lglutamate oxidase lgox from streptomycessp x1196

... Inhibitor-binding sites of L-amino acid oxidase FEBS Journal 275 (2008) 2078–2095 ª 2008 The Authors Journal compilation ª 2008 FEBS 2087 Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia ... L-amino-acid oxidase from the Malayan pit viper Calloselasma rho- dostoma. Eur J Biochem 268, 4044–4053. 24 Coles CJ, Edmondson DE & Singer TP (1977) Reversible inactivation of L-amino acid oxidase. ... markers (29–207 kDa) were from Sigma-Aldrich (St Louis, MO, USA). Sephadex G-100, Sephadex G-75 and CM- Sephadex C-50 were from Amersham Biosciences (Uppsala, Sweden). Peroxidase (horseradish, specific...

Ngày tải lên: 07/03/2014, 05:20

... hyperthermophilic archaeal-type metallo -oxidase, designated McoP (multicopper oxidase from P. aerophilum). Indeed, whereas MCOs, both laccases and metallo-oxidases, are well characterized in eukaryotes ... the metallo -oxidase from Aquifex aeolicus. Biochim Biophys Acta 1794, 75–83. 5 Fernandes AT, Soares CM, Pereira MM, Huber R, Grass G & Martins LO (2007) A robust metallo -oxidase from the hyperthermophilic ... focused our attention on the study of prokaryotic MCOs, the CotA laccase from Bacillus subtilis and the metallo -oxidase McoA from Aquifex aeolicus, because of their potential for biotech- nological...

Ngày tải lên: 18/02/2014, 04:20

... Si-face stereospecificity at C5 of coenzyme F 420 for F 420 H 2 oxidase from methanogenic Archaea as determined by mass spectrometry Henning Seedorf 1 ,Jo ¨ rg Kahnt 1 , ... unrelated phylogenetically. In this study, we report that the recently discovered F 420 H 2 oxidase from methanogenic Archaea is also Si-face stereospecific. The enzyme was found to catalyse the ... the deter- mination of the stereospecificity of F 420 H 2 oxidase (FprA). The insets show normalized 1 Da separated stick spectra obtained from the measured data (black) aligned to simulated spectra...

Ngày tải lên: 20/02/2014, 03:20

... cytochrome oxidase were investi- gated with optical and FT-IR spectroscopy: it acts as acceptor of electrons transferred from prereduced cyto- chrome bo 3 , the heme-copper oxidase from Escherichia ... of cytochrome bo 3 oxidase under quasi-physiological conditions. Keywords: cytochrome oxidase; caged compound; FT-IR spectroscopy; oxygen, l-peroxo cobalt complex. Cytochrome oxidases are hetero-oligomeric ... acceptor substrate, molecular dioxygen [3]. Cytochrome bo 3 oxidase of Escherichia coli is a ubiquinol oxidase. It transfers electrons fromthemembranesiteviahemeb to the binuclear reaction center,...

Ngày tải lên: 21/02/2014, 15:20

... cytochrome c oxidase from bovine heart and P. denitri- ficans, and the aberrant ba 3 -oxidase from T. thermophilus. MATERIALS AND METHODS Sample preparation The caa 3 -type cytochrome c oxidase from T. ... in direct compari- son to other oxidases, as for example the cytochrome c oxidase from P. denitrificans. In the spectral range from 2200–2000 cm )1 contributions from cyanide ligand bound to heme ... spectra, contributions from the porphyrin ring, the heme propio- nates and the vinyl substituent can be expected, originating from heme c, with contributions from the formyl groups and from the geranyl...

Ngày tải lên: 21/02/2014, 15:20

... pyranose 2 -oxidase; PDB, Protein Data Bank; PsP2Ox, pyranose oxidase from Peniophora sp.; TLS, translation, libration, screw-rotation; T m , melting temperature; TmP2Ox, pyranose oxidase from Trametes ... pyranose 2 -oxidase from the white-rot fungus Peniophora sp. Biochemistry 45, 6587– 6595. 7 Leitner C, Volc J & Haltrich D (2001) Purification and characterization of pyranose oxidase from the ... Pyranose 2 -oxidase from Phanerochaete chrysos- porium – further biochemical characterisation. Appl Microbiol Biotechnol 47, 508–514. 11 Volc J & Eriksson K-E (1988) Pyranose 2 -oxidase from Phanerochaete...

Ngày tải lên: 07/03/2014, 03:20

... eugenol oxidase was identified in the genome from Rho- dococcus sp. strain RHA1. The bacterial FAD-containing oxidase shares 45% amino acid sequence identity with vanillyl alcohol oxidase from the fungus ... basis of the increase in oxidase activity. Discussion In this study, a new bacterial oxidase was cloned and characterized: EUGO from Rhodococcus sp. strain RHA1. The oxidase shows sequence identity ... alcohol oxidase structure indicates that the FAD cofactor is tethered to His390 in eugenol oxidase. The model also provides a struc- tural explanation for the observation that eugenol oxidase...

Ngày tải lên: 07/03/2014, 09:20

... F 420 H 2 oxidase from Methanothermobacter marburgensis, rubredoxin:NO ⁄ O 2 oxidoreductase from Desulfovibrio gigas, rubredoxin: NO ⁄ O 2 oxidoreductase from Moorella thermoacetica, F 420 H 2 oxidase ... O 2 oxidoreductase from D. gigas,in contrast to the rubredoxin:NO ⁄ O 2 oxidoreductase from Mo. thermoacetica [28] and F 420 H 2 oxidase. A third enzyme state was tentatively extracted from the electron ... F 420 H 2 oxidase from M. marburgensis is strictly conserved in other FprA enzymes from methanogenic Archaea (supple- mentary Fig. S1), most of which contain at least one FprA with F 420 H 2 oxidase...

Ngày tải lên: 07/03/2014, 10:20

... the three-dimensional structures of XDH and XO from several sources [27–30]. This review focuses mainly on the structural and functional changes of XOR resulting from its conver- sion from the XDH to the XO form, ... Della Corte E (1969) The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem 244, 3855–3863. 12 Della ... (1972) The regulation of rat liver xanthine oxidase. Involvement of thiol groups in the conversion of the enzyme activity from dehydroge- nase (type D) into oxidase (type O) and purification of the...

Ngày tải lên: 16/03/2014, 06:20

... purification of wild-type hexose oxidase and mutant H79K hexose oxidase, both from H. polymorpha. References 1 Hansen OC & Stougaard P (1997) Hexose oxidase from the red alga Chondrus crispus. ... choline oxidase; Fl(), position on the isoalloxazine ring; GOOX, glucooligosaccharide oxidase; HOX, hexose oxidase; MeCN, acetonitrile; MSOX, monomeric sarcosine oxidase; MTOX, N-methyltryptophan oxidase; ... type (WT) hexose oxidase (HOX) from H. polymorpha (o) shows low, pH-independent fluorescence emission at 525 nm, as does a trypsin-released flavopeptide from HOX (), contrary to COX from A. globiformis...

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