lc detection and quantitation by of ine mass spectrometry

... E1 region of known HPVs and should allow for detection of any of these types The second qPCR assay targets the coding region of the hypervariable loop V of the L1 gene of HPV6, 11, 16 and 18 These ... contained a mixture of × 105 copies of HPV6 L1, × 104 copies of HPV11 L1, × 103 copies of HPV16 L1 and × 102 copies of HPV18 L1 The assay was performed in the presence or absence of 100 ng of DG-75 ... 2) Both biopsies and fluids have been tested A subset of specimens (anogenital/skin and oral cancers) have been assessed by both standard assays and by the novel assay Detection of HPV in well...

Ngày tải lên: 12/08/2014, 04:20

... 10-80% of the base peak Low -mass sensitivity 68 50% of Mass 442 Mid -mass resolution and sensitivity 199 5-9% of Mass 198 Mid -mass resolution and isotope ratio 275 10-60% of the base peak Mid- to high-mass...

Ngày tải lên: 22/03/2014, 14:20

... Page of Figure Detection limits and amplification efficiency Detection limits and amplification efficiency of the multiplex RT-qPCR for detection and differentiation of clade 2.2.1p proper and ... development of a multiplex RTqPCR assay for detection and differentiation of Egyptian H5N1 HPAI viruses of clade 2.2.1 proper (p) and the emerging 2.2.1 lineage of viruses which escape standard vaccine-induced ... total of 50 HPAIV H5N1 isolates from Egypt obtained between 2006 and 2010 for which nucleotide sequences of the HA gene were available, were examined by the multiplex assay which assigned 33 of...

Ngày tải lên: 12/08/2014, 01:22

... analyzed using the SDS software provided by Applied Biosystems Results Design and evaluation of primers and probes Alignment of the 3'UTR sequences of 13 Indian strains of DENV and the 41 sequences ... the detection of DENV [number of negative specimens/(number of negative specimens + number of false positive specimens)] The detection limit of the assay was evaluated by both RNA copy numbers and ... 3) The detection limit of the assay varied for the four serotypes of DENV, the sensitivity of detection was highest for DENV-2 and lowest for DENV-4 (Fig Fig 2C–F, Table 3) Detection and Quantitation...

Ngày tải lên: 12/08/2014, 04:21

... Scandinavica 2009, 51:8 Five g of each sample of manure, soil and silage were placed in a separate bottle, 10 mL of sterile physiological saline were added and these samples were prepared by ... detecting of C chauvoei Detection threshold of the PCR method The detection thresholds for samples consisting of manure, soil, silage and biogas substrate before and after pasteurisation and digested ... cases of blackleg analysed by culture methods and identification by biochemical methods For PCR in combination with culture on FAA plates, 14 out of 25 (56%) of samples were above the detection...

Ngày tải lên: 12/08/2014, 18:22

... combinations of substrates that should allow synthesis of a diketide product and then analyzed as before to determine the nature and extent of occupancy of the KS, AT and ACP chain-extension domains of ... treatment of the diketide N-acetylcysteamine (NAC) derivative and the ACP-TE derivative with hydrazine to release the hydrazide product for analysis by MS SNAC, thioester of N-acetylcysteamine Finally, ... AT and ACP domains is probably determined (at least in part) by the relative stabilities of free methylmalonyl-CoA ester and the loaded forms of the domains, and thus by the concentrations of...

Ngày tải lên: 16/03/2014, 03:20

... MSE(b) −10 −20 −30 −40 The detection and parameters estimation of multicomponent LFM signal can be realized by the CPF The principle of detection and parameters estimation of single LFM signal is ... characteristics of LFM signal are determined by the chirp rate and initial frequency Remark For an LFM signal with finite length, the maximum value of its CPF is finite, and the result of Theorem is ... amplitude, initial frequency, and chirp rate, which can be illustrated by the definition of the CPF Remark The estimate of the chirp rate β can be obtained by finding the peak of CP(n, u) in (7) when...

