l 3 15256 with strong dendrite severing defects

... severing defects 35 3. 3.2 Mical promotes dendrite severing of ddaCs 36 3. 3 .3 Cell-autonomous function of Mical for dendrite severing 38 3. 3.4 Time-course analysis of EcR and Mical in dendrite ... mical15256/Df(3R)swp2MICAL hemizygotes and several transheterozygotes of mical15256 with previously published mical alleles At the WP stage, ddaCs in mical15256 (Fig 3D) as well as mical15256/Df(3R)swp2MICAL (Fig 3E) ... 9078 7 634 Df(3R)swp2Mical Mical 100% severing and clearance defects l( 3) 3911 67B3-67B4 N.D 997 7079 8970 N.D 100% severing and clearance defects l( 3) 116 73 87B11-87E8 92F7-93A7 cu-e 30 07 74 13 9088...

Ngày tải lên: 14/09/2015, 08:38

... BACE1 are regulated at multiple levels [25], including mRNA transcription, mRNA stability, glycosylation, 1504 proteolytic maturation, palmitoylation, and cellular localization Initial reports describing ... Authors Journal compilation ª 2010 FEBS C S Frigerio et al 31 32 33 34 35 36 37 38 39 40 dependent NRG1 ⁄ ErbB4 signaling and schizophrenialike phenotypes in BACE1-null mice Proc Natl Acad Sci USA ... (2002) Insulin-degrading enzyme rapidly removes the beta-amyloid precursor protein intracellular domain (AICD) J Biol Chem 277, 133 89– 133 93 54 Kimberly WT, Zheng JB, Town T, Flavell RA & Selkoe DJ...

Ngày tải lên: 15/03/2014, 10:20

... critical intellectual input ERK provided critical intellectual input Acknowledgements The authors thank Caroll Hartline and for titering virus samples, and Deb Collins for expert technical assistance ... removed, temporarily stored in sterile irrigating balanced salt solution, and homogenized in 1.0 ml MEM containing 10% FBS, mM L- glutamine, 200 U/ml penicillin, 50 µg/ml gentamycin and µg/ml fungizone ... A, Olson B, Talarico C, Davis M, Biron KK: Inhibition of ganciclovir-susceptible and -resistant human cytomegalovirus clinical isolates by the benzimidazole Lriboside 1263W94 Clin Diagn Lab Immunol...

Ngày tải lên: 20/06/2014, 01:20

... removal of HSPG from cell membranes, human tracheal epithelial HTE cell line, fetal human tracheal epithelial FHTE cell line, and cystic fibrosis IB3-1 cell line were treated with increasing amounts ... Immortalized human cell lines Human Tracheal Epithelial (HTE), Fetal Human Tracheal Epithelial (FHTE), and cystic fibrosis bronchial epithelial (IB3-1) [26] were cultured in LHC-8 basal media (Biofluids, ... in myocardial, pulmonary, and renal cells [4] Similar results have been noted in HeLa and CHO cells following infection by rAAV-2 with a capsid altered to be unable to bind to HSPG [3, 15] heparinase,...

Ngày tải lên: 20/06/2014, 01:20

... Egfrwa2/wa2 Total activitya Daytime activityb Maskingc 30 154 37 951 44485 235 09 51014 39 133 27 630 21821 40884 33 975 31 997 38 165 35 818 37 774 21888 439 30 46505 1.82 0.28 0.86 1. 43 1.44 0.74 30 .22 1 .30 0.08 ... homozygosity for Egfr null alleles results in lethality [1,10], while Egfrwa2 homozygous animals can survive to adulthood Both abnormally high diurnal activity and strong masking defects were reported ... shift resulting in abnormally high daytime activity, as well as abnormally high activity during a three hour light pulse (B) Abnormal wheel running activity during three-hour light pulses followed...

Ngày tải lên: 10/08/2014, 09:20

... polyclonal stable cell line in HEK2 93 cells stably expressing SUR1del146myc by selection with G418 and stable monoclonal cell lines expressing Kir6.2 + SUR1myc (line A), Kir6.2 + SUR1del101myc (line ... biochemical and subsequently electrophysiological methods Line C1 was labile with multiple passages often losing current Line C2 was more stable and could be passaged for long periods without 530 6 ... derived from an 8% polyacrylamide gel Lanes were loaded with lg of cellular homogenates of the stable lines indicated WT denotes untransfected HEK2 93 cells The positions of molecular mass markers...

Ngày tải lên: 31/03/2014, 08:20

... assessed using a clinical score graded from to (0, no swelling; 1, mild swelling of toes and ankle; 2, moderate swelling of toes and ankle; 3, severe swelling of toes and ankle) In addition, the ... strength at all) After cervical dislocation, the blood was withdrawn by heart puncture and the paws of all animals were dissected and preserved for histological analysis The last evaluation was ... central importance for mediating the proinflammatory effects of TNF-α Interestingly, all three MAPK families – p38 protein kinase, extracellular signal-regulated kinase, and c-Jun N-terminal kinase...

