ledgf p75 is not essential for hiv 1 infection
Báo cáo y học: "Antiviral activity of a-helical stapled peptides designed from the HIV-1 capsid dimerization domain" pps
... (μM)in PBMC NYAD-2 01 >11 5 >11 5 NYAD-202 30.2 ± 4.32 >11 6 NYAD-203 13 .24 ± 0.5 15 .96 ± 1. 47 virions with NYAD-2 01 prior to infection, treated the target cells before or after infection, or treated ... BaL B PBMC R5 4.73 ± 1. 92 2.23 ± 0.44 89.6 B PBMC R5X4 5. 21 ± 0.87 3.47 ± 0.22 RT-Resistant Isolate AZT-R B MT-2 X4 8.0 ± 1. 27 4.53 ± 1. 19 PR-Resistant Isolate HIV- 1RF/L-323 -12 -3 B MT-2 X4 5.6 ... infectivity [19 , 31- 37] The isolated CTD of HIV- 1 CA, like full-length CA, forms a dimer in solution It has been shown that CTD dimerization is a major driving force in Gag assembly and maturation [10 ,15 ]...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... used for site-directed mutagenesis Desired mutation Template gene Constructed mutant Primer sequence (5Â- to 3Â) Restriction site W11F W168F WT* Y74W* WT WT W11F WT* W11F W168F W11F W168F W11F ... with those for wild-type PfTIM, yeast and GlTIM Enzymes kcat (ã 10 5 min )1) a Km (mM) kcat Km (ã 10 5 min )1 mM )1) References PfTIM WT GlTIM Yeast W11F W168F W11F W168F (WT*) Y74G W11F W168F Y74W ... 4.0 TWT W168F 330 2.5 40 àM 20 àM 10 àM àM 2.0 1. 5 W11F 325 10 20 30 Enzyme concentration (àM) 1. 0 40 10 0 200 300 400 500 600 700 800 900 10 00 11 00 Acrylamide concentration (mM) W11F/W168F/Y74W...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... ETD-MS analysis of peptides and proteins N D Udeshi et al A % Relative Abundance 10 0 200 400 600 800 10 00 m/z 12 00 14 00 16 00 18 00 2000 B 10 0 ETD-enabled LTQ-Orbitrap % Relative Abundance 10 0 % Relative ... analysis Biochim Biophys Acta 17 64, 18 11 18 22 Zubarev RA, Kelleher NL & McLafferty FW (19 98) Electron capture dissociation of multiply charged protein cations A nonergodic process J Am Chem Soc 12 0, ... 44, 13 202 13 213 17 Zhang Y (2003) Transcriptional regulation by histone ubiquitination and deubiquitination Genes Dev 17 , 2733–2740 18 Strahl BD & Allis CD (2000) The language of covalent histone...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... M.I., Askenase, P.W & Pober, J.S (19 97) Ó FEBS 2004 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Design of peptides for T-cell adhesion inhibition (Eur J Biochem 2 71) 2885 Blockade of CD2–LFA)3 ... function Peptides 21, 11 61 11 67 Supplementary material The following material is available from http://blackwell publishing.com/products/journals/suppmat/ejb/ejb 419 8/ ejb 419 8.htm Appendix Chemical ... PenERGSTLVAEFC Cyclo (1, 12) PenERGSTLVAEFC PenVYSTNGTRILC Cyclo (1, 12) PenVYSTNGTRILC Cyclo (1, 6) ERGSTL Cyclo (1, 6) YSTNGT KGKTDAISVKAI 36–45 36–45 85–94 85–94 36– 41 85–90 91 80a a Sequence from...
