ligands shed receptors and endothelial cells
báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc
Ngày tải lên :
20/06/2014, 01:20
... these cells seeded on it Figure tion Effects of composite scaffolds on the cells viability and funcEffects of composite scaffolds on the cells viability and function The induced osteoblasts and endothelial ... for cell process and spreading, adhesion and orientation Therefore, osteoblasts and endothelial cells were inclined to attach to the rougher surface and appeared better viability and strong cellular ... (arrows), and cellular projection (1500×) Panels (C) and (D) revealed the growth of endothelial cells on a PCL-HA scaffold The Panel (C) pictured ECs proliferating and forming a cell sheet (600×), and...
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![Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc](https://media.store123doc.com/images/document/2014_08/12/medium_9S3XH52qK4.jpg)
Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc
Ngày tải lên :
11/08/2014, 08:21
... Materials and Methods section (n = 3) Effects of NF161 and NF177 on PGE2 and PGI2 production by pre-induced COX-2 IL-1α (10 ng/ml) was added and incubated for 20 h The cells were washed and NF161 ... was 51 ± cells/ microscope fields (n = 30) The direct effect on HUVEC was assessed by pretreated the cells with the 1, 8-naphtyridines (10-4M) and 10-7M PAF for 20 min, washed three times and challenged ... washed with BSS, and plated at 106 cells/ well in a final volume of 0.25 ml BSS on HUVEC pretreated with the 1, 8-naphtyridines (10-6–104M) for 10 and challenged with various stimuli: FMLP and...
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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf
Ngày tải lên :
19/06/2014, 22:20
... endothelial cells and inhibits adhesion and transendothelial migration of HIV-1 infected monocytes J Immunol 2008, 180:1854-1865 47 Craig LE, Spelman JP, Strandberg JD, Zink MC: Endothelial cells ... VCAM-1 expression in brain endothelial cells infected Page of 13 with TMEV and the possible receptors involved by using antagonists of the classical cannabinoid receptors, CB1 and CB2, antagonists ... antibiotic penicillin and streptomycin (all from Gibco, Scotland, UK) and were maintained under standard cell culture conditions at 37°C and 5% CO2 One hour before experiments, cells were subjected...
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Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor" doc
Ngày tải lên :
11/08/2014, 08:20
... http://www.jnrbm.com/content/5/1/8 cells Undifferentiated PC12 cells were known to express VEGF to stimulate angiogenesis [23] We now demonstrate that proliferating and differentiated PC12 cells also express VEGFR-1 and -2 and ... Cheng HJ, Feldheim DA, Hattori M, Lu Q, Vanderhaeghen P: Alkaline phosphatase fusions of ligands or receptors as in situ probes for staining of cells, tissues, and embryos Methods Enzymol 2000, 327:19-35 ... VEGF and endostatins To determine the expression profile of binding partners for murine VEGF164, endostatin and the collagen XV endostatin homologue on neuronal and endothelial cell PC12 cells...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
Ngày tải lên :
14/02/2014, 14:20
... intact cells S A Mousavi et al Fig Dissociation time courses and effects of unlabeled ligands and low pH on the dissociation rates of [125I]TC-PCSK9 (A) and [125I]TC-PCSK9-D374Y (B) from HepG2 cells ... the cells were washed four times with wash buffer as described above, and then incubated in fresh binding medium At various times, cells were washed once with mL of cold DMEM and solubilized, and ... 15 Labeled ligand was added in a small volume (20 lL) and the cells were incubated at °C for h At the end of incubation, the cells were washed once with mL of cold wash buffer and three times...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
Ngày tải lên :
18/02/2014, 17:20
... cells were suspended with 500 lL MACS buffer and applied onto MACS columns Magnetically selected positive cells and depleted cells were cultured independently as lymphatic endothelial cells and ... murine endothelial cells from various developmental stages and locations is important for dissecting molecular and cellular mechanisms of endothelial development and function Murine primary cells, ... dishes and cultured at 33 °C (day in Fig 1) For the initial weeks, primary cells, including both tsA58T Ag-negative cells (primarily fibroblasts) and tsA58T Ag-positive cells, proliferated, and ECs...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
Ngày tải lên :
20/02/2014, 11:20
... constitutively expressed fgl2 at low levels in cells or tissues including cytotoxic T cells, trophoblasts, intestinal epithelial cells and endothelial cells remains to be defined We hypothesize that ... structural and functional characterization of the 5¢-flanking region of the mouse fgl2 gene in vascular endothelial cells Through a series of studies focusing on constitutive expression in endothelial cells, ... loading and first strand synthesis with forward primer, 5¢-ATGTTTGAGACCTTCAACAC-3¢, and reverse primer, 5¢-CACGTCACACTTCATGAT GGA-3¢ then excised from PCR2.1 by digestion with HindIII and SalI and...
