line protein expression and morphological analyses

... hsa-mir-29a and b inhibited Nef expression and HIV-1 replication hsa-mir-29a and b inhibited Nef expression and HIV-1 replication (A) Nef expression was inhibited by hsa-miR-29a and 29b HEK293T ... hsa-mir-29a and 29b pEGFP-miRNA construct expressing hsa-mir-29a and 29b (A) Diagrammatic representation of pEGFP-N3 vector containing pre-miR29a and 29b (B) Expression of hsa-mir-29a and 29b confirmed ... hsa-miR-29a and b, along with pCDNA-HA-Nef into HEK293T cells and analyzed Nef expression by immunoblotting As shown in Fig 4A, both the hsa-miR-29a and 29b expressing clones reduced the expression...

Ngày tải lên: 13/08/2014, 05:21

... specific for human Ras-GRF1 Primer pairs: lane 2, ON359 and ON360; lane 3, ON357 and ON358; and lane 4, ON361 and ON360 The PCR products and a DNA size marker, lane 1, were separated on agarose ... that the treatment of NIH3T3 and COS-7 cells with carbachol [9] and lysophosphatidic acid [11], activating both Gq- and Gi-coupled receptors, induces the activation and phosphorylation of Ras-GRF1 ... brain tissue [15– 17], but expression of Ras-GRF1 mRNA has also been reported in some other tissues and non-neural cell lines [18] Several murine Ras-GRF1 cDNAs, encoding proteins of different molecular...

Ngày tải lên: 19/02/2014, 18:20

... Cl, pH 7.5, mM EDTA) The filled and open circles denote native and linear tx3a, respectively (B) Refolding carried out in buffer B (buffer A plus mM GSH and mM GSSG) and in buffer C (buffer B plus ... using various substrates and compared with those of hPDI The recombinant cPDI and hPDI shared similar reductase, oxidase and isomerase activities PDI exhibits both chaperone and antichaperone activities ... and t is the refolding time For the rate of decrease of the linear form, the original data were fitted by Y(t) ¼ [Ymax + (1 ) Ymax)e–kt] · 100%, where Y is the percentage of the linear form, and...

Ngày tải lên: 07/03/2014, 05:20

... First, mammalian expression vectors for FLAG epitope-tagged CCN4 ⁄ WISP1, WISP1v and WISP1vx proteins were constructed, and the proteins were overexpressed by DNA transfection of these expression vectors ... Production of the WISP1v proteins in HCS-2 ⁄ cells and their overexpression (A) Molecular constructs for the overexpression of WISP1, WISP1v and WISP1vx A mammalian expression vector, pFLAG-CMV ... chondrosarcoma and it is one of the cell lines known to retain the characters of chondrocytes, as represented by the expression of type II and type X collagen genes To assess whether WISP1v and WISP1vx...

Ngày tải lên: 07/03/2014, 10:20

... [20,22] and Bacillus subtilis lipase A [23] Results and Discussion Cloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of  31.7 kDa and ... 100 lgÆmL)1 ampicillin and 34 lgÆmL)1 chloramphenicol until a D600 value between 0.6 and 0.8 was reached Protein expression was induced by adding mM IPTG for h at 37 °C and then 16 h at 15 °C ... composed of parallel b strands associated to an antiparallel strand (b2) and is surrounded by helices (a1, a2, a3, a7 and a8) The second domain consists of helices a4, a5 and a6 all clustered on...

Ngày tải lên: 07/03/2014, 16:20

... Kuhl, D (1999) The polo-like protein kinases Fnk and Snk associate with a Ca(2+)- and integrin-binding protein and are Ó FEBS 2002 Interactions between proteins NBR1, CIB and FEZ1 (Eur J Biochem 269) ... later (a) HA-NBR1 (b) FEZ1-EGFP (c) CIB-myc protein expression, (d±f) coexpression of HA-NBR1 and FEZ1EGFP, and (g±i) coexpression of HA-NBR1 and CIB-myc The pattern of CIB staining observed in ... (phosphatase 2B) and also shares 56% protein sequence similarity to calmodulin The protein contains two EF-hand motifs that bind Ca2+ and thus it has been suggested that the protein may act as...