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Báo cáo khóa học: Antimicrobial activities of heparin-binding peptides pdf
... ARKKAAKA 11 .0 11 .0 10 .0 11 .0 12 .0 12 .0 12 .8 12 .8 12 .0 10 .0 10 .5 10 .3 12 .2 9.8 8.7 12 .5 12 .3 12 .0 11 .2 12 .3 12 .0 11 .3 [43] [43] [44] [45,46] [47] [48] [49], this report [49], this report [49] [50] [ 51] ... 50 SRN16 SRN29 KDF15 SAV15 RIQ17 PPP25 LGT25 RLR22 PGR 11 QPP18 YIG23 SEK20 AKK15 10 40 14 22 40 22 13 38 86 77 71 65 75 93 81 85 92 86 59 80 92 10 1 LRK26 LGE27 0,5 70 40 AKK24 AKK18 AKK12 AKK6 ... This report This report [15 ,20] [15 ,20] [15 ,20] [15 ,20] [15 ,20] [15 ,20] [15 ,20] GHR18 LVT19 GHRPLDKKREEAPSLRPA (15 –32) LVTSKGDKELRTGKEKVTS( 414 –432) 10 .0 9.5 Negative, this reportb Negative, this...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... electrophoresis Numbers in parentheses are minor components of the samples Peptides a-PEC Crystals of a-PEC Peptide 1A Peptide 1B Peptide 1C Peptide Peptide Molecular mass 18 15 1.6 18 18 15 (15 15 15 1.6 16 7.8 ... ammonium peroxodisulfate [17 ] Samples of 1. 5 mL containing 10 15 mgÆmL )1 protein were electrophoresed in the Tris/boric acid buffer system for 2400 VÆh )1 at a constant power of 18 W, at 10 °C and continuous ... the PEC complexes [11 ,12 , 21] The assembly of ‘monomeric’ and ‘trimeric’ complexes is accompanied by a decrease of the photochemistry from 10 0% of the isolated a-PEC to 8% for the ‘trimers’ Naturally,...
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Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx
... equilibrated for 10 in NaCl/Tris before permeabilization with 0 .1% Triton X -10 0 in NaCl/Tris for 30 at room temperature One hour preincubation was performed in the presence of 1% NGS and 1% BSA to ... (1. 5 lgÆmL )1) [14 ], was applied for 12 16 h at room temperature in NaCl/Tris/ NGS/BSA After washing three times (10 min) in NaCl/ Tris, cells were incubated for h at room temperature with : 10 0 ... Tris/NGS/BSA Grids were then washed three times for 10 with NaCl/Tris, postfixed for in NaCl/Tris containing 1% glutaraldehyde and washed twice for with distilled water Sections were stained for...
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Báo cáo khoa học: Non-hydrolytic functions of acetylcholinesterase The significance of C-terminal peptides pptx
... et al 14 12 10 –8 –6 40 –4 Log [T14] M 10 µM T14 ** T14 BTX + nM T14 BTX 10 µM 50 ** 80 0 .1 µM µM 10 0 12 0 0 .1 µM µM 10 µM Percentage ACh response 15 0 16 0 µM b a7 10 nM T14 10 µM a Organotypic ... 200 0 .1 µM C Xenopus oocytes Control nM mM BTX + nM T14 c 10 T3 T1 10 M M M M IV M 10 10 M LA 10 ro C on t M h 10 µM BuChE peptide l 10 µM BuChE peptide M 12 0 s Cultured GH4-h Cells 11 0 10 0 90 ... 200 10 0 0 .15 0 .1 0.05 0 T14 T30 [peptide] mM 1/ [ATC] [1/ mM] 20 16 12 Reaction rate Reaction rate C *** * *** * * 10 15 Fractions 20 25 40 30 n.s 20 10 0 10 15 Fractions 20 25 Fig Effects of T14...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... represented by the Minister of Agriculture and Agri-Food Canada Journal compilation ª 2008 FEBS 2407 A 0.5 :1 1 :1 2 :1 3 :1 4 :1 5 :1 0.5 :1 A Chugh and F Eudes 1: 1 2 :1 3 :1 4 :1 5 :1 CPPs and their cargo ... Trembleau A, Brunissen A, Chassaing A & Prochiantz A (19 96) Cell internalization of the third helix of the antennapedia homeodomain is receptor dependent J Biol Chem 2 71, 18 188 18 193 10 Henriques ... mediated by cell-penetrating peptide pep -1 into lipid vesicles and human HeLa cells is driven by membrane electrostatic potential Biochemistry 44, 10 189 10 198 11 Mano M, Teodosio C, Paiva A, Simoes...
