kiểm tra vị trí đóng hay cắt của thiết bị có liên quan đến các thao tác đang tiến hành có đúng với mạch khoá liên động không
diffusion equations with arbitrary polynomial growth
Ngày tải lên :
29/10/2015, 14:20
... attractor M for the map L automatically implies the existence of the global attractor A and the embedding A ⊂ M We note however that, in contrast to the global attractor, an exponential attractor ... attractor in a stronger topology space In Section 3, we prove the existence of exponential attractors in L2 (Ω ) with g ∈ H −1 (Ω ), i.e., Theorem 3.1 In Section 4, we obtain an exponential attractor ... of a global attractor in L2 (Ω ), H (Ω ), L2p−2 (Ω ) with arbitrary polynomial growth nonlinearity and g ∈ L2 (Ω ) Let us come back to our problem First, to obtain an exponential attractor in L2...
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Báo cáo khoa học: Ion-binding properties of Calnuc, Ca2+ versus Mg2+ – Calnuc adopts additional and unusual Ca2+-binding sites upon interaction with G-protein pdf
Ngày tải lên :
07/03/2014, 00:20
... to the Mg2+:dye:protein complex The concentration of metal ions was mM All spectra were recorded in 20 mM Tris ⁄ HCl and 50 mM NaCl (pH 8.0) The spectra were recorded with nm slits on the excitation ... representative spectra that were repeated several times All experiments were performed at ambient room temperature (25 °C) in a final volume of mL Buffer blanks have been subtracted from these spectra tryptophan ... the presence of mm Mg2+, mm Ca2+, and 50 lm GDP (Fig 5) Buffer–Calnuc titration was subtracted from G-protein–Calnuc titration isotherm data to take into account the heat of dilution Binding...
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Báo cáo khoa học: C-terminal truncated cannabinoid receptor 1 coexpressed with G protein trimer in Sf9 cells exists in a precoupled state and shows constitutive activity ppt
Ngày tải lên :
23/03/2014, 07:20
... activity, thereby transducing the biological signal, even in the absence of ligand [18] Truncation of the distal C-terminal tail has been shown to enhance the constitutive activity and sequestration ability ... of a 1· concentration of Sf9 membranes or CB1 membranes, respectively (The bullets used in this figure are not data points, but are used to distinguish between the different spectra.) used in ... calculate the receptor concentration in pmolÆmg)1 A Kaleida graph (Synergy software) was used to plot the receptor binding sites versus radioactive ligand concentration in a nonlinear regression...
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báo cáo khoa học: "HLA-matched sibling transplantation with G-CSF mobilized PBSCs and BM decreases GVHD in adult patients with severe aplastic anemia" pdf
Ngày tải lên :
10/08/2014, 22:21
... and peripheral blood stem cell translantation from HLA-matched donors References Remberger M, Naseh N, Aschan J, et al: G-CSF given after haematopoietic stem cell transplantation using HLA-identical ... Grover, James Lovato, Suzy V Toirti, Frank M Torti, Istvan Molnar: The transplant iron score as a predictor of stem cell transplant survival Journal of Hematology & Oncology 2009, 2:44 Zi-Min ... AL, Horn B, et al: Primary graft failure after umbilical cord blood transplant rescued by parental haplocompatible stem cell transplantation J Pediatr Hematol Oncol 2009, 31(4):300-3 Min CK, Kim...
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Synthesis and structure investigation of stabilized aromatic oligoamides and their interaction with g quadruplex structures 1
Ngày tải lên :
10/09/2015, 15:49
... linearity due to the formation of the hydrogen bonds on both sides of the molecular strand In contrast, some linear strands are not expected to be strictly linear and a curvature in backbone could ... 1.7) As expected, this class of macrocycle was demonstrated to form transmembrane channel with very large conductance and a model of the transport pore was prompted as 10 Figure 1.7b.24 Figure ... three-center H-bonds in rigidifying the oligoamide backbone was demonstrated by the well-defined crescent conformation of dimer, trimer and tetramer According on this three-center H-bonding, a 9-mer and...
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Synthesis and structure investigation of stabilized aromatic oligoamides and their interaction with g quadruplex structures 2
Ngày tải lên :
10/09/2015, 15:49
... (H2SO4) into conc nitric acid containing compounds to be nitrated), the nitration method using montmorillonite (MMT) impregnated with bismuth nitrate (Bi(NO3)3) was finally singled out The condition ... very mild, simply involving mixing the compounds to be nitrated (1b-1d) with Montmorillonite K10 impregnated with bismuth nitrate in Tetrahydrofuran (THF) at room temperature and stirring the ... 2-hydroxy-5-methylbenzoic acid, bismuth nitrate-mediated nitration at room temperature tends to give inconsistent low chemical yields from time to time It was finally realized that such nitration is highly sensitive...
