labeling in vivo engraftment and viability controls and gfp labeled mscs intravenous infusions
Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione" pdf
Ngày tải lên :
12/08/2014, 18:20
... medium containing glutamine, penicillin and streptomycin After washing with ice-cold PBS, stimulated cells were lysed with reducing sample buffer and incubated for 10 minutes on ice Proteins were ... buthionine sulphoximine (BSO) on CSC-induced epithelial wound closure in NCI-H292 and in PBEC Effect of buthionine sulphoximine (BSO) on CSC-induced epithelial wound closure in NCI-H292 and in PBEC ... the serine/threonine kinase Akt leading to increased cell survival and tumorigenesis in human airway epithelial cells [39], and a study showing that nicotine induces cell proliferation in neoplastic...
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An evaluation of the effects of the stiffness of polycaprolactone on cell proliferation
Ngày tải lên :
28/09/2015, 13:28
... fluorescence labeling of specific adhesion molecules such as vinculin, paxillin and integrins 2.8 Formation of focal adhesion points Initial adhesions are formed between integrin receptors and the ... [DePasquale and Izzard, 1987; Izzard, 1988] and contain all of the components of initial adhesions as well as vinculin, paxillin, and phosphoproteins [Nobes and Hall, 1995] Focal complexes can continue ... evaluated via cell proliferation and viability studies conducted using Fluorescein Diacetate (FDA) / Propidium Iodide (PI) Staining, Actin Cytoskeleton and Focal Adhesion Staining, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenlytetrazolium...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
Ngày tải lên :
18/06/2014, 15:20
... concentration, the staining curve increased faster than at higher concentration, indicating a two-component binding interaction Indeed, using a root mean square minimization analysis and the Akaike ... increases Kd showing that both dendrimers are competing for same binding sites c) Equilibrium binding curve of Julo-3a-G1, Kd and Bmax, comparing CD4, CD8 T cells and NK cells using monocyte depleted ... analysis Eventually, intracellular staining of FoxP3 was done using Foxp3 Staining Buffer Set (eBioscience) following manufacturer's instructions CFSE dilution, NK cell amplification and cell cycle...
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báo cáo hóa học:" Effect of borax on immune cell proliferation and sister chromatid exchange in human chromosomes Malinee Pongsavee" docx
Ngày tải lên :
20/06/2014, 00:20
... cells in tissue culture provides a potential mechanism for increasing invasive mammary carcinoma [5] and induced colonic neoplasia in animal models [6] Borax is used as a food additive in some ... fetal calf serum (Invitrogen, Carlsbad, CA, USA) and penicillin-streptomycin antibiotic Phytohemagglutinin was added in RPMI medium as the mitogen One ml of μg/ml BrdU was added in each lymphocyte ... Phytohemagglutinin was added in RPMI medium as the mitogen and incubated for 72 hours After 72 hours incubation, a total of × 105 cells/well from each lymphocyte culture were selected in the 24-well...
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báo cáo hóa học:" The effect of newer anti-rheumatic drugs on osteogenic cell proliferation: an in-vitro study" doc
Ngày tải lên :
20/06/2014, 01:20
... Scotland) to remove cells remaining in the intra-trabecular spaces and plated at fragment per well in a 24-well plate containing ml of control medium (α-MEM containing 1% Gentamicin v/v and 10% ... increasing the action of the first and negating that of the second That drugs can interact is not unexpected Systematically investigating such interactions in vitro is slowly becoming a routine ... decreased cell number (Table 3) In addition, Etoricoxib and Dexamethasone in combination had a constant negative interaction effect (Table and Figure 2) In this case, administering Etoricoxib negated...
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báo cáo hóa học:" The Photodynamic Effect of Different Size ZnO Nanoparticles on Cancer Cell Proliferation In Vitro" docx
Ngày tải lên :
21/06/2014, 18:20
... 3.12, 6.25, 12.5, 25 and 50 lg/mL, respectively Initially, 104 cells were seeded into each well containing 200 lL cell culture mediums in 96-well plate and incubated for 24 h and then added the ... maintained in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (Sigma, USA), 100 U/ml penicillin (Sigma, USA) and 100 lg/ml streptomycin (Sigma, USA) and grown at 37°C in ... monitoring of dynamic changes induced by cell-chemical interaction [38–41] Since the relevant test is labeling free, the RT-CES assay allows real-time, automatically and continually monitoring cellular...
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Báo cáo y học: "The effect of substance P on asthmatic rat airway smooth muscle cell proliferation, migration, and cytoplasmic calcium concentration in vitro" pdf
Ngày tải lên :
11/08/2014, 03:20
... neurokinin (NK)-1 and NK-2 receptors, leading to potent effects on airway smooth muscle tone and secretions, bronchial circulation, and inflammatory and immune cells [10] Tachykinin levels were increased ... mg/ml aluminum hydroxide (Sigma-Aldrich, Beijing, China) in ml PBS and intraperitoneal (ip) injection of ml heat-killed Bordetella pertussis bacteria (6 × 109/ml, Beijing, China) were administered ... measurement was performed using rat IgE ELISA quantification kit (R&D ELISA KIT, DoBio Biotech, Shanghai, China) Hematoxylin and eosin staining Routine histological staining methods were applied...
