k thut rapd random amplified of polymorphic dna

tiểu luận kỹ thuật di truyền- kỹ thuật rapd (random amplified polymorphic dna)

Ngày tải lên : 06/01/2015, 21:51

... điểm k thuật RAPD: Ưu điểm: _ Về mặt k thuật: + K thuật RAPD dễ thực dễ thành công không cần biết trước trình tự gene đối tượng cần nghiên cứu + Thao tác đơn giản + Chất lượng DNA khuôn không ... cần độ tinh cao + Thời gian thực nhanh Khả nhân cao _ Về mặt kinh tế: + Chi phí thực thấp + K thuật RAPD thường sử dụng k t hợp với k thuật cao cấp khác để đánh giá đa dạng di truyền nhận ... quan kiểu gene kiểu hình phản ánh bệnh đạo ôn số giống lúa Việt Nam VI Những cải tiến k thuật RAPD: + K thuật thứ nhất: sử dụng lúc primer khác thay sử dụng primer k thuật RAPD thông thường...

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Báo cáo khoa học: "Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India" docx

Ngày tải lên : 07/08/2014, 18:21

... lositroc dna niportocitroconerda ,enimalohcetac amsalP K ayaT ,M iaK ,A agariH ,K amiM ,F adekaT ,S atagaN ,M awasoruK 22 0002 ,drofgnillaW ,lanoitanretnI BAC ,704-583 pp de dn2 tropsnarT dna gnildnaH ... eht yb desuac raef dna ,]23,61[ gnildnah yb noissergga lacisyhp dna gnituohs dna ,]83,92,02[ slamina rof ytlevon a si nep gnildnah ehT sserts lanoitidda esopmi ]32,71,61,9[ gnildnah gnirud elpoep ... diocitrococulg laceF RL swehttaM ,AG krekreV ,SE revloK ,JC worroM 92 332-822 ,08 ,2002 J teV tsuA serudecorp yrdnabsuh lufniap ot sevlac dna sbmal gnuoy fo sesnopser lositroc dna enimalohcetac fo nosirapmoc...

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Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"

Ngày tải lên : 25/10/2012, 11:18

... active at the site of single strand DNA breaks, and single-strand nicking is a known activity of NS1 protein, it is likely that NS1 is nicking the cellular DNA, thereby inducing DNA damage This ... multiple types of DNA lesions and that are often caused by collapsed replication forks (44) NS1 could easily cause double strand breaks through the simple mechanism of nicking both DNA strands a ... are suggestive of DNA damage as a cause of NS1-induced apoptosis ATM principally binds to free DNA ends or DNA strand breaks (43), while ATR recognizes single-stranded regions of DNA common to...

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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx

Ngày tải lên : 19/02/2014, 16:20

... ()7.8 kb to )6.0 kb; DHSs, )7.3 kb and )6.5 kb); region II, promoter and upstream region of Ig-b gene ()2.7 kb to +0.05 kb; DHSs, )2.1 kb, )1.0 kb, and kb); region III, in the ﬁrst intron of the ... (+0.6 kb to +1.1 kb; DHS, +0.9 kb); region IV, between Ig-b and GH genes (+4.5 kb to +6.7 kb; DHSs, +4.8 kb, +5.2 kb, +5.8 kb, and +6.1 kb) Dele423 Deletion of hypersensitive sites in chicken Ig-b ... loxP vectors for selectable marker recycle and conditional knock-outs BMC Biotechnol 1, Tanaka M, Hosokawa Y, Watahiki M & Nakashima K (1992) Structure of the chicken growth hormone-encoding gene...

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Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

Ngày tải lên : 20/02/2014, 11:20

... addition of a TPP-moiety has been used to drive the selective uptake of a wide variety of Ó FEBS 2003 Fig Selective uptake of mitoDC-81 by mitochondria and subsequent alkylation of mtDNA The uptake of ... chloroform-d1 standardized against the solvent peak or external Circular plasmid DNA (5 lg; derived from pYES-PA2, 6.8 kb), HindIII digested k DNA (5 lg), or a ladder of linear marker DNA (5 lg of ... mitoDC81 alkylates mtDNA in mitochondria or cells The reasons for the lack of alkylation of mtDNA within mitochondria by mitoDC-81 are unclear The local concentrations of mitoDC-81 and DNA, and...