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Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf
Ngày tải lên :
16/03/2014, 18:20
... vein endothelial cells (HUVECs) and rabbit coronary endothelial cells have reported a redistribution of cPLA2-a to the nuclear envelope [26,27] The localization of cPLA2-a in human endothelial cells ... above and with those seen 1281 cPLA2-a localization in endothelial cells A S Grewal et al B C Fig The location and relocation of cPLA2-a in Triton X-100- and digitonin-permeabilized cells (A) Cells ... cPLA2-a expression levels and subcellular distribution in EA.hy.926, HUVEC, A549 and HeLa cells (A) Total cell lysates from EA.hy.926, HUVEC, A549 and HeLa cells were prepared and 20 lg protein of...
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Báo cáo khoa học: Lithium increases PGC-1a expression and mitochondrial biogenesis in primary bovine aortic endothelial cells ppt
Ngày tải lên :
23/03/2014, 09:20
... vein endothelial cells [15], vascular smooth muscle cells [13], lens epithelial cells [16], and some tumor cell lines such as B16 melanoma [17] and P19 embryonal carcinoma cells [18] Both GSK3b and ... biogenesis and mass within cells and tissues reflect changes in energy demand and hormonal status, and depend mainly on changes in the activity and ⁄ or expression of the transcription factors NRF1 and ... cerebellar cells [19] and enrichment of active GSK3b in mitochondria and nuclei has also been observed in neuronal cells [20] In the immortalized neuronal cell line SH-SY5Y, mitochondrial and active...
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Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf
Ngày tải lên :
30/03/2014, 01:20
... amplify the inflammatory response in endothelial cells In the present study, we show that stimulation of human endothelial cells with a combination of AGEs and LPS demonstrates a synergistic effect ... AGE-HSA and LPS amplified inflammatory response Furthermore, blockade of both MAP kinases and NF-jB with a combination of inhibitors completely abolished AGE-HSA and LPS costimulated IL-6, IL-8 and ... effect of AGEs and LPS on endothelial cells, and, furthermore, the underlying signal mechanisms involved, have not been fully understood In the present study, we found that both AGE-HSA and LPS, in...
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Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx
Ngày tải lên :
30/03/2014, 13:20
... Afterwards, the medium was aspirated and the cells washed three times with ice-cold 0.1 M MgCl2 to stop pump activity and remove excess 86Rb+ The washed cells were then disrupted by treatment ... concentration of 100 nM and incubation was continued for another 30 Afterwards, the medium was removed by aspiration, and cells were washed twice in 500 lL ice-cold water Then cells were dissolved ... treatment Ouabain was added to the cells at various concentrations in fresh ECGM0.5, and the cells incubated for an additional 12 h For counting, cells were detached and dispersed in trypsin/ EDTA...
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báo cáo hóa học:"Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells" pdf
Ngày tải lên :
18/06/2014, 15:20
... washed, and Ca2+ free buffer containing IgG (panel A), the CD66ae mAb and CD66b mAb (panel B), the CD66ae mAb and CD66c mAb (panel C), the CD66ae mAb and CD66de mAb (panel D), the CD66b mAb and ... CD66ae and CD66de mAbs, but they remained responsive to CD66b, CD66c, and CD63 mAbs (Fig 1, panel D) When cells were desensitized to CD66b and CD66c mAbs, the cells were unresponsive to CD66b and ... other cells expressing CEACAMs CEACAM1 and CEACAM6 have been reported to present selectin ligands to CD62E (ELAM-1, E-selectin) on endothelial cells [23], and appear to be involved in angiogenesis...
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báo cáo hóa học: " All-trans retinoic acid induces COX-2 and prostaglandin E2 synthesis in SH-SY5Y human neuroblastoma cells: involvement of retinoic acid receptors and extracellular-regulated kinase 1/2" potx
Ngày tải lên :
19/06/2014, 22:20
... 89:1291-300 Thacher SM, Vasudevan J, Chandraratna RAS: Therapeutic applications for ligands of retinoid receptors Therapeutic applications for ligands of retinoid receptors Curr Pharm Design 2000, ... and activate retinoid nuclear receptors, including retinoic acid receptors (RARs) and/ or retinoid X receptors (RXRs), each having three isotypes, α, β and γ RARs and RXRs have been identified ... expression and increases PGE2 in SH-SY5Y human neuroblastoma cells Figure ATRA up-regulates COX-2 expression and increases PGE2 in SH-SY5Y human neuroblastoma cells (a,b) Expression of COX-1 and COX-2...
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Báo cáo y học: "Myeloid dendritic cells display downregulation of C-type lectin receptors and aberrant lectin uptake in systemic lupus erythematosus" pdf
Ngày tải lên :
09/08/2014, 13:22
... microbes, as well as ligands expressed on apoptotic and malignant endogenous cells Additionally, they can interact with other pattern recognition receptors such as Toll-like receptors DCs express ... processing, and flow cytometry was performed as for the FD experiments Immunofluorescence staining and flow cytometry DCs were washed with PBS/0.2% BSA, and Fc receptors were blocked by incubating cells ... dendritic cellspecific HIV-1-binding protein that enhances trans-infection of T cells Cell 2000, 100:587-597 Bajtay Z, Csomor E, Sandor N, Erdei A: Expression and role of Fc- and complement-receptors...