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Báo cáo y học: "The effect of substance P on asthmatic rat airway smooth muscle cell proliferation, migration, and cytoplasmic calcium concentration in vitro" potx
Ngày tải lên :
11/08/2014, 06:23
... neurokinin (NK)-1 and NK-2 receptors, leading to potent effects on airway smooth muscle tone and secretions, bronchial circulation, and inflammatory and immune cells [10] Tachykinin levels were increased ... mg/ml aluminum hydroxide (Sigma-Aldrich, Beijing, China) in ml PBS and intraperitoneal (ip) injection of ml heat-killed Bordetella pertussis bacteria (6 × 109/ml, Beijing, China) were administered ... measurement was performed using rat IgE ELISA quantification kit (R&D ELISA KIT, DoBio Biotech, Shanghai, China) Hematoxylin and eosin staining Routine histological staining methods were applied...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
Ngày tải lên :
12/08/2014, 11:23
... following a 5-minute incubation at 37°C with 0.05% trypsin/ethylenediaminetetraacetic acid (EDTA) (Gibco) and replated MSCs of C57BL/6 origin were kindly provided by Darwin J Prockop and Catherine ... MSCs, we addressed the effect of MSCs on in vitro and in vivo Tcell proliferation and on CIA in this study In addition, we investigated the role of IFN-g by using MSCs isolated from IFN-g receptor ... (axillary, inguinal, and mesenteric) and spleens were harvested Single-cell suspensions were obtained as described above and were incubated for 15 minutes with the Fc receptor-blocking antibodies...
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Báo cáo y học: " Effect of hypoxia and Beraprost sodium on human pulmonary arterial smooth muscle cell proliferation: the role of p27kip1" ppsx
Ngày tải lên :
12/08/2014, 15:21
... then counted and mRNA and protein stability was examined per 50,000 cells incubated with Act D and CHX using RT-PCR and Western blotting, respectively Each assay was repeated in independent experiments ... harvested with trypsinEDTA and fixed using 70% ethanol The ethanol was removed and the cells were incubated in PBS containing RNase (172 k units/ml) at 37°C for 30 minutes, stained with propidium ... cells were incubated in 75-cm2 tissue culture flasks (Corning, NY, U.S.) in a cell-culture incubator (37°C, 5% CO2, and 95% air) and used at the seventh passage after trypsinization in all experiments...
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Báo cáo y học: "Cord blood versus age 5 mononuclear cell proliferation on IgE and asthma" ppt
Ngày tải lên :
13/08/2014, 13:22
... antigen-induced CBMC T helper (Th) cytokine production, and compared their levels with the likelihood of later atopy For example, increased phytohemagglutinin (PHA)-induced interleukin (IL)-5 and ... centrifugation and plated in triplicate for mononuclear proliferation and in duplicate for cytokine assays Antigen-induced mononuclear cell proliferation and cytokine production were measured in cord ... biomarkers such as indoor antigen-specific proliferation and cytokine production and determine whether either was associated with IgE and symptoms related to atopy and asthma in an inner city prospective...
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Control of Cell Proliferation and Growth byMyc Proteins
Ngày tải lên :
25/10/2013, 21:20
... TRRAP binds to a highly conserved domain in the amino-terminus of Myc proteins (MycboxII), and Myc controls acetylation of its target genes in a MycboxII-dependent manner Mutations in this domain ... completely clear and may involve direct interactions of both proteins Alternatively, ARF acts through inhibiting the E3-ligase function of HectH9/p500ARF-BP1, a major ARF binding protein in human cells ... proliferation, since Myc leads to an activation of E2F-dependent transcription and distinct activating E2F proteins act downstream of Myc in controlling cell proliferation and tumorigenesis (Baudino et...
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Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc
Ngày tải lên :
19/02/2014, 13:20
... caspases are induced in BJAB cells following the initiation of apoptosis, but KIPase activity remains constant In order to ascertain whether KIPase behaves in a proliferation dependent manner in cells ... and KIPase during lymphoid cell apoptosis BJAB cells (A) and Jurkat cells (C) were exposed to anti-FAS serum as described in Fig and the activity of caspases and KIPase determined using the in ... described in Materials and methods and the activity of caspases and KIPase determined over a 24 h time course using in vitro fluorogenic assays The maximum induction of activity, observed 24 h post induction,...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
Ngày tải lên :
21/02/2014, 01:21
... to a scintillation vial containing 3.5 mL of INSTA-GEL scintillation fluid (Packard instrument Co., Meriden, CT, USA), and radioactivity was determined by liquid scintillation counting using a ... (2001) Suppression of urokinase expression and Invasiveness by urinary trypsin inhibitor is mediated through inhibition of protein kinase C- and MEK/ERK/c-Jun – dependent signaling pathways J Biol ... maintained in a standard culture incubator with humidified air containing 5% CO2 at 37 °C In selected experiments, cells were serumstarved by incubation in a serum-free medium M199 containing antibiotics...
