... overwhelm cellularantioxidant defenses but also attack cellular structural molecules, leading to oxidative modification of erythrocytes [10] Antioxidants can help in preservation of an adequate antioxidant ... thiobarbituric acidand 1.5 ml 20% acetic acid was prepared 200 μL of RBCs lysate and 1.9 ml distilled water were added to complete the volume of ml After boiling for h, the mixture was cooled, and ml ... fragility and oxidative damage of erythrocytes of zinc-deficient rats J Nutr 1997; 127: 1290–1296 Lajos N, Miki N, Sandor S Protein and non-protein sulfhydryls and disulfides in gastric mucosa and...
... chapters Nutrition and Metabolism: Underlying Mechanisms andClinical Consequences, edited by Christos S Mantzoros, MD is a very welcome addition to the Nutrition and Health Series and fully exemplifies ... reduction of salt, saturated and trans fats and increases in dietary fiber, antioxidants, B vitamins, omega-3 fatty acids, mono-unsaturated fatty acids, calcium, and potassium Examples of food-based ... up-to-date and authoritative review of the major scientific andclinical aspects of the overlapping areas between nutrition andmetabolism I am indebted to all my colleagues, most of them scientists and...
... and to evaluate the influence of the association of rutin to different nanostructures (nanocapsules and nanoemulsions) on its in vitro antioxidantactivityand UV photostability Nanocapsules and ... vitro antioxidantactivity of rutin-loaded nanostructures and ethanolic solution after 30 of UV irradiation 123 120 100 Active release (%) The antioxidantactivity (radical scavenging activity) ... observed at different magnifications AntioxidantActivityand Photostability Study The system for the irradiation of the samples was developed by Carvalho et al [38] and consisted of a 400 W high-pressure...
... GR: Lipoic acid: a multifunctional antioxidant Biofactors 2003, 17:207-213 Jones W, Li X, Qu ZC, Perriott L, Whitesell RR, May JM: Uptake, recycling, andantioxidant actions of alpha -lipoic acid ... 2B and 2C, and Figure 3B–E, TCH carried out the studies in Figure 2A, Figure 3A and Figure 4, TCH conceived of the study, and participated in its design and coordination All authors read and ... HJ: alpha -Lipoic acid as a biological antioxidant Free Radic Biol Med 1995, 19:227-250 Packer L, Tritschler HJ, Wessel K: Neuroprotection by the metabolic antioxidant alpha -lipoic acid Free Radic...
... collection and data analysis, and drafted the manuscript MK and JB contributed to data analysis DF-B, FG, CG, and TP contributed to study design and undertook pathology review DF, MM, JG, and RG ... 8a-d) Association between outcome andclinical variables For each trial, outcome analyses were undertaken using clinicaland pathological data The following clinical variables were considered: ... encountered in clinical practice, and (ii) problems with evaluating types of necrosis in both grade II and grade III tumors produced further refinement of our views on grading and led to the...
... fraction, tidal volume, and respiratory frequency, and of arterial acid base and oxygenation status (SaO2, PaO2, pH, partial pressure of carbon dioxide, haemoglobin, methaemoglobin, and carboxyhaemoglobin) ... oximetry data [14] and simple clinical methods [13], their clinical application seems timely Key messages • The variation in the PaO2/FiO2 ratio with the FiO2 level is scientifically and clinically relevant ... authors contributed to the conception and design of the study SK, KE and CA contributed to the data collection andclinical interpretation of the results DSK, BWS, SA and SER contributed to the mathematical...
... triage and management in emergency settings We present a review of the clinicaland molecular/structural basis of the Hoover's sign and explain how it could be utilized in the bedside and emergent ... the rib cage and lower intercostal interspaces (Figure Panels A and B) This patient had evidence of moderate airway obstruction and elevated residual volumes (Figure Pan- els C and D) There was ... historically and numbers of prescribed drugs were independently associated with the sign [3] Page of (page number not for citation purposes) Clinicaland Molecular Allergy 2008, 6:8 http://www.clinicalmolecularallergy.com/content/6/1/8...
... skeletal muscle succinate dehydrogenase and cytochrome oxidase activities and maximal oxygen uptake with physical activityand inactivity Acta Physiol Scand ; : ệrlander J, Kiessling KH, Ekblom B Time ... a tradition in Scandinavia for an interaction between exercise physiology andclinical medicine and surgery, and it is apparent that both areas have hypotheses, inspiration and possible solutions ... and bone; however, in contrast, its slower metabolism requires perhaps up to months or more before complete tissue recovery from an injury and subsequent inactivity Thus, an injury that demands...
... for eIF5A activity being regulated by Lys47 acetylation Therefore, we substituted Lys47 with three different amino acids, i.e acidic, neutral and basic amino acids The fact that eIF5A activity ... stability andactivity eIF5A has an extended N-terminus and a different C-terminus as compared to the bacterial (EF-P) [18] and archaeal (aIF5A) homologs Recently, the N-terminal extension (amino acids ... DHS and DOHH [53] These findings provide evidence that both the N-terminal and C-terminal domain b-sheet core structures (amino acids 17–82 and 85–146) (Fig 1) are required for the biological activity...
... factor-1 gene promoter activity by AP1 andcellular nucleic acidbinding protein DNA Cell Biol 17, 799–809 Pellizzoni L, Lotti F, Maras B & Pierandrei-Amaldi P (1997) Cellular nucleic acid binding protein ... mobility controls: labelled Comp-CT alone and a completely annealed reaction Samples were taken at 0, 1, 3, 10, and 30 Single-stranded (ss) and double-stranded (ds) species are indicated (B) Bar ... test) protein that binds single-stranded nucleic acids and is able to promote interstrand reannealing of DNA as well as RNA [35,36] hnRNP Al is phosphorylated by CK2 and PKA PKA phosphorylation suppresses...
