liposomal delivery of protein and peptide drugs

... Peptide /protein drug delivery 15 2.2.1 Structural aspect of protein 15 2.2.2 Challenges in peptide /protein drug delivery 17 2.2.3 Approaches for delivery of peptide /protein ... for delivery of peptide /protein drugs 2.2.3.1 Parenteral delivery Due to the poor absorption and low bioavailability by all non-parenteral routes for effective systemic delivery of peptide /protein ... NANOPARTICLES OF BIODEGRADABLE POLYMERS FOR CONTROLLED AND TARGETED DELIVERY OF PROTEIN DRUGS SMALL AND MOLECULE DRUGS BY LEE SIE HUEY (B.Sc (Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER...

Ngày tải lên: 26/11/2015, 22:41

... backbone and the nature of antigen presentation and T cell priming from both the Page 10 of 12 IalB and Omp25 candidate vaccines may be useful in guiding further development of these vaccines and ... 3), of B melitensis 16 M Upon completion of all three experiments each individual study and the combined data was analysed to enable comparison of each of the various vaccine candidates and protocols ... vaccination of livestock Different routes of delivery have been investigated to improve DNA uptake and in vivo expression including techniques such as in-vivo electroporation [19,20] and the use of microparticulate...

Ngày tải lên: 14/08/2014, 19:22

... otherwise slow up the rate of sample electrophoresis and also spoil the resolution of protein bands As mentioned earlier, protein bands in this system are often slightly misshapen, and this problem is ... wells at the very ends of the row of wells, for samples in these often suffer distortion during the electrophoresis 14 Be wary of the dangers of electric shock and of fire, and of the neurotoxic acrylamide ... aggregation and precipitation of proteins Also, the pH of the stacking gel (pH 6.8) may affect the activity of the protein of interest If this is thought to be a problem (e.g., the protein cannot...

Ngày tải lên: 10/04/2014, 11:03

... be capable of the analysis of large underivatized peptides (8), and, soon after, of proteins, by the demonstration of the first mass spectrum of insulin in 1982 (9) and of a number of snake toxms ... Separation and solvatton of analyte molecules m the solid phase, avoiding agglomeratron of analyte material, Uptake of laser energy used for desorptton and ronizatlon of matrix and analyte molecules, and ... of the ammo acid sequence of peptides Nature 211,361-366 Biemann, K and Vetter, W (1960) Separation of peptide derivatives by gas chromatography combmed with mass spectrometrtc determmation of...

Ngày tải lên: 11/04/2014, 10:11

... a single injection of mg/kg liposomal DXM-P The lower doses of 1.6 or 0.4 mg/kg of the liposomal form resulted in less than 4% reduction of body weight Presence of free and liposomal dexamethasone ... Effects of liposomal and free dexamethasone phosphate on blood glucose, liver enzymes and haematology Effects of liposomal and free dexamethasone phosphate on blood glucose, liver enzymes and haematology ... X1 X21 X25-35 10 11 12 13 14 Liposomal DXM-P 1x i.v treatment Collection of blood days after start and end of treatment Collection of blood days after start and end of treatment Study design Study...

Ngày tải lên: 12/08/2014, 11:22

... mixture of oxidized tetracysteines in the protein sample FlAsH fluorescence of the labeled protein is also observed (right) and shows a trend of increased fluorescence, relative to the amount of protein ... oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding Proceedings of the National Academy of Sciences of the United States of America 100, 15936-15941 (2003) 10 Andresen,M., ... xii ABSTRACT OF THE DISSERATION Optimization of protein and RNA detection methodologies and a new approach for manipulating protein activity in living cells by Brent R Martin Doctor of Philosophy...

Ngày tải lên: 13/11/2014, 10:46

... Materials and Methods 3.8 Zeta Potential and Size of the Complexes Appropriate amount of peptide at desired ratios in 250 µl of 5% glucose was added in a dropwise manner to 20 µg of DNA in 250 µl of ... utilized for the delivery of nucleic acids in gene therapy, namely that of viral vectors and non-viral vectors The natural ability of viruses to infect host cells offers an existing class of vectors ... nuclear transport; and vector unpacking and translation (Fig 1-2) Any of these steps can contribute to the efficiency of gene delivery by polyplexes Therefore, a thorough understanding of these steps...

