lactalbumin oleic acid complex and its cytotoxic activity

... protein (GAP) activity [20,21] and a highly promiscuous C-terminally encoded ADPribosylation activity towards small GTPases [21–23] Its function is dependent on interactions with 14-3-3 and factor ... at position 428 is necessary for both the ADP-ribosylation activity and the cytotoxic action of ExoS in vivo Result and discussion Acidic residues within the 14-3-3 binding domain of ExoS are ... 14-3-3 and ExoS is important for the ADP ribosylation activity of ExoS and even more intriguingly, appears to be independent of serinephosphorylation [15,26] The amino acid sequence between 419 and...

Ngày tải lên: 19/02/2014, 08:20

... spectra before and after modification in Figure 3c, except for a few peaks at 3430, 1630, and 433 cm-1 corresponding to water (moisture) and ZnO nanocrystal, the peaks at 2936.3 and 2872 cm -1 ... vibration [26], and the peaks at 1330 and 1560 cm-1 of the stretching vibration of C-N and bending vibration of N-H can be found In addition, the strong absorption peaks at 3428.6 and 1633.5 cm-1, ... nanodisks, and nanomultipods were calculated, which are determined to be 3.78, 5.94, and 3.71 μmol/m , respectively The effects of morphologies of ZnO nanocrystals on HRP immobilization and their activity...

Ngày tải lên: 21/06/2014, 02:20

... cytokine-stimulated expression of sPLA2 and MMP genes and secreted proteins is depicted in Figure In this proposed model, PIP-18 binds sPLA2 and inhibits its enzymatic activity, leading to reduced PGE2production ... show that PIP-18 significantly inhibits sPLA2-IIA enzymatic activity and downregulates sPLA2-IIA and MMPs (MMP-1, MMP-2, MMP-3, MMP-9) at both the transcript and the protein level in IL1-β-induced ... mediate IL-1β-induced expression and synthesis, particularly of MMP-3 and MMP-1, at the level of transcription involving p38 MAPK and AP-1, while LY315920 may exert its effect via mediation of different...

Ngày tải lên: 09/08/2014, 14:22

... play within the protein complex itself [11] TIN2 and TPP1 are critical to its assembly [65], and the ssDNAbinding protein POT1 serves as the effector of the complex in its role of maintaining ... of the telosome and with a diverse array of proteins and protein complexes that are involved in the cell cycle and in DNA repair and recombination, to maintain telomere structure and length [2,21-28] ... mutant TRF1 induces mitotic entry and apoptosis [40] The MRN complex, functioning together with ATM, is also important for regulating TRF1 activity [49] The MRN complex appears to be required for...

Ngày tải lên: 14/08/2014, 20:22

... oleic acid and linoleic acid on Jurkat cells Clin Nutr 23, 721–732 Fast J, Mossberg AK, Svanborg C & Linse S (2005) Stability of HAMLET – a kinetically trapped alphalactalbumin oleic acid complex ... Morozova-Roche LA Oleic acid complexes of a -lactalbumin fragments 57 58 59 60 61 (2009) Protein oligomerization induced by oleic acid at the solid–liquid interface – equine lysozyme cytotoxic complexes ... is its capacity to interact with membranes and lipid bilayers [10–14], as well as fatty acids [15] In particular, a complex formed by Ca2+-depleted a-LA in its partly folded state with oleic acid...

Ngày tải lên: 06/03/2014, 09:22

... substances and activity is distributed over the whole middle and downstream excluding the upstream of Andong Relative estrogenic activity of estrogenic substances in the Sewage treatment plants and ... EE2, E2, E1, and APE increases at Goryung and Gumho river (Table1) The estrogenic activity increased at every spot excluding Namji and Nam River in May 2002 Yeast Two-hybrid assay and ELISA method ... streams, water treatment plants, and sewage treatment plants one each from the upstream of Andong area to downstream of Busan area of Nakdong river basin Estrogenic activity and concentration of estrogenic...

