liposome dna complexes and their interaction with cells

... known modes of a-amylase inhibition A standard colouring scheme is used with enzyme drawn in magenta and inhibitor helix, strand and coil drawn in red, cyan and yellow, respectively The calcium ... activity PPA and HSA 0.19 T aestivum PPA and HSA 0.53 T aestivum HSA and PPA (low) 0.28 WRP25 T aestivum T aestivum PPA and HSA None WRP26 T aestivum None WRP27 T aestivum None 1,2 and BIII S cereale ... 1) and forms a separated folding unit, exclusively made of b sheet Eight of the 10 strands fold into a b sandwich structure with ÔGreek keyÕ topology The conservation of the interface of A and...

Ngày tải lên: 21/02/2014, 03:20

... maps, with the o and coot programs [36,37] The final refinement was performed with refmac from the ccp4 suite [32], with TLS parameterization Both cns and refmac used the same Rfree set (randomly ... dithiothreitol and mm EDTA, and purified further by anion exchange chromatography with a Mono Q column (Amersham Biosciences; 20 mm Tris ⁄ HCl, pH 7.5, containing 10 mm dithiothreitol and mm EDTA, with ... [16] Additionally, the bands containing the RI–RNase A or RI–SGRSGRSG-RATE complexes (Fig 4, lanes and 4) were excised and subjected to SDS ⁄ PAGE, in which the noncovalent complexes dissociate...

Ngày tải lên: 22/03/2014, 16:21

... and their abiotic analogues) Generally, single-stranded abiotics are unnatural backbones that mimic secondary structures, such as folding and helices, while multi-stranded foldamers ... flexibility of the linkers and various nonconvalent interactions that enforce discrete folded conformation Considering these advantages, single-stranded foldamers with various abiotic backbones ... solvophobic interaction, 7 hydrogen-binding interactions8 and metal coordination.9 However, the challenges in designing synthetic folding oligomers exist in terms of folding stability, tunability, and...

Ngày tải lên: 10/09/2015, 15:49

... stacking and van der Waals’ interactions provide the driving forces for the observed formation of columns With their persistent shapes, tunable sizes and tendency to aggregate into column- and channel-like ... between axis ac and plane of the molecules, all the other three dimers (2a, 2c and 2d) are stacked along axis a with angles of 90º, 53º and 70º, respectively, between axis a and plane of the ... from 3a and generated by breaking H-bond of Oa···Hb (1A & 1B), Hb···Oc (2A & 2B), Oc···Hd (3A & 3B) and Hd···Oe (4A & 4B), and by breaking H-bonds of both Oa···Hb and Hb···Oc (5A & 5B), and both...

Ngày tải lên: 10/09/2015, 15:49

... slow evaporation of 5a and 6a in mixed solvents containing hexane and chloroform 91 (1:1 v/v) for 5a and hexane and dichloromethane (1:1 v/v) for 6a at room temperature Their crystal structures ... contact between proton and proton 24 in 6c (Figures 3.5d and 3.5e), we decided to replace the methyl side chain at the nitro end with an isopropyloxy side chain to generate 5b and with an octyloxy ... protons and their adjacent interior methoxy protons, (f) Chemical structure of 6b More NOESY, ROESY and TOXSY studies of 6b were demonstrated in Figure 3.8 and Figure 3.9 Figure 3.8c and 3.8d...

Ngày tải lên: 10/09/2015, 15:49

... mL) and the mixture was heated under reflux for h The solvent was removed in vacuo and anhydrous ether (2 x mL) was added and removed again The solvent was then removed in vacuo and saturated with ... The mixture was heated at 50 0C overnight and then quenched 126 with water (4 ml) The aqueous layer was neutralized with 1M KHSO4 (0.4ml) and extracted with DCM (2 x mL) Removal of CH2Cl2 solvent ... (1.5 mL) and the mixture was heated under reflux for h The solvent was removed in vacuo and anhydrous ether (2 x 30 mL) was added and removed again The solvent was then removed in vacuo and saturated...