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Báo cáo khoa học: Zinc potentiates the antibacterial effects of histidine-rich peptides against Enterococcus faecalis pot
... 29 212 80 BD 312 ATCC 29 213 59.6 ± 6.7 28.5 ± 3.8 78 .1 ± 10 .6 0 69.3 ± 10 .0 28.9 ± 6.3 63.3 ± 9.9 0 64.6 ± 6.4 12 .1 ± 2.7 56.0 ± 5.6 0 45.2 ± 14 .3 9.4 ± 3.7 35.4 ± 8.3 0 37.4 47 .1 ATCC 25922 27 .1 ... radiolabelled heparin ( 10 lgÆmL )1, 0.4 · 10 6 cpmÆlg )1) for h at room temperature in 10 mm Tris, pH 7.4 with or without 50 lm Zn2+ The membranes were washed for · 10 in 10 mm Tris, pH 7.4 A Bas 2000 ... different strains of E faecalis, 10 0 lm AHH24 :1 or AHH24:2 were incubated with E faecalis 2374, E faecalis BD 33 ⁄ 03, E faecalis BD 96 ⁄ 03 or E faecalis ATCC 29 212 in 10 mm Tris, pH 7.4 in the absence...
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Báo cáo khoa học: Multifunctional host defense peptides: functional and mechanistic insights from NMR structures of potent antimicrobial peptides docx
... functions Biochim Biophys Acta 17 58, 14 99 15 12 Hancock REW (20 01) Cationic peptides: effectors in innate immunity and novel antimicrobials Lancet Infect Dis 1, 15 6 16 4 10 Zasloff M (2002) Antimicrobial ... peptides Biochim Biophys Acta 17 58, 14 08 14 25 13 Epand RM & Vogel HJ (19 99) Diversity of antimicrobial peptides and their mechanisms of action Biochim Biophys Acta 14 62, 11 –28 14 Selsted ME & Ouellette ... organisms Nature 415 , 389–395 11 Oppenheim JJ, Biragyn A, Kwak LW & Yang D (2003) Roles of antimicrobial peptides such as defensins in innate and adaptive immunity Ann Rheum Dis 62, 17 – 21 12 Durr...
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Báo cáo khoa học: Effect of the -Gly-3(S)-hydroxyprolyl-4(R)-hydroxyprolyltripeptide unit on the stability of collagen model peptides ppt
... is catalyzed by the enzyme prolyl-3-hydroxylase (P3H1, EC 1. 14 .11 .7), which has three family members in vertebrates, but characterization of these enzymes is very limited [11 – 13 ] The analysis ... trimer) DS° (JÆ°C )1 mol )1 trimer) Tm (°C)a )3 21 )224 )17 7 )17 0 )15 8 )15 6 )8 81 )5 81 )439 ) 418 )373 )370 52.0 52.8 51. 0 52.6 56.2 55.3 a Tm (°C) ΔS (J·K 1 mole 1 trimer) Temperature (°C) modification lowered ... even for the same tissue and species [15 17 ] This is not just due to the heterogeneity of the modification, but also to differences in sample preparation for amino acid analysis As a result of this,...
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Báo cáo khoa học: Grafting of thrombopoietin-mimetic peptides into cystine knot miniproteins yields high-affinity thrombopoietin antagonists and agonists pot
... USA 10 1, 18 16 18 21 Methia N, Louache F, Vainchenker W & Wendling F (19 93) Oligodeoxynucleotides antisense to the protooncogene c-mpl specifically inhibit in vitro megakaryocytopoiesis Blood 82, 13 95 14 01 ... knot with agouti-related protein activity Biochemistry 41, 7565–7572 12 Craik DJ, Daly NL & Waine C (20 01) The cystine knot motif in toxins and implications for drug design Toxicon 39, 43–60 13 ... the cyclic cystine knot framework: implications for biological activity and drug design Biochem J 394, 85–93 14 Isaacs NW (19 95) Cystine knots Curr Opin Struct Biol 5, 3 91 395 15 Kaushansky K, Lok...
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Báo cáo khoa học: Bilayer localization of membrane-active peptides studied in biomimetic vesicles by visible and fluorescence spectroscopies pptx
... (ns)a SR (%)b Dm (cm )1) c None KAL Melittin Magainin II None KAL Melittin Magainin II 1. 3 1. 4 1. 7 1. 6 0.9 1. 2 1. 8 2.4 97 91 95 94 71 85 87 10 0 3480 3330 3 310 3290 3460 3200 3020 3 010 a Fig Evolution ... translocation Biochemistry 35, 11 3 61 11 368 18 Ladokhin, A.S & White, S.H (20 01) ÔDetergent-likeÕ permeabilization of anionic lipid vesicles by melittin Biochim Biophys Acta 15 14, 253–260 19 Ladokhin, ... Biopolymers 64, 314 –327 10 Blondelle, S.E & Houghten, R.A (19 91) Hemolytic and antimicrobial activities of twenty four individual omissions analogues of melittin Biochemistry 30, 46 71 4678 11 Lee, T.H.,...