Ngày tải lên: 27/11/2015, 11:11

... undertaken and involves the recovery of public assets, the breach the bank and tax privacies, and the seizure and forfeiture of goods and assets involved Furthermore, anticipatory alienation of assets ... encounter, combat, and address this kind of criminality in a proper and unified way One of the political impacts of the implementation of such courts is the motivation of formal institutions of power (e.g., ... federal, state, and local levels A sense of impunity bred by a slow and inefficient judiciary and judicial system also hinders the reduction of corruption The presumption of innocence and the legal...

Ngày tải lên: 26/08/2016, 07:36

... also proved that majority of fusion protein was present in inclusion body (Fig 2B) Figure Maps of N-terminal His-tagged gAd-GLP-1-A fusion protein and gAd-GLP-1-A fusion protein (A) N-terminal His-tagged ... the C-terminus of GLP-1-A Compared with native GLP-1, the fusion protein has a modified site and larger molecular size, and may circumvent the rapid inactivation and renal clearance of GLP-1 Studies ... part of fusion protein or both of them In other words, we need to know whether each part of the fusion protein play glucose-lowering effect separately We also need to know whether the half-life of...

Ngày tải lên: 25/10/2012, 11:10

... PHM, GRF and GIP GLP-1 and GLP-2 are similar peptides, with molecular masses of 3299 Da and 3768 Da, respectively Both peptides have His-Ala as the N-terminal dipeptide, and DPP4 cleavage of these ... capable of the same cleavage event, producing peptides of 3090 Da and 3558 Da for GLP-1 and GLP-2, respectively Both peptides were inefficient FAP substrates, with half-lives of 22 h and 19 h, ... with peptide chain length [43] However, in contrast to this, in the case of GRF, the rate of DPP4 hydrolysis of longer peptides (44 amino acids) is higher than that of shorter peptides (three and...

Ngày tải lên: 14/03/2014, 23:20

... ecnabrosbA ecnabrosbA ASILE editpep C2 fo noitadilav lacigolores rof selpmas ares enivoB DMF TIKEHC elba T 913 kcolb ot ruoh tsael ta rof Co73 ta llew rep )T-SBP ni ASB %1 ,enitaleg %01( reffub gnikcolb ... aeroK ni kaerbtuo DMF eht gnirud elttac 32 morf detcelloc saw puorG spuorg ruof otni dedivid erew ares enivob xis-ytrof ,stik rehto htiw ASILE editpep C2 eht fo stluser tset eht erapmoc oT )KU ... yllatnemirepxe ,evitisop dleif sa deifissalc erew ares eseht dna noitaulave rof deraperp erew ares enivob thgie-ytroF )1 giF( noitcefni ot roirp lamina emas eht morf mures evitagen eno dna IPD...

Ngày tải lên: 07/08/2014, 18:21

... amount of drugs bound to NPs and the type of interaction –between drugs and nanoparticles depend on the chemical structure of the drug, chemical structure of the polymer and the conditions of drug ... number of biomolecules, drugs, genes and vaccines to the site of interest in-vivo During the 1980’s and 1990’s several drug delivery systems were developed to improve the efficiency of drugs and ... oral drug delivery vehicles Oral delivery of drugs using nanoparticles has been shown to be far superior to the delivery of free drugs in terms of bioavialability, residence time, and biodistribution...

Ngày tải lên: 11/08/2014, 08:20

... to conceptualize and analyze the entire regulation and signaling networks of different normal and cancer cells The understanding of network system of gene expression profiles and interaction between ... 1.4.8 Protein and peptide delivery using nanoparticles 1.4.8.1 Protein loading into nanoparticles Polymers used for in protein/ drug 1.4.8.2 delivery systems 1.5 Objectives and scope of the research ... 137 137 v 6.2.5 6.2.6 6.2.7 Cellular delivery and uptake of Herceptin Co -delivery of Pac and Herceptin to human breast cancer cell lines Targeted delivery of drug-loaded nanoparticle/Herceptin...