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Synthesis and structure investigation of stabilized aromatic oligoamides and their interaction with g quadruplex structures 3
Ngày tải lên :
10/09/2015, 15:49
... computational molecular modeling at the level of B3LYP/6-31G* on 5a showed that oligomers of higher than tetramer should take up a helical backbone rather than the planar conformation as proposed previously.3c,5a ... in the formation of a continuous internally located H-bonding network that comprises up to ten intramolecular H-bonds (NH…OMe = 1.933-2.306 Å) One disparity between 5a and 6a involves the helical ... most conclusive evidence for the adoption of a helical conformation by the foldable molecular strands 5a and 6a, (2) validate our conceptual reasoning and others’ observations1f,2c detailing...
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Synthesis and structure investigation of stabilized aromatic oligoamides and their interaction with g quadruplex structures 4
Ngày tải lên :
10/09/2015, 15:49
... To study the intramolecular H-bonds in 8, 1H NMR spectra was first examined As mentioned, amide protons typically exhibit a substantial downfield shift due to the formation of intramolecular H-bonds ... demonstrated chemical shift more than 10 ppm as a result of the H-bonding ability of DMSO 4.2.7 Crystal Structure of a Tetramer and Computational Molecular Modeling of Circular Pentamer To demonstrate ... demonstrate the existence of intramolecular hydrogen-bonds that restrict the rotational freedom of the aryl-amide bonds to enforce a crescent structure, X-ray crystallography of tetramer 8h was obtained...
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Synthesis and structure investigation of stabilized aromatic oligoamides and their interaction with g quadruplex structures 5
Ngày tải lên :
10/09/2015, 15:49
... ml), the aqueous layer was neutralized by addition 1M HCl (1.0 mL) The mixture was extracted with CHCl3 (3x15 mL) The organic extracts were dried over NaSO4 and concentrated under reduced pressure ... ml), the aqueous layer was neutralized by addition 1M HCl (1.5 mL) The mixture was extracted with CHCl3 (3x15 mL) The organic extracts were dried over NaSO4 and concentrated under reduced pressure ... ml), the aqueous layer was neutralized by addition 1M HCl (1.5 mL) The mixture was extracted with CHCl3 (3x15 mL) The organic extracts were dried over NaSO4 and concentrated under reduced pressure...
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Synthesis and structure investigation of stabilized aromatic oligoamides and their interaction with g quadruplex structures 6
Ngày tải lên :
10/09/2015, 15:49
... oligonucleotide From the red curve and the difference curve after subtraction of red curve from the black one, we can deduce the concentrations of the gold particles and the aptamers from the absorbance ... extinction coefficient, c is molar concentration, and l is path length Given Eapt29 =143300 M-1cm-1 and EAu =105614000 M-1cm-1 From Figure 6.1, the concentrations of AuNPs and aptamer are 4.5 nM ... -0.05 300 400 500 600 700 800 Wavelength (nm) Figure 6.4 UV-Vis spectra of Au-T5 and that with added thrombin of different concentrations Surprisingly, both the solution color change and the UV...
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Synthesis and structure investigation of stabilized aromatic oligoamides and their interaction with g quadruplex structures 7
Ngày tải lên :
10/09/2015, 15:49
... 10a on the double-stranded PCR product synthesized from (a) TBA template, (b) Control DNA which can not form G-quadruplexes structure ds = double-stranded and ss = single-stranded Next, the feasibility ... and 11a on the double-stranded PCR product synthesized from TBA template As shown in Figure 7.10, when the concentrations of 10a, 11a, 10b were lower than μM, double-stranded DNA PCR product ... contrast, small molecules which are not G-quadruplex ligands will not disaggregate AuNPs since the association between protein and TBA is not disrupted by small molecules 197 Figure 7.1 Illustration...
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.OVER THE TOP WITH THE THIRD AUSTRALIAN DIVISIONBY G.P. CUTTRISSWITH INTRODUCTION BY ppt
Ngày tải lên :
06/03/2014, 03:21
... omissions will not detract from the interest of the book 'Over the Top with the Third Australian Division' is illustrative of that big-hearted, devil-maycare style of the Australians, the men who ... Third Australian Division G.P CUTTRISS The Author Photo by Lafayette, Ltd INTRODUCTION At the outbreak of the World War in August, 1914, the Australian as a soldier was an unknown quantity ... South Africa, Australia had been represented, and that a sprinkling of native-born Australians had taken service in the Imperial armies The performances of these pioneers of Australia in arms were...
