lipospheres as delivery systems for peptides and proteins

... cellular uptake and efficient endosomal release Besides their efficiency as pDNA delivery systems, lipid-based formulations were reported as efficient delivery systems for siRNA in vitro and in vivo ... delivery systems for gene therapy of genetic or acquired human diseases has motivated the search for, and utilisation of, safer carrier molecules Non-viral delivery systems such as naked or formulated ... standard and one of the most efficient non-viral systems for nucleic acid delivery Therefore, PEI has been selected as a reference delivery system in this thesis, and the properties of PEI-based...

Ngày tải lên: 23/08/2015, 17:29

... chemical and physical properties and sources Maltitol (2) Palantinose and tea polyphenol (1) Maltitol and palantinose and tea polyphenol (1) Maltitol and palantinose and erythritol and tea polyphenol ... liquid form and are usually prescribed by a physician for speciÞc diseases or disorders Medical foods are primarily intended for patients in hospitals or for individuals with rare diseases and can ... Inhibits postprandial increase of serum triglyceride levels and prevents fat accumulation and for people with high cholesterol levels (4) A Mixed chemical and physical properties and sources D-Acyl-glycerol...

Ngày tải lên: 25/10/2013, 21:20

... identified for both breast and prostate cancer, for example In contrast, fewer numbers of skin metastases than expected based on blood-flow patterns were found for osteosarcomas, stomach and testicular ... metastases has formed, constituting prevention and treatment studies, respectively Veterinary animals including dogs are increasingly used to test therapeutics in the metastatic setting, and for ... Increased activity of these enzymes has been observed in tumor formation, and therefore inhibitors of MMPs represent an attractive approach to treat cancer Batimastat, marimastat, and prinomastat...

Ngày tải lên: 11/09/2015, 09:58

... reduced and denatured RNaseA, was examined The specific activities of GmPDIL-1 and GmPDIL-2 were 472 and 300 mmol RNaseAÆmin)1Æmol)1, respectively (Fig 2A) Several mammalian and yeast PDI family proteins ... Biotech, Kyoto, Japan) RNaseA refolding assay PDI activity was assayed by the measurement of RNase activity produced through the regeneration of the active form from reduced RNaseA as described previously ... proteins encoded by different genes Therefore, it seems likely that the doublet band may be the 63 kDa PDI and GmPDIL-1 GmPDIL-1, GmPDIS-1 and GmPDIM mRNAs, but not GmPDIL-2 and GmPDIS-2 mRNAs,...

Ngày tải lên: 07/03/2014, 05:20

... the translocation signal and thus will yield only cytoplasm-associated recombinant proteins Selection of vector pMAL-c2x for expression of LASV NP, GP1, and GP proteins was determined by two critical ... indicated that LASV mAb mix was well suited for detecting native and denatured LASV proteins, respectively (data not shown) Rabbit anti-MBP polyclonal antibody was purchased from New England BioLabs ... conditions: 98°C for one 15 second (sec) cycle and then 35 repeated cycles of 98°C for sec, 59°C for 10 sec, and 72°C for 15 sec, followed by a final extension at 72°C for minutes (min) LASV NP was amplified...

Ngày tải lên: 20/06/2014, 01:20

... achieving sustained disease-modifying antirheumatic drug-free remission was highest (11.6%) for anti-CCP2 and varied between 4.7 and 6.0% for anti-CCP3, antiMCV and RF [17] Again, performing two ACPA ... request of the Committee for the Standardization of Autoantibodies in Rheumatic and Related Diseases, the Center for Disease Control and Prevention (Atlanta, GA, USA) has prepared a lyophilized ... to have a favorable effect on the course of the disease Early and accurate diagnosis has therefore become increasingly important For several reasons, the presence of anti-CCP antibodies is a great...