Ngày tải lên: 10/09/2015, 15:49

... been used very often in quadruplex-targeting ligands to enable the ligand to efficiently interact with the grooves and loops of quadruplex DNA and the negatively charged backbone phosphates In ... telo23 DNA sequence.33 In the study of G-quadruplex structure, effective assays are required for studying the G-quadruplex formation and for determining the interaction between ligands and G-quadruplexes ... inhibit the action of the DNA polymerase.41 The single-stranded DNA could be induced into a G-quadruplex structure that blocks hybridization with a complementary strand overlapping the last G...

Ngày tải lên: 10/09/2015, 15:49

... (15-mer) and TBA2 (29-mer) and their analogues were chemically coupled to AuNPs via the formation of Au-S bond Table 6.1 lists all the DNA- modified AuNPs AuNPs heavily loaded with linear DNA strands ... both Au-T6 and thrombin Our earlier observation and discussion in the duplex approach shows that the interaction of TBA with its complementary DNA strand is stronger than that between TBA and thrombin ... shorter complimentary DNA strand containing a region of about 12-20 nucleotides complementary to DNA apamer and (3) gold nanoparticles (13 nm in diameter) functionalized with DNAs that enable the...

Ngày tải lên: 10/09/2015, 15:49

... oligomers and TBA2: Au-TBA2 with (a) thrombin only, (b) with both thrombin and 1, (c) with both thrombin and 9a, (d) with both thrombin and 10b, (e) with both thrombin and 11a, (f) with thrombin and ... due to the nonspecific interactions between lysozyme and DNAs attached to the AuNPs With the addition of various pentamers into the solution containing DNA- modified AuNPs and lysozyme, AuNPs aggregation ... presence of thrombin and 10a (2 μM) 0.7 Absorbance 0.6 0.5 0.4 0.3 0.2 300 400 500 600 700 800 Wavelength (nm) Figure 7.4 UV-vis spectra of Au-TBA with both thrombin and 10a and with thrombin only...

Ngày tải lên: 10/09/2015, 15:49

... reaction, and γ ratio of the specific heat of the gas ρ1 , p1 , and u1 represent the density, pressure, and velocity of the unburnt gas, and ρ , p2 , and u2 represent the density, pressure, and velocity ... Structure with one transverse wave with the mesh size of 0.05mm 146 Fig 5.30 Structures with One Transverse Wave with the mesh size of 0.025mm 147 Fig 5.31 Formation of Triple-wave Configuration with ... Pressure contours with different mesh size at time = 871µs 143 Fig 5.27 Structure with one transverse wave with the mesh size of 0.1mm 144 Fig 5.28 Structure with one transverse wave with the mesh...

Ngày tải lên: 11/09/2015, 16:07

... On the other hand, Li & Fleming (1997) solved the Laplace equation in σ-coordinate with the use of FDM Frandsen & Borthwick (2003) and Frandsen (2004) proposed another FDM model with σ-coordinate ... compared with the well-known MacCamy and Fuchs closed form analytical solution Good agreements are obtained Finally, the breaking wave interaction with a spar platform in deep ocean is demonstrated and ... Hanbin, Dr Fernando and Dr Anuja, for their friendship and valuable discussion during the study Special thanks go to Mr Cheng Yonggang for helping me to solve the problems of CAD and other softwares...

Ngày tải lên: 13/09/2015, 20:56

... FUNCTIONALIZED SUBSTRATES AND THEIR INTERACTIONS WITH BIOMOLECULES AND CELLS CEN LIAN (B.Eng., ECUST) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ... supervisors, Prof K G Neoh and Prof E T Kang, for their heartfelt guidance, invaluable suggestions and profound discussion throughout this work, for sharing with me their enthusiasm and active research ... (b) PPY surface grafted with vol.% HEA and subsequently silanized with vol.% ATS in dioxane; (c) PPY film graft-copolymerized with vol.% HEA and subsequently silanized with vol.% ATS in dioxane...

Ngày tải lên: 16/09/2015, 17:12

... Growth Performance and Sperm Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc Kawecka et al (2008), Wysokinska et al (2009), Pokrywka and Tereszkiewicz (2011) and Luc et al (2011; ... Carcass composition and meat quality of crossbreds between F1(LandracexYorkshire) sows mated with LandracexDuroc (omega) and PietrainxDuroc (Pidu) Crossbred Boars Journal of Science and Developement ... means with differing letters in each row within an effect differ (P

Ngày tải lên: 28/08/2013, 11:59

... Netherlands and Austria is quite substantial and is approximately - 0.2; for France and Belgium it is smaller, namely - 0.005 For Italy, Ireland, Spain, Portugal and Greece is not significant and ... relative to the volume of total bonds outstanding For Greece, the net open CDS amount to around 3% of their outstanding sovereign debt and for Portugal and Ireland around 7% This magnitude is in contrast ... 2010 or Bharot and Guo, 2010) In contrast, we have documented that Euro area sovereigns with the temporary exception of Ireland, Greece and Portugal have a positive basis 16 14 Gorton and Metrick...

