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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
Ngày tải lên :
18/02/2014, 17:20
... by DNA sequencing. The
design of the mutants was intended to preserve the
amino acid character and to modify only the relative
volume of their R groups.
The expression of the FNR mutants as soluble ... phe-
nol as the tyrosine R group. This system has been used
previously to analyze the geometry of the tyrosine
stacked on the si-face of the flavin in FNRs [12]. The
single point energies of the flavin–tyrosine ... in the protein stability of our mutants. There-
fore, these conclusions strengthen our hypothesis that
the catalytic efficiencies of the recombinant enzymes
used in our study were related to the...
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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
Ngày tải lên :
19/02/2014, 12:20
... found with other
dissociated capsid proteins, the lack of elution of the
proteins can be explained by non-specific binding of the
denatured coat proteins to the gel [34,35]. The elution of
some D11N ... Figure 5C shows that the spectra of the
sample subjected to compression and decompression are
similar to the non-treated sample, even in the case of the
M88V mutant. The elution of the samples after ... decrease the stability of the capsid. Our findings
illuminate the role of packing in the icosahedral lattice of
the virus capsid.
Experimental procedures
Chemicals
All reagents were of analytical...
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Tài liệu Fertility, Family Planning, and Reproductive Health of U.S. Women: Data From the 2002 National Survey of Family Growth doc
Ngày tải lên :
13/02/2014, 10:20
... disagreement about the
intendedness (at time of conception) of
recent births, with the father’s attitudes
based on the mother’s reports of his
attitude. A forthcoming report will
describe fathers’ attitudes ... attitudes toward the
intendedness of pregnancies based on
their own reports (13).
+ Of all births in the 5 years before
interview, 55 percent were intended
by both the mother and the father
(as ... (tables
97,98)
The NSFG is one of the few
reliable sources of nationally
representative data on the use of
infertility services. Earlier reports have
documented the rise in use of these
services...
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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt
Ngày tải lên :
16/02/2014, 09:20
... initiation of apoptosis through
the mitochondrial pathway. The key event in the mito-
chondrial pathway is the release of proapoptotic fac-
tors from the mitochondrial intermembrane space into
the cytosol, ... release
of cytochrome c and second mitochondria-derived
activator of caspase (Smac ⁄ DIABLO) allows for the
formation of the apoptosome, a complex that enables
the activation of caspases within the ... The Authors Journal compilation ª 2010 FEBS 1325
The ubiquitin ligase Itch mediates the antiapoptotic
activity of epidermal growth factor by promoting the
ubiquitylation and degradation of the...
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Tài liệu Faultlines of Conflict in Central Asia and the South Caucasus - Implications for the U.S. Army docx
Ngày tải lên :
17/02/2014, 11:20
... not
understand the “rules of the game” the way the United States does.
That is one interpretation of both the crude Chinese nuclear threats
over the Taiwan crisis and Chinese views of the U.S. bombing of the
Chinese ... confronta-
tion between the Soviet Union and the United States was the central
reality in world politics. With the end of the Cold War and the disso-
lution of the Soviet Union, the world continues ... result of agreements
with the United States and unilateral actions in the aftermath of the
Cold War, and partly because of the state of the Russian economy.
Even if Russia had not ratified the Strategic...
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Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
Ngày tải lên :
18/02/2014, 18:20
... contact with the carbonyl oxygen of Gly255.
These movements away from the oxidized cysteine
increase the space inside the P-loop to accommodate
the large sulfonic acid group. The movements in the
P-loop ... serve to provide room for the larger oxidized
cysteine. The key feature that dictates whether the
P-loop moves upon oxidation of the catalytic cysteine is
the ability of the conserved active site ... with O
d
2 of the cysteine sulfonic acid. In addi-
tion to these contacts, there is a 1.82 A
˚
contact
between Gly255 C
a
and O
d
2 of the oxidized cysteine.
To further understand the structural...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
Ngày tải lên :
19/02/2014, 02:20
... glycation, rather than
purely the loss of the parent amino acid, which is the
key factor in terms of receptor recognition [29]. There
are no data available on the extent of Lys (and other
amino ... receptor-blocking
experiments. The rates of uptake of both the lipid and
protein components are not matched by the rate of cel-
lular metabolism of these species, resulting in the accu-
mulation of both unmodified ... and
control LDL, for 96 h. The use of LDL-free medium
during the chase period allows the turnover of preaccu-
mulated protein to be studied in the absence of further
cellular uptake. In these studies, cell...
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Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
Ngày tải lên :
19/02/2014, 17:20
... by the replacement of Fe by Zn in the
rubredoxin given the identical structures of the two
protein forms [38,39]. Therefore, at the concentrations
used in the NMR experiments over 90% of the
cytochrome ... of the haem methyl groups in the fully reduced stage 0 are not plotted because they are unaffected by
the pH. The solid lines represent the best fit of the shifts for DvHc
3
:Pi to the model of ... is bound to the rubredoxin and the effect
observed in the signals of the haem methyls of
DvHc
3
:ZnRb complex vs. the results in the isolated
cytochrome is close to the full effect of complex...