Ngày tải lên: 09/08/2014, 06:22

... hCycT1-tg n-tg hCycT1-tg 35 33 33 35 33 Rat ID n-tg 35 200 30 0 33 30 0 33 30 0 34 34 34 34 400 33 500 400 500 400 34 34 34 34 500 33 600 600 34 34 34 34 600 33 33 Mean Fluorescnece Intensity (MFI ... additional blocks for HIV replication in primary mouse T cells PLoS ONE 2008, 3( 4):e2 035 Cohen J: Building a small-animal model for AIDS, block by block Science 2001, 2 93( 5 532 ):1 034 -1 036 Keppler OT, ... gene expression relative to double-tg controls (on average ~2 .3- fold) and reached levels comparable to T-cells from the human donors (Fig 7A) In parallel, T-cells were challenged with the R5 HIV-1...

Ngày tải lên: 13/08/2014, 05:21

... CD4+enriched lymphocytes were plated at × 105 cells/well in 96-well plates with 5-fold serial dilutions of the virus The cells were fed on day with fresh media and scored on day 14 for p24 levels, with ... panel) Page of 15 (page number not for citation purposes) Retrovirology 2008, 5:10 http://www.retrovirology.com/content/5/1/10 A 2G12 b12 N 339 N392 N386 N386 C418 C418 A 433 A 433 N386 N392 N 339 N386 ... 68:4821-4828 http://www.retrovirology.com/content/5/1/10 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 Pantophlet R, Ollmann SE, Poignard P, Parren PW, Wilson IA, Burton DR: Fine mapping...

Ngày tải lên: 13/08/2014, 06:20

Ngày tải lên: 13/08/2014, 20:21

... viral release only at low levels of tetherin expression These results may explain why HIV-1 encoding S52A Vpu caused CD4+ T-cell depletion and replicated with wildtype-like efficiency in lymphoid ... measured cellular as well as released p24 by a quantitative Western blot two days later (Fig 2A and 2B) As expected, 293T cells expressing very low 10 ng (1:500) levels of tetherin released p24 ... in PBL and MDM from three different donors PBL were either left untreated or stimulated with μg/ ml PHA for 24 hours (PBL+) autologous T-cells (Fig 2C) Western blot analysis revealed multiple bands,...

Ngày tải lên: 12/08/2014, 23:22

... staining analyzed by flow cytometry including both, live and dead cells Black filled histogram: unstained cells, grey line: cells stained with isotype control antibody, grey filled histogram: cells stained ... that podoplanin, a cellular mucin expressed on kidney podocytes [37 ], type I alveolar cells and lymphoid endothelial cells [36 ], binds to CLEC-2 and activates CLEC-2-dependent signalling, suggested ... podoplanin produced in Lec1 cells still bound to CLEC-2 with appreciable efficiency (Fig 3C) In contrast, podoplanin produced in Lec2 cells and thus almost completely lacking sialoglycoconjugates did not...

Ngày tải lên: 12/08/2014, 23:23

... Dnop 53, pGFP-N-FUS, pRS-GAL-His-NOP 53 Dnop 53, pGFP-N-FUS-NOP 53 Dnop 53, YCp33GAL-A-NOP 53 NOP 53, YCp33GAL-A NOP 53, YCp33GAL-A-NOP 53 L4 0-41 L4 0-61 YFG- 131 YFG-247 YDG-146 YDG-147 YDG-148 NOP 53 Dnop 53 ... pGAD-NOP 53 YCp33GAL-A lexA DNA binding domain, TRP1 lm lexA::NIP7, TRP1 lm lexA::NOP17, TRP1 lm lexA::NOP 53, TRP1 lm GAL4::NOP8, LEU2 lm GAL4 activation domain, LEU2 lm GAL4::NOP17, LEU2 lm GAL4::NOP 53, ... ribosomal particles, FEBS Journal 272 (2005) 4450–44 63 ª 2005 FEBS D C Granato et al 28 29 30 31 32 33 34 35 36 37 38 39 associates with a subset of small nucleolar RNPs required for peptidyl transferase...

Ngày tải lên: 20/02/2014, 01:20

... per 106 cells) (U per 106 cells) F/R pLPL pLML pLVL pLEL + + + + + + + + + + + + GFP K 3L PKRD6 GFP K 3L PKRD6 GFP K 3L PKRD6 GFP K 3L PKRD6 Differentiated cells Renilla Firefly (U per 106 cells) (U per ... nondifferentiated cells Non-differentiated cells Differentiated cells Renilla (U per 106 cells) pLPL pLML pLVL pLEL pLL Firefly (U per 106 cells) F/R 59 94 89 78 52 7.5 6.4 42 3. 4 0.6 ± ± ± ± ± 3. 5 10 10 ... the overall protein synthesis level (A) Logarithmically growing (Log), density-induced growth arrested (Dense) or differentiated (Diff) K562 cells were metabolically labelled with [35 S ]L- methionine...