Ngày tải lên: 08/03/2014, 10:20

... [4] and estrogen down-regulate, and thyroid hormone up-regulates SRC-1 cofactor expression in rat tissues [11] The regulation of GR activity by growth factors and cAMP is well established, and ... transcriptional activity [5–7], and a reduction in cofactor TIF2 expression prevents ERa-induced and ligand-induced activation of the promoter 3xERE-TATA [9] Differential expression of cofactors has ... alpha-fetoprotein interacts with GPR30 and may execute its action by interaction with GPR30 [46] Interestingly, human alpha-fetoprotein peptides also binds to the estrogen receptor and estradiol, and...

Ngày tải lên: 16/03/2014, 18:20

... NaCl/Pi and solubilized A glutathione S-transferase (GSt) fusion of eRBCC was expressed in E coli and assessed for its ubiquitination Cell culture and plasmid transfection Ó FEBS 2002 RBCC protein expression ... each protein relative to eRBCC are shown under the name of the protein The identity and similarity of the B30.2 domains are also shown Proteins compared with eRBCC are PwA33 [23], Xnf7 [5], and ... amino acids on the lines, and the numbers to the left refer to the first nucleotides on the lines The putative initiation codon (ATG) and an upstream stop codon (TGA) are underlined Conserved cysteine/...

Ngày tải lên: 17/03/2014, 10:20

... intestinal cell line and that the amount of CRBPI and CRBPII mRNA and protein is directly related to the rate of retinol absorption It is believed that CRBPII directs retinal and retinol to the ... to Gln109 and Gln129 in CRBPI and CRBPII While all FABPs and CRBPs studied to date have the same tertiary structure, the amino-acid residues at positions 109 and 129 may determine ligand-binding ... CRBPII- and I-FABP-specific antisense and negative control I-FABP sense probes Transverse (A) and coronal (B) zebrafish sections were hybridized to the I-FABP, CRBPII and negative control probes and...

Ngày tải lên: 17/03/2014, 11:20

... opted to utilize continuous expression of the unmodified TetR The original tetracycline repressor simply binds to a tandem of the type tetracycline operator (tetO2) operator and thus blocks transcription ... contrast to expression of membrane proteins, the level of expression of cytosolic eGFP in the on-state was higher with pEBTet It follows that pEBTetD provides low background and moderate expression ... multiple effects on gene expression is avoided In summary, we have developed plasmid pEBTetD for full establishment of doxycycline-inducible protein expression in human cell lines by just a single...

Ngày tải lên: 23/03/2014, 09:21

... b-strands H and F To form the tetramer, two dimers associate back to back, mainly through hydrophobic contacts between residues of the loops formed by b-strands A and B and b-strands G and H ... polyene chain is fully extended and the hydroxyl end group is almost solvent exposed, in the region of the loops that connect b-strands A and B, C and D and E and F and surround the entrance of ... interaction between RBP and TTR (Table and Fig 2B), and some of them (I84S, D99A and S100E) are shown in the present study to impair or abolish protein protein recognition (Table and Fig 3A) The results...

Ngày tải lên: 30/03/2014, 02:20

... between zebrafish and human, mouse and pufferfish B-FABP is 83%, 76% and 83%, respectively The percentage amino acid identity between zebrafish and human and mouse is higher in the exons and than it ... lipid-binding proteins and their genes Annu Rev Nutr 17, 277–303 25 Wu, Q., Andolfatto, P & Haunerland, N.H (2001) Cloning and sequence of the gene encoding the muscle fatty acid binding protein from ... specific patterns of expression in developing neural tissues [33] and in the adult brain [34] B-FABP is specifically expressed in the mammalian and zebrafish brain [11,13,15,17], and its expression correlates...

Ngày tải lên: 31/03/2014, 07:20

... immunoreactivity band were observed, only the 80 kDa band showed up with strong intensity (B) and (C) Anti-GFP and anti-VRL-1 Western blot of F-11 cells expression VRL-1-GFP The same band pattern of ... four stable F-11 cell lines A–D Unique expression of mouse PB and MPI was observed in line C [7] As shown by Mevel-Ninio and Weiss [12] it cannot be excluded that some gene expression might be ... rVRL-1-GFP band appeared at  110 kDa, the upper band at 114 kDa and the diffuse band at 128 kDa The endogenous VRL-1 can also be observed at  80 kDa in the anti-VRL-1 Western blot and does not...

