localization of ebers and their interaction with cellular proteins

... stoichiometry of the complex between RI and RATEs, 100 or 200 pmol of RI was incubated either with 100 pmol of SGRSGRSG-RATE or with 100 pmol of RNase A at 25 °C for 15 in 15 lL of 0.1 m sodium ... catalytically active and that the activity decrease [16] is a result of the tandemization Accessibility of the linker of SGRSGRSG-RATE in the presence of RI The accessibility of the linker sequence ... the other hand, the decreased activity of the RATEs as compared with RNase A ([16] and Table 1) indicate a negative influence of the tandemization on the catalytic efficiency The recovery of activity...

Ngày tải lên: 22/03/2014, 16:21

... because of their unique properties that differ from their linear analogues However, examples of these molecules are rare, mainly attributed to the difficulty in their preparation and lack of the ... aromatic oligoamides with biomacromolecules, such as oligonucleotides and proteins The aim of this thesis was to investigate the structural and physical properties of a 13 new class of backbone-rigidified ... specificity and affinity Figure 1.6 Solid state structure of L-tartaric Acid-binding M-1 helix (a) CPK and (b) stick representations of the M-1 helix (c) Top view of the central part of the M-1...

Ngày tải lên: 10/09/2015, 15:49

... angle of 63º between axis ac and plane of the molecules, all the other three dimers (2a, 2c and 2d) are stacked along axis a with angles of 90º, 53º and 70º, respectively, between axis a and plane ... study and elucidation of the higher ordered structures of these folding molecules with interesting properties and applications Many aspects of the synthetic chemistry associated with this class of ... from 3a and generated by breaking H-bond of Oa···Hb (1A & 1B), Hb···Oc (2A & 2B), Oc···Hd (3A & 3B) and Hd···Oe (4A & 4B), and by breaking H-bonds of both Oa···Hb and Hb···Oc (5A & 5B), and both...

Ngày tải lên: 10/09/2015, 15:49

... previously.3c Figure 3.1 Side and top views of oligoamides and 6: (a) crystal structure of pentamer 5a, (b) ab initio calculated structure of 5a and (c) crystal structure of hexamer 6a Interior methoxy ... by slow evaporation of 5a and 6a in mixed solvents containing hexane and chloroform 91 (1:1 v/v) for 5a and hexane and dichloromethane (1:1 v/v) for 6a at room temperature Their crystal structures ... protons and their adjacent interior methoxy protons, (f) Chemical structure of 6b More NOESY, ROESY and TOXSY studies of 6b were demonstrated in Figure 3.8 and Figure 3.9 Figure 3.8c and 3.8d...

Ngày tải lên: 10/09/2015, 15:49

... indicator of the presence of strong H-bonding interactions, leading to the rigidification of the amide linkages and a crescent aromatic backbone Figure 4.2 1H NMR spectrum (500 MHz, 298 K, CDCl3) of ... structure of pentamer (a) top view with interior methoxy methyl groups omitted for clarity of view, (b) top view and (c) side view both with methoxy methyl groups in CPK representations 4.2.5 Design of ... ppm as a result of the H-bonding ability of DMSO 4.2.7 Crystal Structure of a Tetramer and Computational Molecular Modeling of Circular Pentamer To demonstrate the existence of intramolecular...

Ngày tải lên: 10/09/2015, 15:49

... analysis of inhibitory effect of 10a in 50 mM NaCl, (d) PAGE analysis of inhibitory effect of 11a in 50 mM KCl and (e) PAGE analysis of inhibitory effect of 11a in 50 mM NaCl (f) Comparison of inhibitory ... other hand, the quantity of PCR product of 11a in Na+ solution also decreased sharply compared to 10a, indicating the decrease of the ligands’ binding selectivity in company with the increase of ... 5.14 Influence of 10a and 11a on PCR product by using ODN-3 as the PCR template: (a) CD spectra of ODN-1 in the presence of 100 mM KCl and NaCl, (b) PAGE analysis of inhibitory effect of 10a in 50...

