lipid packing and motion in rabbit small intestinal brush border membrane

... Haematoxylin–eosin staining Haematoxylin–eosin staining was carried out following the routine histological method described by Kayser and Bubenzer [38] The haematoxylin–eosin staining technique ... the small intestine was cut and flushed two to three times with 0.9% saline One end of the intestine was tied with a thread and filled with rinsing buffer containing mm d,l-dithiothreitol in normal ... preparation of efficiently transporting vesicles from small intestinal brush border membranes Their use in investigating some properties of d-glucose and choline transport Biochim Biophys Acta...

Ngày tải lên: 18/02/2014, 16:20

Ngày tải lên: 29/10/2014, 22:15

Ngày tải lên: 22/02/2014, 08:20

... observed as strong punctate staining in intestinal cells and in the epidermis The number and intensity of fluorescent structures increased strongly in the intestine during larval development (Fig ... indirect, in lipid metabolism and respiration, it stills remain unclear One hypothesis might be that GST kappa plays a role in the folding of proteins involved in lipid synthesis or respiration Indeed, ... adipose tissue and interact with adiponectin Adiponectin is an adipokine specifically secreted from adipose tissue, which plays a key role in glucose and lipid metabolism in insulin-sensitive tissues...

Ngày tải lên: 16/03/2014, 03:20

... Dubach and Li [11], and Leightner [30, 31], in Thailand, Indonesia, and South Korea during the East Asian cri- sis in 1990s, bubbles occurred in the price of housing, office space, and land, which ... Diamond and Dybvig [8] and Chang and Velasco [7] 4 world of inefficient and efficient investment coexistence Then, bubbles absorb savings from the inefficient and help increase the efficient investment ... constraint The credit constraint has no major part in sustaining bubbles as the reason bubbles can be sustained is purely due to the inefficiency in the world’s investment However, the credit constraint...

Ngày tải lên: 09/09/2015, 18:50

... [123] and the serine-threonine kinase RIP (Receptor interacting protein) instead of FADD, and initiate the activation of NF-$B (Nuclear Factor $B) and JNK (c-Jun N-terminal Kinase) signaling pathways ... CED-3 The mammalian family contains many members, most participating in apoptosis, whilst the remaining family members are involved in cytokine processing and inflammation [25-27] (Figure 2) ... prior to the binding of their ligand This is made possible by the presence of a particular domain in their N-terminal part, Pre-Ligand Assembly Domain (PLAD) This implies that ligand binding does...

Ngày tải lên: 11/09/2015, 10:14

... 34B) Interestingly, down-regulation of survivin protein levels upon treatment with LY30 was inhibited in the presence of the proteasome inhibitor lactacystin Inversely, inhibition of protein synthesis ... in a marked increase in cell death as reported in several studies Inhibition of the proteasome lead to an expected increase in survivin protein level in untreated cells and cells treated with ... resulted in an increase in cell death for all treatment combination Taken together, these results indicate that LY30 is able to induce Hsp27 phosphorylation by activating p38 MAKP as well as inhibiting...

Ngày tải lên: 11/09/2015, 10:15

... projectile velocity is: v y = v0 sin θ − gt At point A: v y = → v0 sin θ − gt → t = H = y (t ) = ( v0 sin θ ) t − vo sin θ o H= 2g v0 sin θ g gt v sin θ g  v0 sin θ  = ( v0 sin θ ) −  ÷ → g 2 g  (4 ... correspond to point O and is of no interest gt Solution v0 sin θ − = This solution correspond to point A 2v0 sin θ From solution we get: t = If we substitute t in eqs.2 we get: g 2 2vo vo R= sin θ o cos ... y = yo + ( v0 sin θ ) t − (eqs.4) If we eliminate t between equations and we get: v y − ( v0 sin θ ) = −2 g ( y − yo ) g Here xo and yo are the coordinates of the launching point For many problems...

Ngày tải lên: 13/12/2013, 05:15

... parameters which satisfies Eq (5) and has infinite magnitude Finding all real solutions of Eq (5) – both finite and at infinity – has been thus reduced to determining all real finite solutions of Eq (6), ... C Pinto, V Petuya, A Hernandez Motion pattern singularity in lower mobility parallel manipulators 489 Author Index 497 Methods in Kinematics J Andrade-Cetto, F Thomas Wire-based tracking using ... main difficulties however, is in appropriately choosing base rotation commands so as to prevent wire crossings Considering that wire end-point displacements are sufficiently small per sampling interval,...

Ngày tải lên: 13/02/2014, 09:20

... cavities, one in the hydrophobic core (M88V) and the other in the protein–RNA interaction (T45S), decrease the stability of the capsid Our findings illuminate the role of packing in the icosahedral ... sterically interfere with side-chain packing in the protein’s interior Mutations that create cavities in hydrophobic environments generally cause proteins to become less stable [44,45] Using the ... residue with valine (C and D), the cavity volume increased to 43 A3, reflecting a reduction in the surface area of the residue (% 59 A2) and in the interactions occurring there (corresponding to the...

Ngày tải lên: 19/02/2014, 12:20

... parameters which satisfies Eq (5) and has infinite magnitude Finding all real solutions of Eq (5) – both finite and at infinity – has been thus reduced to determining all real finite solutions of Eq (6), ... C Pinto, V Petuya, A Hernandez Motion pattern singularity in lower mobility parallel manipulators 489 Author Index 497 Methods in Kinematics J Andrade-Cetto, F Thomas Wire-based tracking using ... main difficulties however, is in appropriately choosing base rotation commands so as to prevent wire crossings Considering that wire end-point displacements are sufficiently small per sampling interval,...

