lcm and qrt pcr confirm expression of atcdc48 pde120 and ms5 like in arabidopsis thaliana seed
Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt
Ngày tải lên :
30/03/2014, 01:20
... contents in leaves of Arabidopsis plants cultured with mM nitrate in air (F) Ornithine, citrulline and arginine contents in leaves of Arabidopsis plants cultured with mM ammonium in air (G) Ornithine, ... arginine levels The levels of ornithine, citrulline and arginine remained low, at between 0.01 and 0.04 lmolặg)1 fresh weight (Fig 3E) However, arginine accumulated up to 70-fold and 80-fold in ... composed of 33 exons coding for a protein of 165 kDa, containing a class II (purF)-type glutaminase domain and short regions for binding to FMN and iron sulfur center GLT is composed of 20 FEBS...
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báo cáo khoa học: " Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds" potx
Ngày tải lên :
11/08/2014, 11:21
... that the expression patterns Figure Expression profiles of candidate imprinted genes in Arabidopsis thaliana seed as determined by qRT- PCR 1A Expression of AtCDC48A, MS5- like and PDE120 increases ... Analysis of expression profiles of ATCDC48, PDE120 and MS5- like in LCM tissues of Arabidopsis thaliana seeds (4 dap) Results of RT -PCR performed on cDNA derived from LCM endosperm (ES), seed coat ... imprinting in Arabidopsis thaliana (Figures and 3) While ATCDC48 and PDE120 are subject to binary imprinted expression, MS5- like shows a preferential maternal expression pattern of imprinting...
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báo cáo khoa học: " The effect of experimental warming on leaf functional traits, leaf structure and leaf biochemistry in Arabidopsis thaliana" pdf
Ngày tải lên :
11/08/2014, 11:21
... obtain an Page of 10 integrative understanding of the responses of leaf growth to global warming, we examined the effects of simulated climatic warming on SOD and CAT activities, contents of MDA, ... carefully reviewing our manuscript and providing us with many valuable suggestions In addition, we would like to thank Prof Yu-Xi Hu and Prof Jin-Xing Lin for valuable discussions during the early ... planting Then they were sown in 400-cm3 plastic pots containing a 1:1 (v/v) mixture of vermiculite and peat (Kaiyin Company, Beijing, China) The plants were grown in growth chambers (RXZ-300B, Ningbo...
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báo cáo khoa học: " Comparative transcriptomic characterization of aluminum, sodium chloride, cadmium and copper rhizotoxicities in Arabidopsis thaliana" ppsx
Ngày tải lên :
12/08/2014, 03:20
... ADOF1 ATSERAT2;1 Ca- and calmodulin binding and related proteins Calmodulin related and of similar protein Calmodulin binding and of similar protein Ca-binding protein and of similar protein ... DNA or RNA binding hydrolase activity kinase activity nucleic acid binding nucleotide binding other binding other enzyme activity other molecular functions protein binding receptor binding or activity ... contained various Ca-binding proteins, including previously identified calmodulin -like proteins (CML37/39 and 38), which were inducible by various stimuli [29], suggesting that Ca-mediated signaling...
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Báo cáo y học: "At-TAX: a whole genome tiling array resource for developmental expression analysis and transcript identification in Arabidopsis thaliana" pps
Ngày tải lên :
14/08/2014, 20:22
... (bandwidth in steps of 25 between 50 and 150, signal threshold between and 13, minimum run in steps of 20 between 20 and 100, and maximum gap in steps of 20 between 40 and 100) As optimal setting ... pairs of neighboring genes, one instance of mSTAD was trained on 500 of these genic regions and hyper-parameters were tuned on another 500 genic regions We trained and tuned a second instance of ... Comparison of expression estimates on tiling and ATH1 array platforms Scatter plot of expression estimates in (a) roots and (b) inflorescences (c) Correlation between expression changes between roots and...
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Functional characterization of giant killer in flower development and meristem regulation in arabidopsis thaliana 1
Ngày tải lên :
14/09/2015, 08:46
... through binding assay and expression analysis The GIK protein contains an AThook DNA binding motif that is widely found in chromosomal proteins and that binds to nuclear matrix attachment regions of ... 1.3 Regulation of flower development in Arabidopsis thaliana ……………………….9 1.4 Patterning and differentiation of lateral organs in Arabidopsis thaliana 13 1.4.1 The patterning of lateral organs…………….……………………………13 ... targets of AG In the first part of my study, I demonstrate that GIANT KILLER (GIK) is a direct target of AG in the regulation of reproductive organs patterning and differentiation in Arabidopsis thaliana...
