luminescent quantum dots as advanced biological labels

... applications as fluorescent biological labels Human embryonal kidney cell line is chosen as typical kind of human cells to demonstrate the promising applications as fluorescent biological labels MTT assays ... decreases not only the monochromaticity of the fluorescence but also the quantum yields of the QDs In our case, the quantum yields of the as- synthesized CdSe cores are around 2–3% In order to passivate ... formazen product was then measured at a wavelength of 570 nm Four groups of MTT tests were done for each quantum dots concentration The values of MTT assay of labeled cells were expressed as the Page...

Ngày tải lên: 21/06/2014, 08:20

... the increased upatke was related to FA, a competition assay was performed with free FA In the case of QD (C) internalisation was reduced by free FA to the same level as QD (A) alone As expected ... three flasks, A, B and C Folic acid (0.01 moles, 0.28 g) was added directly to Flask B and the solution was stirred for 15 EDC (0.1 g) and DMAP (0.1 g) were added to flask C and the solution was stirred ... membrane Its fluorenscence is only released upon action of esterases in the cytoplasm Since only viable cells produce active esterases, it can be used to assess cytotoxicity[18] Thiol-stabilised...

Ngày tải lên: 11/08/2014, 08:20

... proliferation of fibroblasts was evaluated by two different methods, BrdU assay and morphological observation Figure shows the pattern of fibroblast proliferation as measured by BrdU assay after and ... Semiconductor nanocrystals as fluorescent biological labels Science 1998, 28:2013 Page of 11 Chan WCW, Maxwell DJ, Gao X, Bailey RE, Han M, Nie S: Luminescent quantum dots for multiplexed biological detection ... fibroblasts cultured in the absence and presence of CSNPs, PCSNPs, and ICSNPs was determined by colorimetric immunoassay based on the measurement of 5-bromo-2-deoxyuridine (BrdU), which was incorporated...

Ngày tải lên: 20/06/2014, 22:20

... selfordered InGaAs quantum dots Appl Phys Lett 2005, 87:213105 Kiravittaya S, Songmuang R, Rastelli A, Heidemeyer H, Schmidt OG: Multiscale ordering of self-assembled InAs/GaAs(001) quantum dots Nanoscale ... Self-organization of (In, Ga )As/ GaAs quantum dots on relaxed (In, Ga )As films Appl Phys Lett 1998, 73:2164-2166 Kim KM, Park YJ, Park YM, Hyon CK, Kim EK, Park JH: Alignment of InAs quantum dots on a controllable ... of InGaAs/GaAs quantum dots J Cryst Growth 1998, 195:540-545 Leon R, Kim Y, Jagadish C, Gal M, Zou J, Cockayne DJH: Effects of interdiffusion on the luminescence of InGaAs/GaAs quantum dots Appl...

Ngày tải lên: 21/06/2014, 01:20

... References Zh M Wang, Self-Assembled Quantum Dots (Springer, New York, 2008); E.Y Tsymbal, O Mryasov, P.R LeClair, J Phys.: ˇ ´ Condens Matter 15, R109 (2003); I Zutic, J Fabian, S Das Sarma, Rev Mod ... RSOI-induced phase factor, and then the QDs in the present system can be either a gated or a self-assembly one, making it easier to be realized in current experiments Acknowledgment This work was supported ... Then the spin-up conductance G" has a finite value but meanwhile G# ¼ as the conduction channel for the spin-down electrons breaks off, which is shown by the dot-dashed lines in Fig 2a, b The spin...

Ngày tải lên: 22/06/2014, 01:20

... synthesis were purchased from Sigma-Aldrich Al2Te3 was purchased from Cerac Inc AlamarBlue was purchased from Biosource International A BrdU cell proliferation kit was purchased from Roche Diagnostics ... Howarth M, Takao K, Hayashi Y, Ting AY: Targeting quantum dots to surface proteins in living cells with biotin ligase PNAS 2005, 102:7583-7588 30 Sundara Rajan S, Vu TQ: Quantum Dots Monitor TrkA ... acquisition was also used to acquire three colour images Rhodamine phalloidin was excited using the HeNe1 543 nm laser and the emitted fluorescence was acquired at 575 nm DAPI stain was excited with laser...

