lineage tracing of tissue specific stem cells in vivo
Báo cáo y học: " In situ detection of Gag-specific CD8+ cells in the GI tract of SIV infected Rhesus macaques" pptx
Ngày tải lên :
12/08/2014, 23:23
... verifying that nonspecific binding of the Qdot 655 multimer is low Staining pattern and staining intensity of Gag CM9 Qdot 655 multimer positive cells Confocal microscopy revealed a punctate staining ... ranging from 2.43%- 9.59% of all CD8+ cells, confirming reports using flow cytometry or in situ staining of fresh tissues using tetramers [10,24-28] We also looked for CD8+ T cells recognizing ... http://www.retrovirology.com/content/7/1/12 Page of 14 Figure In situ staining of CD8+Gag CM9+ cells in frozen tissue A) Fluorescence images of a spleen tissue section from an SIV infected Mamu-A*01 positive RM stained with CD8...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
Ngày tải lên :
11/09/2015, 09:16
... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies Evidence that the synergy sequence of fibronectin is ... Owen M, Peck W 1985 ;Lineage of osteogenic cells and their relationship to the stromal system In ed Bone and MineralAmsterdam Elsevier pp :1-25 122 Owen M 1988;Marrow stromal stem cells J Cell Sci...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
Ngày tải lên :
10/08/2014, 23:21
... brought into an operating room and placed in a supine position She was then sedated with propofol mg intravenously (push) and a 20 mg/hour rate of continuous infusion After cleaning her abdominal ... her hip pain improved About a month prior to presentation, she again started having hip pain radiating to the anterior region of the right knee The pain was worse when standing up, walking, and ... CaCl2) In order to inject the mixture of stem cells and PRP, our patient was first placed in a lateral position with her left side down After cleaning with povodine-iodine and draping with sterile...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
Ngày tải lên :
18/06/2014, 16:20
... These data suggest Stem- Kine supplementation increases circulation of cells capable of giving rise to hematopoieticlineage cells in vitro Stem- Kine augments circulation of cells with EPC phenotype ... were required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic...
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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot
Ngày tải lên :
07/08/2014, 23:22
... ytisrevinU lanoitaN luoeS fo ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu ... erew sgod ehT ICS eht retfa keew a demrofrep saw sCSM-BCU eninac fo noitatnalpsnarT :FSCGhmr fo noitcejni dna sCSM-BCU eninac fo noitatnalpsnarT noitcejni eht rof noitulos enilas fo lµ 051 ni 601 ... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
Ngày tải lên :
07/08/2014, 23:22
... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural cells ... cells For group V, the injured site was exposed by dorsal laminectomy and 150 μL of PBS was injected into the spinal cord at locations to depths of mm using a 30 gauge needle (middle of the injury ... Chemicon International, USA) for immunofluorescent determinations of the phenotypes of GFP (+) cells Tissues were incubated in goat serum for h at room temperature The tissues were then incubated...
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Báo cáo y học: "Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis" docx
Ngày tải lên :
09/08/2014, 01:24
... identification of antigen -specific T cells independently of their cytokine secretion profile, the possibility of sorting unstimulated T cells and of having a tool for the antigen -specific detection of T cells ... staining (Fig 2a) In parallel we performed intracellular cytokine staining after peptide -specific stimulation of peripheral MNCs and of the tetramer-sorted T cell line after weeks of culture In ... revealed no specific staining of CD8+ T cells with 0–0.01% tetramer binding to CD8+ T cells Expansion of Chlamydia -specific CD8+ T cells after stimulation with Chlamydia-infected dendritic cells Because...
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Báo cáo y học: "Use of soluble MHC class II/peptide multimers to detect antigen-specific T cells in human disease." pps
Ngày tải lên :
09/08/2014, 03:24
... a narrowing of the TCR repertoire after secondary immunization, resulting from the loss of cells with lowest affinity and an increase in cells with higher affinity for peptide/MHC binding Other ... of the affinity of the TCR for the MHC/peptide Binding of the multimer was shown to be mostly independent of CD4 [6] 262 MHC class II/peptide multimers stained antigen -specific T cells in mice ... hemagglutinin peptide -specific CD4+ T cells in two individuals In both cases, antigen -specific T cells could only be detected following days of in vitro culture with peptide The number of divisions that...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
Ngày tải lên :
09/08/2014, 14:22
... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... or remained uninfected For comparison, an additional set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or ... of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, fixed overnight in PBS-buffered 4%...
