lipid polarity and sorting in epithelial cells

... Endothelial cells are continuously subjected to mechanical strain with each heart beat, epithelial cells lining the alveoli in the lungs are stretched during inspiration, and epithelial cells lining ... FAK and Fyn/Shc pathways represent two main signaling pathways activated by integrins Apart from these main signaling pathways, integrins can also initiate several other signaling pathways leading ... characteristic of integrin mediated cell substrate adhesion     ECM  α β Integrins  α‐actinin, talin Ca2+ Na+, K+  Cytoskeleton Ions  PI3 Kinase  FAK, MAPK Lipid kinases  Protein kinases PLC, PLA2 ...

Ngày tải lên: 11/09/2015, 09:04

... H2O2-induced apoptosis in transfected C2C12 cells Furthermore, there was no direct interaction between HSP70 and Smac proteins, and the ATP-binding domain of HSP70, rather than the peptide-binding ... interaction between HSP70 and Smac HSP70 contains three functional regions: the ATPbinding domain, the peptide-binding domain, and the EEVD motif Although the EEVD motif is considered to be involved ... the ATP-binding domain of HSP70 is critical for sequestering AIF in the cytosol [29] In the present study, we demonstrated that the ATP-binding domain of HSP70 was indispensable for inhibition...

Ngày tải lên: 16/03/2014, 01:20

... pathways, the ERK1 ⁄ and c-Jun N-terminal kinase pathways, through AP-1 signaling [26] AP-1 family proteins are good candidates for mediating NGF-induced miR-221 ⁄ 222 expression in PC12 cells A previous ... function in different biological processes Our findings provide new insights into the MAPK signaling pathway 3274 Experimental procedures Cell culture and transfection PC12 cells were maintained in ... effectively induce miR221 and 222 in PC12 cells Finally we identified the BH3-only protein Bim, which is involved in NGF-dependent neuronal survival [17–19], as a potential target of miR-221 and 222...

Ngày tải lên: 16/03/2014, 01:20

... increased during morphogenesis of the embryo Fluorescence was observed as strong punctate staining in intestinal cells and in the epidermis The number and intensity of fluorescent structures increased ... indirect, in lipid metabolism and respiration, it stills remain unclear One hypothesis might be that GST kappa plays a role in the folding of proteins involved in lipid synthesis or respiration Indeed, ... adipose tissue and interact with adiponectin Adiponectin is an adipokine specifically secreted from adipose tissue, which plays a key role in glucose and lipid metabolism in insulin-sensitive tissues...

Ngày tải lên: 16/03/2014, 03:20

... reading frame During the cloning procedure, a cysteine residue was introduced between this signal and the mature poneratoxin Surprisingly, poneratoxin expression was detected only in cells infected ... studied in the laboratory [6,48,49] and in controlled conditions in the field [47,50] We thought that poneratoxin, another insect toxin, might be a good candidate for the reinforcement of the insecticide ... of using it for engineering a baculovirus capable of serving as a bio-insecticide Peptide synthesis and expression of recombinant poneratoxin Using the published poneratoxin sequence [12], the...

Ngày tải lên: 30/03/2014, 13:20

... used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning ... type A and B influenza viruses have genomes consisting of eight negative-sense single-stranded viral RNAs (vRNAs), each of which contains a coding region and terminal 5' and 3' noncoding regions ... read and approved the final manuscript Acknowledgements We thank Hong Jin, Bin Lu, Zhongying Chen and Helen Zhou for providing plasmid clones of influenza segments and Chin-Fen Yang for providing...

Ngày tải lên: 18/06/2014, 18:20

... used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning ... type A and B influenza viruses have genomes consisting of eight negative-sense single-stranded viral RNAs (vRNAs), each of which contains a coding region and terminal 5' and 3' noncoding regions ... read and approved the final manuscript Acknowledgements We thank Hong Jin, Bin Lu, Zhongying Chen and Helen Zhou for providing plasmid clones of influenza segments and Chin-Fen Yang for providing...

