loss infection and dysfunction of dendritic cells in hiv infection
Báo cáo khoa học: " Hematology, cytochemistry and ultrastructure of blood cells in fishing cat (Felis viverrina)" ppt
Ngày tải lên :
07/08/2014, 20:23
... yltniaf dna elbinrecsidni tsomla htiw suelcun detcirtsnoc dah yehT )1 elbaT( setycokuel tnelaverp tsom eht erew slihportueN slihportueN sgniniats lacimehcotyc lla rof evitagen erew yehT dnuof netfo ... tiknurihirP kasgnaerK 461 .ylevitcepser ,SAP dna ulg-β rof evitisop ylkaew )G & F( EANA rof evitisop ylgnorts )E( ylevitcepser ,BBS dna REP rof evitagen )D & C( ylevitcepser ,W dna GW ,selunarg ... ,selunarg epahs-dor htiw lihponisoe na )B & A( :slihponisoE SAP rof evitisop ylgnorts )G( ulg-β rof deniats ylevitisop )F( EANA rof evitisop ylkaew )E( ylevitcepser ,BBS dna REP ,slihportuen evitisop...
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Báo cáo y học: "Abnormal costimulatory phenotype and function of dendritic cells before and after the onset of severe murine lupus" pot
Ngày tải lên :
09/08/2014, 07:20
... AE: Differences in expression of lupus nephritis in New Zealand mixed H-2z homozygous inbred strains of mice derived from New Zealand black and New Zealand white mice Origins and initial characterization ... profile found in DCs from patients with SLE [2,4] and validates the use of NZM2410 and NZB-W/F1 strains as murine models for the study of human SLE CD80 and CD86 are ligands of CD28/CTLA4 and ... mice and their single-locus derivative B6.Sle3 mice have a genetic abnormality, still to be determined and not intrinsic to DCs, that induces upregulation of CD40 in DCs in vivo before and after...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
Ngày tải lên :
12/08/2014, 13:22
... Generation and Differentiation of Th17 Cells and Tregs in MPE Purified naïve CD4+ T cells (5 × 105) were cultured in ml of complete medium containing human IL-2 (2 ng/ ml) in 48-well plates and stimulated ... and Th17 Cells in MPE We determined some cytokines that reported be involved in generation and differentiation of Tregs or Th17 cells, and observed that high levels of IL-1b, IL-6, and TGF-b1, ... naïve CD4+ T cells after culturing in presence both IL-1b and IL-6 (C) The representative dot plots of CD39+Tregs and Th17 cells detected in naïve CD4+ T cells after culturing in presence of TGF-b1...
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Báo cáo y học: " Epratuzumab targeting of CD22 affects adhesion molecule expression and migration of B-cells in systemic lupus erythematosus" potx
Ngày tải lên :
12/08/2014, 15:21
... expression of a4b7 integrin is regulated in a homeostatic relation with the surface expression of a4b1 integrin on T -cells since high expression of a4b1 integrin resulted in a loss of a4b7 integrin With ... tyrosine kinase) which is involved in BCR signaling, but also in the regulation of integrin activation [44] Moreover, recent work indicates that Syk is involved in CXCL12- and a4b1 integrin-induced ... the b1 integrin on the surface of B -cells In this regard, integrin complexes are composed of b and a subunits and form unique molecules, and b7 integrin essentially competes with b1 integrin for...
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Báo cáo y học: " Effectiveness of antiretroviral therapy and development of drug resistance in HIV-1 infected patients in Mombasa, Kenya" doc
Ngày tải lên :
10/08/2014, 05:21
... Prevalence and risk factors for developing K65R mutations among HIV- 1 infected patients who fail an initial regimen of fixed-dose combination of stavudine, lamivudine, and nevirapine J Clin Virol ... nested RT-PCR and an in- house sequencing assay as described elsewhere [4] The interpretations of genotyping data and subtyping were performed using Smartgene™ HIV software packages (Integrated Database ... [http://www.biomedcentral.com/content/supplementary/17426405-6-12-S2.xls] 16 WHO: WHO case definitions of HIV for surveillance and revised clinical staging and immunological classification of HIV related disease in adults and children Geneva: World Health...
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Báo cáo y học: "Detection, characterization and regulation of antisense transcripts in HIV-1" ppt
Ngày tải lên :
13/08/2014, 05:22
... detection of HIV- 1 antisense transcript in infected cells lines and transfected 293T cells Specific detection of HIV- 1 antisense transcript in infected cells lines and transfected 293T cells Synthesis ... transcript in HIV- 1, which included the ASP ORF sequence The use of HIV- 1 proviral DNA clones and of infected cell lines suggested that a wide range of HIV- 1 strains are capable of producing this ... Figure Expression of HIV- 1 antisense transcript during viral infection Expression of HIV- 1 antisense transcript during viral infection (A) 293T cells were infected with 35 ng of NL4.3LucR-E- or...
