lipid modulation of cell functions

... optimum T -cell responsiveness after vaccination These effects might also rely on increased amounts of SPPV induced enhancement of IL-12 due to ability of IL12 to induce early phase of NK and T cell ... accessory cells and lymphocytes, but it is also a key molecule for programming the macrophage and dendritic cell functions [4] One of the major effects of IL-12 on macrophages and dendritic cells ... accompanied by a persistent state of oxidative siege, and the survival of a given cell is determined by its balance of reactive oxygen intermediates and antioxidants Disturbance of this balance can lead...

Ngày tải lên: 18/06/2014, 22:20

... optimum T -cell responsiveness after vaccination These effects might also rely on increased amounts of SPPV induced enhancement of IL-12 due to ability of IL12 to induce early phase of NK and T cell ... accessory cells and lymphocytes, but it is also a key molecule for programming the macrophage and dendritic cell functions [4] One of the major effects of IL-12 on macrophages and dendritic cells ... accompanied by a persistent state of oxidative siege, and the survival of a given cell is determined by its balance of reactive oxygen intermediates and antioxidants Disturbance of this balance can lead...

Ngày tải lên: 20/06/2014, 04:20

... secondary modulation of signalling pathways downstream of Fas may alter significantly the susceptibility of cells to Fas induced cell death [17] However, the expression of Fas receptor on the cell ... higher rate of spontaneous apoptosis but also were significantly more susceptible to FasL mediated cell death Of note, apoptosis of fibroblast-like cells is regulated at a number of different ... TNF-α is involved in a variety of inflammatory and tissue repair processes, the question of how it affects the apoptotic response of potential target cells is of major interest R254 In the present...

Ngày tải lên: 09/08/2014, 01:23

... purines on cell growth 38 Table Strategies of the extracellular mechanism studies of AIA in BHK 184 cells Table Strategies of the extracellular mechanism studies of AIA in HeLa 185 cells xv ABBREVIATIONS ... Effect of NBTI on AIA in SKW6.4 cells 123 Fig 3.40 Effect of dipyridamole on AIA in SKW6.4 cells 125 Fig 3.41 Effect of NBTI on AIA in H9 cells 127 Fig 3.42 Effect of dipyridamole on AIA in H9 cells ... effects of adenosine on HeLa cells 66 Fig 3.4 Apoptotic effects of adenosine on SKW6.4 and H9 cells 67 Fig 3.5 Apoptotic effects of adenosine on SY5Y and MN9D cells 69 Fig 3.6 Apoptotic effects of...

Ngày tải lên: 11/09/2015, 16:07

... release is the modification of the cysteine ligands of zinc Aldehydes increase the concentration of available cellular zinc Cultured human hepatocellular carcinoma (HepG2) cells were used to examine ... for measurement of intracellular zinc HepG2 cells without any treatment have a fluorescence signal that corresponds to 15.4% saturation of Zinquin with zinc (Fig 5A) Treatment of cells with acrolein ... expression of MT The MT concentration in control HepG2 cells is 75.4 ± 7.6 ngÆ(g cells))1 (Fig 6) Treating the cells with ethanol, a known inducer of MT [17], for 12 h increases the concentration of...

Ngày tải lên: 19/02/2014, 06:20

... media of culture passages five to seven of primary neural stem cells from cortices of E16 Sprague-Dawley rat embryos in three separate experiments A preponderance of NGF in pg per day of culture of ... foot faults (DOFFF) is the difference between the number of faults for the right and left forelimbs SOFFF and DOFFF were used as an index of motor deficit CSF aspiration 80 μL of CSF were aspirated ... number of foot faults in the left and right forelimbs The sum of foot faults (SOFFF) in the forelimbs is defined as the number of faults of right and left forelimbs, whereas the differentiation of...