Ngày tải lên: 16/02/2014, 02:20

... in cultured cells [43] It is possible to come up with a list of PTMs that are either absent in MCF7 cells and present in HeLa cells, or present in MCF7 cells but missing in HeLa cells (Table ... patterns in the linker histones with particular functions, and that unique patterns of PTMs exist for diseased cells when compared with normal cells, and between cells of different types FEBS Journal ... linker histones mouse cells [57] A proportion of murine embryonic stem cells, embryonic fibroblasts and embryonic carcinoma cells were grown in standard cell growth medium, with the remainder having...

Ngày tải lên: 18/02/2014, 08:20

... understanding of the heart as a muscular pump and of the interaction between the heart and the vasculature The concepts of contractility, preload and afterload are paramount to this understanding and ... bundles of myosin strands with each strand having a tail, a hinge and a head region The tail regions bind to each other in the central portion of the filament and the Page of 23 strands are aligned ... troponin and tropomyosin Tropomyosin is a thin protein strand that sits on the actin strand and, under normal resting conditions, covers the actin-myosin binding site thus inhibiting their interaction...

Ngày tải lên: 22/02/2014, 09:20

... activity and the activity bands for corresponding peaks are labeled with arrows In this gel, peak appeared as a doublet (band a and b) Each peak was further separated on nondenaturing PAGE and the bands ... the 5¢- and 3¢-RACE products were cloned and sequenced The complete cDNA for this carboxylesterase was cloned by amplifying rat liver cDNA with the primers 5¢-CTGAGATTCAACCATGCCTTTGGC-3¢ and 5¢-TTG ... nucleotide and 2.5 U of Amplitaq The rat liver cDNA was denatured at 95 °C for and amplified using a DNA Thermal Cycler (Perkin-Elmer) with 35 cycles of 95 °C for min, 58 °C for 30 s and 72 °C...

Ngày tải lên: 08/03/2014, 10:20

... B, Collagen and Their Interaction Fig Characterization of single MMPs according to their structural differences their interaction by using chronopotentiometric stripping analysis with adsorptive ... Electrochemical measurements were performed with AUTOLAB Analyzer (EcoChemie, Netherlands) connected to VA-Stand 663 (Metrohm, Switzerland), using a standard cell with three electrodes A hanging mercury ... collagen, MMP-9 and collagen after interaction with MMP-9 measured by AdTS CPSA (interaction time: 30 s) D) Height of CPSA peaks of collagen (0.5 or mg/mL) after interaction with MMP-9 (0.5 or...

Ngày tải lên: 21/03/2014, 12:18

... combining to enable devices and systems with potential global effects on individual and public health and safety; economic, social and political systems; and business and commerce The emerging ... advances and products in biomedical engineering and technology of organic and artificial tissues, organs, and materials Organic Tissues and Organs Advances in tissue and organ engineering and repair ... stays and cost and increase effectiveness New surgical tools and techniques and new materials and designs for vesicle and tissue support will likely continue to reduce surgical invasiveness and...

Ngày tải lên: 29/03/2014, 14:20

... mortadella and galantines) from different commercial brands were purchased in retail shops in Spain The 24 samples were 691 bought three times (January, May and October) maintaining brands and meat ... of mortadella (MT), and seven of galantines (GL) Chopped is a heat-cured meat sausage manufactured with a mixture of ham chunks and trimmings and seasonings, ground together and then packaged into ... $70% fine emulsion with flat strip meat (0.5 Â 1.5 cm) and coarse fat $55% fine emulsion with coarse meat (1–1.5 cm2) $80% fine emulsion with coarse meat (0.5–2 cm2) $60% fine emulsion with irregular...

Ngày tải lên: 05/05/2014, 08:43