la and programmed cell death

... glioblastoma cell death A Lena et al Because of their multitask nature, ANTs are involved in several aspects of cell metabolism, as well as in cell survival ⁄ death processes, and have been related ... particular, ANT1 and ANT3 are considered to play a pro-apoptotic role [19,24], whereas ANT2 down-regulation produces a strong reduction in breast cancer cell viability and chemosensitizes HeLa cells ... in Scrambleand ANT1 and ANT2 siRNA-transfected cells; the 33 kDa band corresponds to ANT proteins, the 43 kDa band corresponds to actin (E) Western blot analysis of FLAGANT1 and FLAG-ANT2 recombinant...

Ngày tải lên: 29/03/2014, 09:20

... the understanding both of cellular and molecular mechanisms of cell demise, as well as of mechanisms of degenerative diseases, the confusion between apoptosis and programmed cell death has somewhat ... irradiation led to up to 30% cell death in MCF-7 cells without any signs of apoptosis In this case, cell death was accompanied by the formation of acidic vesicular organelles and lamellar structures, which ... with death domain (FADD) induced caspaseindependent cell death No DNA fragmentation was observed and dying cells showed neither condensation nor fragmentation of cells and nuclei, but the cells and...

Ngày tải lên: 07/03/2014, 15:20

... defence regulatory function 4668 Fig Role of EDS1 ⁄ PAD4 ⁄ SAG101 and BON1 ⁄ BAP1 + in cell death and plant pathogen resistance Whereas EDS1, PAD4 and SAG101 are positive regulators of cell death, ... JA and ethylene must be involved in the regulation of cell death in cet plants [93,94] JA action on mitochondria links oxidative damage to cell death Mitochondria play an active role in cell death ... cell death and increased disease resistance Plant Cell 13, 2225–2240 Yang H, Yang S, Li Y & Hua J (2007) The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death Plant...

Ngày tải lên: 16/03/2014, 02:20

... compilation ª 2009 FEBS 63 Blebbing in programmed cell death 10 11 12 13 14 15 16 17 18 19 20 21 22 23 64 M Bovellan et al dial germ cells: a role for myosin contraction and cytoplasmic flow Dev Cell ... apoptotic cell death resemble those of ‘healthy’ cells Growth and retraction FEBS Journal 277 (2010) 58–65 ª 2009 The Authors Journal compilation ª 2009 FEBS 61 Blebbing in programmed cell death ... [2] Filamin-deficient melanoma cells bleb constitutively because of decreased adhesion energy between the cortex and the cell membrane due to a lack of filamin [1,25,26] Consistent with this, filamin...

Ngày tải lên: 29/03/2014, 08:20

... specifically, PD-1, CTLA-4, Lag-3 and the prostaglandin receptor Ptger2 were all significantly up-regulated in antigen-specific CD8+ T cells from peptide (but not DNA) immunized mice, with the latter retaining ... antigen exposure and co-stimulation - the delicate balance between long-lived, readily expandable CD8+ T cells and short-lived T cells that are subject to exhaustion or other negative regulatory mechanisms, ... they upregulate PD-1 [53] During the next phase when the antigen exposure subsides, a minor subset of T cells down-regulate PD-1 and become memory cells, while the larger pool of effector cells extinguishes...

Ngày tải lên: 18/06/2014, 16:20

... regulates programmed necrosis and virus-induced inflammation Cell 2009, 137:1112-1123 Declercq W, Vanden Berghe T, Vandenabeele P: RIP kinases at the crossroads of cell death and survival Cell ... intracellular depletion of NAD+ and ATP, nuclear translocation of AIF and finally, death [14] Similar to mitochondria, the endoplasmic reticulum (ER) - as the main site for critical cellular functions ... W, Vandenabeele P: Death receptor-induced apoptotic and necrotic cell death: differential role of caspases and mitochondria Cell Death Differ 2001, 8:829-840 Annis MG, Yethon JA, Leber B, Andrews...

Ngày tải lên: 09/08/2014, 10:20

... flooded alveolar air spaces and increased microvascular and epithelial permeability due to neutrophil inflammation, damage to the alveolar capillary endothelium, and disruption of the alveolar epithelium ... growth and cytotoxicity of L pneumophila in A549 cells First, we verified the infection and intracellular growth of L pneumophila in A549 cells Intracellular growth of the virulent strain was ... Liautard JP, Dornand J: In vitro Brucella suis infection prevents the programmed cell death of human monocytic cells Infect Immun 2000, 68:342-351 Salvesen GS, Dixit VM: Caspases: intracellular signaling...