Ngày tải lên: 05/09/2013, 08:40

... behaviour and a low unstable inhibitory activity, and for I137A a biphasic loss of activity (Fig and Table 3) The phenotype of these substitutions is therefore different from that of N139A, T146A and ... with an A leads to substrate behaviour and a low, stable inhibitory activity (Fig and Table 3) This W is located in the cleft between hF and s2A (Fig 5), and the presence of an aromatic side chain ... amino acid substitutions (N152H, K156T, Q321L and M356I) [16] The three-dimensional structure of PAI-1stab has been determined and reveals that the stabilizing amino acid substitutions N152H and...

Ngày tải lên: 20/02/2014, 11:20

... IV and V blades and b-strands (1–6) from the internal b-sheet of the b-sandwich domain The last four amino acids of the native sequence at the C-terminus and the His-tag protrude from the b-sandwich ... lactic and acetic acids can be converted to butyric acid by other bacteria occupying the colon related to Roseburia and Eubacterium [32,33] Butyric acid regulates the proliferation of cells and ... C-terminal domain has a b-sandwich architecture and is composed of two six-stranded antiparallel b-sheets In both b-sheets the sixth strand is located between strands and Although the antiparallel...

Ngày tải lên: 06/03/2014, 00:21

... relation to its ability to perturb mitosis and microtubule assembly We found that sanguinarine inhibited microtubule assembly both in vitro and in cells and that the antiproliferative activity of ... tubulin)1-anilinonaphthalene-8-sulfonic acid complex fluorescence Hydrophobic fluorescence probes such as 1-anilinonaphthalene-8-sulfonic acid (ANS), bis-ANS and prodan are routinely used to determine ligand-induced conformational ... sanguinarine for 20 h, and microtubules and chromosomes were visualized as described in Experimental procedures Microtubules in the absence (A) and presence of 0.5 lM (B) and lM (C) sanguinarine...

Ngày tải lên: 07/03/2014, 12:20

... various systems comprised 3899 and 4516 SPC molecules for the agonist and the antagonist in water, respectively, and 817 and 927 Me2SO molecules for the agonist and the antagonist, respectively, ... agonist analogue) and Glu82 MD simulations of MBP(74–85), and Ala81MBP(74–85) in water and Me2SO To further assess the structural origin of the difference in activity between the agonist and antagonist, ... [82] and (B) sequence alignment of the Vb chain TCR(79–83), MBP(74–85) and MBP(95–103) P5 and P8 are prominent, solvent-exposed TCR contact residues of the MBP(86–105) peptide, and P4, P6 and...

Ngày tải lên: 07/03/2014, 16:20

... the two subunits in calpains [18] Indeed, the two subunits of 80 kDa (domains I–IV) and 30 kDa (domains V–VI), contain altogether 10–11 putative EF-hands motifs in domains IV, VI and II [1,19,20], ... domains and EF-hand motifs Finally, it allows dissociation of two aspects in the protease behaviour toward its target, binding and cleavage, in relation to the presence of Ca2+ ions Materials and ... domains IV and VI, which have a CaM-like structure and are members of the penta-EF-hand family of proteins [1], could be the binding structures of interfaces with cytoskeleton components [17] and contribute...

Ngày tải lên: 07/03/2014, 21:20

... of factor X and its Gla-containing fragments to membranes containing 25 mol% PtdSer in the presence of 10 mM Ca2+ obtained by evaluation of association and dissociation phases (I) and equilibrium ... the signals from the intermediate complex AB and the final complex AB2 However, the contribution of the second binding step to the total response is about 15%, and therefore the evaluation of the ... response at full saturation, [AB]0 ¼ and [AB2]0 ¼ The total response signal is the sum of the initial response signal Ri plus the signals from the complexes AB and AB2 or AB* for the bivalent model...

Ngày tải lên: 08/03/2014, 23:20

... structural elements of this protein required for its biological activity and for its hypusine modification, and thereby to gain insights into its function In spite of the high sequence conservation ... for eIF5A activity being regulated by Lys47 acetylation Therefore, we substituted Lys47 with three different amino acids, i.e acidic, neutral and basic amino acids The fact that eIF5A activity ... was no cross-reactivity between antibodies to human and yeast eIF5A (compare lanes 17 and 18 in Fig 2C, and lanes and in Fig 2D) The yeast eIF5A and most of the heIF5A mutants were readily detectable...