Ngày tải lên: 10/09/2015, 15:49

... presence of many proteins was tested As expected, a mixture solution of 10-fold excess of human γ-thrombin, BSA and Factor Xa did not interfere with the detection of trace levels (10 nM) of human ... both Au-T6 and thrombin Our earlier observation and discussion in the duplex approach shows that the interaction of TBA with its complementary DNA strand is stronger than that between TBA and thrombin ... spectra of gold nanoparticles before and after modification with DNA Two Thrombin aptamers TBA (15-mer) and TBA2 (29-mer) and their analogues were chemically coupled to AuNPs via the formation of...

Ngày tải lên: 10/09/2015, 15:49

... oligomers and TBA2: Au-TBA2 with (a) thrombin only, (b) with both thrombin and 1, (c) with both thrombin and 9a, (d) with both thrombin and 10b, (e) with both thrombin and 11a, (f) with thrombin and ... efficient and specific identification of G-quadruplex-binding ligands Figure 7.13 Aggregation states of Au-TBA in the presence of both lysozyme and pentamers: (a) 10a, (b) 11a , (c) 10b, (d) 9a, and ... Figure 7.4 UV-vis spectra of Au-TBA with both thrombin and 10a and with thrombin only 7.2.3 Sensitivity of Aptamer-Based AuNPs Screening Assay To investigate the sensitivity of aptamer-based AuNPs...

Ngày tải lên: 10/09/2015, 15:49

... unit mass of reactants due to chemical reaction, and γ ratio of the specific heat of the gas ρ1 , p1 , and u1 represent the density, pressure, and velocity of the unburnt gas, and ρ , p2 , and u2 ... size of 0.2mm 145 Fig 5.29 Structure with one transverse wave with the mesh size of 0.05mm 146 Fig 5.30 Structures with One Transverse Wave with the mesh size of 0.025mm 147 Fig 5.31 Formation of ... study 89 Fig 4.1 Profile of pressure at time = 320 µ s 96 Fig 4.2 Profile of flow velocity at time = 320 µ s 96 Fig 4.3 Profile of temperature at time = 320 µ s 97 Fig 4.4 Profile of density at time...

Ngày tải lên: 11/09/2015, 16:07

... Hanbin, Dr Fernando and Dr Anuja, for their friendship and valuable discussion during the study Special thanks go to Mr Cheng Yonggang for helping me to solve the problems of CAD and other softwares ... the whole study without the great love and care from you iii Table of Contents Acknowledgements ii Table of Contents iv Summary viii List of Tables x List of Figures xi List of Symbols xviii ... Comparisons of the sloshing free surface between the tank without baffles (A-C) and with baffles (a-c) at t = 1.3, 1.4 and 1.5 s 145 xvii List of Symbols a wave amplitude An wave amplitude of the...

Ngày tải lên: 13/09/2015, 20:56

... weevil control will be the focus of this review The properties of insect a-amylases and available inhibitors will be reviewed and issues affecting their speci®city of interaction addressed INSECT ... interacting with catalytic residues from the A and B domains of TMA (Fig [82]) The residue Asp287, one of the catalytic residues of its enzyme, forms a salt bridge directly with Arg7 of AAI The ... The various known modes of a-amylase inhibition A standard colouring scheme is used with enzyme drawn in magenta and inhibitor helix, strand and coil drawn in red, cyan and yellow, respectively...

Ngày tải lên: 21/02/2014, 03:20

Ngày tải lên: 07/08/2014, 17:22

... coefficient of determination 219 Growth Performance and Sperm Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc Table Production performance, Least square means (LSMSE) of PiDu and ... Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc REFERENCES Dang Vu Binh, V.D Ton, N.C Oanh, (2008) Productivity and meat quality of fattened pigs of crossbred formulas LandracexF1(YorkshirexMong ... Carcass composition and meat quality of crossbreds between F1(LandracexYorkshire) sows mated with LandracexDuroc (omega) and PietrainxDuroc (Pidu) Crossbred Boars Journal of Science and Developement...

