR structure in H2O Lowest energy NMR structure in Me2SO Lowest energy NMR structure in Me2SO Lowest energy NMR structure in Me2SO in ... various systems comprised 3899 and 4516 SPC molecules for the agonist and the antagonist in water, respectively, and 817 and 927 Me2SO molecules for the agonist and the antagonist, respectively,...
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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc
Ngày tải lên :
16/03/2014, 14:20
... assignments of 1H and 13C NMR spectra from a series of disaccharides and tetrasaccharides derived from CS ⁄ DS chains have already been given [16] In this report we present H -NMR and some 13C -NMR data ... Nieduszynski IA, Anderson K, Boocock J, Sandall PL & Weeks SD (2001) Characterization of oligosaccharides from the chondroitin sulfates 1H -NMR and 13C -NMR studies of reduced disaccharides and tetrasaccharides ... concentrator and exchanged several times with 0.5 mL 99.8% 2H2O and then once with 99.96% H2O before final dissolution in 0.7 mL 99.96% 2H2O Preliminary 1H -NMR spectra and all 13C -NMR spectra were...
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Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
Ngày tải lên :
16/03/2014, 14:20
... common and studied CDs are a-, b- and c-CDs, consisting of 6-, 7- and 8-glucosyl units, respectively [14] CDs can be described as toroidal, hollow, truncated cones with a hydrophilic surface and ... drugs, control of volatility and sublimation, physical isolation of incompatible compounds, long-term protection of color, odor and flavor, and suppression of hemolysis and the bitter tastes of drugs ... conditions of pH, buffer and temperature In addition, NMR and DLS analyses demonstrated the characteristic properties of GA Our results of the molecular interaction between GA and c-CD can be correlated...
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Ir and raman spectroscopy wartewig
Ngày tải lên :
17/03/2014, 14:46
... of IR and Raman spectroscopy in various scientific disciplines ranging from chemistry and physics to bioscience and medicine On the other hand, handling, manipulating and evaluating of IR and Raman ... Siegfried Wartewig IR and Raman Spectroscopy Fundamental Processing 2003, ISBN 3-527-30245-X Christian Schorn NMR Spectroscopy: Data Acquisition 2001, ISBN 3-527-28827-9 Peter Bigler NMR Spectroscopy: ... appears at ~L 9394 cm–1 and the bands at wavenumbers lower and higher than n 9394 cm–1 arise from Stokes and anti-Stokes Raman scattering, respectively On the other hand, a standard Raman spectrum...
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Báo cáo khoa học: NMR and MS evidences for a random assembled O-specific chain structure in the LPS of the bacterium Xanthomonas campestris pv. Vitians A case of unsystematic biosynthetic polymerization potx
Ngày tải lên :
31/03/2014, 09:20
... by standard procedure [7] and monitored by GLC-MS of the partially methylated alditol acetates NMR spectroscopy Chemical shifts obtained by NMR spectroscopy were assigned using 2D homo- and heteronuclear ... injected from a sample solution earlier analyzed with NMR and five fractions were manually collected and freeze-dried MALDI-TOF MS Fractions and from partial hydrolysis of the O-specific polysaccharide ... yielded the lipid A moiety as precipitate and the OPS was isolated from the supernatant and further purified by gel-permeation chromatography H and 13C NMR spectra of the OPS were indicative of...
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Báo cáo hóa học: " FTIR and Raman Spectroscopy of Carbon Nanoparticles in SiO2, ZnO and NiO Matrices" pdf
Ngày tải lên :
22/06/2014, 01:20
... absorption band, at 900 cm-1, is an absorption band that can be assigned to the stretching vibration of Si–OH or Si–O- groups A broad absorption band, situated between 3,000 and 3,600 cm-1, and another ... are weaker than the G peaks and this suggests that the grain sizes could be larger than 44 nm [12] From the peak positions xD and xG and intensities ID and IG of the D and G-peaks, respectively, ... ultraviolet and visible wavelength ranges (0.3–2.5 lm) Raman spectroscopy was also used to study these samples using a Jobin-Y von T64000 Raman spectrometer The observations from the FTIR and Raman spectroscopy...
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Rotational and Vibrational Spectroscopy pdf
Ngày tải lên :
27/06/2014, 01:20
... crystalline phonon bands P F Taday and D A Newnham, Spectroscopy Europe, , www.spectroscopyeurope.com G Winnewisser, Vibrational Spectroscopy (1995) 241-253 23 Applications of Near IR Spectroscopy ... Near-infrared, In Handbook of Vibrational Spectroscopy, Vol 3, Wiley, U.K., pp 1817-1837 Workman, J (2000) Handbook of Organic Compounds: NIR, IR, Raman, and UV-Vis Spectra Featuring Polymers and Surfactants, ... PA-FTIR spectrum and a regular IR spectrum: – IR modulation frequency effects (weak CH3 and CH2 bands) – Saturation of strong bands in the spectrum Paroli, R M., Delgado, A H., and Cole, K C Canadian...
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IR and Raman Spectroscopy Fundamental Processing docx
Ngày tải lên :
29/06/2014, 06:20
... of IR and Raman spectroscopy in various scientific disciplines ranging from chemistry and physics to bioscience and medicine On the other hand, handling, manipulating and evaluating of IR and Raman ... Siegfried Wartewig IR and Raman Spectroscopy Fundamental Processing 2003, ISBN 3-527-30245-X Christian Schorn NMR Spectroscopy: Data Acquisition 2001, ISBN 3-527-28827-9 Peter Bigler NMR Spectroscopy: ... appears at ~L 9394 cm–1 and the bands at wavenumbers lower and higher than n 9394 cm–1 arise from Stokes and anti-Stokes Raman scattering, respectively On the other hand, a standard Raman spectrum...