Ngày tải lên: 16/03/2014, 06:20

... aromatic amino acid and X is a variable amino acid) typical of retroviral nucleocapsid proteins and the presence of an glycine ⁄ arginine rich region (RGG) box between the first and second Zn knuckles ... annealing proving that this activity was dependent on phosphorylated CNBP itself Our results show that CNBP phosphorylation does not change single-stranded nucleic acid binding activity Instead, it ... mobility controls: labelled Comp-CT alone and a completely annealed reaction Samples were taken at 0, 1, 3, 10, and 30 Single-stranded (ss) and double-stranded (ds) species are indicated (B) Bar...

Ngày tải lên: 16/03/2014, 12:20

... quadricauda, Chlorella sorokiniana and Chlamydomonas reinhardtii A single 6.5kDa band in Scenedesmus and two closely migrating and 9-kDa bands in Chlorella and Chlamydomonas could be detected ... Deriphat/PAGE of PSII complexes isolated from wild-type and IC7: A, PSII core ˆ PSII complex containing at least CP47, CP43, D1, D2 and cytochrome b-559; B, PSII subcore ˆ PSII core complex lacking ... anti-(D1-Mp) Ig and anti-(D1-Ct) Ig according to Materials and methods Nt1 ˆ fragment of D1 between R8 and K238; Nt2 ˆ fragment of D1 between R8 and R225; Ct1 ˆ fragment of D1 between K238 and A344;...

Ngày tải lên: 17/03/2014, 11:20

... ) and no peptide (*)] IN strand-transfer activity was measured as described previously [58] and in Experimental procedures The Km and Vmax values for each IN peptide ratio were calculated and ... dependence of INs overall activity (3Â-end processing and strand transfer) on its concentration was studied (Fig 4) In the presence of INS, the dependence of INs overall activity on its concentration ... to IN decreases its afnity for DNA; peptides that slightly increase the Km but, in addition, modify the Vmax and Kcat values; and peptides that bind IN, but have no effect on its activity Structural...

Ngày tải lên: 22/03/2014, 16:21

... analogues and other longer ligands are required to establish the mechanism of ligand binding and product formation Ascorbic acid inhibits the functional activity of HylP2 Ascorbic acid has previously ... hyaluronate lyase (HylP2) and have shown biochemically that ascorbic acid inhibits the activity of HylP2 We report the detailed crystal structures of native protein HylP2 and two of its complexes with an ... substrate Ascorbic acid binding Ascorbic acid (Fig 1A) inhibits the activity of HylP2 The structure of the complex of HylP2 with ascorbic acid shows that three molecules of ascorbic acid bind to HylP2...

Ngày tải lên: 23/03/2014, 04:21

... repeats and of EGF-A and EGF-B, and thus might additionally contribute to the pH-dependent ligand release [25,33–35] In the LA repeats, which consist of approximately 40 amino acids and are organized ... experiments involving LDLR and LDL, LpR and HDLp remain in complex at endosomal pH This remarkable stability of the receptor– ligand complex appeared to be accounted for by the ligand-binding domain ... new findings that the complex of LpR and HDLp is stable at endosomal pH and EDTA-resistant, both in contrast to the complex of LDLR and LDL This stability of the LpR– HDLp complex is proposed to...

Ngày tải lên: 23/03/2014, 07:20

... interaction with oleic acid Oleic acid itself can induce some cytotoxic effects [59–62], but its cytotoxicity is significantly lower than that of proteinaceous complexes (Figs and S1) These results ... of oleic acid and structural changes in the protein [28] Complexes of bovine a -lactalbumin with oleic acid have also been produced using column chromatography and were designated as bovine a -lactalbumin ... to their structural and cytotoxic properties Complexes of human a -lactalbumin with oleic acid were first described in the 1990s by Svanborg and coworkers, and named human a -lactalbumin made lethal...

Ngày tải lên: 30/03/2014, 01:20