long term culture of chorionic villus samples

... of Cre recombinase, but expresses A new method for mouse endothelial cell culture CAG loxP βgeo pA tsA58T loxP CAG pA tsA58T T26 Tg T26/Tie2-Cre Tg Tie2-Cre Tg Enzymatic digestion of organs Culture ... the control of the CAG promoter, followed by several cloning sites including a SwaI site, polyadenylation signal sequence of the Pgk gene, and a portion of the multiple cloning site of pMCS5 XhoI–EcoRI-digested ... and cultured for 24 h at 33 °C Uptake of DiI-labeled acetylated LDL into tsA58T-expressing endothelial cells DiI-labeled acetylated LDL was added to the culture medium at a final concentration of...

Ngày tải lên: 18/02/2014, 17:20

... large number of samples that were tested, HCV isolation and long term replication were carried out in several phases: short term cultures (positive for HCV up to 10 weeks), medium term cultures (positive ... detected in cell cultures of a variety of cell types, the presence of positive-strand HCV-RNA persists for periods ranging from a few days to several months, albeit with no evidence of infectious ... cell-free transmission of HCV We did not compare the levels of virus produced by these two methods An example of a long term positive cell culture is isolate #081 This isolate was obtained from similarly...

Ngày tải lên: 19/06/2014, 08:20

... décontamination / multiplication long terme/Quercus robur L/maté/ matériel juvénile INTRODUCTION In vitro culture establishment from shoot- tip explants potentially offers kinds of advantages in cloning ... case of adult clones (Juncker and Favre, 1989; Slak and Favre, 1990) or cially We therefore tested methods of shootculture to improve the initial decontamination and the potential for long- term ... became necrotic within weeks of culture, whatever the type of source-plant On BM Cyt, the reactivity was better Shoot-tip explants enlarged within the first weeks of culture During the third week,...

Ngày tải lên: 08/08/2014, 19:21

... (saliva) consist mainly of secretory IgA (sIgA) [19] This study is the first, to our knowledge, to describe the long term persistence of IgE anti-Influenza virus antibodies in serum of IgE positive and ... B/Brisbane/60 /2008 Each 0.2 mL dose of Flumist intranasal spray contains 10 FFU (fluorescent focus units) of live attenuated influenza virus reassortants of each of the three strains for the 2009-2010 ... studies are the first to describe the long- term persistence of IgE anti-Influenza virus antibodies in vaccinated children and adults, with normal and elevated levels of serum IgE, approaching two years...

Ngày tải lên: 25/10/2012, 11:10

... participants with the images of car exteriors spanning a long period of time The specific usage of historic views of design-oriented objects such as cars should provide a sensitive test of how the design ... adaptation effects any more They are no longer in everyday use They are part of museums now, not of everyday phenomena Consequently, they are stable in terms of preferences for them and major design ... discussion In the present studies, we investigated long- term dynamics of design properties To be able to assess a long time period, we used b Residuals of the production numbers (all brands) production...

Ngày tải lên: 19/02/2014, 17:20

... whether future long- term gynecological cancer survivors will differ from present survivors in terms of QoL and symptoms More research is needed on the QoL of long- term survivors of gynecological ... Quality of life in long- term survivors of cervical cancer Maturitas 32(2), 95–102 (1999) Expert Rev Obstet Gynecol 5(1), (2010) Factors related to quality of life in long- term survivors of gynecological ... A critical review of patient-rated quality of life studies of long- term survivors of cervical cancer Gynecol Oncol 102(3), 563–572 (2006) 13 Anderson B, Lutgendorf S Quality of life in gynaecologic...

Ngày tải lên: 05/03/2014, 15:20

... problem of the public perception of monetary policy, since the de…nition of the endpoint is free, but to improve long- term forecasts with shifting perceptions of long term endpoints (or goals) of ... relationship between this perception and long- term bond rates For German data, the use of a two-factor model of the term structure results in the best forecast of long- term interest rates for the period ... relationship, the presence of shifts in the long- term perception of the short -term interest rate Finally, some conclusions are drawn in section Monetary policy, in‡ ation target and long term interest rates...

Ngày tải lên: 22/03/2014, 23:20

... family size [22] A model of how balance is maintained through proper positioning of the CoM and momentum of the CoM over the base of support has been established as a measure of dynamic balance control ... the remediation of any part of attention helps in performance during dual-task walking or that remediation of spatial orientation of attention is correlated with the recovery of other attention ... their particular activity at the time of injury at either the college or professional level, or have since graduated and are no longer active Recently, tests of balance control during an attention...

Ngày tải lên: 19/06/2014, 08:20

... patient’s symptom of radial head subluxation and lost concave deformity of radial head without symptom of forearm rotation and the sign of plain radiographs showed arthrosis of the elbow joint ... of the elbow J Bone Joint Surg 1991, 73A:440-446 Cohen MS: Lateral collateral ligament instability of the elbow Hand Clin 2008, 24:69-77 Josefsson PO, Johnell O, Gentz CF: Long- term sequela of ... doi:10.1186/1749-799X-5-5 Cite this article as: Cheng: Long- term sequel of posterolateral rotatory instability of the elbow: a case report Journal of Orthopaedic Surgery and Research 2010 5:5 Submit...