Ngày tải lên: 11/08/2014, 00:22

... 6(2), (2006) Multicolor quantum dots for molecular diagnostics www future-drugs.com 1.2 Time (min) quantum dots 15 10 20 25 30 35 Intensity (au) Quantum dots 0.8 0.6 0.4 0.2 Texas red 0 20 40 60 80 ... nanoparticle that has the potential to be used as a sensitive probe for screening cancer markers in fluids, as a specific label for classifying tissue biopsies, and as a high-resolution contrast agent ... accurately assess invasive potential of different cancer cell types, as motility of cells is strongly associated with their malignancy in vivo This new assay could aid in the clinical classification...

Ngày tải lên: 15/02/2014, 05:20

... curve and measured absorption spectra, we can estimate the mean size of QDs Based on the analysis it has been expressed the experimental formula to estimate the mean sizes of QDs CdSe as: (2) D ... analysis revealed no impurities such as Se and SeO3 in the sample As expected, the width of the diffraction peaks is considerably broadened and can be determined easily because the size effect is ... Transmission electron microscopy (TEM) was carried out using a microscope Ultraviolet–visible (UV–vis) absorption spectra of the nanoparticles were recorded by using a Jasco V670 spectrophotometer Result...

Ngày tải lên: 14/03/2014, 13:20

... biomass is washed out or wasted from the system faster than it can be reproduced (7) It seems from Fig 1.4 that when washout occurs, the influent waste concentration (So ) should equal the waste ... 9, Advanced Biological Treatment Processes, is a sister book to Volume Biological Treatment Processes Both books have been designed to serve as comprehensive biological treatment textbooks as ... Principles and Kinetics of Biological Processes waste concentration may be risky in case it could not support a sustainable and viable biomass needed for biological treatment As Eq (3) indicates, the...

Ngày tải lên: 01/04/2014, 10:17

... are used as illustrations of the basic theory The đrst two chapters serve as an introduction to quantum theory, although it is assumed that the reader has been exposed to elementary quantum mechanics ... wave packet in equation (1.20) As time increases from to I, the half width continuously increases and the maximum amplitude continuously decreases Thus, as t increases, the wave packet jỉó (x, ... function Quantum mechanics is a theory to explain and predict the behavior of particles such as electrons, protons, neutrons, atomic nuclei, atoms, and molecules, as well as the photonthe particle associated...

Ngày tải lên: 24/04/2014, 17:11

... organic passivated quantum dots, inorganic layer passivated quantum dots have some merits Uniform coating could be coated on the surface of the quantum dots in order to accomplish high quantum ... or sputtering Quantum dots can be self assembled on a thin film by chemical vapor deposition (CVD) 1.4 Quantum Dots Biological Applications Quantum dots are small, compared with biological tissues, ... H2Te gas 14 The chemical affinity of CA on the CdTe quantum dots is not as good as TGA Therefore, after the H2Te gas introduction, small lumps of quantum dots agglomeration appeared In that case,...

Ngày tải lên: 28/04/2014, 15:48

... direct-bandgap semiconductor materials, such as GaSb/GaAs [8-10], InAlAs/InP [11], InP/InGaP [12, 13], InP/GaAs [14], GaAsSb/GaAs [15], and InAs/GaSb [16, 17] The reason is that they offer comparatively ... in type-II GaSb/GaAs quantum dots Appl Phys Lett 1995, 67:656 10 Alonso-Álvarez D, Alén B, García JM, Ripalda JM: Optical investigation of type II GaSb/GaAs self-assembled quantum dots Appl Phys ... properties of InAsP ternary self-assembled quantum dots embedded in GaAs Appl Phys Lett 2002, 81:2953 15 Liu G, Chuang S-L, Park S-H: Optical gain of strained GaAsSb/GaAs quantum- well laser: a self-consistent...

Ngày tải lên: 20/06/2014, 20:20

... Ph, 3) as colorless compounds in 30% to 70% isolated yield as shown in Scheme Compounds and were isolated as colorless oil; however, compound was obtained as a colorless solid Compound was pyrophoric ... alloy was formed At an ambient temperature, 50 ml of dimethoxyethane and red phosphorus (0.22 g, 7.0 mmol) were added to a flask containing the sodium/potassium alloy The reaction mixture was slowly ... which 15 ml of THF was added The colorless solution was filtered, and the solvents evaporated to dryness to afford as a white solid (0.8 g, 30.8 %) The boiling point of was observed at 108°C...

