labeling hybridization and data acquisition

... probe saturation Resulting jpeg and data files were analyzed via mAdb Gateway Analysis tool [40] Data were further analyzed using Cluster and TreeView software [12] and Partek Pro software (Partek) ... ligand (CD40L), and FLT-3 ligand, which are generally associated with the classical pathway of MP activation [2,3] Therefore, we considered the first cluster representative of the alternative and ... example, IL-4 and IL-13) and the red bar indicates type I cytokines (such as IFN-γ, CD40L and FLT-3L) A smaller third class including IL-10 is also shown (green bar) TNF-α (purple bar), TNF-β and GM-CSF...

Ngày tải lên: 14/08/2014, 14:21

... cellulose and with cellobiose (the main product of cellulolysis), and with a mixture of glucose and cellobiose Cell extracts and extracellular media were analysed by 2D NMR spectroscopy and by TLC ... the incubation were taken at 1, and h, and extracellular and intracellular media were separated by centrifugation and analysed in parallel by 1H NMR spectroscopy and by TLC Under these conditions, ... separated by centrifugation and analysed, in parallel, by 1H NMR spectroscopy and by TLC Consumption of the substrates and production of the final metabolites (succinate and acetate) were quantified...

Ngày tải lên: 07/03/2014, 17:20

... animals were killed by cervical dislocation, and tibialis anterior, gastrocnemius and soleus muscles were carefully dissected and kept on ice In fasted animals and the respective controls, one gastrocnemius ... the soleus (1.5-fold) and in IMF mitochondria in the tibialis anterior and in the gastrocnemius than in the soleus (2.0-fold and 1.9-fold, respectively) In the gastrocnemius and soleus muscles, ... protein levels and (B) COX protein levels in mouse gastrocnemius SS (empty columns) and IMF (shaded columns) mitochondria (20 lg) The methods for the detection and quantitation of UCP3 and COX are...

Ngày tải lên: 24/03/2014, 04:21

... experiments, analyzed and interpreted the data, prepared the figures and table, wrote the manuscript and helped in designing the experiments CL and MTT performed data analysis and helped in writing ... chemotaxis, and cell adhesion) and proteolysis, and certain metabolic and signal pathways To validate our microarray findings at different phase of AA, we performed q-PCR on a set of randomly selected ... transduction and signaling pathways Phosphorylation and dephosphorylation, Kinase activity and regulation, Signal transduction and regulation, G-protein-coupled receptor signaling pathway, Others Tumor and...

Ngày tải lên: 12/08/2014, 18:20

... new broad band centred at 465 nm appeared and increased in Fig The dependence of e (top) and k (bottom) on the molar ratio of Yb3+ to apo-Tf In the lower panel the data of k1, k2 and k over the ... 20,400, 21 000 and 22 000 M)1Æcm)1 for Lu3+, Sm3+ and Eu3+, respectively [34] Both UV and ICP-AES data suggested that two Yb3+ bind to apo-Tf in the specific iron binding site and two tyrosines ... lM apo-Tf in mM NaHCO3 and 10 mM Hepes buffer at pH 7.4 and 310 K) after addition of various amounts of molar ratios of Yb3+ (as YbCl3) The two absorption bands at 242 and 295 nm are indicative...

Ngày tải lên: 08/03/2014, 09:20

... ðMr;analyte =Mr;ligand Þnð0:4ÞRimmobilized ððdn=dcanalyte Þ=ðdn=dcligand ÞÞ ð2Þ where (dn ⁄ dcanalyte) and (dn ⁄ dcligand) are the refractive index increment for the analyte and ligand, respectively ... 2006 FEBS 515 Grp94–ligand interactions M Ying and T Flatmark and a negative DRU due to the ligand-induced conformational change, no exact value for the binding affinity and the stoichiometry of ... Rmax ðtheoreticalÞ ¼ ðMr;analyte =Mr;ligand ÞnðAÞRimmobilized ð1Þ where Mr,analyte and Mr,ligand are the relative molecular mass of the analyte and ligand, respectively; n is the number of analyte-binding...