Ngày tải lên: 20/06/2014, 01:20

... mediators and cytokines is inhibited in a process in which Fc⑀RI contributes to the ITIM-dependent inhibition of its own intracellular signalling This is achieved by the Fc⑀RI-associated tyrosine kinase ... Activating and inhibitory signaling in mast cells: new opportunities for therapeutic intervention? J Allergy Clin Immunol 2000;106:429–40 Blank U, Ra C, Miller L, et al Complete structure and expression ... activation of protein kinase B by insulin and IGF-1 EMBO J 1996;15:6541–51 Coaggregation of Fc⑀RI with Fc␥RIIB in Mast Cells — Ekoff et al 97 33 Aman MJ, Lamkin TD, Okada H, et al The inositol phosphatase...

Ngày tải lên: 08/08/2014, 21:20

... types, including leukocytes, epithelial cells, endothelial cells and fibroblasts [1316] In endothelial cells and fibroblasts, however, most other cytokine combinations have not been evaluated for induction ... In order to obtain more insight into CXCL10 processing and the role of CXCL10 and CD26/DPP IV in various rheumatic diseases, including psoriatic arthritis (PsA), ankylosing spondylitis (AS) and ... CXCL8 and CXCL10 induction in fibroblasts by IL-1β and interferons CXCL8 and CXCL10 induction in fibroblasts by IL-1β and interferons Confluent fibroblast monolayers were incubated with IL-1β in...

Ngày tải lên: 09/08/2014, 08:22

... is the finding that in head and neck cancer cells, BA-induced cytotoxic effects were linked to a decreased level of Bax, an inducer of apoptosis [33] In prostate cancer cells, the combination ... apoptosis inhibitor survivin (Figure and 4) In agreement with our current findings, BA was also found to inhibit the migration of glioma (C6), lung carcinoma (A549) and medulloblastoma (TE671) cells ... 23 μM in U251MG cells and 24 μM in U343MG cells under normoxic conditions after an incubation time of 24 h (Figure 1) In addition, longer incubation with BA led to increased cytotoxicity in both...

Ngày tải lên: 09/08/2014, 09:21

... Shanghai, China) Cells were infected with Ad-bFGF-siRNA at 100 MOI for h in 6-well plates, incubated in fresh DMEM for 72 h, and collected and resuspended in fresh medium Cells were then incubated ... receptor-associated tyrosine kinases or growth factor receptor intrinsic tyrosine kinases, besides antagonizing the function of relevant kinases or receptors, targeting the over-expressed ligands that inappropriately ... glioma in vitro and in vivo bFGF exerts functions via its specific binding to the high affinity transmembrane tyrosine kinase receptors [23] and the low affinity FGF receptors (FGFR1-4) [24] The binding...

Ngày tải lên: 10/08/2014, 10:21

... aqueous control preparation containing 0.75% peppermint oil as a masking agent was administered in a single-blinded manner as a single 50 μl application in each nostril using a metered spray applicator ... described [44] In brief, cells grown to 70% confluence on 12-well inserts were treated as indicated for 15 and incubated for 12 hr at 37°C Rinsed cells were fully lysed and luciferase luminescence ... occasional mononuclear and infrequent eosinophilic cells This increase in inflammatory response was statistically significant within this treatment group In the same subjects undergoing pretreatment...

Ngày tải lên: 12/08/2014, 13:22

... cellular lipid membrane domains that are enriched cholesterol and sphingolipids, has been described following infection with P aeruginosa, and has been linked to inflammatory signaling and apoptosis ... and SREBP expression in DC from WT and CF mice infected with P aeruginosa Cav1 and SREBP expression in DC from WT and CF mice infected with P aeruginosa DC from WT and CF mice were infected in ... signaling, cell cycling and apoptosis (Supplemental Tables and 2) Especially, inflammation related genes were up-regulated upon the P aeruginosa infection in both WT and CF mice, including 27 interferon-stimulated...