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
Ngày tải lên :
07/03/2014, 17:20
... interactions, the peptides were titrated with both neutral and negatively charged vesicles Titration of the 2457 HGV ⁄ GBV-C fusion peptide C Larios et al peptides with neutral 1-palmitoyl-2-oleoylphosphatidylcholine ... concentration, F0 the fluorescence intensity at the beginning of the titration, F1 the fluorescence at the end of the titration, Kd the dissociation constant, and [Ltot] the total lipid concentration ... PtdCho concentration was determined by an enzymatic ´ colorimetric assay (bioMerieux), and total phospholipid concentration was determined by phosphorus analysis [29] Trp fluorescence titrations Fluorescence...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
Ngày tải lên :
16/03/2014, 13:20
... final concentration of 0.5 lM recording the spectra at 37°C as described in Experimental procedures (B) GRO26A and GT-G4 were diluted to 0.5 lM final concentration in water and the spectra were recorded ... illustrated in Fig 6A, the weak CD bands of GT and GT-G1 indicate absence of appreciable secondary structure at 37 °C, under conditions similar to those of the extracellular medium The spectra ... significantly alter cellular proliferation On the contrary, in our experimental conditions, which were similar to those of the extracellular medium, the CD spectra of GRO26A show that it formed a stable...
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Báo cáo khoa học: G protein-coupled receptor 30 down-regulates cofactor expression and interferes with the transcriptional activity of glucocorticoid pdf
Ngày tải lên :
16/03/2014, 18:20
... between the GPR30-transfected and vectortransfected samples, were arrived at as follows: Ngene ¼ ðTIF2GPR30-transfected =TBPGPR30-transfected Þ= ðTIF2vector-transfected =TBPvector-transfected Þ: ... supplemented with 5% fetal bovine serum and penicillin/streptomycin In transient transfection assays, fetal bovine serum was replaced by 10% dextran-coated, charcoal-stripped fetal bovine serum RNA isolation ... and transferred to a nitrocellulose membrane with an electrophoretic transfer apparatus After blocking, the membranes were incubated overnight at °C with GRIP-1 (F-20) antibody at a final concentration...
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Báo cáo khoa học: The study of G-protein coupled receptor oligomerization with computational modeling and bioinformatics doc
Ngày tải lên :
16/03/2014, 22:20
... interactions in GPCR function (e.g transactivation) Methods employed The evolutionary trace method The evolutionary trace method is an adaptation of an earlier strategy for the hierarchical analysis ... evolutionary trace method Intl J Quantum Chem Biophys Q74, 371–379 Filizola M, Olmea O & Weinstein H (2002) Prediction of heterodimerization interfaces of G-protein coupled receptors with a new subtractive ... functional interfaces) in proteins of known structure The set of assumptions used to extract such evolutionary trace residues from sequence conservation patters in homologous proteins includes the...
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Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
Ngày tải lên :
23/03/2014, 13:20
... concentration of KCl (100 mM) used in normal reactions and varying concentrations of MgCl2 (0–50 mM), or of a standard concentration of MgCl2 (5 mM) used in normal reactions and varying concentrations ... Tetrahymena telomeric sequence (Tet-tel) and Tel6Rev (C-strand telomeric sequence) as single-stranded oligoprobes, together with a partial duplex DNA containing a 3¢ G-overhang and a double-stranded ... in the absence of extract were performed with 100 mM KCl (standard concentration used in normal reactions) and varying concentrations of MgCl2 (0–50 mM) although other concentrations were also...
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Asthma WellnessKeeping Children with Asthma in School and Learning Liability & Litigation: A Legal Primer Asthma & Indoor Air Quality (IAQ)Asthma Management, Policies and Procedures.St r a i g h tBy Paul D. Houstont a l kIn School and Healthy: docx
Ngày tải lên :
23/03/2014, 23:20
... achievement and ascertain specific strategies to identify and address this Paul D Houston is executive director of the American Association of School Administrators issue in your school districts ... contents Spring 2003 A School Board Publication of the American Association of School Administrators Straight Talk features Resources 16 Cover Story: A Childhood Epidemic Asthma Management, Policies ... Leaders Can Do The CDC has identified six strategies for addressing asthma as part of a coordinated school health program (CDC, 2002b) Note that every strategy may not be feasible for your district...
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