... titration. Inthe fits, the protein concentrationwas left free, to serve as an indicator of stoichiometry. In the case of 1IR, the fit resulted in a binding stoichi-ometry of 2 : 1, i.e. dimer binding. ... S1),indicating that it is static in nature. We do not havean unequivocal interpretation ofthe origin ofthe staticquenching. However, judging from the binding stoichi-ometry together with the ... available, indicating secondary struc-tural changes inthe linker connecting the dimerizationand DNA-binding domains, and rearrangement of the relative orientation ofthe two domains [7,9]. The con-formational...
... recorded the acidity inthe rain water in April, May yearly. No using of rain water in early rainy season is recommended as way to treat the air-dust and other pollutants deposited in the house-roofs ... inthe coastal lands ofthe delta. Saline encroachment effects almost half ofthe MD. The salinity is caused by sea water intrusion in open mouths and estuaries. The phenomenon of salinity ... bars inthe sea in front ofthe estuaries. Otherwise, in early rainy season, nearly 1,6 million hectares ofthe delta are infected by the acidity water due to the washing out aluminium and iron...
... vulnerability as they manifest inthe past and present of Alaska Native foodways. The first paper, ‘Outpost Gardening in Interior Alaska’ examines the historical dimensions of cropping by Athabascan ... high-frequency of forest fires collects within the basin ofthe Alaskan interior, and during 8 Whether real or perceived, the coldness of river water is mentioned often in BIA reports from schoolteachers ... political economies of cheap food increases, outpost agriculture is finding a renewed niche in emerging indigenous movements against the vulnerabilities embedded within participation inthe cheap food...
... 1995). In the past few years, there has beenan increase inthe reported inci-dence of torture — cruelty by the husband and the husband’s rela-tives. The reported number of incidents of torture increased ... compo-nents ofthe spread of HIV. Sur-veys in some areas show 5 to 10percent of truck drivers in the country are HIV infected (AIDSAnalysis, 1996).Despite the alarming growth of the epidemic, ... to turn to theirparents if they leave their husbandsbecause their parents either will not(because ofthe social stigma) orcannot (because of economics)take them in. Generally, the policehave...
... graph obtained from G by contracting the edges in E1and delet-ing the edges in E2. Let H be the graph obtained from Zdby contracting the edges in K1and deleting the edges in K2. By ... U(W; x, y) the intersection of U(W) with the event that the path connecting x, y inthe USFis edge-disjoint from the oriented USF paths connecting the vertices in V :=W \{x, y} to in nity. By ... ΥR, the paths in F to ΥRfrom the vertices in the complement of ΥR∪ B(x, R) are conditionally independent of γR. Therefore the conditional distribution of ζRgiven ΥRis just the harmonic...
... insulinreceptor-interacting region (PIR) [6] and inhibits the tyrosine kinase activity ofthe IR [7]. On the basis of the crystal structure ofthe tyrosine kinase domain of the IR in complex with the PIR ⁄ BPS domain ... residues of the IR kinase loop, which help position the PIR–BPS andincrease binding affinity [8]. Although the interaction of Grb14 with the IR is probably the major determi-nant ofthe insulin-induced ... amodel for the Grb14–IR interaction. The PIR–BPSbinds as a pseudosubstrate inhibitor inthe substrate-binding groove ofthe kinase, whereas the SH2 domaininteracts with phosphorylated tyrosine residues...
... cells were incubated for 24 heither inthe absence or in prese nce of FSH, dbcAMP,cholera toxin or bFGF, either in combination with FSH ordbcAMP or cholera toxin and bFGF.Extraction of total ... [5154]. Inhibitory transcription factorsthat bind to AP-1 or FIN-1 o r that inactive b inding ortransactivation capacity of, for example, FIN-1 and USF-1,could explain activation or inhibition of ... partners of bFGF (Eur. J. Biochem. 269) 509 The bFGF binding requires speciđc motifs on highlysulfated HS chains. Sodium chlorate, in inducing structuralalteration of HS chains, prevents the bFGF...
... results in blocking ofthe binding ofthe SH2 domain to the substrate and thereby preventing interaction of the substrate with the kinase domain. For active n-Src in which the C-tail tyrosine was ... demonstrated that intra-domain interactions betweenSrc family kinases (SFKs), stabilized by binding ofthe phosphorylatedC-terminus to the SH2 domain and ⁄ or binding ofthe SH2 kinase linker to the SH3 ... studies of inactive andactive Src, the SH2 and SH3 domains are believed toform a ‘regulatory apparatus’. Binding ofthe phos-phorylated C-terminus to the SH2 domain and ⁄ orbinding ofthe SH2-kinase...
