light oxygen and generation of reactive oxygen species
Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in ... amount of GSH in the samples was determined from a standard curve obtained by plotting known amounts (from 0.05 to 0.4 lgÆmL)1) of GSH vs the rate of change of absorbance at 412 nm GSH standards ... the CELLQUEST software program (Becton-Dickinson) Fig GSH and GSSG concentrations and ratio of GSH2 : GSSG in q+ and q0 cells from osteosarcoma (bone), rhabdomyosarcoma (muscle) and lung carcinoma...
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báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
Ngày tải lên :
20/06/2014, 00:20
... Medicine and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species ... modification of molecules and/ or disruption of cellular structures and tissue injury [9] Due to high MPO activity, the generation of hypochlorous acid (HOCl) and reactive nitrogen species (RNS) ... susceptibility of lipids to oxidation was used to calculate the sample size According to the mean comparison formula [15] with a standard deviation of 157.53 and a difference of 616, Za of 95% and a Zb of...
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Báo cáo y học: "Rheumatoid peripheral blood phagocytes are primed for activation but have impaired Fc-mediated generation of reactive oxygen specie" pptx
Ngày tải lên :
09/08/2014, 10:20
... Analysis of reactive oxygen species Analysis of the intracellular release of ROS was determined with DHR-123 Purified neutrophils were incubated with DHR123 before specific stimulation, and the ... neutrophils (R2 = 0.29, p = 0.06; Figure 2c) Page of 11 (page number not for citation purposes) Between 50 and 80% of naïve and between 50 and 90% of memory CD3-positive T lymphocyte cells reportedly ... or fMLP (data not shown) Measurements of neutrophil and systemic activation Expression and regulation of L-selectin Examination of the basal expression of L-selectin on neutrophils showed a trend...
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Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
Ngày tải lên :
21/02/2014, 01:21
... 1.52–1.53 1.50–1.54 1.50 1.54 No adduct 1.58 1.59 of cysteine of glutathione of of of of of N-acetylcysteine CoA lipoate ethanol formate of cysteine of glutathione Methyl• 2.28–2.29 1.93 1.70–1.73 ... and the significant contribution of E3 to the total flavin content of mitochondria [24] suggest that the potential input of complex-bound E3 to the mitochondrial production of reactive oxygen species ... OOH MethylO• MethylO• t-ButylO• of linoleic acid Methyl• of linoleic acid of DTT of glutathione of cysteine 1.59 ± 0.01 Matched to thiyl radicals Reactive oxygen species 1.6 ± 0.004 1.57 ± 0.01...
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neural preprocessing and control of reactive walking machines - manoonpong
Ngày tải lên :
12/06/2014, 12:06
... addition, the morphologies of a salamander and a cockroach capable of performing efficient locomotion are also taken into account for the leg and trunk designs of four- and six-legged walking machines, ... agent, i.e., the type and position of the sensors and the configurations of the actuators Choosing too simple a design, the behavior of the body may be of limited interest and it may obstruct the ... walking on land Each limb is formed of three main segments which are thigh, shank and foot (Fig 2.9) Fig 2.9 The limbs of a salamander (Reproduced with permission of G Nafis 2005 [139] (left) and K...
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Neural Preprocessing and Control of Reactive Walking Machines pdf
Ngày tải lên :
27/06/2014, 06:20
... addition, the morphologies of a salamander and a cockroach capable of performing efficient locomotion are also taken into account for the leg and trunk designs of four- and six-legged walking machines, ... agent, i.e., the type and position of the sensors and the configurations of the actuators Choosing too simple a design, the behavior of the body may be of limited interest and it may obstruct the ... walking on land Each limb is formed of three main segments which are thigh, shank and foot (Fig 2.9) Fig 2.9 The limbs of a salamander (Reproduced with permission of G Nafis 2005 [139] (left) and K...
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Báo cáo y học: "Aetiology and pathogenesis of reactive arthritis: role of non-antigen-presenting effects of HLA-B27" pdf
Ngày tải lên :
09/08/2014, 06:23
... efficiency and misfolding phenotype of the HLA-B27 HC, because the mutation of certain amino acid residues in the B pocket enhances the folding kinetics of the HLA-B27 HC and prevents the misfolding of ... slow, which leads easily to the generation of misfolded HLA-B27 HCs in the ER even in the normal presence of chaperones, β2m and peptide [17] Second, it is capable of forming aberrant disulphide-linked ... means of the Golgi machinery, where the carbohydrate residues of HCs are modified [17,20,21] The formation of a stable HC–β2m–peptide complex and the proper three-dimensional structure of the...
