magic angle spinning solid state nmr experiments for structural characterization of proteins
nmr methods for the investigation of structure and transport
Ngày tải lên :
29/05/2014, 18:32
... organized the continuation of the research unit after the DFG funding as well as Prof Kasper, Prof Kind, Prof Nirschl, and Prof Elsner Prof Nirschl, Prof Kind, Prof Wilhelm, and Prof Elsner contributed ... transform of raw data and the continuous function of interest is formulated The influence of gradient imperfections on velocity measurements is assessed This is of particular importance for experiments ... points in ˛ direction Number of averages Number of nuclear spins Set of indexes (first index) or number of slices Set of indexes (second index) Population probability for state m Normal distribution...
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Báo cáo y học: "Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pptx
Ngày tải lên :
11/08/2014, 05:21
... This means that each of the profiles was combined with its 'foldover' profile A foldover profile includes the exact opposite attribute levels of the original profile and, therefore, ensures a completely ... users of the results and responsible for getting the research findings into practice Therefore, we asked respondents for their opinions about the feasibility of DCE In particular, we asked for ... elsewhere [8] Therefore, we will now present the DCE method to the extent that the information is relevant for understanding of the present paper For more theoretical background information about...
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báo cáo khoa học: " Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pot
Ngày tải lên :
11/08/2014, 16:21
... This means that each of the profiles was combined with its 'foldover' profile A foldover profile includes the exact opposite attribute levels of the original profile and, therefore, ensures a completely ... users of the results and responsible for getting the research findings into practice Therefore, we asked respondents for their opinions about the feasibility of DCE In particular, we asked for ... elsewhere [8] Therefore, we will now present the DCE method to the extent that the information is relevant for understanding of the present paper For more theoretical background information about...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
Ngày tải lên :
18/02/2014, 06:20
... flexibility of the NMR structure of micelle-associated HIV-1 VpUcyt amino acid stretch 64–78 is also evident from the heteronuclear NOE values observed for this region (Fig 8B) Earlier solid state NMR ... topic and may be studied by solid state NMR methods However, the objective of the present study was to gather structural and dynamic data of VpUcyt in the presence of membrane-simulating DPC micelles ... by solid- state NMR with magic- angle spinning Biochemistry 45, 918–933 Henklein P, Schubert U, Kunert O, Klabunde S, Wray V, Kloppel KD, Kiess M, Portsmann T & Schomburg D (1993) Synthesis and characterization...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
Ngày tải lên :
18/02/2014, 16:20
... COFRADIC procedure was applied to a trypsin digest of 10 lL of mouse serum depleted for its three most abundant proteins (albumin, IgGs and transferrin), and resulted in the characterization of ... of ATP-binding proteins As expected, the majority of FSBA-labeled Jurkat proteins were known binders of small nucleotides, cofactors, or DNA and RNA molecules However, several proteins and sites ... COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ) protease degradomics...
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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx
Ngày tải lên :
22/02/2014, 08:20
... mechanical characterization of protein materials may allow for gaining insight into the biological functions of mechanical proteins Mechanical characterization of biological molecules such as proteins ... degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif For deeper understanding the role of native ... Abstract Mechanical characterization of protein molecules has played a role on gaining insight into the biological functions of proteins, since some proteins perform the mechanical function...
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Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt
Ngày tải lên :
31/03/2014, 07:20
... approaches offer generic advantages for the analysis of protein complexes both in terms of dynamic range (i.e how little of one protein can be identified in the presence of how much of a mixture of other ... 571 Analysis of protein complexes Within the scope of this review, we will focus on summarizing the current technical state of the art for the biochemical retrieval of associated proteins and ... large excess of contaminating proteins (e.g antibodies in IP experiments) Conclusions The combination of affinity methods for the purification of protein complexes and the identification of their components...
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Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx
Ngày tải lên :
18/06/2014, 19:20
... analysis of CTCs derived from tumors with down-regulation of surface epithelial markers such as EpCAM For this reason, depletion of the leukocyte fraction (CD45 depletion) for enrichment of CTCs ... protocol was applied for detection of CTCs in a cohort of patients with metastatic carcinomas or melanoma Materials and methods Comparison of three different enrichment methods Spiking Experiments The ... by incubating the cells in 100 μL PBS for 10’ at 4°C Cells were then washed with PBS, and fixed with 1% formaldehyde for 20’ at 4°C before permeabilization for intracellular staining To permeabilize...
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báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx
Ngày tải lên :
20/06/2014, 03:20
... analysis of CTCs derived from tumors with down-regulation of surface epithelial markers such as EpCAM For this reason, depletion of the leukocyte fraction (CD45 depletion) for enrichment of CTCs ... protocol was applied for detection of CTCs in a cohort of patients with metastatic carcinomas or melanoma Materials and methods Comparison of three different enrichment methods Spiking Experiments The ... by incubating the cells in 100 μL PBS for 10’ at 4°C Cells were then washed with PBS, and fixed with 1% formaldehyde for 20’ at 4°C before permeabilization for intracellular staining To permeabilize...
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báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx
Ngày tải lên :
11/08/2014, 00:22
... conjugate formation Bioconjugation of proteins to carboxylated QDs have been performed with the use of EDC alone [7] Despite the simplicity of a one-step reaction, the drawback involves a degree of ... proteins This ultimately Another drawback for the use of EDCHCl/sulfo-NHS for the formation of stable bioconjugates is the lack of specificity on the protein of interest As numerous amine functional ... derivatization of QD-COOH with EDC/ADH; and c) conjugation of QD-hydrazide with IgG-CHO via formation of hydrazone linkage to yield QD-IgG [34] Their work followed the characterization of the conjugates'...