... ∞ THE FINANCIAL CRISISINQUIRY REPORTFINAL REPORT OFTHE NATIONAL COMMISSION ON THE CAUSES OFTHE FINANCIAL AND ECONOMIC CRISIS INTHE UNITED STATESOFFICIAL GOVERNMENT EDITION THE FINANCIAL ... Oce ofthe Comptroller ofthe Cur-rency and the Oce of Thrift Supervision, caught up in turf wars, preempted stateregulators from reining in abuses. CONCLUSIONS OFTHE FINANCIAL CRISIS INQUIRY ... this inquiry. In the course of its research and investigation, the Commission reviewed millions of pages of documents, interviewed more than witnesses, and held days of public hearings in...
... his mind, pro-gress lay inthe merging ofthe individual inthe family, ofthe family in the commonwealth, ofthe commonwealth inthe continent, and of the continent inthe world. Finally, the ... after the renewed German attacks inthe twenties; and the Divinity of our Lord, some think, had gone all but in name by the beginning of the century. The Kenotic theory had provided for that. Then ... hydraulic power into the centre ofthe dining-table, had be-come universal inthe houses ofthe well-to-do. The floor consisted en-tirely ofthe asbestos cork preparation invented in America, noiseless,clean,...
... However, the amino acidsforming the catalytically active center ofthe A-chains of the MLp toxins are fully conserved and retained in corresponding positions, as inthe A-chain of ricin [27] ... Gln256 ofthe ricin B-chain in the second sugar binding site [28]. The exceptions are the replacement of Trp38 fi Ser inthe ML2p B-chain andTyr252 fi Phe inthe ML3p B-chain (Fig. 2). The highly ... ricin [40]. The introduced mutation of Trp37 fi Ser noticeably decreased the efficiency of its binding to Gal. Inthe second binding site of the ML3p and ML3.1p B-subunits, the replacement of the...
... of all classes, the hatred ofthe Commons, the defection of the Lords, the alarm ofthe Church, and the wavering loyalty ofthe army.William, however, was already informed of these facts, and ... dispute being the succession to the crown of Spain, England's feelings inthe matterbeing further imbittered by the recognition by Louis XIV ofthe Pretender as King of England. Therefore,although ... conditions of this treaty became known theyinspired natural indignation inthe minds ofthe people ofthe country which had thus been arbitrarily allotted,and the dying Charles of Spain was infuriated...
... interest in improving working and living conditions inthe shipping industry. The Office’s programme on decent work in the maritime industry played a major role in improving the promotion of ... relating to the health of seafarers, often through the Joint ILO/WHO Committee on the Health of Seafarers. Since the Committee’s last meeting in 1993, 3 the two Organizations held, in 1997, the ... view to reducing the prevalence of gender stereotypes within the industry. Training 145. For the purpose of guaranteeing equality of opportunity and treatment in access to training, maritime...
... is the shift ofthe pH optimumfrom near neutral inthe wild-type to pH 9.2 inthe R389Amutant, which is in accord with an increase inthe pK of Y229 if the stabilizing R389 residue is lacking. ... coordination to the otherligands ofthe binding site (center I), but the bindingbecomes much weaker as emphasized by the dramaticincrease ofthe Na+ion concentration required to saturate the enzyme. ... carboxybiotin, or slow Na+move-ment through the channel to its binding site.Following the decarboxylation of carboxybiotin in the reaction cycle (Fig. 4B,C), the biotin prosthetic group leavesthe...
... higher during the tests performed inthe afternoon (P Ͻ 0.001). The intensity ofthe 6-MWT corresponded to 79.6 Ϯ 4.5% of the V˙O2max, 85.8 Ϯ 2.5% ofthe HRmax, and 78.0 Ϯ 6.3% ofthe HRreserve. ... concerning the distance,walking speed, V˙O2, and % ofthe V˙O2max. Otherwise, HRand % ofthe HRmaxare lower during the tests performed in the morning. In this population, the 6-MWT ... effect ofthe familiarization on the 6-MWT performance and the relative intensity of this test. The aims of this study were therefore to bring precision to the 6-MWT reliability and intensity in...