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Báo cáo y học: " A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions" pptx
Ngày tải lên :
13/08/2014, 13:20
... analysis of clones of GC4 infected by Akv-Y and Akv-2XY Figure Flow cytometry analysis of clones of GC4 infected by Akv-Y and Akv-2XY The profile of AYGC.2A2 was representative of all Akv-Y infected ... expressing Env and EYFP The profile of A2XYGC.2B1 was representative of all Akv-2XY infected GC4 clones, except A2XYGC.3B8, in that they expressed high EYFP levels and GFP-Cre but no Env Of all the ... between the 5'LTR and gag and between the env and the 3'LTR (b) The Akv-Y provirus contained IRES-EYFP in the U3 region of the LTR (c) The Akv-2XY provirus contained IRES-EYFP and two LoxP sites...
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Synthetic progress toward parvistemonine, spiroxins a and b, and generation of palmarumycin analogues
Ngày tải lên :
23/08/2015, 17:30
... the largest of the three groups and contains more than 25 species. 6 This family of plants has a history of medicinal applications Stemona and Croomia plants are used in both Japanese and Chinese ... advisor, Dr Wipf and the members of my committee: Dr Cohen, Dr Day and Dr Koide I would also like to thank the members of the Wipf group, both past and present Dr Stephen Lynch and Dr David Mareska ... Parvistemonine, Spiroxins A and B, and Generation of Palmarumycin Analogues Erika Elaine Englund, PhD University of Pittsburgh, 2008 Natural products can both challenge synthetic chemists and guide biologists...
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3 carbon isotope discrimination in roots and shoots of major weed species of southern u s rice fields and its potential use for analysis of rice–weed root interactions
Ngày tải lên :
04/09/2015, 07:24
... developed on the basis of knowledge of the carbon composition of the plants and soil With the exception of barnyardgrass, little is known of the suitability of major weed species to 13C isotope ... mass in each pot were extracted and cleaned thoroughly Expanded Species Survey in Field In 2007 and 2008, an expanded group of weed species was sampled from natural stands present in drill-seeded, ... analysis, often measured as 13 d C, an expression of the 13C/12C isotope ratio relative to a fixed standard, has been used previously to determine the proportions of roots of C3 and C4 species...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
Ngày tải lên :
05/03/2014, 23:20
... inability of MB and LPS to induce any impairment in mitochondrial function after h of treatment RCR and states 2, 3, and FCCP rates of MB- and LPStreated cells were the same as those of unstimulated ... cellular ROS and reactive nitrogen species (RNS) production The increased production of reactive species was apparent after h of stimulation Since UCP2 is described as a regulator of ROS production, ... mechanism of action of MDP on the mitochondrial bioenergetics system and since mitochondria are an important source of ROS production and especially of superoxide anion, we investigated the effect of...
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Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx
Ngày tải lên :
16/03/2014, 06:20
... chain of this residue forming hydrogen bonds with oxygens of the 3-phosphate; NH1 and NH2 of Arg105 form hydrogen bonds with the 4-OH and 5-OH of the inositol moiety; and the aromatic ring of Tyr59 ... a-helices of TPR1 and TPR2 (position of TPR2) and Asn204 in the b-hairpin (position 34 of TPR3), are highly conserved among p67phox and its related proteins (Fig 14) In addition, substitution of Glu ... regulation and evolution of Nox H Sumimoto Introduction Reactive oxygen species (ROS) are conventionally regarded as inevitable deleterious byproducts of aerobic metabolism On the other hand, there...
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Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps
Ngày tải lên :
09/08/2014, 08:23
... the nomenclature of free radicals, the term 'reactive oxygen species' (ROS) has prevailed, although ROS can be differentiated into reactive nitrogen species and other oxidant species The relationship ... expression of VEGF, VEGF-121 and VEGF-165 splice variants and VEGF receptors (VEGFR-1 and VEGFR-2) after stimulation by ROS donors Knowledge of this expression could lead to a better understanding of ... controls released 1,100 pg of VEGF per ml of medium and cartilage explant controls released 540 pg of VEGF per ml of medium Treatment with PMA at concentrations of 5, 10 and 20 μg/ml increased VEGF...