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Functional characterization of giant killer in flower development and meristem regulation in arabidopsis thaliana 2
Ngày tải lên :
14/09/2015, 08:46
... analyze for the expression of GIK following AG induction in developing flowers The transgenic line contains a gene coding for a fusion of the AG protein and the steroid binding domain of the rat glucocorticoid ... meristem and floral primordium in Arabidopsis thaliana L1, L2, and L3 layer of the meristem is shown CLV3 expression domain is highlighted in pink, and WUS expression domain is highlighted in green ... expression of REV and PHV at the abaxial site In kan1 kan2 double mutant, the confinement of REV and PHV expression to the adaxial domain is delayed In addition, the expression level of REV and...
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Báo cáo khoa học: Identification of a glycosphingolipid transfer protein GLTP1 in Arabidopsis thaliana ppt
Ngày tải lên :
23/03/2014, 07:20
... lysine in both AtGLTP1 (Lys200) and At3g21260, but an arginine in At1g21360 This makes the sugarbinding pocket of At1g21360 very rich in arginines Fourthly, the residue corresponding to Val209 in ... assessed during plant growth and development (Fig 6) In young seedlings carrying AtGLTP1::GUS, staining in roots was restricted to the tips (Fig 6A,B) In the roots of more mature plants, staining was ... style of 3-week-old plants, and (I) the receptacle and anther filaments of 6-week-old plants In (F), GUS staining appeared to be more intense in stomatal cells, and staining was most evident in distal...
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báo cáo khoa học: " Assessing the contribution of alternative splicing to proteome diversity in Arabidopsis thaliana using proteomics data" docx
Ngày tải lên :
11/08/2014, 11:20
... the relative expression levels between two or more AS isoforms Indeed, the effect of lowering of the expected number of events would only further increase if unequal expression of isoforms would ... participated in the design of the study and in drafting the manuscript RCHJvH conceived of the study, participated in its design and coordination and helped to draft the manuscript All authors read and ... preference of trypsin to cleave after K- and R-residues [15], only a fixed number of peptides can, at A B Proteins Gene structures p2 p1 p1 p4 Isoform Isoform p3 p1 p2 p4 Exon skipping p1 Isoform Isoform...
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báo cáo khoa học: " Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis thaliana" pdf
Ngày tải lên :
11/08/2014, 11:21
... water, and incubated on 0.7% agar-containing Murashige and Skoog (MS) medium supplemented with MS vitamins and 3% sucrose at 4°C in the dark for d to - 14 - induce vernalisation Subsequently, the seeds ... and their mutated genes were determined by PCR or HRM analysis and sequenced using specific primers (see Additional files and 2) The M3 seeds of the mutants were harvested and the phenotype of ... are indicated in lower case Overlapping sequences found in deletion sites are highlighted in bold Inserted sequence is highlighted in bold and Italic - 28 - Table Classification of mutations induced...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
Ngày tải lên :
25/10/2012, 11:10
... http://www.medsci.org Int J Med Sci 2011, we connected the N-terminus of globular adiponectin and the C- terminus of GLP-1-A through the linker, which could make the N-terminus of GLP-1-A and the C-terminus of ... nucleotide including 45 bases was used to connect the 3’ terminus of GLP-1-A gene and 5’ terminus of gAd gene The product of this nucleotide sequence was a glycine-rich short peptide including 15 amino ... fusion protein was cloned into the expression vector PET28a at Nhe I and HindIII sites Expression of N-terminal His-tagged gAd-GLP-1-A fusion protein and Western blot analysis Protein expression: ...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
Ngày tải lên :
17/03/2014, 11:20
... mgÆL)1 of wild-type laccase from the dikaryotic strain I-937 of P cinnabarinus and 145 mgÆL)1 from the derived monokaryotic strain ss3 of P cinnabarinus In order to improve the secretion of the ... transferred in standard reaction mixtures to determine the laccase activity with ABTS and syringaldazine Mycelial dry weight and pH (g.L −1) P cinnabarinus laccase gene expression in A niger (Eur ... against buffer A Characterization of the recombinant laccase Protein analysis Protein concentration was determined according to Lowry et al [23] with bovine serum albumin as standard Protein...
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Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx
Ngày tải lên :
30/03/2014, 11:20
... translocation of these proteins [26–28] The expression of GL and PTG, but not R6, in rat liver in vivo is insulin-dependent It declines during insulin deficiency and is restored by insulin treatment [22,40] ... changes in coupling mechanisms between phosphorylase-a and glycogen synthesis Using three independent methods involving either expression of the muscle isoform of glycogen phosphorylase, or expression ... synthesis and glucokinase activity was determined by overexpression of glucokinase with varying titres of recombinant adenovirus Although glycogen synthesis increased with titrated glucokinase expression, ...