Ngày tải lên: 18/06/2014, 16:20

... concentration of viral pro- Figure of hypertonic medium Kinetics intracellular RSV proteins in cells incubated in Kinetics of intracellular RSV proteins in cells incubated in hypertonic medium HEp-2 cells ... and re-appearance of intracellular viral pro- Figure Confocal laser scanning image of intracellular RSV proteins Confocal laser scanning image of intracellular RSV proteins HEp-2 cells had been ... viral glycConfocal laser scanning image of the distribution of viral glycoproteins on cell surface of epithelial cells HEp-2 cells had been infected at m.o.i of 50 for 12 h then incubated with anti-RSV...

Ngày tải lên: 18/06/2014, 18:20

... Mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells J Virol 1979, 29:677-697 Foster TP, Kousoulas KG: Genetic analysis of the role of ... membrane topology of syncytial domains of herpes simplex virus type glycoprotein K (gK): the UL20 protein enables cell surface localization of gK but not gK-mediated cell- to -cell fusion J Virol ... intracellular transport, cell- surface expression and TGN localization However, mutagenesis of both Y38 and Y49 amino acid residues in the amino terminus of UL20p, inhibited virus-induced cell...

Ngày tải lên: 18/06/2014, 18:20

... concentration of viral pro- Figure of hypertonic medium Kinetics intracellular RSV proteins in cells incubated in Kinetics of intracellular RSV proteins in cells incubated in hypertonic medium HEp-2 cells ... and re-appearance of intracellular viral pro- Figure Confocal laser scanning image of intracellular RSV proteins Confocal laser scanning image of intracellular RSV proteins HEp-2 cells had been ... viral glycConfocal laser scanning image of the distribution of viral glycoproteins on cell surface of epithelial cells HEp-2 cells had been infected at m.o.i of 50 for 12 h then incubated with anti-RSV...

Ngày tải lên: 20/06/2014, 01:20

... OVA-specific CD8+ T cells was determined by flow cytometry (FACScan, BD Biosciences) and expressed as a percentage of the total number of OVA-specific CD8+ cells Analysis of the Dendritic Cell Population ... with cell recoveries in the range of to million per gram of lung tissue The CD11c+ cells were then seeded into 12-well plates at 105 cells per well and cultured for 48 hours in the presence of ... cytometry (FACScan, BD Biosciences) The numbers of CD11c+b+ cells were expressed as a percentage of the total CD11c+ cells Analysis of the Dendritic Cell Population in BAL Fluid BALB/c mice were...

Ngày tải lên: 08/08/2014, 21:20

... example of 3-D reconstruction of nuclei, of chromatin distribution and of sites of lipid peroxidation in cells undergoing oxidative stress by nanoparticles Chromatin reorganization in cell nuclei ... considered to correspond to a cluster of quantum dots Figure 3-D model of the nucleus of the same MCF-7 cell as in Fig 3-D model of the nucleus of the same MCF-7 cell as in Fig The translucent surface ... number not for citation purposes) Journal of Nanobiotechnology 2006, 4:10 Figure 3-D model of the nucleus of a PC12 cell 3-D model of the nucleus of a PC12 cell The translucent surface and small...

Ngày tải lên: 11/08/2014, 00:22

... variety of transport and signal transduction processes in cells infected by HIV1 Modulation of cellular transport paths by Nef affects the surface presentation of an increasing number of cell receptors ... changes the incorporation of individual lipid classes into lipid microdomains of the host cell plasma membrane To this end, we performed a lipid analysis of different frac- Page of 12 (page number not ... remain unclear In light of the effects of Nef on lipid transport discussed above, the lack of differences in the lipid composition in total cell lysates suggest effects of Nef on lipid sorting and/or...