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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
Ngày tải lên :
07/03/2014, 00:20
... Polypyrimidine tract-binding protein promotes insulin secretory granule biogenesis Nat Cell Biol 6, 207–214 Kosinski PA, Laughlin J, Singh K & Covey LR (2003) A complex containing polypyrimidine tract-binding ... MR & Hardy S (2004) Polypyrimidine tract-binding protein is involved in vivo in repression of a composite internal ⁄ 3¢-terminal exon of the Xenopus alpha-tropomyosin Pre-mRNA J Biol Chem 279, ... severe defect in cell proliferation in vivo and in vivo (A) Quantitative real-time PCR analysis comparing the expression of differentiated markers in wild-type and Ptb) ⁄ ) ES cells and EBs from...
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Báo cáo khoa học: Rotenone inhibits mammalian cell proliferation by inhibiting microtubule assembly through tubulin binding Pallavi Srivastava and Dulal Panda ppt
Ngày tải lên :
07/03/2014, 05:20
... after binding to tubulin [26] The competition between rotenone and colchicine for tubulin binding was examined using the fluorescence of tubulin–colchicine complex Tubulin (7 lm) was first incubated ... rotenone on the ligand binding to tubulin (A) Rotenone inhibited the binding of colchicine to tubulin Tubulin (7 lM) was incubated without (s) or with lM (d), 10 lM (h), 20 lM (j) and 50 lM (n) rotenone ... v) fetal bovine serum, 1.5 gÆL)1 of sodium bicarbonate, and 1% antibiotic antimycotic solution containing streptomycin, amphotericin B, and penicillin Cells were maintained at 37 °C in a humidified...
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Báo cáo khoa học: Cartducin stimulates mesenchymal chondroprogenitor cell proliferation through both extracellular signal-regulated kinase and phosphatidylinositol 3-kinase⁄Akt pathways pot
Ngày tải lên :
16/03/2014, 13:20
... chondrocytes in vivo in an autocrine ⁄ paracrine fashion only The expression of cartducin starts in the sclerotome, which contains a chondrogenic cell lineage during mouse embryogenesis Recombinant ... Laboratories, Linz, Austria), mm l-glutamine, and penicillin ⁄ streptomycin at 37 °C in a humidified atmosphere containing 5% CO2 To investigate the effects of cartducin on the MAPK or PI3K ⁄ Akt signaling ... hibernation and insulin-resistant obesity More recently, we found that cartducin is strongly expressed in both the chondrogenic cell lineage in the sclerotome and proliferating chondrocytes in the...
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Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx
Ngày tải lên :
17/03/2014, 09:20
... proteins were detected in quiescent SMC (Fig 4), however, both the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, ... bacitracin and 20 lgÆmL)1 aprotinin] were loaded (without heating) onto a 10% polyacrylamide gel containing 0.5 mgÆmL)1 myelin basic protein Following electrophoresis, phosphorylation of myelin basic ... similar results labeling of the 140, 133, 116, 95, 82 and 73 kDa proteins, while the labeling of several proteins (66, 56 kDa) was unchanged The insensitivity of the 56 and 66 kDa bands to MIBG...
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Báo cáo khoa học: RNF13: an emerging RING finger ubiquitin ligase important in cell proliferation pptx
Ngày tải lên :
22/03/2014, 17:20
... substrate binding or to be involved in protein–protein interactions [7–9] The RING region is a C3H2C3 type and exerts E3 activity [5,6] Bioinformatics predictions and experimental evidence indicate ... staining of 18S and 28S ribosomal RNAs and immunoblotting of tubulin were used as equal loading controls [11] from multivescular endosomes to recycling endosomes and inner nuclear membrane in ... especially in the liver and brain [6] More interestingly, RNF13 expression is upregulated by tenascin and myostatin [11,12] Myostatin is a member of the transforming growth factor-b superfamily and...
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Báo cáo khóa học: Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis doc
Ngày tải lên :
30/03/2014, 13:20
... the interactions among the S4, S5 and 16S rRNAs that maintain the open form are destroyed in ram mutants, which are inclined toward the closed form and result in an error-prone phenotype [19] In ... in the A-site interacted with H69 in the 23S rRNA and the S12 protein Conformational changes in the S12 protein and the 16S rRNA should be required to initiate GTP hydrolysis According to the K42T ... RNA– protein interactions in 30S ribosomal subunit: folding and function of 16S rRNA Science 244, 783–790 11 Allen, P.K & Noller, H.F (1989) Mutations in ribosomal proteins S4 and S12 in uence...
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