... nonphosphorylated (N) and phosphorylated (pN) N proteins (lanes and 4) Lanes and are the mock reactions without crosslinker The right-hand panel shows the relative abundance (percentage) of monomer and crosslinked ... phosphatase (New England Biolabs, Beverly, MA, USA) CD spectrometry HeLa and HEK293 cells were grown in DMEM (Gibco, Grand Island, NY, USA) supplemented with 10% fetal calf serum and penicillin ⁄ ... Tai-Huang Huang and Konan Peck for the SARS-CoV N protein cDNAs, plasmids and recombinant proteins, and Chwan-Deng Hsiao and Yi-Wei Chang for CD analysis We thank Ru-Inn Lin and Wei-Lun Chang...
... that the sequenced product and that with peptidase activity were the same The puri®ed enzyme was checked for its phosphatase activity on p-nitrophenyl phosphate and this activity was compared to ... pooled and checked for purity by SDS/PAGE and C4 reversed-phase chromatography using a gradient of 0±60% acetonitrile in 0.075% CF3COOH Fig pH activity pro®le of PAP activity (full line) and PAP ... and activation of acid phosphatase in seminal ¯uid Int J Androl 4, 372±378 10 Meng, T.C & Lin, M.F (1998) Tyrosine phosphorylation of c-ErbB-2 is regulated by the cellular form of prostatic acid...
... showed Ó FEBS 2002 Haloperoxidase activity of phosphatases (Eur J Biochem 269) 2165 relevant levels of acid phosphatase activity On induction, the specific activity of acid phosphatase in both strains ... Haloperoxidase activity of vanadate-substituted acid phosphatases The brominating activity of recombinant PhoN-Sf and PhoN-Se was tested in a phenol red assay After overnight incubation of lM PhoN-Sf and ... vanadate and the activity measured in the MCD assay (A) PhoN-Sf in mM H2O2 and variable concentrations of Br– (B) PhoN-Sf in 300 mM Br– and variable concentrations of H2O2 (C) PhoN-Se in mM H2O2 and...
... transfected with empty vector and cells treated by 50 µM cisplatin for 16 hr were set as (-) and (+) controls Transfected cells were collected at 24 hr and 48 hr after incubation and analyzed by Apoalert™ ... amino acid sequences were analyzed by using Vector NTI9 Result was generated and compared with other BH3 domains Results and discussion FACS results A cell-based system for HBV genome A, B, and ... effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and C and incubated for 24 h...
... transfected with empty vector and cells treated by 50 µM cisplatin for 16 hr were set as (-) and (+) controls Transfected cells were collected at 24 hr and 48 hr after incubation and analyzed by Apoalert™ ... amino acid sequences were analyzed by using Vector NTI9 Result was generated and compared with other BH3 domains Results and discussion FACS results A cell-based system for HBV genome A, B, and ... effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and C and incubated for 24 h...
... column and was detected by the phenol-sulfuric acid assay as a single peak No absorption at 280 and 260 nm in UV absorption spectra of TMP-A demonstrated the absence of protein and nucleic acid ... Cl− ) and eluted stepwise with 0, 0.1, 0.2, 0.3, 0.4, 0.5 and 1.0 M NaCl The eluate was monitored by the phenol-sulfuric acid method [8] The M NaCl eluation was concentrated, lyophilized and purified ... with 90% aqueous formic acid (3 mL) for 10 h at 100 ◦ C in a sealed tube The methylated sugars were derivatized using the method described and analyzed by GC–MS 2.5 UV and infrared (IR) spectra...
... determined and compared with those of the synthesised compounds (Fig 3) The antioxidantactivity of BHA and AA was still lower than that of the compounds (a) and (d) In general, the antioxidantactivity ... 3) Evaluation of In Vitro AntioxidantActivity 90 antioxidantactivity of 5H-dibenz[b,f]azepine and its derivatives on human LDL oxidation and lipid peroxidation activity in the liposome system ... that the Antioxidantactivity (%) Antioxidantactivity (%) M 100 100 5µM 80 80 60 60 40 40 20 20 00 4 22 6 Time (h) Time(h) a a b b c c d d e e f f AA AA BHA BHA 10 µ M 100 100 Antioxidant activity...
... investigation as a potential candidate as an antipsychotic agent with novel and clinically important properties A putative combination of dopaminergic antagonism andantioxidantactivity of a compound ... of 40 and 80 µmol/kg reduces the activity by 39 and 82% respectively (Number of experimental animals: 3–6) ii In apomorphine pretreated rats, TPBIA (80 µmol/kg) reverses the hyperactivity and stereotype ... neuroleptic effects, 3) to test its antioxidantactivity Figure The Ugo-Basile activity cage (type 7401) The Ugo-Basile activity cage (type 7401) Materials and Methods Synthesis of TPBIA Key step...
... were kept under standard conditions, two per cage, with food and water ad libitum and a 12 h/12 h light/dark cycle The mouse anti-rat-CD4 mAbs W3/25, OX35, and RIB5/2 (IgG1, IgG2a, and IgG2a, respectively) ... IFN-, IL-2, IL-4, and IL-10 by sandwich ELISA with the above-mentioned capture and detection antibodies Recombinant cytokine standards were as follows: IFN- (Laboserv), IL-2, IL-10, and IL-4 (Pharmingen); ... the other hand, a contribution of the strikingly higher kdiss of the mAb RIB5/2 to its differential clinical effects cannot be excluded, in analogy to high and low kdiss for agonistic and antagonistic...