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Báo cáo y học: " Homonuclear 1H NMR and circular dichroism study of the HIV-1 Tat Eli variant" doc
Ngày tải lên :
13/08/2014, 05:21
... Phe, Ser, Tyr and Trp; 14/14 Gln, Glu and Met; 9/9 Gly, 2/2 Ala, 16/16 Pro, 16/18 Arg and Lys, 2/2 Ile, 7/7 Val and Leu, and 3/3 Thr The homonuclear 1H NMR spectra of Tat Eli allowed sequential ... dichroism and NMR studies and was involved in drafting and revising the manuscript, GRC helped synthesize Tat proteins, participated in the design of the study, carried out trans-activation assays and ... synthesis and has biological activity as demonstrated in a trans-activation assay Circular dichroism (CD) experiments indicate that Tat Eli is not a random coil at 20°C 2D NMR spectra of Tat Eli and...
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nanotechnology and chiroptical spectroscopy to characterise optically active chiral metamaterials
Ngày tải lên :
22/12/2014, 19:52
... 4: SHG spectra for right handed gammadions(R-gammadions (red)) and left handed gammadions (L-gammadions (blue)) as well as for right handed G`s (R-G`s (red)) and left handed G`s (L-G`s (blue)) ... via CD spectroscopy and scanning microscopy 117 3.1 Introduction 117 3.1.1 Chirality and biomolecules sensing 117 3.1.2 CD spectroscopy 118 3.2 Theory and background ... right handed gammadions (R-gammadions (red)) and left handed gammadions (L-gammadions (blue)) as well as for right handed G`s (R-G`s (red)) and left handed G`s (L-G`s (blue)) From this Figure,...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
Ngày tải lên :
19/02/2014, 12:20
... Conformational studies on the selectin and natural killer cell receptor ligands sulfo- and sialyl-lacto-N-fucopentaoses (SuLNFPII and SLNFPII) using NMR spectroscopy and molecular dynamics simulations ... pattern, sequence, and partial linkages, and assign structural details of each branch (e.g the 3- and 6-branches) Blood group H, Lea/Lex and Leb/Ley determinants, together with type and type chains, ... the iso-lactoN-octaose core, and fully characterized four structures by the combined use of ES-MS, methylation analysis and NMR spectroscopy The branching pattern and blood group-related Lewis...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
Ngày tải lên :
20/02/2014, 02:22
... conformational changes of the N and B states of Mn(III)heme–HSA, by optical absorbance spectroscopy and NMR spectroscopy, that show allosteric interaction(s) between FAs and Mn(III)heme with HSA Interestingly, ... transport of lipophilic compounds and drugs and of medium and long chain fatty acids; among them, myristic acid is a stereotypic ligand to investigate fatty acid binding and transport properties of ... propionates point toward the interface between domains I and III and are stabilized by salt bridges with His146 and Lys190 residues [6,8] HSA undergoes pH- and allosteric effector-dependent reversible conformational...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
Ngày tải lên :
17/03/2014, 10:20
... sequencing In both its E- and Z-configurations, peptide was characterized further by NMR spectroscopy peptide charges because bands with nearly the same charge (bands 1B, 1C and 2) migrated quite ... the intact a-PEC and the derived peptides The excellent solubility of peptides and at pH 7.0 recommended their employment for further studies such as crystallization and NMR spectroscopy Unfortunately, ... occurred during storage in formic acid The Z- and E-configurations of one chromopeptide with an excellent solubility and stability were investigated by 1D NMR spectroscopy The information presented here...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
Ngày tải lên :
30/03/2014, 20:20
... characteristics of EM2 and EM2OH in DMSO, TFE, H2O (pH 2.7 and 5.2) and DPC micelles (pH 3.5 and 5.2) Open and fold represent the conformations rS and n7 in parentheses indicate the reverse S- and numerical ... (pH 2.7 and 5.2) and DPC micelles (pH 3.5 and 5.2), and in monocationic form in water (pH 2.7) A typical difference between the EM2 and EM2OH was observed for the pH dependence of their NMR spectra ... (pHs 2.7 and 5.2), and DPC micelles (pHs 3.5 and 5.2) Table S2 Interresidual ROE connectivities along the peptide backbones in TFE, water (pHs 2.7 and 5.2), and DPC micelles (pHs 3.5 and 5.2)...
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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
Ngày tải lên :
31/03/2014, 21:21
... structures were examined using CD spectroscopy and 2D homonuclear/heteronuclear NMR spectroscopy Using these peptides, the interaction between the amphipathic domain of YopD and its cognate chaperone ... no residues were in disallowed regions, Ramachandran plot analysis was conducted using the program PROCHECK -NMR [37] RESULTS AND DISCUSSION CD and NMR studies Computer analysis of YopD primary ... for further NMR analysis A temperature of 40 °C was chosen in order to minimize peptide aggregation and obtain a better resolved spectrum All NMR spectra were recorded on a Bruker DRX and a Bruker...
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Probing protein dynamics and protein protein interaction by NMR spectroscopy
Ngày tải lên :
11/09/2015, 10:17
... (Fields and Song 1989), affinity purification with MS/MS identification (Aebersold and Mann 2003), methods based on fluorescence technologies (Yan and Marriott 2004) and a variety of NMR spectroscopy ... Figure 2-1 Sequence and secondary structure of HIP2 (PDB Code: 1YLA) Helices, sheets and turns are colored in red, blue and green, respectively 34 2.2 Materials and Methods 2.2.1 NMR Spectroscopy 2.2.1.1 ... decay (FID) and demonstrated the improvement in signal to noise ratio over the continuous wave method (Ernst and Anderson 1966), which brought a new era of pulsed Fourier Transform NMR spectroscopy...
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