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Báo cáo khoa học: "The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells" ppsx
Ngày tải lên :
07/08/2014, 23:22
... Banchereau J, Steinman RM Dendritic cells and the control of immunity Nature 1998, 392, 245-252 Blanco P, Palucka AK, Pascual V, Banchereau J Dendritic cells and cytokines in human inflammatory and autoimmune ... survival and maturation of DCs [11,17,20] To investigate further the mechanisms of taxol-induced changes, the viablity of TPCK, an inhibitor of the serine protease that inhibit NF-κB signaling pathway ... independent experiments are presented (A) The optical density of each band was divided by that of the β-actin band, and the ratio at h was set at 100% (B) Fig NF-κB involvement in the taxol-induced...
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Báo cáo y học: "Differential induction of inflammatory cytokines by dendritic cells treated with novel TLR-agonist and cytokine based cocktails: targeting dendritic cells in autoimmunity" potx
Ngày tải lên :
11/08/2014, 06:22
... CCL18, CCL22 and APRIL, Page of 12 involved in both Th1 and Th2 type responses [8,9] The unique and very complex signalling network in DCs involving PRRs and secretion of early triggers of inflammation, ... chemoattraction of these cells from the circulation into the site of inflammation The treatment in our in vitro model with cocktails containing Th1 inducing or inflammatory cytokines and TLR agonists ... measuring drug induced changes in expression of maturation markers and secreted cytokines and chemokines, inflammatory lipids, intracellular signalling Page of 12 molecules and ability to induce...
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Báo cáo y học: "Dendritic cells: In the forefront of immunopathogenesis and vaccine development – A review." doc
Ngày tải lên :
11/08/2014, 10:23
... dendritic cells with interleukin and CD40-ligand J Exp Med 1996, 185:1101-1111 Grouard G, Durand I, Filgueira L, Banchereau J, Liu Y-J: Dendritic cells capable of stimulating T cells in germinal ... great insights into the origins of DC subsets [6,7] and their modulation by distinct cytokines of neighboring cells [8,9] Progenitors of DCs in bone marrow migrate via the blood stream and home ... antigen-specific B cells [29] T- and B-cell interaction results in the clonal expansion of B cells, which takes place in the plasma foci of the T cell rich area [30] and in the germinal centers [31] T- and...
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Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus" pot
Ngày tải lên :
12/08/2014, 12:20
... marrow cells; FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte macrophage-colony stimulating factor; IL-4, interleukin ... marrow-derived dendritic cells; CD3- BMC, CD3- bone marrow cells; FLDC, dendritic cells induced by FL; FL, FMS-like tyrosine kinase ligand; GMCSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin ... levels of CD123 FLDCs, dendritic cells induced by FL; mDC, myeloid dendritic cells induced by GMCSF + IL-4; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte macrophage-colony-stimulating...
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Photoimmunotherapy of melanoma via combination of hypericin photodynamic therapy and in vivo stimulation of dendritic cells by PNGVL3 HFLEX plasmid DNA
Ngày tải lên :
28/11/2015, 13:45
... mobilization of DCs using Flt3 ligand, local injection of chemokines such as macrophage inflammatory protein (MIP)-3β, use of DNA vaccines containing bacterial CpG motifs and the use of topical compounds ... be discussed in detail later Intra-tumoral injection of DCs offers the theoretical advantage of in vivo loading and activation of DCs which should be superior to in vitro loading of DCs with tumor ... tumors and the number of memory T -cells in tumor draining lymph nodes as compared to PDT alone In another study, schizophyllan (SPG) used in combination with Photofrin-mediated PDT of mice harboring...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
Ngày tải lên :
14/02/2014, 18:20
... shown that interactions between KCs and endotoxin comprise the initiating event leading to hepatotoxicity in liver injury, including endotoxemia and ischemia ⁄ reperfusion injury [14] In our studies, ... line of defense against pathogens [7] TLR2 and TLR4 expression in multi-organs, including the liver, lung, heart and spleen, was significantly upregulated in experimental models of sepsis and in ... activity of CYP2E1 were downregulated in a rat hepatoma cell line after the administration of proinflammatory cytokines, leading to a loss of catalytic activity This downregulation was at the level of...
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Báo cáo khoa học: A peptide derived from cyclin-dependent kinase activator (p35) specifically inhibits Cdk5 activity and phosphorylation of tau protein in transfected cells pdf
Ngày tải lên :
23/03/2014, 21:21
... helices in p25 as determined by Tarricone et al [21] The sequences comprising p25, p16 and CIP are indicated by the labelled arrows (B) A combination of N-terminal and C-terminal truncations of p35 ... nonactivating fragment (154–279, CIP) that inhibits Cdk5 activity and binds with high affinity (C) Inhibition of Cdk5 activity by CIP Cdk5 kinase activity was determined by preincubating various amount of ... penicillin and 100 lgÆmL)1 streptomycin at 37 °C in a humidified atmosphere of 5% CO2 The cells were transiently transfected using LipofectAMINE (Life Technologies) according to the manufacturer’s instructions...