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Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx
... 10 80.6 19 77.0 13 35.7 13 48.7 32.8 28.5 264.4 29.9 15 00.2 ± ± ± ± ± HPLC -1 1.3 1. 3 29.5 3.2 14 9.3 HPLC-2 HPLC-3 Observed [M + 2H]2+ (m/z) – 33.7 – 36.78 36 .12 41. 85 42.79 44.42 – – 54. 91 56.42 66.33 ... glucose (4.5 gÆL )1) , 10 % fetal bovine serum, penicillin (10 0 UÆmL )1) and streptomycin (10 0 lgÆmL )1) Cells were seeded into 35 mm Petri dishes, at a density of 7.5 10 5 cells per dish Twenty-four ... was performed on frozen tissue: sonication in 0 .1 M HCl, followed by boiling for 10 Tissue homogenates were buffered to pH 7.4 with M Tris pH 7.4, at a final concentration of 25 mg tissueÆmL )1 and...
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mass spectrometry of proteins and peptides
... 34–59 64–85 614 .6 /16 39.7 2287.5 14 57.7 315 7.7 2432.8 Not isolated /16 39 2288/2288 14 57 /14 57 315 8/ 315 8 2433/2433 Fig Comparison of the C18 RP-HPLC chromatograms of the Asp-N digestion of rCP10 (Digest ... for details) Protein rCP10 Mutant CP10 rCP10Dimer GST Fusion Protein Mutant fusion Protein Calculated mass (Daltons) ESI mass (Daltons) 10 ,307.6 11 ,332.7 20, 614 .1 26 ,16 6.6 36,456 .1 37,4 81. 2 10 ,308 ... by rCP10 at 14 .1 (Fig 2A) The disulphide-linked homodimer of rCP10 eluted at 15 .1 followed by GST at 17 .5 (Fig 2A) The ratio of mutant protein to rCP10 was approx 1: 10, and this ratio did not vary...
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Báo cáo toán học: " Measurement of beta amyloid peptides in specific cells using a photo thin-film transistor" pot
... with Alzheimer disease-like pathology Proc Natl Acad Sci USA 19 97, 94 :13 287 -13 292 Nussbaum RL, Ellis CE: Alzheimer’s disease and Parkinson’s disease N Engl J Med 2003, 348 :13 56 -13 64 Hintersteiner ... 2007, 8 :10 111 2 13 Bonte FJ, Harris TS, Hynan LS, Bigio EH, White CL: Tc-99m HMPAO SPECT in the differential diagnosis of the dementias with histopathologic confirmation Clin Nucl Med 2006, 31: 376-378 ... 73:443-449 10 Sisodia SS, Price DL: Role of the beta-amyloid protein in Alzheimer’s disease FASEB J 19 95, 9:366-370 11 Lin H, Bhatia R Lal R: Amyloid β protein forms ion channels: implications for Alzheimer’s...
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Overview of User Interface Design docx
... test tasks is a critical issue no matter whether we develop a new system or buy an existing one – For some systems it is hard to find meaningful test tasks at all • For instance, this is the case ... something wrong on the screen • Missing functionality – If it is impossible to carry out the task, the system is not fit for use – We classify this as a usability defect – missing functionality • Ease-of-use ... cannot figure out how to it or doesn’t like the system, the system is not easy to use • This is another kind of usability problem – We classify them according to their severity to the user, for...
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Báo cáo y học: "Salivary gland derived peptides as a new class of anti-inflammatory agents: review of preclinical pharmacology of C-terminal peptides of SMR1 protein" pptx
... specific lung resistance; SMR1: submandibular rat -1; VCS -1: variable coding sequence -1; VPSP: ventricular peak systolic pressure Page 10 of 11 10 11 12 13 14 15 Competing interests DAG is a research ... 2009, 13 2 :17 5 -18 0 Page 11 of 11 54 John SM, Bao F, Chen Y, Mathison RD, Weaver LC: Effects of a novel tripeptide on neurological outcomes after spinal cord injury Neuroreport 2006, 17 :17 93 -17 96 ... Reviews 19 98, 9 :10 1 -10 6 49 Mathison RD, Davison JS, Befus AD: The tripeptide feG reduces perturbation of intestinal motility provoked by anaphylaxis Proc West Pharmacol Soc 20 01, 44 :15 7 -15 8 50...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25