Ngày tải lên: 20/06/2014, 04:20

... comparison Microsoft Excel (Microsoft, Redmond, Washington) and MedCalc statistical software (MedCalc Software bvba, Mariakerke, Belgium) were used for the analysis Results Of the original 30 ... the longer term survivorship and clinical outcomes of navigated and non-navigated UKAs A larger proportion of well aligned knees had good or excellent clinical outcomes and a higher proportion of ... the small number of patients in our study All but two knees had a range of flexion beyond 100 degrees; we did not find a correlation between range of movement and alignment of the leg There was...

Ngày tải lên: 20/06/2014, 04:20

... good range of motion with a mean and SD external rotation of 81.39 ± 8.12 degrees A total of shoulders in patients had a recurrence of shoulder instability Of the 6, of the recurrence of dislocation ... effectiveness of the arthroscopic Bankart repair The shoulder rating scale of University of California Los Angeles (UCLA) [6] and the simple shoulder test (SST) [7] The SST consisted of a series of 12 ... Female Number of shoulders* 79 (5) Mean number of dislocations before surgery (range) 11.17 (1-100) Mean duration of operative time (range) 64.56 (35-145) Mean pre-operative range of external rotation...

Ngày tải lên: 20/06/2014, 04:20

... comparison Microsoft Excel (Microsoft, Redmond, Washington) and MedCalc statistical software (MedCalc Software bvba, Mariakerke, Belgium) were used for the analysis Results Of the original 30 ... the longer term survivorship and clinical outcomes of navigated and non-navigated UKAs A larger proportion of well aligned knees had good or excellent clinical outcomes and a higher proportion of ... the small number of patients in our study All but two knees had a range of flexion beyond 100 degrees; we did not find a correlation between range of movement and alignment of the leg There was...

Ngày tải lên: 20/06/2014, 07:20

... good range of motion with a mean and SD external rotation of 81.39 ± 8.12 degrees A total of shoulders in patients had a recurrence of shoulder instability Of the 6, of the recurrence of dislocation ... effectiveness of the arthroscopic Bankart repair The shoulder rating scale of University of California Los Angeles (UCLA) [6] and the simple shoulder test (SST) [7] The SST consisted of a series of 12 ... Female Number of shoulders* 79 (5) Mean number of dislocations before surgery (range) 11.17 (1-100) Mean duration of operative time (range) 64.56 (35-145) Mean pre-operative range of external rotation...

Ngày tải lên: 20/06/2014, 07:20

... known about the longer term outcomes of these large-scale rollouts A recent review of ART programs in sub-Saharan Africa suggested that, due to loss to follow up and death, only 60% of those initiating ... high mobility of the population Generalizability of results may be limited because of the use of data from a single clinic located at a tertiary care hospital, the dominant use of EFZ-containing ... use of time-to-event analysis instead of cross sectional assessment, thus overcoming the limitation of overrepresentation of patients with short follow-up Our analysis also has a number of limitations,...

Ngày tải lên: 20/06/2014, 08:20

... cases of recursive VQ [2, 10], depending on the configuration In the following, the term long- term refers to considering long sections of speech, including several to many short -term frames of ... analysis of the results with the voiced sections Figure displays the results of the LT coding technique in terms of ASD as a function of the bit rate Each one of the curves on the left of the figure ... organized as follows The proposed long- term model is described in Section The complete long- term coding of LSF vectors is presented in Section 3, including the description of the fitting algorithm and...

Ngày tải lên: 21/06/2014, 16:20

... equivalent) of tephra fall deposits, making it one of the largest explosive eruptions of the 20th century (Kratzmann et al 2008) The elongated shape of the deposit was the result of strong northwest ... resulted in evacuations out of the area in the short and long term The following types of responses were identified from interviews: • Short -term (

Ngày tải lên: 21/06/2014, 17:20

... equivalent) of tephra fall deposits, making it one of the largest explosive eruptions of the 20th century (Kratzmann et al 2008) The elongated shape of the deposit was the result of strong northwest ... resulted in evacuations out of the area in the short and long term The following types of responses were identified from interviews: • Short -term (

Ngày tải lên: 21/06/2014, 19:20

... Cellular layout Sectors Number of cell sites Antenna gain of the base station Width of beam of the antenna at −3 dB Front/Back ratio of the antenna Antenna pattern radiation of the base station Propagation ... and several traces of fading values are provided for each pair of antenna A uniform distribution of mobile users is generated at the beginning of each simulation Typical number of users chosen for ... evaluated these two forms of multiresolution considering the WCDMA technology in [14–16] In OFDMA-based networks, the transmission of different fractions of the total set of subcarriers (chunks)...

Ngày tải lên: 21/06/2014, 23:20

... attention needs to be paid to the long term follow-up of both children and adults who receive this form of therapy Journal of Biology 2006,5:21 21.4 Journal of Biology 2006, Volume 5, Article ... no longer valid Further evidence of the development of methotrexateinduced leukoencephalopathy in the absence of cranial radiation was shown in a German study of infants with medulloblastoma treated ... perspective, concerns about the long- term effects of CNS radiation were first raised in the early 1980s regarding children treated for brain tumors Our group had reported that, of 10 children with posterior...

Ngày tải lên: 06/08/2014, 18:21