Ngày tải lên: 21/06/2014, 22:20

... Gang, Analysis of curcuminoids by positive and negative electrospray ionization and tandem mass spectrometry, Rapid Commun Mass Spectrom 20 (2006) 1001–1012 [18] F Payton, P Sandusky, W.L Alworth, ... spectra of peaks and in positive and negative detections (a) Peak 1, positive, (b) peak 1, negative, (c) peak 2, positive, and (d) peak 2, negative [(Fig._3)TD$IG] Fig Mass spectra of peaks and with ... of keto form, and two phenolic hydrogens and one aliphatic hydroxyl hydrogen of enol form are exchangeable Mass spectra of curcumin tautomers showed apparent differences (Fig 3) In positive detection, ...

Ngày tải lên: 01/07/2014, 13:22

... curves have been constructed by plotting area against the standard concentrations of macrolides in the range of 0.001 ㎍/㎖ ~ ㎍/㎖ Limit of detection (LOD) and limit of quantitation (LOQ) were based ... and difficult derivatization procedure Our method has solved previous problems by application of liquid chromatorygraphy /mass spectrometry (LC/ MS) LC/ MS minimizes chromatographic separation and ... roxithromycin (B), tylosin (C) and tiamulin (D) Simultaneous Determination of Various Macrolides by Liquid Chromatography /Mass Spectrometry 107 Table Summarized mass spectra of the drugs used experiments...

Ngày tải lên: 07/08/2014, 15:20

... sensitive and specific method for the determination of roxithromycin in the broiler tissues by LC- MS has been established The limit of detection and limit of quantitation were ng/g and ng/g These ... of 0.999 Limit of quantitaion and limit of detection was ng/g and ng/g, respectively Precision and recovery are shown in Table and was satisfied with the guideline of NVRQS Re s idu e co n ce n ... Vetrinary Research and Quarantine Service (NVRQS) Limit of detection and limit of quantitation were based on the signal-to-noise ratio based on their areas The signal-to-noise ratio of was accepted...

Ngày tải lên: 07/08/2014, 17:22

... identities of 97∼ 98% and amino acid sequence identities of 96∼98% with Chinese IBVs (strain A2, SH and QXIBV) Discussion One of the major problems of IBV is the frequent emergence of new variants ... the second passage of each sample screened positive for the virus was also determined to be positive with RT-PCR amplification and a segment of 878-bp of the S1 gene was obtained (data not shown) ... typing of IBV Genotyping of IBV on the basis of the S1 gene sequence, particularly the HVR region of the S1 gene, is the most Nucleotide and amino acid sequence comparison The S1 gene of the...

Ngày tải lên: 07/08/2014, 23:22

... 42 to 51% of each mature protein sequence (Table 2) Proline and glutamine make up between 74 and 82% of the amino acid residues and determine the repetitive nature of this region Four of the Butte ... #3, 4, and 10 is indicated with an arrow deletion and 27 amino acid substitutions, one of which is a serine in place of a cysteine The protein encoded by contig #8 differs from that encoded by contig ... cysteine in proteins containing nine cysteines or residue substituted for cysteine in proteins containing seven cysteines is underlined determined by tBLASTn search of non-human, non-mouse ESTs,...

Ngày tải lên: 12/08/2014, 03:21

... morbidity and economic losses are significant An increase in the Brazilian ovine and caprine market has been observed over the last several years, leading to the emancipation of herds and Page of (page ... CB and EK participated in the planning of the project EK was the leader of the project ZL and CM collected the samples JA and RC, performed the PCR and phylogenetic analysis All authors read and ... fragment of the ORFV-MT05 and ORFV-NE1 major envelope protein gene (B2L) and comparison with Nucleotide Nucleotide sequence fragment of the ORFV-MT05 and ORFV-NE1 major envelope protein gene (B2L) and...