Ngày tải lên: 20/06/2014, 20:20

... semiconductor quantum dots (SQDs) in an SQD-MNP coupled system We propose a quantum transformation method to strongly reveal the exciton energy shift and the modified decay rate of SQD as well as the ... 2010, 4:6369 Manjavacas A, Abajo FJG, Nordlander P: Quantum plexcitonics: strongly interacting plasmons and excitons Nano lett 2011, 11:2318 Passmore BS, Adams DC, Ribaudo T, Wasserman D, Lyon S, ... present article, cavity QED as a quantum optics toolbox provides a full quantum mechanics description of the coupled SQD-MNP system Under the description we develop a novel quantum transformation...

Ngày tải lên: 20/06/2014, 20:20

... concept for strainbalanced (In, Ga )As/ Ga (As, P) quantum dot solar cells Phys Rev B 2008, 98:205321 Luque A, Marti A, Nozik J: Solar cells based on quantum dots: multiple exciton generation and ... has been studied by employing photoluminescence [PL] measurements PL in QD solar cells was measured under short-circuit conditions To stimulate PL, we used the 514-nm line from an Argon-ion laser ... were measured by an InGaAs detector array In Figure 8, we compare the PL from p- and n-doped samples (four carriers per dot) at 1- and 4-W/cm2 intensities As seen, p-doping drastically increases...

Ngày tải lên: 20/06/2014, 22:20

... reaction was implemented in each assay as negative control, and each sample was performed in triplicate PCR products were analyzed in 1% agarose gel electrophoresis Characterization of quantum dots ... to the data, the cutoff value of this assay was defined as 14.5, any result under 14.5 from the 160 DNA samples was considered as positive one (Figure 3) Based on this cutoff value, all of the ... NaBH was added into a 100mL flask with 50 mL of Milli-Q water The reaction was implemented in room temperature with N2 protection and lasted until the Tellurium powder disappeared in the flask...

Ngày tải lên: 21/06/2014, 01:20

... absorption peak, which was also used for the excitation of the QD samples in the PL measurement The molar ratio of [Cd2+]/[MPA]/[HSe-] was set as 1:2.4:1, the concentration of Cd2+ was fixed at 1.25 ... of their first excitation absorption peak As shown in Figure 2b, an increase of the decay lifetime is observed with the increase of the CdSe shell It was reported that the wave function overlap ... PL intensity gradually decreased with the decreasing absorption value, which shows that fast coating will lead to a larger amount of surface defects and thus decrease of PL intensity In our experiment,...

Ngày tải lên: 21/06/2014, 03:20

... broadening in single selfassembled InAs/GaAs quantum dots Phys Rev B 2001, 65:033313 13 Vasanelli A, Ferreira R, Bastard G: Continuous Absorption Background and Decoherence in Quantum Dots Phys Rev Letts ... Kammerer C, Cassabois G, Voisin C, Delalande C, Roussignal Ph: Photoluminescence Up-Conversion in Single Self-Assembled InAs/GaAs Quantum Dots Phys Rev Letts 2001, 87:207401 Finley JJ, Ashmore AD, ... quantum dots: An ab initio perspective Chem Phys Letts 2008, 460:1-9 Toda Y, Moriwaki O, Nishioka M, Arakawa Y: Efficient Carrier Relaxation Mechanism in InGaAs/GaAs Self-Assembled Quantum Dots Based...

Ngày tải lên: 21/06/2014, 03:20

... QDs + DNR treatment induces cytochrome c release, causing caspase-9 activation Cleaved caspase-9 activated caspase-3 that correlated with the increased expression of cleaved PARP after relevant ... (lane 4) The following antibodies were used: anti-cleaved caspase9, anti-cleaved caspase-3, and anti-PARP antibody GAPDH was served as a loading control Figure Inhibition of tumor growth in HepG2/ADM ... released into the cytosol and significantly activated caspase-9 and caspase-3 and induced degradation of its substrates, PARP These data suggest that Cdte QDs with DNR treatment involve the release...

Ngày tải lên: 21/06/2014, 03:20

... in one-dimesional quantum dots Phys Lett A 2005, 335:175 Baskoutas S, Paspalakis E, Terzis AF: Effects of excitons in nonlinear optical rectification in semi-parabolic quantum dots Phys Rev B 2006, ... Second-harmonic generation resonant with s-p transition in InAs/ GaAs self-assembled quantum dots Phys Rev B 2001, 63:113312 11 Que W: Excitons in quantum dots with parabolic confinement Phys Rev B 1992, ... magnitude as can be seen in Figure Because of this difference, several authors [5,6,8] used the strong-confinement limit as a satisfactory approximation in the case of small quantum dots Accordingly...

Ngày tải lên: 21/06/2014, 04:20