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Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
Ngày tải lên :
20/02/2014, 02:21
... productive binding of steroid substrates:
in the first, a (small) sub population of enzyme binds
directly the steroid in the reactive conformation, with
the remainder of the enzyme binding the unreactive
conformation. ... grossly perturb the
overall framework of the enzyme or the active site. The active sites
of the enzymes are shown in stick format with the sigmaA weigh-
ted 2F
0
-F
c
density for the Y186F mutant ... bond in
close proximity of the N5 atom. A putative flipping
motion of the progesterone molecule along an axis
parallel to the FMN plane aligns the C1-C2 with the
plane of the isoalloxazine ring...
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Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx
Ngày tải lên :
20/02/2014, 23:20
... of the structure of AII in solution with the X-ray
structure of AII complexed to the Fab131 mAb. In Fig. 7A, a
sequence alignment is represented of the backbone of the
conformational ensemble of ... alignment of the fragment 4–7 of the backbone of
the 13 ensemble solution structures of AII (brown colour) superimposed
on the X-ray structure (dark blue colour) and (B) structure alignment of
the ... of
the fragment 4–7 of the 13 ensemble structures of AII to the fragment 3–6
of the X-ray structure of AII.
Fig. 6. Structure of a representative folded conformer of AII showing the van der Waals...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf
Ngày tải lên :
21/02/2014, 03:20
... 3B). The pK
2
increased as the size of the residue at position 169 increased.
The effect of the size of the residue at position 169 may be
related to the solvent accessibility of the side-chain of
His210 ... was supported by the
independency of the ionic strength of the pH-activity curve
(Fig. 5).
In the X-ray crystal structure, the Trp169 side-chain is
located on the outside of the His210 side-chain ... Accordingly, the pK
2
on the
acidic rim of the Trp169 mutants decreased with decreasing
size of the residue at 169 (Fig. 3).
Although the structural arrangement of this stacking
implies that the interaction...
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Báo cáo khoa học: The A domain of fibronectin-binding protein B of Staphylococcus aureus contains a novel fibronectin binding site pdf
Ngày tải lên :
06/03/2014, 00:20
... promote
the bacterial invasion of endothelial cells.
To explore the biological significance of the interac-
tion between the A domain of FnBPB and Fn, the
ability of the A domain, in isolation from ... antigenically distinct [10,11].
The A domain of ClfA and FnBPA bind Fg at the
C-terminus of the c-chain [7]. The interaction between
the A domain of ClfA and the c-chain of Fg has been
studied in detail. ... [15]. They are located in the
equivalent positions to the aforementioned residues
P336 and Y338 of ClfA. One of the objectives of this
study was to determine the mechanism of Fg binding
by the...
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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
Ngày tải lên :
06/03/2014, 09:22
... in the T
and TG structures are aligned.
b
Backbone atoms of Bacillus gluta-
minase are superimposed on those of the liganded enzyme.
c
The
value of Tyr27 is excluded from the calculation of the ... report the crystal structure of the intact glu-
taminase under four different conditions: in the absence
of the additives (referred to as N); in the presence of
Tris (referred to as T); in the presence ... state
(disordered in the ligand-free state in YbgJ). In the
absence of the ligand, the lid part is separated from
the active site, which leads to a more open active site.
Upon the binding of ligand in the acyl-enzyme...
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“Marketing is the whole business, taken from the customer’s point of view.” - Peter Drucker docx
Ngày tải lên :
06/03/2014, 21:20
... Shoppers
Electronically or the Old Fashioned Way
Market newsletters are a great method of reminding your
shoppers of why they love the market. The trend in this type
of communication is the e-newsletter ... Marketing the Market
Katherine Kelly and Joan Vibert, vendors at Kansas City’s
Brookside Farmers’ Market, offer the following possibilities
for vendors to contribute to the success of the market ... vendors may not
achieve their potential.
Through marketing the market, many of the preced-
ing relationships can been strengthened. When vendors
make the effort to market the market as a whole...
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Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx
Ngày tải lên :
07/03/2014, 03:20
... counteracts
the effect of the mutations, stabilizing the dimeric
form. The stabilizing effect is even more pronounced if
l-serine binds to the cofactor at the active site, as is
evident in the case of the ... region in this group of enzymes, interacting with
the phosphate moiety of PLP. Therefore, the capability
of the third cluster of CHCs to confer stability to the
polypeptide loops of the active site ... structure.
Discussion
The decrease of hydrophobic contact area determined
by the mutations in the third cluster of CHCs caused a
shift of the equilibrium between dimeric and monomeric
forms of eSHMT in favor of the...
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