Ngày tải lên: 30/03/2014, 11:20

... contributions RS, NA and SN contributed to the concept and design of the study, and to the manuscript writing SN, RS and AA performed isolated of monocytes and culture of macrophages RS and AA performed ... analysis (data not shown) prior to culture Macrophage stimulation After days, the media and non-adhered cells were removed and replaced with complete media (DMEM, 10% FBS, gentamicin) and incubated ... an effect on MMP, TIMP, TNF-a and IL-6 levels Finally, the third tier sought to contrast dose and compare one drug with another Data were expressed as mean ± SEM and compared using a twotailed...

Ngày tải lên: 11/08/2014, 03:20

... contributions RS, NA and SN contributed to the concept and design of the study, and to the manuscript writing SN, RS and AA performed isolated of monocytes and culture of macrophages RS and AA performed ... analysis (data not shown) prior to culture Macrophage stimulation After days, the media and non-adhered cells were removed and replaced with complete media (DMEM, 10% FBS, gentamicin) and incubated ... an effect on MMP, TIMP, TNF-a and IL-6 levels Finally, the third tier sought to contrast dose and compare one drug with another Data were expressed as mean ± SEM and compared using a twotailed...

Ngày tải lên: 11/08/2014, 06:22

... Andong Daegu Busan Andong Waegwan Gangjung Daegu Gumho Riv Goryung Brd Hwang Riv Jeokpo Brd Namji RESULTS AND DISCUSSION Nam Riv Fig shows the sampling points along of Nakdong River in May, and ... excluding Namji and Nam River in May 2002 Yeast Two-hybrid assay and ELISA method for Nakdong River water revealed that estrogenic substances and activity is distributed over the whole middle and downstream ... E2, E1, and APE concentrations for the secondary treatment water and for ozone treatment water in the sewage treatment plants than for the inflow water (Figure3 and Table 2) In domestic and industrial...

Ngày tải lên: 05/09/2013, 08:40

... data cleaning, and data analysis for the DRD/IFPRI Rural Finance Study We thank Karid Chirwa, Tyme Fatch, Swalley Lamba, Samson Manda, and Franklin Simtowe, who provided invaluable research and ... the Data The information collected in the survey included data on household demographics, land tenure, agricultural production, and livestock ownership; asset ownership and transactions; food and ... Therefore we present data on households’ ownership of Further details on the survey and the data collection methodology are reported in Diagne, Zeller, and Mataya (1996) and Simtowe and Diagne (1998)...

Ngày tải lên: 16/02/2014, 03:20

... apply the Scrutton and Utter equation [40] to the inhibition data Data analyses Data were analysed by means of sigmaplot 2001 (SPSS Science Software Gmbh, Erkrath, Germany) Data for the characterization ... equation and the apparent KM and Vmax values were derived with their standard errors The competitive and noncompetitive Ki values were derived from the equations KMi ¼ KM (1 + [I] ⁄ Ki) and ⁄ VmaxI ... parameters of the Scrutton and Utter equation applied to data obtained with bilirubin and nicotinic acid are listed in Table As shown in Table 1, section C, these data are quite similar to those...

Ngày tải lên: 20/02/2014, 01:20

... to make them manifest, and the mentality that could conceive both, a mentality convinced by the correctness and naturalness of such abstract ideas as the rights of man and the legitimacy of democracy ... Italy, Nazi Germany, and Hussein's Iraq are some of Etlin's examples One legacy from revolutionary space is the concept of size and enormity Iraq's "Victory Arch" is two -and- a-half times larger ... ideals of unity, solidarity, and amity to which the Union is dedicated." The building itself is not too grand in size to impose a visitor, yet his subtle use of pilasters and a characteristic three-arch...

Ngày tải lên: 21/03/2014, 22:51

... Middle: bands of phosphorylated MBP by TSSK3 mutants were excised from gel and their radioactivity was measured by scintillation counting Data are representative of three independent experiments and ... rapid and strong activation of PKB (also known as c-Akt) [28] and this is mediated by phosphatidylinositol kinase (PtdIns3K) and PDK1 Thus A14 cells were transfected with HA-tagged TSSK3 and treated ... of standard phosphoamino acids are indicated (D) bands of peptides used in (B, C) in TSSK3 kinase assay, were excised from gel and their radioactivity was measured by scintillation counting Data...