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Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc

Ngày tải lên : 20/02/2014, 11:20

... inhibition of DNA alkylation, S23906-1 (50 lM) was ﬁrst incubated with an excess of GSH or derivatives (400 lM) in 20 lL of ammonium acetate for h at 37 °C prior to the addition of the radiolabeled DNA ... step size of 0.1 Da and a dwell time of ms Twenty spectra were summed and recorded at an oriﬁce voltage of +50 V, whereas the potential of the spray needle was held at +5 kV Alkylation of plasmids ... separated from DNA cross-linked molecules by phenol/chloroform/isoamyl alcohol (25 : 24 : 1) extraction followed by addition of lL of 5-M NaCl and precipitation of DNA using mL of cold ethanol After...

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Báo cáo khoa học: The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III s subunit ppt

Ngày tải lên : 05/03/2014, 23:20

... C-terminus of the tau subunit of Escherichia coli DNA polymerase III holoenzyme is the site of interaction with the alpha subunit Nucleic Acids Res 35, 2813–2824 Katoh K, Misawa K, Kuma K & Miyata ... albeit weakly (hhsearch probability of 16%) The structures of PolIIIs-V and DnaA-I adopt a variant of the so-called type II KH fold [21] One of their major differences from classical type II KH domains ... PolC and DnaE The ability to bind single-stranded DNA has indeed been demonstrated for the E coli DnaE OB domain [12,16] The very N-terminal region of PolC and the C-terminal domain of DnaE appear...

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Báo cáo khoa học: Speciﬁc cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot

Ngày tải lên : 06/03/2014, 22:21

... head of bacteriophage T4 Nature 227, 680–685 Kuznetsova IM, Biktashev AG, Khaitlina SY, Vassilenko KS, Turoverov KK & Uversky VN (1999) Effect of self-association on the structural organization of ... 15 16 17 18 19 of nucleotide dissociation and exchange rates J Biol Chem 268, 8683–8691 Strzelecka-Golaszewska H, Moraczewska J, Khaitlina SYu & Mossakowska M (1993) Localization of the tightly ... proteolysis of actin by a speciﬁc bacterial protease FEBS Lett 228, 172–174 Thermal unfolding of cleaved actin 20 Khaitlina SYu, Collins JH, Kuznetsova IM, Pershina VP, Synakevich IG, Turoverov KK &...

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Báo cáo khoa học: Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria doc

Ngày tải lên : 07/03/2014, 00:20

... center and alter the kinetics of interaction with the isoforms of yeast cytochrome c J Biol Chem 270, 110–118 Yoshikawa S, Shinzawa-Itoh K & Tsukihara T (1998) Crystal structure of bovine heart cytochrome ... supercomplex (CIII2 ⁄ CIV) forms of complex IV, with the additional presence of an approximately 250 kDa complex (marked with an asterisk) that resolved after 10 of import (Fig 2A, lanes 3, 4, ... the core I subunit of complex III (a-complex III) and the COI subunit of complex IV (a-complex IV) The asterisk indicates a complex of approximately 250 kDa complex forms of complex IV, mitochondria...

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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

Ngày tải lên : 07/03/2014, 17:20

... cycle catalyzed by UAS DNA Cell 86, 331–339 North AK, Klose KE, Stedman KM & Kustu S (1993) Prokaryotic enhancer-binding proteins reﬂect eukaryotelike modularity: the puzzle of nitrogen regulatory ... ⁄ PAGE of the soluble cell extracts of E coli BL21 (pHB171) showed a protein of 43 kDa (gel not shown), which corresponds to the molecular mass of DA-HbpR (39 kDa) plus that of CBP (4 kDa) This ... become more prone to DNaseI attack Within the limits of the resolution of the DNaseI mapping, it was also observed that loss of protection always extended in two regions (like R12 and R13), except...

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Molecular base of Inheritance DNAPremedical - biology

Ngày tải lên : 13/03/2014, 16:57

... oxidation of fatty acids • the oxidation of pyruvate in the citric acid cycle Alternate DNA structures DNA exists in many possible conformations that include A -DNA, B -DNA, and Z -DNA forms only B -DNA ... discontinuously - Series of segments = Okazaki fragments enzyme DNA Ligase joins them Primase RNA primers Helicase Correction of mistakes • One /10 000bp in replication • DNA polymerase • special ... Proof that DNA is carrier of genetic information - 1928 • Griffith‘s experiment - Bacterial transformation • Recombination of bacterial genetic material by transmission of naked DNA into...