Ngày tải lên: 12/08/2014, 15:21

... Trehalase is present with the liquid silk in the silk glands, indicating that trehalase is produced by the silk gland cells and is localized to the cavity of ASGs [19,20] The silk gland and the ... strongly and equally expressed in ASGs and middle silk glands but not in posterior silk glands (Fig 3C, inset) During the fourth and fifth instar, GOD expression in ASGs was strong in IV2 and V0 larvae, ... of steroidtriggered programmed cell death in Drosophila Semin Cell Dev Biol 16, 237–243 Fahrbach SE, Nambu JR & Schwartz LM (2005) Programmed cell death in insect neuromuscular systems during...

Ngày tải lên: 15/03/2014, 00:20

... as playing a critical and early role in developmentally regulated PCD in the lace plant Background Programmed cell death in plants Programmed cell death (PCD) is the regulated death of a cell ... characteristic of PCD and has been noted previously during developmental cell death in the lace plant [10], in induced cell death in lace plant protoplasts [26], and also during induced cell death by osmotic ... premature programmed cell death and cytochrome c release Plant Cell 2001, 13:1803-1818 Selga T, Selga M, Pāvila V: Death of mitochondria during programmed cell death of leaf mesophyll cells Cell Biology...

Ngày tải lên: 11/08/2014, 11:21

... Types of cell death .9 1.2.1 Type I cell death – apoptosis 10 1.2.2 Type II cell death – autophagic cell death 16 1.2.3 Type III cell death – necrosis 21 1.3 Programmed ... Chromista and Heterokonta, are a diverse group of unicellular and multicellular protists comprising of heterotrophic and photosynthetic representatives, and are characterized by their flagella and ... 160 iv Summary Programmed cell death (PCD) is crucial for cellular growth and development in multicellular organisms Although distinct PCD features have been described for unicellular eukaryotes,...

Ngày tải lên: 16/10/2015, 15:35

... Sartorius, Gửttingen, Deutschland Mikrowelle HMT 702A Fa Bosch, Mỹnchen, Deutschland Laborkỹhlschrank Fa Siemens, Mỹnchen, Deutschland Brutschrank Fa Heraeus, Hanau, Deutschland DAKO TechMate 500 Fa ... Hilden, Deutschland 43 3.1.7 Primer miR21: MS00009079, Qiagen, Hilden, Deutschland RNU1A-1: MS00013986, Qiagen, Hilden, Deutschland RNU6B: MS00014000, Qiagen, Hilden, Deutschland 3.1.8 Zellkulturen ... erworben Ihre Lagerung erfolgte gemọò Herstellerangaben Xylol Fa Merck, Darmstadt, Deutschland Ethanol 98 % Fa Merck, Darmstadt, Deutschland Ethanol 90 % Fa Merck, Darmstadt, Deutschland Ethanol...

Ngày tải lên: 19/11/2015, 14:18

... (lanes 3, 4, and 5, respectively), 1, 5, and 10 mM EDTA (lanes 6, 7, and 8, respectively), and 0.1, 1, and mM ZnCl2 (lanes 9, 10, and 11, respectively) were assayed at 30 °C for 120 A standard reaction ... fraction; lanes and 8, post-mitochondrial fraction 1; lanes and 9, post-mitochondrial fraction 2; lanes and 10, cytosolic fraction 5382 coincubated with the isolated mitochondria at neutral pH, and ... apoptosis: How Pandora’s box opens Nat Rev Mol Cell Biol 2, 67–71 11 Ameisen JC (2002) On the origin, evolution, and nature of programmed cell death: a timeline of four billion years Cell Death Differ...

Ngày tải lên: 20/02/2014, 03:20

... ERK1 ⁄ and ⁄ or PKB signalling and, as a consequence, promote increased expression of BIM and BIM-dependent cell death in tumour cells Here we review recent advances in understanding BIM regulation ... increase BIM expression, and cell death was partially dependent upon BIM [42] Similar results in both colorectal cancer and melanoma cell lines have also been reported [43], and in all cases BIMEL ... mTOR, mLST8 and raptor) regulates cell growth via the effectors S6K1 and 4E-BP1, whilst BIM as a mediator of tumour cell death mTORC2 (composed of mTOR, mLST8 and rictor) phosphorylates PKB at...