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báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot
Ngày tải lên :
18/06/2014, 15:20
... formation and maintenance of the histo-architecture Plasmonogen activator inhibitor-2 (PAI-2) Involved in the regulation and inhibition of binding between urokinase-type plasminogen activator and its ... protein for phosphoserin motifs Regulated the interactions and subcellular localization of signalling molecules γ-Catenin Forms a complex with α- and β-Catenin to Cadherins, that are involved in ... fixed in 50% ethanol, and 10% acetic acid for 12 hours Silver staining Proteins were visualized using the silver staining method employing a modification of the method of Heukeshoven according...
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báo cáo hóa học: " Nogo receptor is involved in the adhesion of dendritic cells to myelin" ppt
Ngày tải lên :
19/06/2014, 22:20
... Journal of Neuroinflammation 2011, 8:113 http://www.jneuroinflammation.com/content/8/1/113 repeat and Ig domain-containing, Nogo receptor-interacting protein) [12] However, due to the findings of ... Protein concentration of all lysates was determined with the bicinchoninic acid protein assay (BCA, Sigma-Aldrich) 22 μg of human brain, and 70 μg each of immDC and matDC protein were denatured and ... interaction of DCs with myelin provides insight into how DCs act when in the presence of CNS myelin, such as during neurodegeneration and/ or neuroinflammation The down-regulation of myelin-associated inhibitory...
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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc
Ngày tải lên :
20/06/2014, 01:20
... antiviral cytokine response of infected cells to a large extent Interestingly, however, induction of the chemokines IP-10 and IL-8 escaped suppression by SARS-CoV in Caco-2 cells, but not in HEK 293s ... Figure Interleukin production Interleukin production RNA samples of Caco-2 cells (A) and HEK 293 cells (B) described in Fig were investigated by RTPCR for the presence of IL-6 and IL-8 mRNAs by infected ... organs, including the lungs [8,14] Our data may explain this rapid and efficient dissemination of SARS-CoV By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, ...
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Báo cáo khoa học: "Comparative study of endocrine cells in the principal pancreatic islets of two teleosts, Silurus asotus (Siluridae) and Siniperca scherzeri (Centropomidae)" potx
Ngày tải lên :
07/08/2014, 14:23
... hypothalamus of sheep, and exists in straight and cyclic forms (Brazeau et al., 1973) This substance inhibits the secretion of the gastrin, cholecystokinin, secretin, glucagon, insulin, motilin and gastric ... immunohistochemical study of the endocrine cells within the pancreas, intestine, and stomach of the gar Gen Comp Endocrinol 1997, 106, 1-16 Guyot, E., Diaz, J P., Romestand, B and Connes, R Insulin during the ... glucagon, somatostatin and PP, in the pancreas of teleosts, localization of endocrine cells in the principal pancreas of Silurus asotus and Siniperca scherzeri have not yet been reported In addition,...
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Báo cáo khoa học: " An immunohistochemical study of chromogranin A and Sp-1 immunoreactive cells in the gastrointestinal tract of ovariectomized rats" pdf
Ngày tải lên :
07/08/2014, 18:21
... htiw demrofrep saw noitcaer cificepsnon fo gnikcolB ]13[ dohtem )PAP( esadixorep itna-esadixorep eht htiw deniatsonummi dna detardyher ,deziniffaraped saw noitces evitatneserper hcaE gniniats lacimehcotsihonummI ... etanobracylop rep detacolla erew slaminA syad rof noitazitamilcca retfa desu erew )napaJ ,reviR selrahC ;tpiecer nopu dlo kw-6( tar elamef )DS( yelwaD-eugarpS ytnewT o slamina latnemirepxE sdohteM dna ... lanoitubirtsid eht ,hguohtlA ]8,3[ sllec enircodneoruen lla fo ”srekram“ nommoc yltnacifingis a ,stneitap aitreni cinoloc nI slamina fo sutats evitsegid ot detaler ylraelc erew sllec RI-GC fo segnahc eht...
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Báo cáo toán học: "Expression and function of micro RNAs in immune cells during normal or disease state" pptx
Ngày tải lên :
08/08/2014, 17:20
... -15b and let-7f were downregulated in effector cells compare to naïve cells and increased back in memory T cells [14] Effector T cells originate from naïve T cells following antigen exposure and ... role in innate and acquired immune response Elucidating the mechanisms by which miR-155 expression is controlled and finding its targets will bring more understanding of the mechanisms of tumor induction ... epithelial cells) and regulates TLR4 expression Infection of these cells with Cryptosporidium parvum (parasite that causes intestinal and biliary diseases) results in the reduced expression of let-7i in...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
Ngày tải lên :
09/08/2014, 01:21
... centrifugation Cells were resuspended in buffer containing 0.1% bovine serum albumin and 0.1% NaN3 at 106 cells/ ml Nonspecific antibody binding at the Fc receptor was blocked by treating 106 cells with ... constitute part of the expanded population of memory T cells bearing chemokine receptors in WG A lack of CD27 expression has been reported to distinguish effector cells from naive T cells within the CD8+CD45RA+ ... T cells cannot be based on the analysis of single surface antigens A combination of surface markers and the function of the T cell have to be considered in distinguishing naive from memory T cells...
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