Ngày tải lên: 13/08/2014, 05:22

... expression of cell surface receptors was performed in whole blood, drawn in EDTA tubes A total of 100 μl of whole blood from patients and controls were stained with μl of CD14-PerCP and μl of CD66-FITC ... stained with the following isotypes: 10 μl of mIgG2a-PE and μl of mIgG2b-APC (tube 1); 10 μl of TLR2-PE and μl of CD11c-APC (tube 2) or 20 μl of TLR4-PE and μl of CD11bAPC (tube 3) Samples were incubated ... dynamic of sepsis is pointed out by the inclusion of patients at different stages of the disease; sepsis, severe sepsis and septic shock Data are provided showing modulation of neutrophils functions...

Ngày tải lên: 13/08/2014, 10:20

... airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice Genetic Vaccines and Therapy 2010, 8:2 Page of ... The degree of cellular infiltration was scored as described previously [6] Inflammatory changes were graded by histopatholog- Page of ical assessment using a semiquantitative scale of 0-5 for ... was aspirated and pooled into two of the tubes with PBS for a total volume of 75 ml The 15 ml of Ficoll Paque was placed into three 50 ml tubes/ group 25 ml of the diluted filter material was...

Ngày tải lên: 14/08/2014, 19:22

... role of lipid rafts and Cav-1 in early stage of autophagy 130 Figure 6.2 Model of the regulatory role of lipid rafts and Cav-1 in late stage of autophagy 134 Figure 6.3 Model of ... in lipid rafts studies (Chamberlain, 2004) Figure 1.8 A simplified model of lipid rafts in cell membrane (Dart, 2010) 1.2.1.2 Classification of lipid rafts 29 There are mainly two types of lipid ... degradation of lipid droplets by autophagy is termed as lipophagy Lipid droplets are monolayer cytosolic organelles for intracellular deposits of lipid esters (Singh and Cuervo, 2012) The role of autophagy...

Ngày tải lên: 09/09/2015, 08:13

... pyramidal cell layer The principal cell of the hilus is the mossy cells The cell bodies of the mossy cells are large (25-35 µm), triangular or multipolar in shape The dendrites of mossy cells are ... axon from the basket cell extends transversely from the cell body and forms a basket plexus innervating the cell body of pyramidal cells The second type of interneuron with cell body in stratum ... of the principal (pyramidal) cells The pyramidal cells of hippocampus along with the principal (granule) cells of the dentate gyrus make up the bulk (~90%) of the cell population in the two regions...

Ngày tải lên: 16/09/2015, 17:14

... the balance of expression of members of the Myc/Max/Mad network of proteins (see Fig 1) may dictate the response to loss of Myc (Walker et al 2005) In mice, the effects of deletion of the c-myc ... excision of c-myc (de Alboran et al 2001) Importantly, deletion of the mnt gene, which encodes a member of the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting ... neuronal precursor cells leads to a dramatic loss of proliferative capacity of such cells (Knoepfler et al 2002) As long as questions of redundancy between different members of the myc gene family...

Ngày tải lên: 25/10/2013, 21:20

... in Mouse Models of Cell Cycle Regulators One of the hallmarks of cancer cells is misregulation of cell proliferation Cancer cells are able to evade the normal signals that stop cell division and ... the mechanisms of regulation of normal cell proliferation sets the ground for understanding the deregulated proliferation characteristic of a cancer cell regardless of the type of cancer or where/how ... functions are cell cycle phase-specific Mouse Models of Cell Cycle Regulators The advantages of mouse models over in vitro studies is that it highlights the functions of a particular cell cycle regulator...

Ngày tải lên: 25/10/2013, 21:20

... have focused upon the role of miRNAs in regulatory network of various cell signaling pathways We summarize the current knowledge of the interdependence of miRNA and cell signaling pathways, which ... involved in various cellular functions, including cell proliferation, differentiation and migration [13] Recently, the mechanisms of transcription and the functional roles of miRNAs associated ... stimulation of miRNAs and cell signaling HER2 ⁄ neu signaling and that miR-21 suppresses the metastasis suppressor protein PDCD4 (programmed cell death 4) in breast cancer cells The expression of miR-21...

Ngày tải lên: 14/02/2014, 19:20