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Báo cáo y học: "Nucleosome rotational setting is associated with transcriptional regulation in promoters of tissue-specific human genes" pot
Ngày tải lên :
09/08/2014, 20:22
... frequencies of dinucleotides containing only adenine and/or thymidine (WW dinucleotides), with antiphased periodic frequencies of dinucleotides containing cytidines and/or guanines (SS dinucleotides) ... probably a determinant of the energetically more favorable smooth versus kinked bending of the DNA [10]; and an arginine side-chain is located in the minor groove of all histone-DNA binding sites except ... quantification of tissue specificity is described in Supporting information in Additional file The distribution of the tissue specificity scores for the 4,372 genes was divided into groups containing 1,199,...
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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps
Ngày tải lên :
10/08/2014, 05:21
... the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells in regions of primary PIN lesions (Figure 3B; arrow in (b) indicated P63 ... (arrowhead-indicated in (a)) and in PIN and CaP lesions of the pCXshh-IG-injected prostates (arrowhead-indicated in (b) and (c)) All luminal cells (arrowhead-indicated in (d)) and some basal cells (arrow (1)indicated ... bars: 50 μm in upper (d) of panel A; 10 μm in lower (d) of panel A and in (d) of panel C CaP: prostate cancer; HE: hematoxylin-eosin stain; PIN: prostatic intraepithelial neoplasia; HGPIN: high...
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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx
Ngày tải lên :
10/08/2014, 09:22
... for errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids as ... proteomic detection of AL1A1, AK1C1 and AK1C3 in clinical samples of lung cancers, especially in LCNEC Out of the top five LCNEC -specific proteins, brain-type FABP7 is present in highly infiltrative ... Poly(rC)-binding protein 4.31 3.79E-02 1.01 23 16 Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 Page of 13 Table Significant changes in...
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báo cáo khoa học: " MicroRNAs involved in neoplastic transformation of liver cancer stem cells" pot
Ngày tải lên :
10/08/2014, 10:20
... differentiation of normal stem cells strongly suggest that miRNA may be involved in the transformation of normal stem cells into cancer stem cells Therefore, screening for differences in miRNA expression ... differentiation According to the hierarchical model of cancer, the origin of the cancer stem cells may be long-lived somatic stem cells Therefore, markers of “normal” stem cells are being sought with ... Validation of the differentially expressed miRNAs by qRT-PCR Using a stringent cut-off of P < 0.05, we found significantly altered expression of only of all rat miRNAs analyzed in SP of HCC cells In...
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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc
Ngày tải lên :
10/08/2014, 10:21
... of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs use in ... series of complex interactions exist between BMDC and injured tissues, including the liver Microvesicles are mediators of cell reprogramming Following injury, tissues release chemokines attracting ... tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched...
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báo cáo khoa học: " Identification of tissue-specific, abiotic stressresponsive gene expression patterns in wine grape (Vitis vinifera L.) based on curation and mining of large-scale EST data sets" docx
Ngày tải lên :
11/08/2014, 11:20
... found in seed or other phytoalexin-rich tissues [40,41] Despite the importance of roots, the study of V vinifera root tissues has been rather limited in contrast to the study of berry tissues In ... massexcised in vivo and the resulting plasmids (pBluescript II) were propagated in the E coli SOLR host strain Individual cDNA clones containing inserts were amplified using the TempliPhi DNA Sequencing ... between single pairs of 5’ and 3’ 96-well plates of ESTs, through incorrectly identified pairs of plates of increasing scope The number of times each error occurred (pairs or larger groups of 96-well...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
Ngày tải lên :
12/08/2014, 11:23
... Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of accessory cells, were ... upregulate expression of PD-L1, iNOS, and COX-2 in MSCs, making these molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation ... addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to the co-cultures The addition of these inhibitors resulted in the abrogation of the inhibition of Tcell...
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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf
Ngày tải lên :
12/08/2014, 14:22
... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit IL-1β induced activation of degradative pathways in MSCs It must be noted that in this study ... β1-integrin receptor signalling pathway Several studies have suggested that curcumin interacts with the TGF-β signaling cascade, modulating the action of TGF-β For example in renal cells curcumin...
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Báo cáo y học: "Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase-related genes in nucleus pulposus cells in rabbits" potx
Ngày tải lên :
12/08/2014, 15:22
... remaining nucleus pulposus cells by inhibiting their expressions of degradative enzymes and inflammatory cytokines, resulting in maintaining the structure of the intervertebral disc Conclusions Intradiscal ... pulposus cells Also, synovial MSCs affected the remaining nucleus pulposus cells by inhibiting expressions of degradative enzymes and inflammatory cytokines, resulting in maintaining the structure of ... http://arthritis-research.com/content/12/6/R206 expression of chondroitin sulfate proteoglycan in nucleus pulposus cells induced the holding of water, resulting in improvements of MR imaging in an in vivo study A number of studies have...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
Ngày tải lên :
12/08/2014, 15:22
... self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition, ... differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in the suppression of...
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