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báo cáo khoa học: " Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein" pps
Ngày tải lên :
11/08/2014, 11:22
... Maier M, Landthaler M, Szeimies RM, Baumler W: The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria Proceedings of the National Academy of Sciences of the ... 24h Figure Effect of high light treatment on plant growth and on gene expression at low temperature A) Phenotype of WT and npq1lut2 plants at time and 24 h under the high -light and low temperature ... individual detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo Plant Journal 2007, 50:475-487 88 de Torres M, Sanchez P, Fernandez-Delmond...
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báo cáo khoa học: " Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets" ppsx
Ngày tải lên :
12/08/2014, 03:20
... xenobiotic and ROS signalling are discussed Results Patterns of accumulation of singlet oxygen, superoxide radical and hydrogen peroxide The transfer of plantlets after weeks of growth to control and ... 57:711-738 Asada K: Production and scavenging of reactive oxygen species in chloroplasts and their functions Plant Physiol 2006, 141(2):391-396 Bowler C, Slooten L, Vandenbranden S, De Rycke R, Botterman ... detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo Plant J 2007, 50(3):475-487 Chao L, Zhou QX, Chen S, Cui S, Wang ME: Single and...
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Báo cáo y học: " Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads" potx
Ngày tải lên :
12/08/2014, 23:22
... levels of intracellular reactive oxygen species (ROS) using the 2'-7'-dichlorofluorescein diacetate (CM-H2DCFDA) probe The kinetics of ROS production after short term cultures (30 mn, h and h) ... Control % of 8-OHdG positive B cells Red CM-XROS 60 Profile of fluorescence intensities 50 40 30 20 10 Control Figure of Analysis DNA oxidative damage Analysis of DNA oxidative damage After 24 h of ... Figure Generation of ROS in B lymphocyte mitochondria Generation of ROS in B lymphocyte mitochondria Confocal microscopy analysis of ROS production in B cells isolated from BLV-infected and control...
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Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species
Ngày tải lên :
24/08/2014, 12:59
... SCHOOL Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species For the degree of Master of Science Choose your degree ... ROLE OF STAT3 AND REACTIVE OXYGEN SPECIES A Thesis Submitted to the Faculty of Purdue University by America Bethanne Newnum In Partial Fulfillment of the Requirements for the Degree of Master of ... University, August 2012 Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species Major Professor: Jiliang Li Signal Transducers and Activators of Transcription (STAT3), a transcription factor...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... and acts as a promoter for both the heavy and light strands of the mtDNA, and contains essential transcription and replication elements The D-loop region is a hot spot for mtDNA alterations and ... in the detection of absorbance The experiment was repeated times and data were expressed as Χ± SD Detection of CagA and VacA protein using SDS-PAGE and Western Blot Detection of cell viability ... microliters of extracts were mixed thoroughly with 20 μl of loading buffer, which were then boiled for Ten microliters of the mixture were subjected to SDS–PAGE, and bands were captured Treatments of...
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Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging doc
Ngày tải lên :
23/03/2014, 09:21
... dependency and the tissue specificity of Cygb and Ngb mRNA upregulation upon hypoxia and after reoxygenation In addition, we tried to shed light on the ROS-scavenging function of Ngb and on the ... upregulated upon 48 h of hypoxia, and that this increase is dependent on the tissue and the severity of hypoxia The mechanism of induction of Cygb is regulated by HIF-1 The aim of this study was ... factor (HIF)-1a and upregulation of Cygb mRNA, and hypothesized that Cygb (and Ngb) may have a vital role in retinal oxygen homeostasis, enabling the retina to sustain longer periods of ischemia...
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Báo cáo khoa học: Is there more to aging than mitochondrial DNA and reactive oxygen species? pot
Ngày tải lên :
29/03/2014, 22:21
... apyrimidinic endonuclease APE ⁄ Ref1; SSB and DSB, single-strand break and double-strand break, respectively ase, may aid in the generation of abasic sites and single-strand breaks This model provides a ... (1999) Effects of Ni(II) and Cu(II) on DNA interaction with the N-terminal sequence of human protamine P2: enhancement of binding and mediation of oxidative DNA strand scission and base damage ... (GPx1; Fig and Eqn 4) and peroxiredoxins III and V (PrxIII and PrxV; Fig and Eqns and 6, respectively; [55]) At least seven GPx enzymes have been described to date in mammalian cells [56], and two...
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