Ngày tải lên: 12/08/2014, 11:22

... tri-phasic burstlike profile that was absent in SBF SBF-soaked scaffolds showed sustained release of the growth factors and total release was not achieved, whereas total release was realized for ... PCL-based honeycomb scaffolds as drug delivery systems for rhBMP-2 Biomaterials 2005; 26(17): 3739-3748 B Rai, S.H Teoh and K.H Ho An in vitro evaluation of PCL-TCP composites as delivery systems ... serve as effective protein delivery systems for the repair 28 of bone tissue The merit of the work was that the PCL-based scaffolds could serve not only as a template for bone growth but also as...

Ngày tải lên: 15/09/2015, 17:10

... equality and empowering women Task Force on Child Health and Maternal Health Who’s got the power? Transforming health systems for women and children Task Force on HIV/AIDS, Malaria, TB, and Access ... targets, and indicators for child health and maternal health Task force approach to health systems 13 Proposed targets and indicators for the child health and maternal health Goals 17 1.1 Task force ... Education and Gender Equality Taking action: achieving gender equality and empowering women Task Force on Child Health and Maternal Health Who’s got the power? Transforming health systems for women and...

Ngày tải lên: 12/02/2014, 23:20

... analysis of variance was done for DM yield, stem proportion and number of stems m-2, content of crude fibre, ash and silica as well as for pulping characteristics Harvest year was used as a repeated ... and production costs): MTT, University of Helsinki and Work Efficiency Association 11 AGRICULTURAL AND FOOD SCIENCE IN FINLAND Saijonkari-Pahkala, K Non-wood plants as raw material for pulp and ... such as grasses and leaf fibre plants, belong to the class Monocotyledonae and bast fibre and fruit fibre plants are dicotyledons (IlvessaloPfäffli 1995) Promising new non-wood species for fibre...

Ngày tải lên: 22/02/2014, 09:20

... equality and empowering women Task Force on Child Health and Maternal Health Who’s got the power? Transforming health systems for women and children Task Force on HIV/AIDS, Malaria, TB, and Access ... targets, and indicators for child health and maternal health Task force approach to health systems 13 Proposed targets and indicators for the child health and maternal health Goals 17 1.1 Task force ... Education and Gender Equality Taking action: achieving gender equality and empowering women Task Force on Child Health and Maternal Health Who’s got the power? Transforming health systems for women and...

Ngày tải lên: 05/03/2014, 10:20

... designated as feasible candidates for drug delivery systems, anti-cancer agents and vaccine immunotherapy For example, DNA vaccine delivery to APCs has been facilitated by microencapsulation of plasmid ... class I and class II responses [84] Consequently, VLPs have great potential as epitope display systems for other diseases Human papillomavirus-like particles (HPV VLP) are a candidate vaccine for ... potent and long-lasting immune responses and advances in materials science and technology that can be used to develop more effective and targeted delivery systems [113] Non-invasive techniques for...

Ngày tải lên: 06/03/2014, 02:21

... Translating Genomic-Based Research for Health were crucial to the planning and conduct of the workshop, Systems for Research and Evaluation for Translating Genome-Based Discoveries for Health Federal ... question was asked as to whether the appeals process mentioned by Teutsch would address passive challenges, such as a need for change identified as a result of horizon scanning, as well as active ... disease With the advent of genome-wide association (GWA) studies, however, numerous associations between specific gene loci and complex diseases have been identified, for example for breast cancer,...

Ngày tải lên: 15/03/2014, 15:20

... Transforming Health Systems for Women and Children Summary version of the report of the Task Force on Child Health and Maternal Health New York, USA For more information about the Task Force ... public and private, formal and informal, forprofit and not -for- profit, allopathic, and indigenous It also includes such mechanisms as insurance by which the system is financed, as well as the ... safely, and wash hands after defecation, before preparing meals, and before feeding children Take children as scheduled to complete a full course of immunizations (BCG, DPT, OPV, and measles) before...