Ngày tải lên: 05/03/2014, 17:20

... separately from emotions and sensations These domains, far from being independent, are intimately intertwined in many ways and by means of mechanisms we are just beginning to investigate We start ... empathetic feelings in the observer and the representational content of the works in terms of the actions, intentions, objects, emotions and sensations depicted in a given painting or sculpture; and (ii) ... (reviewed in Refs [15,17]) Recent studies in macaques [18] and humans [19] demonstrated that mirror neurons not only underpin action understanding, but they are also involved in understanding the intentions...

Ngày tải lên: 07/03/2014, 17:20

... Advertising, Publishing, Printing and Packaging (SETA) MERSETA Manufacturing, Engineering and Related Services Education and Training Authority MQA Mining Qualifications Authority (SETA) MSE Micro and ... Accounting Services Sector Education and Training Authority FET Further Education and Training FIETA Forest Industries Education and Training Authority FOODBEV Food and Beverages Manufacturing Industry ... Trade and Industry ESETA Energy Sector Education and Training Authority ETDP SETA Education and Training and Development Practices Sector Education and Training Authority FASSET Financial and Accounting...

Ngày tải lên: 15/03/2014, 04:20

... VIRAL GENOMES • Depending on the virus, viral genomes: • May be double-stranded DNA, singlestranded DNA, double-stranded RNA or single-stranded RNA • Are organized as single nucleic acid molecules ... fibers, plate and pin TAXONOMY OF VIRUSES • Viruses are not organisms and are not classified in the kingdoms of life • Regarded as self-replicating portions of the genomes of organisms • In comparison ... which may include several species(e.g swine flu and rabies) • Some viruses have host ranges so narrow that they can: • infect only one species(e.g phages of E.coli • Infect only a single tissue...

Ngày tải lên: 15/03/2014, 13:06

... production and storage of excess membrane material is possible by including a pool of membrane lipids in our model Ergosterol Phosphatidate Phosphatidylcholine Phosphatidylethanolamine Phosphatidylinositol ... pathways involved in the production of membrane lipids and MP, and their interaction with TAG mobilization, are illustrated in Fig By Fig Schematic representation of FA, neutral, and phospholipid ... acid (40 : 15 : 0.5), and stained at 120 °C for 15 after submerging the plate in a solution containing 3.2% H2SO4 and 0.5% MnCl2 Lipids were quantified against appropriate standards by 5560 ð1Þ...

Ngày tải lên: 16/03/2014, 04:20

... in Fig 1A indicate that Pgp is localized in membrane microdomains that are distinct from classical lipid rafts containing Src-family tyrosine kinases The membrane microdomains containing Pgp displayed ... proteins were, once again, very distinct (Fig 4G,H) Both caveolin-1 and Pgp maintained the pattern described above Pgp displayed staining at the plasma membrane but also 4929 P-glycoprotein in intermediate-density ... for the maintenance of some types of membrane microdomains, for example those containing K-ras [45] and galectin-4 [46] In contrast, the Pgp-containing Triton X-100 microdomains remaining after...

Ngày tải lên: 16/03/2014, 23:20

... trypsinolysis was determined by plotting ln(At ⁄ Ao) (where Ao and At denote the intensity of protein bands of intact unhydrolized protein at the beginning and at the fixed time of incubation) against ... accession code 1GME) The N- and C-terminal domains are indicated by N and C correspondingly All b-strands are numbered and the b5 and b7 strands are shown in red and blue, respectively G104 (equivalent ... flatworm Taenia saginata Tsp36, containing two a-crystallin domains in the single polypeptide chain [12] Ten different sHsp are encoded in the human genome and are differently expressed in human tissues...

Ngày tải lên: 23/03/2014, 07:20

... expressed in the apical membranes of intestinal (PEPT1) and renal (PEPT1 or PEPT2) epithelial cells Absorption of intact di- and tri-peptides and related mimetics at the intestinal epithelium or their ... (1982) Activity of alafosfalin against bacterial pathogens of the gastrointestinal tract in vitro and in vivo Current chemotherapy and immunotherapy: Proceedings 12th Int Congr Chemother 1, 340–342 ... cells, 20 and 50 lM for SKPT cells) in the presence of increasing amounts of alafosfalin The linear, nonmediated transport components, i.e simple diffusion plus tracer binding, of 19.2% and 5.1%...

Ngày tải lên: 23/03/2014, 12:20

... to lipid droplets and milk lipid globule membranes, showing that PP17b binds to heterologous intracellular lipid droplet surfaces indicating its function in lipid deposition and/ or mobilization ... factor binding sites clustered in the vicinity of the Inr might serve in transcription initiation (Fig 1) Analysis of the 1.5-kb 5¢ flanking region, attempting to get further insight into the ... detected in the cytosol (Fig 4A) In lipid- loaded cells, amounts of PP17a, PP17b and PP17c were increased in the cytosol fraction, and in parallel an intense elevation of PP17b in the lipid- droplet...

Ngày tải lên: 23/03/2014, 21:20

... is also determined by a large number of lipids and proteins other than caveolin For example, glycosphingolipids are enriched in lipid rafts and are capable of inducing and modulating signal transduction ... GPI-anchored ephrin-A5 in lipid rafts The ephrin-A5 induced signalling results in increased tyrosine phosphorylation of several raft proteins and recruitment of the Src family kinase Fyn to lipid rafts ... to rafts following cross-linking [122] Similarly, upon cross-linking the BCR translocates rapidly into a lipid raft containing the Src family kinase Lyn, which is involved in the initial phosphorylation...

Ngày tải lên: 31/03/2014, 21:21