Ngày tải lên: 30/09/2015, 05:59

... process of C -peptide and analyzed the formation of oligomers and their secondary structure by complementary methods, including the detection of C -peptide oligomers by PAGE electrophoresis and spectroscopic ... time and analyzed under native conditions Oligomer distribution of 100 lM proinsulin C -peptide in the presence of divalent Ca2+ and Mg2+ ions under native conditions (B), and of 100 lM C -peptide ... oligomerization of C -peptide, and does not alter other conditions In summary, C -peptide is predominantly a monomer in buffer solutions at low pH and, in the presence of modest amounts of SDS, the peptide...

Ngày tải lên: 18/02/2014, 04:20

... concentrations Sequence analysis and modelling of LlChi18A and LlCBP33A Cloning and purication of LlChi18A and LlCBP33A The gene fragments coding for the mature proteins of LlChi18A and LlCBP33A were successfully ... chitinase and CBP, as the N-terminus of the latter protein should be free of non-native amino acids after removal of the afnity tag (because amino acid one of the mature protein is conserved and seems ... containing 100 lm of (GlcNAc)14 was analysed at the start, in the middle and at the end of each series of samples The resulting average values of the standards (displaying standard deviations of < 5%)...

Ngày tải lên: 18/02/2014, 08:20

... mRNA and also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP and the level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA ... UTR of IL-6 mRNA [4,17] The presence of such a sequence may account for the degra- MCPIP protein as an RNase dation of both types of IL-1b transcripts, with and without AREs The influence of MCPIP ... also proteins involved in other cellular processes, such as actin Further studies characterizing proteins interacting with MCPIP will clarify the dynamics and features of this protein Role of MCPIP...

Ngày tải lên: 18/02/2014, 14:20

... translocase of outer membrane (TOM) receptors (TOM20, TOM22 and TOM70), which enables the translocation of proteins through the outer membrane and the inner membrane channel-forming proteins, TOM40 and ... [23] The precise nature of CYP proteins targeted to mitochondria and the conservation of targeting mechanism is important for understanding the evolution and regulation of bimodal targeting Our ... expression of genes to study both gene function and metabolism The protein translocation machineries of both the mitochondria and microsomes are highly conserved among mammals and yeast [24,25] As protein...

Ngày tải lên: 18/02/2014, 16:20

... Gevaert et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... features of ATP-binding proteins As expected, the majority of FSBA-labeled Jurkat proteins were known binders of small nucleotides, cofactors, or DNA and RNA molecules However, several proteins and ... isolate, for instance, phosphopeptides [24], N-glycosylated peptides 6278 [25], ubiquitinated peptides [26], and N-terminal peptides [27] COFRADIC as a peptide- sorting tool Our peptide- centric proteome...

Ngày tải lên: 18/02/2014, 16:20

... banding patterns and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of ... isoform (m) of PrPC were quantified and calculated as percentages of the total signal The glycoforms of the protein bands were analyzed as arithmetic means of separate gel runs The number of gel runs ... Calculation of the banding patterns of 10 gels using antibody SAF34 gave an SE value of 2.1 for the di-glycosylated isoform, 1.6 for the mono-glycosylated band and 3.2 for the nonglycosylated protein; ...

Ngày tải lên: 19/02/2014, 02:20

... ephrinB2(301–333)-pY304 peptide in the absence of the GSTPDZ protein (d) or with 75 lM of the GST-PDZ protein (s) protein We set out to address this question by use of the SH2 domain of Grb4 and the PDZ domain of ... Grb4 and PDZ-RGS3 proteins Activation of reverse signaling through B ephrins leads to clustering and tyrosine phosphorylation of the cytoplasmic tail of B ephrins, and a concomitant recruitment of ... (Fig 1A and Fig 2B) 1728 Z Su et al (Eur J Biochem 271) Ó FEBS 2004 Fig Comparison of amino acid sequences of SH2 domains from Src and the mouse Grb4, and expression and purification of the Grb4...

Ngày tải lên: 19/02/2014, 12:20