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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
Ngày tải lên :
18/06/2014, 19:20
... DNR-sensitive U937 cells and DNR-insensitive KG1a and Kasumi-1 cells via the Page 13 of 15 intrinsic apoptosis pathway involving down-regulation of Bcl-2 protein, loss of MMP and activation of caspase-3, ... participated in its design and coordination DX contributed effort in designing, performing, and analyzing the results and conclusion All authors read and approved the final manuscript Competing interests ... cells Curcumin decreased Bcl-2 mRNA and protein levels and reduced MMP in both DNR-insensitive and -sensitive AML cell lines The mechanisms underlying curcumin-induced apoptosis were investigated...
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Báo cáo hóa học: "Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development" pdf
Ngày tải lên :
21/06/2014, 19:20
... is also interest in understanding the role of 14-3-3 proteins in the regulation of oogenesis and the cell cycle during oocyte maturation and in early development In addition, 14-3-3 proteins, by ... target proteins and alter cellular function by binding to and causing conformational changes in target proteins or modifying target protein interactions with other proteins Of particular interest, ... of proteins Seven mammalian isoforms of 14-3-3 are known (β, γ, ε, ζ, η, τ and σ) These proteins associate with many intracellular proteins involved in a variety of cellular processes including...
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Báo cáo y học: "Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue" doc
Ngày tải lên :
09/08/2014, 03:24
... arthritis showing positive staining for Ang-1 (b) Positive staining in osteoarthritis synovial tissue (c) Ang-1 staining is absent in normal synovial tissue (a), (b) and (c) Original magnification ... arthritis showing positive staining for Ang-2 (b) Positive staining for Ang-2 in osteoarthritis synovial tissue (c) Ang-2 staining is absent in normal synovial tissue (a), (b) and (c) Original magnification ... arthritis showing positive staining for Tie1 (b) Positive staining for Tie1 in osteoarthritis synovial tissue (c) Tie1 staining is absent in normal synovial tissue (a), (b) and (c) Original magnification...
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Báo cáo y học: "The proinflammatory cytokines IL-1β and TNF-α induce the expression of Synoviolin, an E3 ubiquitin ligase, in mouse synovial fibroblasts via the Erk1/2-ETS1 pathway" ppt
Ngày tải lên :
09/08/2014, 08:23
... moderate redness and swelling of ankle and wrist; grade 3, severe redness and swelling of entire paw, including digits; and grade 4, maximally inflamed limb with involvement of multiple joints Each ... upregulation of SYN expression is involved in the development of CIA in mice Induction of SYN expression by IL-1β and TNF-α in mouse synovial fibroblasts The production of proinflammatory cytokines by ... was induced by intradermal injection of DBA1 mice with 50 μl of emulsion containing 100 μg of collagen in CFA On day 21, the mice were boosted by intradermal injection with 100 μg of collagen in...
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Báo cáo khoa học: "Effect of neoadjuvant chemoradiation and postoperative radiotherapy on expression of heat shock protein 70 (HSP70) in head and neck vessels" potx
Ngày tải lên :
09/08/2014, 09:20
... coherence between high levels of HSP70 expression and the prevention of thickening of the intima layer of arteries and the media layer of vein from Funding The article processing charges are funded ... radiation in the intima layer of arteries and the media layer of arteries and veins Anticancer drugs by contrast increase the HSP70 expression in the media layer of the recipient vessels Comparing ... the expression of HSP70 [27-30,33,34] Our findings can be concluded in three major results: chemoradiation increases the HSP70 expression of the media layer in transplant and recipient veins...
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Báo cáo y học: "Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus" doc
Ngày tải lên :
09/08/2014, 14:22
... signaling components IRF9 and STAT1 in murine B-cell expression of TLR7 as well as in the IFN-I-mediated induction of TLR9 expression [32] We examined the mRNA expression levels of these TLRs in ... regulators of IFN and other proinflammatory cytokine signaling, including suppressor of cytokine signaling (SOCS1) and the Tyro-3, Axl, and Mer (TAM) receptors, have been shown to associate with, and ... bind to the IFN-I receptor (IFNAR), which is composed of two chains: IFNAR1 and IFNAR2 The IFNAR2 chain exists in both transmembrane and soluble isoforms and is critical for ligand binding and...
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