latent dirichlet allocation methods

Ngày tải lên: 07/03/2014, 22:20

... section 2 they will be in breach of the law and liable for a fine of $25 000. Safe carrying methods Carrying heavy objects can put a strain on your back, arms, shoulders and knees because ... that are too heavy for you to lift or carry. In these cases, you may need to use some of these methods: • Work with someone to carry the load. • Use a trolley. • Use a forklift or trolley jack...

Ngày tải lên: 21/08/2012, 17:56

... (FDMA) 1. A cellular system uses the FDMA, with a spectrum allocation of 12,5 MHz in each direction, a guard band of 10 kHz of each end of the allocation spectrum and a width of 30 kHz for each channel. ... - MOB Mobile Internet and Surrounding 1/12 Baey, Fladenmuller – Subject 5 MOB Subject 5 –Access methods Static and dynamic access techniques 1. Static access techniques 1.1 Space-division multiple-access...

Ngày tải lên: 17/09/2012, 09:13

... Biochem. Mol. Biol. 27, 5792. Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... separation between mucins Isolation of Large Gel-Forming Mucins 3 3 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... analyses. 3.6. Analysis of Mucins Methods for the detection and analysis of mucins are dealt with in other chapters in this volume. However, three principally different methods are available: solution assays...

Ngày tải lên: 23/09/2012, 19:25

... nonsulfated mucins remain unstained. Histological Methods for Detection of Mucin 29 29 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... tract. Histological Methods for Detection of Mucin 39 3. Alternatively, prepared adhesive slides such as Superfrost Plus ® /(Menzel-Gläser, Braunschweig, Germany) may be purchased. 4. There are many other methods ... the demonstration of mucin was mucicarmine (4), but this stain has now been largely supplanted by methods based on more strictly histochemical approaches that utilize a specific chemical reaction...

Ngày tải lên: 23/09/2012, 19:25

... Mucins can be detected by chemical methods within acrylamide Detection and Quantitation of Mucins 45 45 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited ... mucins, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: ... (6). References 1. Walsh, M. D., Jass, J. R. (2000) Histologically-based methods for detection of mucin, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 2....

Ngày tải lên: 23/09/2012, 19:25

... wrapping them in a 1–cm 2 sand- GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... mucous layer existed over the gastric mucosal surface (13,14). However, a variety of different methods (discussed subsequently) have now demonstrated unequivocally that a thick (100–200 µm), ... consisting primarily of a fibrin gel and necrotic cells with some mucin stain- ing (15). Original methods for measuring adherent mucous thickness in situ were based on (1) differences in refractive...

Ngày tải lên: 23/09/2012, 19:25

... PhosphorImager as au/A 260nm . Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... is isolated according to the manufacturer. Measure the following components: 68 Dekker et al. 3. Methods 3.1. General Assays for Quantitation of Mucin ( see Notes 1–3) Each of these six detailed...

Ngày tải lên: 23/09/2012, 19:25

... there are few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... Biotechnol. 5, 171–176. 10. Thornton, D. J., Howard, M., Devine, P. L. and Sheehan, J. K. (1995) Methods for separa- tion and deglycosylation of mucin subunits. Anal. Biochem. 227, 162–167. Separation ... monomers generated after reduction (M r = 2–3 × 10 6 ) are amenable to conventional biochemical separation methods. We describe a separation protocol based on anion exchange chro- matography to fractionate...

Ngày tải lên: 23/09/2012, 19:26

... 5). Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolute ... 96 Sheehan and Thornton 12. Koller, T., Harford, A. G., Lee, Y. K., and Beer, M. (1969) New methods for the prepara- tion of nucleic acid molecules for electron microscopy. Micron. 1, 110–118. 13....

Ngày tải lên: 23/09/2012, 19:26

... code as defined in Fig. 1. Amino Acid Analysis of Mucins 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... Mobile phase B: 15% (v/v) methanol in water. 6. Mobile phase C: 90% (v/v) acetonitrile in water. 3. Methods 3.1. Sample Preparation and Hydrolysis 1. Add an aliquot of a solution of a sample of mucin...

Ngày tải lên: 23/09/2012, 19:26

... Sequencing Glycopeptides 121 121 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... Elsewhere we have published alternative HPLC methods for the separation of PTH-glycosylated amino acids on a modified Beckman LF3600 sequenator (12). These methods were developed for two reasons: ... with high molecular weight, heavily glycosylated domains such as those found in mucins. The two methods for determining the sites of glycosylation in proteins are mass spectrometry and Edman sequencing....

Ngày tải lên: 23/09/2012, 19:26

... R Trityl C Synthetic Peptides for Antimucin Antibodies 129 129 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... cannot be controlled or simply that short peptides do not adhere well to polystyrene plates. Several methods of peptide modification have been utilized to overcome these problems. One such procedure ... epitopes defined by antibodies reactive with antigens of known primary structure. Unlike most other methods of simultaneous peptide synthesis, this technique allows the concurrent synthesis of hundreds...

Ngày tải lên: 23/09/2012, 19:26

... replaced with an 88-kDa band Dimerization of Domains of Mucin 143 143 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... 0.5% Nonidet P-40. 9. Neuraminidase incubation buffer: 40 mM Tris-HCl, 4 mM CaCl 2 , pH 7.8. 3. Methods 3.1. Synthesis of Rat Muc2 Domain Construct (pRMC) Using Recombinant PCR Figure 1 shows ... establish- ing the dimerization capability of the isolated C-terminal domain of rat Muc2, we describe methods for examining the role of glycosylation in dimerization by manipulating the sys- tem with...

Ngày tải lên: 23/09/2012, 19:26

... (Kdn), respectively. Substituents of the hydroxyl groups present on From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... sialic acid modifi- cations has been attempted after release and purification by improving the methods to avoid any destruction, and is achieved either with low concentrated acid solutions (3) or ... (β-elimination). In this case, GalNAc is converted into N-acetylgalactosaminitol (GalNAc-ol). Different methods are available for the analysis of monosaccharides depending mainly on the amount of material...

Ngày tải lên: 23/09/2012, 19:26

... GalNAc-Ser/Thr, often found in mucins, is destroyed by this procedure. From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... glycoforms isolated from rheumatoid sera: A criti- cal evaluation of chromatographic methods. J. Immunol. Methods 213, 113–130. 16. Guile, G. R., Rudd, P. M., Wing, D. R., Prime, S. B., and Dwek, ... and fluorescence detectors. 5. GlycoSepH™ and GlycoSepC™ HPLC column (Oxford GlycoSciences). 3. Methods 3.1. Alkaline Borohydride Degradation ( β -Elimination) 1. Release O-linked chains by treatment...

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26