Ngày tải lên: 31/03/2014, 07:20

... relationship between polh protein expression and oxaliplatin sensitivity of gastric cancer cell lines and the significance of that in predicting treatment response and survival of metastatic ... cell lines Oxaliplatin sensitivity of four gastric cell lines (SGC7901, AGS, MKN45, and MGC803) were detected by MTT assay described above Endogenous polh protein expression of four cell lines ... in these four gastric carcinoma cell lines (SGC7901, AGS, MKN45, and MGC803) by MTT assay and protein expression by western blotting We found a significant linear relationship between them Our...

Ngày tải lên: 18/06/2014, 16:20

... as the ratio between the intensity of the protein of interest band and the loading control protein band (b-actin) Quantitative real time PCR (qPCR) mRNA expression was determined in mouse mixed ... proinflammatory gene expression in C/EBPb-/- mixed glial cultures Expression of pro-inflammatory (NOS2, IL-1b, IL-6 and TNFa) and anti-inflammatory (IL-4 and TGFb1) genes in C/EBPb+/+ (white bars) and C/EBPb-/- ... (n = 4-5) NOS2 protein levels by western blot and immunocytochemistry, and generation of NO by colorimetric detection of nitrites (Griess assay) In wild-type cultures NOS2 protein expression was...

Ngày tải lên: 19/06/2014, 22:20

... techniques in breast cancer cell lines [14] and ovarian cancer cell lines [15] and similar results have been obtained regarding the staining distribution in various normal and cancerous tissues (data ... reduced expression of RBM3 was observed in metastases compared to primary tumours, which is quite in line with previous in vitro data [20] Methods The Malmö Diet and Cancer Study The Malmö Diet and ... use of tobacco and alcohol, heredity, socio-economic factors, education, occupation, previous and current disease and current medication In addition, blood samples were collected and stored in...

Ngày tải lên: 20/06/2014, 04:20

... and chondrocyte cell lines results in increased apoptosis and caspase activity [43,44] Since we have previously shown that TNF-α increases PACT protein expression and phosphorylation of PKR and ... transcription and activation of MMPs via induction of NFκB and early response genes (c-fos and c-jun) Activation of PKR and subsequent phosphorylation of eIF2α would lead to an inhibition of protein ... glycerol, and 0.01% (w/v) bromophenol blue), a recombinant MMP-9 standard (1 µl) (Oncogene, Nottingham, UK), bovine-fibroblast-conditioned medium (5 µl) known to contain MMP-2 activity, and protein...

Ngày tải lên: 09/08/2014, 01:23

... both MMP-13 mRNA expression and the protein to an approximately similar extent Cathepsin K gene expression and protein synthesis The levels of both the gene expression and the protein of cathepsin ... correlation exists between the mRNA and protein levels At the two dosages tested, the R1094 ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis The level of gene expression of ADAMTS-5 in OA ... mechanisms of ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis are complex and may vary based on species and culture conditions [15] The present study found the level of expression of ADAMTS-5...

Ngày tải lên: 09/08/2014, 06:23

... serum and (b) arthritic joints and (c) TIARP mRNA and protein expression in spleens (left and middle panels) and arthritic joints (right panel) by real-time polymerase chain reaction (PCR) and ... and protein was noted at days 14 and 28, and the expression correlated with joint swelling (Figure 2c, right Figure Serial changes in expression levels of tumor necrosis factor-alpha (TNFα) and ... patients with RA and OA, we investigated the expression of STEAP4 mRNA in synovium of patients with RA and OA Relative expression of STEAP4 was almost comparable between RA and OA, although expression...

Ngày tải lên: 09/08/2014, 14:22

... IIa, IIx, and IIb) and while diffuse atrophy has been reported here and in the literature [18], the subtle morphological and genetic differences between the mouse and rat transgenic models and the ... plantaris muscles to detect levels of the thiol pairs GSH and GSSG, and Cys and Cyss HIV-1-related protein expression increased the levels of GSSG (B) and Cyss (E) compared to controls Surprisingly, GSH ... tissues to detect levels of the thiol pairs GSH and GSSG, and Cys and Cyss HIV-1-related protein expression had no effect on GSH or GSSG levels (A and B), but did increase the overall oxidative...

Ngày tải lên: 10/08/2014, 05:21