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báo cáo khoa học: " Silver nanoparticles inhibit VEGF-and IL-1 -induced vascular permeability via Src dependent pathway in porcine retinal endothelial cells" potx
Ngày tải lên :
11/08/2014, 00:22
... Ag-NP and PP2 on VEGF -and IL-1β-induced Src phosphorylation Effect of8 Effect of Ag-NP and PP2 on VEGF -and IL-1 -induced Src phosphorylation PRECs were treated with VEGF and IL-1β in presence and ... the permeability effect of VEGF and IL-1β Figure permeability Ag-NP inhibits the VEGF -and IL-1β-induced endothelial cell Ag-NP inhibits the VEGF -and IL-1 -induced endothelial cell permeability ... Biosystems) and cell viability determined by trypan blue exclusion was about 90% Transwell monolayer permeability assay To measure solute flux across endothelial cells, retinal endothelial cells were...
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Báo cáo y học: "Endotoxin-activated microglia injure brain derived endothelial cells via NF-B, JAK-STAT and JNK stress kinase pathways" docx
Ngày tải lên :
11/08/2014, 06:22
... microglia are toxic to endothelial cells, and in particular, targeting the JAKSTAT pathway in microglia would confer protection of both endothelial cells and microglia, and prevent microglial ... bEND.3 and BV2 cells were prepared and exposed to LPS for 24 h Immunostains of cell type markers showed that endothelial cells (Panel B, CD33, green) were primarily affected compared to BV2 cells ... stimulate microglia, but not endothelial cells LPS also directly induced cell death in microglia, but not endothelial cells However, LPS could only injure endothelial cells when cocultured with...
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Báo cáo y học: "Protein Never in Mitosis Gene A Interacting-1 regulates calpain activity and the degradation of cyclooxygenase-2 in endothelial cells" pot
Ngày tải lên :
11/08/2014, 08:22
... comparison between medium- and LPS/IFN-treated cells, and +, p < 0.05 for comparison between KD and Control cells treated in the same manner tures selected for the KD and Control shRNA (data not ... LPS/IFN- and medium-treated cells +: p < 0.05 for comparison between KD and Control cells treated with LPS/IFN and zVF tested Like zVF, PD 150606 increased the induction of COX-2 in KD and Control ... primary and secondary stimuli that may be present, and by cellular factors, such as supply of arachidonic acid, complement of various prostaglandin synthases, and expression of prostaglandin receptors...
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Báo cáo y học: "Nipah virus infection and glycoprotein targeting in endothelial cells" ppsx
Ngày tải lên :
12/08/2014, 02:20
... polarized endothelial cells causes syncytia formation and increases transendothelial permeability Primary brain capillary endothelial cells have the closest resemblance to brain endothelia in vivo and ... lysis and immunoprecipitation, F and G proteins were separated by SDS-PAGE and blotted to Table Summary and comparison of NiV infection and glycoprotein targeting in polarized epithelial and endothelial ... panel (C) Cells were fixed and surface-stained from both sides with a EB2-specific ligand (EphB4/Fc) and a AlexaFluor 568-labelled secondary antibody Then cells were permeabilized and incubated...
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Báo cáo khoa học: " Profile of time-dependent VEGF upregulation in human pulmonary endothelial cells, HPMEC-ST1.6R infected with DENV-1, -2, -3, and -4 viruses" pps
Ngày tải lên :
12/08/2014, 04:21
... and infected HPMEC-ST1.6R cells RT-PCR analysis of DENV-1, -2, -3 and infected HPMEC-ST1.6R cells Cell culture media of HPMEC-ST1.6R cells infected with DENV-1, -2, -3 and -4 were collected and ... "primed" endothelial state would be greatly enhanced due to a cascade of cytokines secreted by other infected neighboring target cells such macrophages and dendritic cells [2,4,14], apart from the endothelial ... in 5% CO2 for days The cell media of DENV-infected cells and mock-infected cells were collected at 0, 8, 24, 96 and 192 hours post-infection, and analyzed for VEGF production [actual values listed...
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Báo cáo y học: " GDF-15 is abundantly expressed in plexiform lesions in patients with pulmonary arterial hypertension and affects proliferation and apoptosis of pulmonary endothelial cells" ppsx
Ngày tải lên :
12/08/2014, 13:22
... normal lung, GDF15 was noted in endothelial cells of small pulmonary arteries as well as in alveolar macrophages (Figure 2) Smooth muscle cells and epithelial cells exhibited only a weak signal ... the staining of endothelial cells in small pulmonary arteries (arrow) Insets depicts a high-power view, highlighting the expression of GDF-15 in endothelial cells Smooth muscle cells exhibit a ... significant inhibition of endothelial cell proliferation (Figure 11, panel B) Figure Upregulation of GDF-15 by hypoxia in endothelial cells Human pulmonary microvascular endothelial cells (HPMEC) were...
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