Ngày tải lên: 22/03/2014, 10:20

... responsible for the structural and functional integrity of cells They form the chemical basis of cellular functions that are based on highly specific molecular recognition and binding, and are involved as ... be efficient as Na-protectors for general applications Nonetheless, they are useful as protecting groups for side-chain functions as well as for the protection of the a-amino group for the synthesis ... concentration (2% v/v in DMF for as against 20% v/v in DMF for about 30 for Fmoc removal), thus leading to minimum or no formation of side-products such as aspartimide that arise due to base-catalyzed reactions...

Ngày tải lên: 02/04/2014, 15:59

... this reason, pH gradients are not as common as salt gradients as a means to elute proteins in ion-exchange chromatography (4) In contrast, steps in pH are easier to control and reproduce, and for ... best suited for low molecular weight solutes such as nucleotides and small peptides, whereas larger pore sizes (300 Å or greater) will work better for larger peptides, proteins, and nucleic acids ... Zhou, N E., Mant, C T., Kirkland J J., and Hodges R S (1991) Comparison of silica-based cyanopropyl and octyl reversed phase packings for the separation of peptides and proteins J Chromatogr 548,...

Ngày tải lên: 11/04/2014, 09:46

... calsequestrin and calretinin;9 enzymes like phospholipases10 and calmodulin11) and give rise to complex RD systems synchronizing intracellular and intercellular events as diverse as secretion ... physical basis of RD and the theoretical tools that can be used to model it In these and other chapters, new concepts are derived from the basics and assume only rudimentary knowledge of chemistry and ... United Kingdom For details of our global editorial offices, for customer services and for information about how to apply for permission to reuse the copyright material in this book please see our...

Ngày tải lên: 04/06/2014, 15:05

... Among the problems are those associated with classified waste disposal, ammunition, plant wastes, chemical warfare wastes, hazardous toxic waste, and radioactive wastes Each will require a consultant ... Guide for Architects; and handbooks prepared by government agencies such as the Naval Facilities Engineering Command's "Economic Analysis Handbook”, NAVFAC P-442 Clarification of the basic standards ... Manual (DOD 4270 1-M) Standards for the conduct of all economic studies by and for the Department of the Army and the Department of the Air Force are contained in AR 11-28 and AFR 178-1, respectively...

Ngày tải lên: 20/06/2014, 17:20

... equipment and boiler b Fuel type (1) Gaseous fuels Gaseous fuels burn more readily and completely than other fuels Because they are in molecular form, they are easily mixed with the air required for ... Technical tasks The principal technical tasks that are required for the permitting effort in most cases may be summarized as follows: (1) Engineering studies of expected emission rates and the control ... fuse the ash into slag which is drained from the furnace bottom Tangentially fired furnaces and slag-tap furnaces decrease the amount of fuel ash emitted as particulates with an increase in NOx...

Ngày tải lên: 20/06/2014, 17:20

... Protection Agency Regulations on Standards of Performance for New Stationary Sources, 40 CFR 60 and subsequent revisions The techniques are listed in table 5-1 standards For the determination of possible ... knowledgeable and experienced in meteorology and air quality testing are needed to conduct and evaluate air-quality measurements b Sampling technique The criteria for instrumentation, calibration, and ... Monitoring Reference and Equivalent Methods See table 5-2 (1) 5-3 Meteorological and ambient air measurement a Measurements Air quality measurements are used to trace emission sources and determine...

Ngày tải lên: 20/06/2014, 17:20

... Cyclone performance a Collection efficiency and pressure drop For any given cyclone it is desirable to have as high a collection efficiency and as low a pressure drop as possible Unfortunately, ... cone length, and the ratio of body diameter to gas outlet diameter Decreased efficiency is caused by an increase in gas viscosity, gas density, cyclone diameter; gas outlet diameter; and inlet widths ... consisting of plastic, glass, or marble spheres where gas and liquid can mix intimately (figure 7-3) A cylindrical shell holds a perforated plate on which the movable packing is placed Gas passes upward...

Ngày tải lên: 20/06/2014, 17:20