Ngày tải lên: 28/08/2013, 11:59

... snapshots relative to the volume of total bonds outstanding For Greece, the net open CDS amount to around 3% of their outstanding sovereign debt and for Portugal and Ireland around 7% This magnitude ... ANALYSIS OF EURO AREA SOVEREIGN CDS AND THEIR RELATION WITH GOVERNMENT BONDS by Alessandro Fontana and Martin Scheicher NOTE: This Working Paper should not be reported as representing the views of ... instances of unchanged prices 2.3 The concept of the ‘basis’ between CDS and bonds In general, both sovereign CDS and government bonds offer investors exposure to the risk and return of sovereign...

Ngày tải lên: 16/02/2014, 02:20

... overexpressed in solid and wet cancer types [10] Mutations and amplification of the androgen receptor gene, without loss of gene expression, play a key role in the development of advanced, androgen-independent ... most often occurring in short stretches of CpG-rich regions known as CpG islands Such regions are about 0.5–2 kb long and can be found in the 5¢-region of approximately 60% of genes, near to their ... on Ser172 of H1.4 For H1.2 and H1.3, the work of Wisniewski et al [43] agrees with that of Garcia et al [42], noting that in Table I of the former paper the phosphorylation on Thr173 of H1.2 is...

Ngày tải lên: 18/02/2014, 08:20

... understanding and will be the focus and repeating theme throughout the text A sound understanding of cardiac physiology begins with basic understanding of cardiac anatomy and of the physiology of ... composed of bundles of myosin strands with each strand having a tail, a hinge and a head region The tail regions bind to each other in the central portion of the filament and the Page of 23 strands ... of this manuscript is to provide a detailed understanding of the heart as a muscular pump and of the interaction between the heart and the vasculature The concepts of contractility, preload and...

Ngày tải lên: 22/02/2014, 09:20

... oxidation of retinol to retinal Retinol and all-trans-retinal were extracted with solid phase C-18 columns (Varian Inc.) The columns were washed with mL of methanol and equilibrated with mL of acetonitrile/water ... washed with mL of acetonitrile/water (60 : 40, v/v), mL of ethanol/water (60 : 40, v/v) and 10 mL of methanol/water (90 : 10, v/v) Retinyl palmitate was eluted with mL of methanol Purity of the sample ... of each nucleotide and 2.5 U of Amplitaq The rat liver cDNA was denatured at 95 °C for and amplified using a DNA Thermal Cycler (Perkin-Elmer) with 35 cycles of 95 °C for min, 58 °C for 30 s and...

Ngày tải lên: 08/03/2014, 10:20

... 537 CPSA of Gelatinase B, Collagen and Their Interaction Fig Characterization of single MMPs according to their structural differences their interaction by using chronopotentiometric ... KGaA, Weinheim 539 CPSA of Gelatinase B, Collagen and Their Interaction Fig Dependences of MMP-9 (1 ng/mL) peak height on A) pH of acetate buffer, B) stripping current and C) and inset, MMP-9 concentration ... does Collagen was dissolved with 9% HCl (m/m) and used in the following experiments 3.2 Collagen Modified HMDE 3.3 Interaction of MMP-9 with Collagen Preparation of standard collagen solutions...

Ngày tải lên: 21/03/2014, 12:18

... profiles and with similar (p > 0.05) values of BS and hardness to textural profile 1, and similar values (p > 0.05) of cohesiveness and springiness to profile 3, and intermediate values of adhesiveness ... times (January, May and October) maintaining brands and meat sausage type At every time of purchase, eight samples were of chopped (CH), nine of mortadella (MT), and seven of galantines (GL) Chopped ... mean of each sample and the standard deviation (SD) of the mean Results and discussion 3.1 Physico-chemical analysis Dry matter, fat content (% dry matter), water activity (aw) and pH values of...

Ngày tải lên: 05/05/2014, 08:43

... incident cases of hand or knee OA as compared with women without OA Of the markers we studied, NTx appears to exhibit the greatest discrimination between participants with and those without BMLs ... Heart Study Offspring cohort included surviving descendants (and spouses of descendants) of the original Framingham Heart Study cohort [25] As part of a study of the inheritance of OA, selected ... Hochberg M: Development of criteria for the classification and reporting of osteoarthritis Classification of osteoarthritis of the knee Diagnostic and Therapeutic Criteria Committee of the American Rheumatism...

Ngày tải lên: 09/08/2014, 13:21