Ngày tải lên: 22/02/2014, 07:20

... and 0.2 lgÆmL)1 anhydrotetracycline for pASK-IBA vectors, followed by pulse labelling with [35 S]methionine (30 lCiÆmL)1) for 35 S labelling was stopped by adding an excess (15 mm) of cold methionine, ... during pulse labelling is inserted into the inner membrane, with its periplasmic loop properly located in the periplasm (Fig 2A) Upon lysis of the cells expressing MscL F54C, AMS labelling appeared ... panel shows a western blot of whole-cell samples using anti-Ffh serum to show the reduced levels of Ffh upon 4.5S RNA depletion The panel on the right shows western blot analysis of whole-cell...

Ngày tải lên: 07/03/2014, 02:20

... Biol Chem 2 73, 33 635 33 6 43 Gould SJ & Collins CS (2002) Opinion: peroxisomalprotein import: is it really that complex? Nat Rev Mol Cell Biol 3, 38 2 38 9 Lucau-Danila A, Delaveau T, Lelandais G, ... Substrate Activity Lpx1p PNB Acyl esterase Lpx1p DPG CRL DPG Lpx1p Lpx1p Lpx1p Lpx1p PPL DGR PC PC BPC BPC (Triacylglycerol) lipase (Triacylglycerol) lipase (Pancreas) lipase PLC PLD PLA PLA Activity ... together with 50 lL substrate-loaded liposomes Liposomes were prepared by injecting 90 lL of an ethanolic mixture of 3. 3 mm dioleyl phosphatidylcholine (Avanti Polar Lipids, Alabaster, AL, USA), 3. 3...

Ngày tải lên: 07/03/2014, 05:20

... by RLU used a subline of the HEK2 93 cell line, 2 93- K, which expresses caspase-8 at a level at least 10 times lower than that of HEK2 93 cells based on western blot analyses (Fig 4B and [ 43] ) Strikingly, ... pEF-BOS Other plasmids used in this study were described previously [10, 43] Cell lines HEK2 93 and 293T cell lines and the subline of HEK2 93, 2 93- K that expresses caspase-8 at a very low level were described ... HEK2 93 or 2 93- K cells were transiently transfected with 2·TRELuc, pRL-TK, and an expression plasmid for human Fas (100 ng), and cultured for 16 h The cells were then cultured with or without FasL...

Ngày tải lên: 07/03/2014, 09:20

... mm Na3VO4, lgÆmL)1 of leupeptin and lgÆmL)1 of aprotinin) The lysates were collected using a cell scraper and centrifuged for 10 at 130 00 rpm Samples from the lysates were collected and boiled ... Burgess-Brown NA, Muller S & Knapp S (2009) Large-scale structural analysis of the classical human protein tyrosine phosphatome Cell 136 , 35 2 36 3 10 Wang Y & Pallen CJ (1991) The receptor-like protein ... gel, blotted, probed with anti-Src Ig and developed with enhanced chemiluminescence (ECL) (middle panel) Part of the lysate was resolved on a 7.5% SDS-polyacrylamide gel, transferred to poly(vinylidene...

Ngày tải lên: 15/03/2014, 10:20

... are fully viable and fertile but display a clear phenotype When plants lacking PSI-J were transferred to soil, they grew photoautotrophically and were fully fertile (Fig 3) The original transformed ... Chl a (lg) Chl b (lg) Chl ⁄ P700 NADP+ photoreductiona [lmol NADP+Æs)1Æ(lmol P700))1] 1–qP 1–qP 1–qP a Leaf Leaf Leaf Leaf Thylakoids Thylakoids Growth chamber ⁄ growth light Greenhouse ⁄ cloudy ... photo-oxidation in solubilized thylakoid preparations from wild-type and DPSI-J plants lE, l moles photonÆm)2Æs)1 t1 ⁄ (ms) lE Wild-type n DPSI-J n 20 33 58 104.5 ± 3. 4 66 .3 ± 4.6 38 .0 ± 1.2 3 102.6 ± 12.8...

Ngày tải lên: 16/03/2014, 11:20

... in mature leaves Plant Cell 13, 1 437 –1452 31 Egelkrout EM, Mariconti L, Settlage SB, Cella R, Robertson D & Hanley-Bowdoin L (2002) Two E2F elements regulate the proliferating cell nuclear antigen ... p35SRepA; lane 3, p35Srep; lane 4, p35Dintron + p35SRepA; lane 5, p35SDintronRBR–; lane 6, p35DRepARBR–; lane 7, p35SCP; lane 8, p35SDBRep Samples were collected 24 h after cobombardment Luciferase ... bombarded with p35SHA6HISRep alone; lane 2, extracts from cells bombarded with p35SHARepA alone; lane 3, extracts from cells bombarded with both p35SHA6HISRep and p35SHARepA after the first wash; lane...

Ngày tải lên: 16/03/2014, 13:20