Ngày tải lên: 23/03/2014, 11:20

... (Davidov and Rappoport, 2006; Widdows and Dorow, 2002) and meronymy (Berland and Charniak, 1999; Girju et al., 2006) Since named entities are very important in NLP, many studies define and discover ... 2004), meronymy (Berland and Charniak, 1999; Girju et al., 2006), synonymy (Widdows and Dorow, 2002; Davidov and Rappoport, 2006), and verb strength + verb happensbefore (Chklovski and Pantel, 2004) ... develop its own relationship definitions and dataset, like (Nastase and Szpakowicz, 2003), thus introducing a possible bias; or it can accept the definition and dataset prepared by another work, like...

Ngày tải lên: 23/03/2014, 17:20

... grammars G1 and G2, whose probabilities axe P1 and P2 respectively, is represented as follows: P(A =~ a) = ~IPI(A =~ c~) +,~P2(A =~ a) < ~ j < l ( j = l , 2) and ~ , + ~ = (4) where A E V and a E ... Zast(sig.(b))), where the functions cont, f u n c and sign take a bun~etsu as their argument and return its content word sequence, its function word sequence and its punctuation respectively In addition, ... to calculate the average cross entropy for all word-class relations and select the best one So we abandoned the best solution and adopted a greedy algorithm as shown in Figure in the descending...

Ngày tải lên: 31/03/2014, 04:20

... after being illuminated with light (365 nm wavelength; right) Lanes and 4, DCA only; lanes and 5, DCA and EDTA; lanes and 6, DCA and MAB Sizes of molecular-mass-marker proteins are shown at the ... exogenous enzyme, and that dansyl fluorescence was observed only in histones H2B and H3 Consistent with these results, nucleosomal histones H2B and H3, but not histones H2A and H4, are substrates ... three times by centrifugation and resuspension in labeling buffer Purification of histones Nuclei, before or after DCA -labeling, were washed three times in 0.14 M NaCl and 50 mM NaHCO3 by centrifugation...

Ngày tải lên: 31/03/2014, 07:20

... attenuation coefficient of the fiber, and Ep, Eprobe and Eidler are the electric field of the pumping, probe and idler waves γ is the nonlinear coefficient, and is obtained by 1) γ ≡ π fp n2 c ... should be minimized; and (c) states of polarization of the pump and signals must coincide As has already been argued in the literature, 6), 7) in order to broaden the conversion bandwidth, consideration ... dBm), and signal power was mW (0 dBm) From Figure it can be seen that as the length of the HNL-DSF is reduced, the bandwidth broadens, reaching 23.3 nm HWHM at a length of 200 m the greatest bandwidth...

Ngày tải lên: 16/07/2014, 08:55

... treatment of adipocytes with peptidoglycan and LPS, ligands for TLR2 and TLR4, could alter the expression of SLPI Both peptidoglycan and LPS (Figures 3A and 3B) upregulated expression of SLPI (P ... high fat diet, visceral (mesenteric and subcutaneous) vs subcutaneous depot and in stromal vascular cells vs adipocytes and whole adipose tissue (Figures 2A, B and 2C) Regulation of SLPI expression ... kit has an assay sensitivity of < pg/ml and an inter assay and intra assay variation of < 10% Statistical analyses All data were checked for normality and then analyzed using the GLM model analysis...

Ngày tải lên: 11/08/2014, 03:20

... treatment of adipocytes with peptidoglycan and LPS, ligands for TLR2 and TLR4, could alter the expression of SLPI Both peptidoglycan and LPS (Figures 3A and 3B) upregulated expression of SLPI (P ... high fat diet, visceral (mesenteric and subcutaneous) vs subcutaneous depot and in stromal vascular cells vs adipocytes and whole adipose tissue (Figures 2A, B and 2C) Regulation of SLPI expression ... kit has an assay sensitivity of < pg/ml and an inter assay and intra assay variation of < 10% Statistical analyses All data were checked for normality and then analyzed using the GLM model analysis...

Ngày tải lên: 11/08/2014, 06:22

... strains and from IBV live vaccines So frequent monitoring is highly essential to track the emergence of new variants and is mandatory to develop efficient vaccination strategies to control and prevent ... contributions VNV and JG conceived the study AA planned the experimental design, AA and CU carried out cloning and sequencing AA drafted the manuscript All authors critically reviewed and approved ... of IBV-Beaudette which is available in Coronavirus Database (CoVDB) [16] The nucleotide and the amino acid identity of Ark DPI with other IBVs and coronaviruses are listed in Tables 1, 2, The whole...

Ngày tải lên: 12/08/2014, 04:21