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báo cáo khoa học: " A new model for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation" pptx
Ngày tải lên :
11/08/2014, 20:21
... direction of fire The tests were performed with gelatin blocks (n = 12) They consisted of 20% porcine gelatin (Merck, Darmstadt, Germany), and water and had an edge length of 12 × 12 × 18 cm Page of ... examination of the infiltration depth of barium titanate particles within the ruptures of a temporary cavity in the gelatin block revealed a deposition of particles along the entire bullet path for both ... both types of projectiles examined In the case of the soft point projectile, there were no significant differences between the size of the temporary cavity and the infiltration depth of the barium...
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Báo cáo y học: "Novel multiplex technology for diagnostic characterization of rheumatoid arthritis" pdf
Ngày tải lên :
12/08/2014, 17:22
... of antiCCP-antibodies or RF) and genetic (possession of SE alleles) parameters predictive of more severe RA Unfortunately, information on the degree of radiographic joint damage at the time of ... Results Analytical precision of IMPACT assays To develop a system for the multiplex analysis of different types of biomarkers in the sera of RA patients, we Page of 13 used a bead-based commercial ... achieved with the IMPACT platform, we performed 21 replicate measurements of each of nine markers within one run on the IMPACT platform The intra-assay coefficients of variance (CV) ranged from...
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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE
Ngày tải lên :
24/08/2014, 09:51
... represent the average of three independent sets of experiments with each set of experiments performed twice D Structural classification of compounds A series of compounds that independently emerged from ... identify potential inhibitors of ALDH3A1 29 D Structural classification of compounds 31 viii E Steady state kinetic characterization 31 F Search for structurally related analogs ... 110 A Characterization of CB29 binding .113 B Selectivity of CB29 for ALDH3A1 versus ALDH1A1 and ALDH2 117 C Characterization of CB7 binding 123 D Probing CB7 binding of ALDH3A1...
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guide for structural maintenance of parking structures
Ngày tải lên :
24/10/2014, 21:59
... types of structural systems can develop different types of deterioration-related problems ACI 362.1R contains discussion of durability considerations for parking structures An understanding of these ... variety of tests performed The most important tests are those that determine the extent of corrosion and bond loss of the reinforcement and those that quantify the amount and extent of chloride ... 3.2.3 for evaluation methods) Check for and evaluate cracks § AR A Check for leaks Grind out and fill with sealant Determine if structural or nonstructural Check joint sealants *† AR A Review for...
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Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films
Ngày tải lên :
10/09/2015, 08:41
... SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MECHANICAL ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Preface This dissertation is submitted for the degree of Doctor of Philosophy ... in the Department of Mechanical Engineering, National University of Singapore (NUS) under the supervision of Associate Professor Zeng Kaiyang To the best of my knowledge, all of the results presented ... Piezoresponse Force Microscopy 18 2.1 Working Principle of Piezoresponse Force Microscopy 18 2.1.1 Experimental Setup 2.2 Theory of PFM 18 21 2.2.1 Capacitive forces 23 2.2.2 Electromechanical Forces...
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Structural characterization of nogo proteins implications for biological functions and moleculedesign of therapeutic applications
Ngày tải lên :
14/09/2015, 12:53
... 53 3.1 Structural Characterization of Inhibitory Domains of Nogo 54 3.1.1 Expression and structural characterization of NogoA-(567-748) and Nogo-66 55 3.1.2 CD and NMR characterization of Nogo-40 ... 3.3.4 CD and NMR characterization of the Nogo-B N-terminus 99 Figure 3.3.5 CD and NMR characterization of the Nogo-33 101 Figure 3.3.6 CD and NMR characterization of the first group of the dissected ... 3.6 Structural Characterization of NgBR 134 3.6.1 Cloning and expression of NgBR and its dissected domains 136 3.6.2 Bioinformatics characterization 138 3.6.3 Structural characterization of the...
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Tài liệu ComProtect® Solid-State Single-Pair Protectors for Series 2 Switching Blocks docx
Ngày tải lên :
10/12/2013, 18:15
... ComProtect® Solid- State Single-Pair Protectors for Series Switching Blocks Features: • Meets applicable GR-974-CORE, GR-78-CORE and GR-63-CORE standards • Meets UL 497 standard for paired conductor ... aging Also meets standards for environmental cycling for impulse and AC surges • Meets GR-63 standards for transport and storage • NEBS Level compliant • Insertion loss of less than 0.6 dB (up to ... • Cabinet overlay applications Compact 307 type replacement for protecting inputs and outputs of DSLAM equipment 7/07 • 1 A E Solid- State ComProtect® • Both 24- and 26-gauge wire can be used...
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Fundamentals of quantum mechanics for solid state electronics, optics c tang
Ngày tải lên :
17/03/2014, 14:41
... the time-dependence of the state of the system The mathematical solution of the equation of motion for the dynamic variables of the system will then tell us precisely the state of the system at ... abstract state function that contains all the information about the state of the dynamic system For this statement to have meaning, we must obviously provide a clear physical interpretation of the state ... later The state function is a function of a set of chosen variables, called ‘‘canonic variables,’’ of the system under study For definiteness, let us consider again, for example, the case of a particle...
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