katrin erk and joachim niehren

... PD098059 and U0126, were validated by analysis of phosphorylation of ERKs 1 and 2, which lie downstream of MEK1 [24]. Both TNFa and IL-1b upregulated phosphorylation of p42/44 ERKs and preincubation ... DNA binding and NF-jB-dependent transcription To examine the role of the p38 and the ERK MAPK pathways, we examined the effects of the p38 MAPK inhibitor, SB203580, the MEK1 inhibitor, PD098059 and the ... inhibition of the ERK pathway, with the MEK1 inhibitor, PD098059, abrogated IKK activity and dominant negative constructs of ERK prevented NF-jB DNA binding activity [12,13]. In addition, the ERK pathway...

Ngày tải lên: 07/03/2014, 15:20

... 50-fold compared to the standard assay) and much longer incubation times (approximately 200-fold) because of excellent enzyme expression and simple purification. The isolated and purified glucosidic ... concentration and activity Protein concentrations were measured using the method of Bradford [5] and a standard curve derived from bovine serum albumin. For testing the activity and determining ... discussed in the literature as important for enzyme activity and were also far from the NRDs and the His-Glu domains; Lys86 was changed to Ile and Ala204 to Val (Fig. 2, Table 1). The results indicated...

Ngày tải lên: 17/03/2014, 09:20

... 810–816. S. Cagnol and J C. Chambard ERK and cell death FEBS Journal 277 (2010) 2–21 ª 2009 The Authors Journal compilation ª 2009 FEBS 21 MINIREVIEW ERK and cell death: Mechanisms of ERK- induced cell death ... Developmental Biology and Cancer, University of Nice, France Ras ⁄ Raf ⁄ ERK, the pathway ERK2 ⁄ ERK1 (also known as p42 ⁄ p44MAPK, respec- tively, and officially named MAPK 1 and 3) are two isoforms ... the ERK1 ⁄ 2 pathway. Cell Death Differ 16, 368–377. 6 Subramaniam S & Unsicker K (2009) ERK and cell death: ERK1 ⁄ 2 in neuronal death. FEBS J. 7 Teixeiro E & Daniels MA (2009) ERK and...

Ngày tải lên: 29/03/2014, 08:20

Ngày tải lên: 29/03/2014, 08:20

Ngày tải lên: 29/03/2014, 08:20

Ngày tải lên: 29/03/2014, 08:20

Ngày tải lên: 21/06/2014, 19:20

Ngày tải lên: 22/06/2014, 22:20

... of E-cadherin via Src- and Shc-dependent Erk signaling Sandra Kleiner, Amir Faisal* and Yoshikuni Nagamine Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland The major cancer-associated ... E-cadherin distribution, cell scattering and uPA expression. (A) T47D cells (a and b) and MCF7 cells (c and d) were treated for 4 h with control or Decma supernatant and immunostained with anti-E-cadherin ... and b) and MCF7 cells (c and d) were grown for 2 days to 60–70% confluence and then treated with control or Decma supernatant for 6 h before recording. (C) MCF7 cells were treated with Decma and...

Ngày tải lên: 19/02/2014, 05:20

... immunoreactivity of ERK1 /2 and phospho ERK1 /2 (A,B), and MEK1/2 and phospho MEK1/2 (C,D). The lower part of each panel reports the quantitative analysis of phospho ERK1 /2 (A,B), and phospho MEK1/2 ... (individual densities from ERK1 and 2 bands were added up to generate one single ERK1 /2 value). Fig. 3. NAMI-A inhibits MEK1/2 activation and GF109203X down regulates ERK1 /2 phosphorylation. (A,B) ... MEK1/ 2, ERK- 1/2, phospho-MEK1/2 and phospho -ERK1 /2 (NewEnglandBiolabs,Inc.Beverly,MA,USA).The following dilutions were used for individual antibodies against different proteins: MEK1/2 and ERK1 /2...

Ngày tải lên: 21/02/2014, 01:21

... neokyotorphin requires Ca 2+ influx and activation of PKA, CaMK II and MAPK /ERK Olga V. Sazonova, Elena Yu. Blishchenko, Anna G. Tolmazova, Dmitry P. Khachin, Konstantin V. Leontiev, Andrey A. Karelin and Vadim T. ... 8-Br- cAMP and neokyotorphin showed only modest and poorly reproducible suppressive effects. Swiss 3T3 fibroblasts enhanced their proliferation upon PKC activation and did not respond to 8-Br-cAMP and neokyotorphin. Neokyotorphin ... FEBS H-89 was used to inhibit PKA activation. It inhibits PKA I and II (IC 50 ¼ 135 nm), and also ribosomal S6 kinase 1 (S6K1) and mitogen- and stress-activated pro- tein kinase 1 (MSK1); with 10 lm...