Ngày tải lên: 16/03/2014, 00:20

... thrombin-treated platelet (lane 2; compare with control in lane 1); untreated platelets (lane 3); platelets treated for 24 h with zymosan (lane 4) or with OPZ (lane 5) externalization observed in platelets ... zymosan-treated cells (central panel) and OPZ-treated cells (right panel) (E) DIC (Nomarski) micrographs showing cell aggregates in zymosan-treated platelets (central panel) and OPZ-treated platelets ... hypothesized to play a role in production of so-called coated platelets, a sub-population of platelets observed upon dualagonist activation, e.g thrombin plus collagen, that express surface PS and can...

Ngày tải lên: 16/03/2014, 04:20

... Bacterial strains and plasmids E coli strains JM109 (endA1, thi, gyrA96, hsdR17 (rk), mk)), relA1, supE44, D(lac-proAB),[F¢, traD36, proAB, laclqzDM15]) and BMH 71-18 (thi, supE, D(lacproAB), [mutS::Tn10] ... strand, the resulting double-stranded plasmids were transformed into E coli BMH 71-18 The transformants were cultivated overnight in Luria broth with tetracycline Plasmid DNA was isolated and ... mutagenesis library, was diluted to an approximate cell density of · 103ÆmL)1 and plated (100 lLÆplate)1) on chitosan medium Plates were incubated at 37 °C and colonies were picked up after 48–72 h Revertant...

Ngày tải lên: 16/03/2014, 04:20

... transfected cells This confirms and expands the classification of estrogens, where planar estrogens such as E2 are classified as class I and all TPE-related estrogens are classified as class II estrogens ... Class I (planar) and Class II (non-planar) estrogens (Jordan et al 2001) A similar classification and conclusion has been proposed (Gust et al 2001), but the biological consequences of this classification ... mammals occurred a decade later Tritium-labeled hexestrol was found to accumulate in reproductive organs, including mammary glands, in female goats and sheep (Glascock and Hoekstra 1959) This finding...

Ngày tải lên: 23/03/2014, 17:20

... ERK and cell death: ERK1 ⁄ in neuronal death FEBS J Teixeiro E & Daniels MA (2009) ERK and cell death: ERK location and T cell selection FEBS J Martin P & Pognonec P (2009) ERK and cell death: ... of cell death that correlated with extensive cell rounding and the formation of cytoplasmic macrovacuoles, which pushed the nucleus and the cytoplasm to the side of the dying cell Although cell ... Journal compilation ª 2009 FEBS S Cagnol and J.-C Chambard ERK and cell death Table Continued Stimuli inducing cell death Duration of ERK activation promoting cell death Human melanoma C8161,WM164...

Ngày tải lên: 29/03/2014, 08:20

... hand, in non-neuronal cells, cytoplasmic retention of ERK1 ⁄ is required for death- associated protein kinase-mediated cell death [35] Thus, ERK1 ⁄ might promote cell death depending upon the cell ... glutamate-induced apoptotic cell death is mediated by ERK1 ⁄ and PI3-kinase pathways Cell Death Differ 12, 1329–1343 25 Subramaniam S, Shahani N, Strelau J, Laliberte C, Brandt R, Kaplan D & Unsicker K ... plasma membrane damage and independently of caspase-3 J Cell Biol 165, 357–369 35 Mebratu Y & Tesfaigzi Y (2009) How ERK1 ⁄ activation controls cell proliferation and cell death: is subcellular...

Ngày tải lên: 29/03/2014, 08:20

... determining cell fate decisions in a diverse number of organisms and cell types (reviewed in [7,8]) In thymocytes, c-JunNH2-terminal kinase (JNK) [9], p38 [10] and extracellular signal-regulated kinase ... negative selecting ligand Note the separate location of pJNK and pERK, and Ras-GRP1 ⁄ Grb2 ⁄ SOS ⁄ Ras ⁄ Raf at the plasma membrane lead to such distinct cell fates as survival and death Much is known ... this Mathematical modeling of LAT phosphorylation [36] and studies on the phosphorylation kinetics of individual LAT tyrosines [37], indicate that the lack of Grb2–LAT interaction during positive...

Ngày tải lên: 29/03/2014, 08:20

... ERK and cell death: cadmium toxicity P Martin and P Pognonec minutes of stimulation, followed by a second and more stable activation that can last for a few hours [11] The ... discrepancies, which could also be cell type related ERK activation ⁄ phosphorylation is the result of the balance between its phosphorylation by MEK and its dephosphorylation by dual-specificity phosphatases ... in a vast array of cellular signaling, including the activation of ERK [49] PMA mimics classical cell stimulation by growth factors, and PKC activation results in a strong and transient activation...

Ngày tải lên: 29/03/2014, 08:20