Ngày tải lên: 12/08/2014, 04:21

... DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CIVIL AND ENVIRONMENTAL ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2012 DECLARATION I hereby declare that the thesis is my original work and it ... batch adsorption and filtration of individual Hg(II) and HA solutions; sequential adsorption and filtration of individual Hg(II) and HA solutions; co-adsorption and co-filtration of solutions containing ... 6.3 Uptake of Cd(II) by CS/CA-SMP-TMPyP membrane in the presence of other cations ℃ ℃ ℃ vii LIST OF FIGURES Fig 2.1 Chemical structure of chitosan Fig 2.2 Comparison of morphology of the CS/CA...

Ngày tải lên: 09/09/2015, 17:52

... was taken to assess and optimize the detection sensitivity of pulsed laser with narrowband and wideband lock-in detection on both ac-coupled and dc-coupled detection systems By developing an analytical ... phase offset and with (b) 43 o phase offset Pulsed-DReM with narrowband lock-in detection experimental setup Detection sensitivity of pulsed-TIVA and pulsed-DReM with narrowband lock-in detection ... induced phenomena of a biased ix Abstract _ metal line structure, the sensitivity of pulsed laser with narrowband and wideband lock-in detection, and its dependence...

Ngày tải lên: 11/09/2015, 09:58

... measurement of the intermediate stage, f i (t ) , over the pulse period [93] Generally there are two types of lock-in detection, namely narrowband (NB) lock-in detection and wideband (WB) lock-in detection ... in Fig 5.9(a) and peaks at around kHz 5.2.2.3 DC-Coupled and AC-Coupled Comparison Fig 5.12 Narrowband and wideband lock-in detection magnitude output for dc-coupled and ac-coupled detection systems ... frequency with  LIA = ms and kHz pulsing frequency with  LIA = 500 μs (a) Line profile across AA‟ for kHz (b) Line profile across AA‟ for 200 Hz Fig 5.16 Line profiles across AA‟ of pulsed-TIVA images...

Ngày tải lên: 11/09/2015, 09:58

... FAULT DETECTION AND CORRECTION MODELING OF SOFTWARE SYSTEMS WU YANPING (B.S., USTC) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF INDUSTRIAL AND SYSTEMS ENGINEERING ... reliability of software has become the focus of basic requirement for computer system The reliability of software can get even worse with the increase of software complexity at the same time The software ... estimation of model parameters and prediction of future failures As a result, combined fault detection and correction modeling could present more practical models for software testing process, and it...

Ngày tải lên: 11/09/2015, 16:06

... ADAPTIVE FAULT DETECTION AND CONDITION MONITORING OF INDUCTION MOTOR LU WENJING (B.ENG NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING ... determined factors in the signal, reduce the randomness of signal Since entropy is a measure of the degree of uncertainty [27], signals with more determined information are less random and have ... feature extraction strategy and quantification method will reduce the human effort and surely improve the reliability and convenience of online fault detection Hence, the candidate proposes two techniques...

Ngày tải lên: 26/09/2015, 10:59

... Ta and Tb if the mass difference of the tail vertex of Ta and the head vertex of Tb is larger than the mass of smallest amino acid, and smaller than the max mass of i amino acids Special case of ... spectra data generated by mass spectrometry (MS) or tandem mass spectrometry (MS/MS) The advance in tandem mass spectrometry (MS/MS) technology has made highthroughput mass spectra generation possible ... features The advantage of these set of algorithms is accurate identification of peptides and PTMs, and show the power of combination of tags, SOM and MPRQ techniques for peptide and PTM identifications...

Ngày tải lên: 12/09/2015, 08:18

... cAMP Analogs and Amide H/2H Exchange Mass Spectrometry TANUSHREE BISHNOI A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE ... Analogs of cAMP; Rp-cAMPS and Sp-cAMPS Gel-Filtration Profile for PKA RIα91-244 with SDS-PAGE gel of purified sample(inlay) Figure 2-2 Gel Filtration profile of PKA-C with SDS-PAGE gel of purified ... biological effects of cAMP in mammalian cells are mediated through the two ubiquitous isoforms of the regulatory (R) subunit of PKA, types I and II (9) The regulatory subunits of these isoforms are further...

Ngày tải lên: 16/10/2015, 15:36