Ngày tải lên: 07/03/2014, 11:20

... such as the MAPK pathway and that distin- guishing between the early amplitude and late plateau, and duration and area-under-the-curve may be import- ant for understanding differential control ... duration and integral strength are introduced. We then identify and prove new principles, such that total control on signal ampli- tude and on final signal strength must amount to zero, and total ... decision between growth and differentiation are discussed. Abbreviations EGF, epidermal growth factor; ERK, extracellular signal-regulated kinase; ERK- PP, doubly phosphorylated ERK; MAP(K), mitogen-activated protein...

Ngày tải lên: 16/03/2014, 18:20

... lysates were collected and proteins separated by 1D SDS/PAGE, western blotted and probed with anti-phospho -ERK IgGs (pERK). Blots were stripped and reprobed with an anti -ERK IgG (ERK) as a loading ... proteins separated by 1D SDS/PAGE, western blotted and probed with anti-phospho -ERK IgGs (pERK). Blots were stripped and reprobed with an anti -ERK IgG (ERK) as a loading control. (C) M1/WT cells were ... CSF-1R-mediated macrophage differentiation and ERK activation Nicholas J. Wilson 1, *, Maddalena Cross 3 , Thao Nguyen 3 and John A. Hamilton 1,2,3 1 Arthritis and Inflammation Research Centre, Department...

Ngày tải lên: 16/03/2014, 22:20

... mediated by the following kinases; p44/42 MAPK /ERK, p38 MAPK, and c-jun N-terminal kinase (JNK). It is reported that Elk-1 and Atf-2 are phosphorylated by ERK and p38 MAPK, respec- tively [30,33,34]. In ... temparature and then incubated with anti -ERK Ig, anti-(active -ERK) Ig, anti-(p38 MAP) Ig or anti-(active-p38 MAPK) Ig (New England BioLaboratories, Bevely, MA, USA, Calbiochemistry, San Diego, CA, USA and ... plasmid, pTet-Elk (A) and pTet-Atf (B) and the reporter plasmid, pTRE-luc, as described in Materials and methods. At 24 h after transfection, the cells were serum- deprived for 24 h, and incubated with...

Ngày tải lên: 24/03/2014, 04:21

Ngày tải lên: 30/03/2014, 11:20

Ngày tải lên: 20/06/2014, 07:20

... will Ⅲ Understand the embedded Linux development environment. Ⅲ Understand and create Linux BSP for a hardware platform. Ⅲ Understand the Linux model for embedded storage and write drivers and applications ... leaks and memory corruption in applica- tions and drivers. Ⅲ Learn methods to profile applications and the kernel. Ⅲ Understand uCLinux architecture and its programming model. Ⅲ Understand the ... I2C subsystem, and USB gadgets. vi Embedded Linux System Design and Development Ⅲ Borland is a registered trademark of Borland Software Corporation in the United States and other countries. ...

Ngày tải lên: 04/08/2012, 14:23

... during transport and handling – particle and ingredient segregation (separation) occurs when particles of different sizes and bulk densities are blended together and transported or handled. 4. ... Size, Bulk Density and pH Particle size and particle size uniformity of feed ingredients are important considerations of livestock and poultry nutritionists when selecting sources and determining ... of U.S. DDGS Physical and chemical characteristics of distiller’s dried grains with solubles (DDGS) vary among sources and can influence its feeding value and handling characteristics. These...

Ngày tải lên: 08/08/2012, 10:03

Ngày tải lên: 08/08/2012, 17:20

... products and how they work, how the distribution system operates, how to deal with both suppliers and customers and how to handle complaints. They also study trade and retail laws, and are accompanied ... solve problems and find resol that are prac and bene to everyone. 9. Unlike a formal court case, nego are in private. 10. Resolutions and sett are based on com and on mut agreement and acceptance. 11. ... jobs and develop their skills for the whole period they are working here, and not just at the beginning of their contract. We run regular courses and workshops in order to achieve this, both on and...

Ngày tải lên: 08/08/2012, 17:31