Ngày tải lên: 12/08/2014, 14:20

... (erlotinib, efitinib, and Gifitinib, for example), target to the ATP-binding pockets within the EGFR tyrosine kinase (TK) domain and block the phosphorylation and the following signalling 3) ... methods for studying rafts is interrupting rafts by changing the amount of cholesterol in cells, which includes increasing its amount by adding in extra cholesterol and extracting it by drug treatments ... such as binding with ligands and subsequent activation Interestingly, again, opposing effects were observed in different settings of experiments In artificial 20 membranes, the binding of EGF-EGFR...

Ngày tải lên: 10/09/2015, 09:25

... FEBS 2003 PP17b in apoptosis and differentiation of HeLa cells (Eur J Biochem 270) 1177 role in intracellular lipid metabolism rather than in secretory protein sorting, taking into account that ... detected in the cytosol (Fig 4A) In lipid- loaded cells, amounts of PP17a, PP17b and PP17c were increased in the cytosol fraction, and in parallel an intense elevation of PP17b in the lipid- droplet ... factor binding sites clustered in the vicinity of the Inr might serve in transcription initiation (Fig 1) Analysis of the 1.5-kb 5¢ flanking region, attempting to get further insight into the...

Ngày tải lên: 23/03/2014, 21:20

... including asthma and other pulmonary inflammatory diseases and have shown some efficacy in pre-clinical models after nebulisation, intratracheal injection, intravenous or intraperitoneal administration ... asODN and siRNA in the murine nose in vivo Inefficient transfection of airway epithelial cells may be a key limiting factor in achieving meaningful levels of down-regulation of gene expression in ... further Interestingly, the intracellular distribution of asODN and siRNA following in vitro transfection into epithelial cells was very different, with asODN-lipoplexes accumulating rapidly in the...

Ngày tải lên: 12/08/2014, 16:20

... may occur during the initial h window of infection We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 and h postinfection, and examined the cells under ... to fully understand HSV-1 infection of all target cells, including RPE cells, our study provides new starting points for understanding viral pathogenesis in the retina and advancing novel therapies ... entry into RPE cells V Tiwari et al Fig Role of nectin-1 during HSV-1 entry into RPE cells (A) Antibody against nectin-1 significantly inhibits HSV-1 entry into cultured RPE cells Cells (indicated)...

Ngày tải lên: 18/02/2014, 14:20

... component In this study, the authors imply that P2X7 receptors in nonraft microdomains of submandibular gland cells are solely involved in ion channel activity, and that receptors present in lipid ... immunofluorescence of Caveolin-1 and P2X7 in alveolar epithelial E10 cells To examine the possible colocalization of the purinergic P2X7 receptor with Caveolin-1 in alveolar epithelial E10 cells, we analyzed ... localization in subconfluent cells was determined Figure 5A shows a Caveolin-1 staining pattern at the cell surface, confirming the known caveolar location of Caveolin-1 In addition, intracellularly distinguishable...

Ngày tải lên: 19/02/2014, 00:20

... these compounds against cardiovascular disease [11] Dietary lipids, including fats, cholesterol and lipid- soluble antioxidants, are absorbed in the intestine and secreted into lymph in chylomicrons, ... prepared by sonication of a lipid mixture containing 70% trilinolein, 2% cholesterol, 5% cholesteryl ester and 25% phospholipids, in Tricine buffer (20 mM, pH 7.4) containing 0.9% NaCl, followed ... remnants are able to induce foam cell formation in human monocyte-derived macrophages (HMDM) [21] and in human and murine macrophage cell lines [22–24], and that, in striking contrast to LDL,...

Ngày tải lên: 16/03/2014, 16:20

... (Sigma) containing 10% (v/v) fetal bovine serum and 100 unitsÆmL)1 penicillin The cells were maintained in humidified atmosphere with 5% (v/v) CO2 at 37 °C The eRBCC cDNA was introduced into the pcDNA3 ... Conserved cysteine/ histidine residues in the RING finger domain and B box domain are circled The potential coiled-coil and B30.2 domain are underlined Potential polyadenylation site in the 3¢-untranslated ... present in the nucleus of the epithelial cells of the secondary lamella including the chloride cells and pavement cells whose nuclei are labeled by arrows and double arrowheads, respectively, in Fig...

Ngày tải lên: 17/03/2014, 10:20