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Báo cáo khoa học: Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals pdf

Ngày tải lên : 15/03/2014, 11:20

... DUT-NLSD(10)13,17)18)–YFP PEKRKQE; KPKRKKKR None PEKRKQE PEKRKQE; KPKRAAKR PEKRKQE; KPKRAAAA PEKRKQE; KPKRKKKR PEKRKQE; KPKRAAAA PEKRKQE KPKRKKKR KPKR None PAAKKMKID MKID PAAKKMKID KKMKID KMKID PAAKKMK KKMK KMK + ) ... segments KPKR (346) PKRK (347) KRKK (348) RKKK (349) KKKR (350) PEKRKQE (320) PKRKKKR (347) PAAKKMK (10) PKKR (711) RKKK (716) RKKH (760) KKKR (1088) RPKK (1093) PKKK (1141) KKKR (1142) RPKK (1147) ... Nucleoplasmin NLS sequence PKKKRKV PKRKKKR PKKRGRKKK SKSRKRKL PSKRARP GKKRSKV PAAKRVKLD PAAKKMKID VSSKRAKLE PPVKRERTS KRPAATKKAGQAKKKKLDK signals were observed Among these, the PKKRG RKKK(711–719) sequence...

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Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf

Ngày tải lên : 16/03/2014, 10:20

... and interact with short DNA sequences in the presence of a vast excess of nonspeciﬁc DNA [1] In such situations, it is likely that small differences in reaction afﬁnity, or kinetics, can deeply ... in the DNA recognition kinetics and in the stability of the complex [22], suggesting that there is an involvement of this loop in HPV-16 E2 DNA recognition Another interesting aspect of HPV-16 ... the presence of Lys349 in the loop connecting helix a2 and strand b4 This residue may have a role in DNA binding, as a single mutation of Lys349 to alanine weakens the DNA binding of the HPV-16...

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Báo cáo khoa học: Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain pdf

Ngày tải lên : 16/03/2014, 22:20

... dissociation and binding constants k+ 1 and k) 1 were obtained at 25 °C A k+ 1 (M)1Æs)1) Kd (lM) DnaK KKCC KCCC mtHsp70 B C Fig Peptide interaction properties of DnaK, mtHsp70 and chimeras (A) F-APPY ... steady-state ATPase activities of KKCC and KCCC were comparable with that of DnaK, however, addition of the NR peptide poorly enhanced their activity (less than twofold) Bacterial co-chaperones ... structure for DnaK and mtHsp70 [35], and also indicates that sequence exchange at the PBD of DnaK did not modify the overall secondary structure of the chimeras The intrinsic ﬂuorescence of DnaK has...

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Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

Ngày tải lên : 23/03/2014, 13:20

... for slipped DNA Biochemistry 37, 9426–9434 23 Mizukoshi, T., Tanaka, T., Arai, K. , Kohda, D & Masai, H (2003) A critical role of the 3¢ terminus of nascent DNA chains in recognition of stalled ... for measuring a single type of DNA hairpin’s reopening temperature embedded in a DNA molecule has been developed in this work The method utilizes the knowledge of DNA hairpin structure formation ... the T4 DNA polymerase from carrying out primer extension at 22 °C Fast annealing may decrease the likelihood of the self-folding of the template DNA, while it may increase the probability of primer...

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Báo cáo Y học: The N-terminus of m5C-DNA methyltransferase Msp I is involved in its topoisomerase activity docx

Ngày tải lên : 24/03/2014, 00:21

... Venetianer, P & Kiss, A (1994) Self-methylation of BspRI DNA- methyltransferase Nucleic Acids Res 22, 2876–2881 17 Bhattacharya, S .K & Dubey, A .K (1999) Kinetic mechanism of cytosine DNA methyltransferase ... cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment Proc Natl Acad Sci USA 85, 2543– 2547 30 Barnes, D.E., Johnston, L.H., Kodama, K. , Tomkinson, ... acids of fragment p26 are LKLIRSKLDLTQK QA The sequence obtained for the ﬁrst 20 amino acids of p18 is GIPQKRKRFYLVAFLNQNIH This is in agreement with the C-terminal portion of M.MspI that would contain...

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Báo cáo Y học: ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1 pot

Ngày tải lên : 24/03/2014, 00:21

... cdk inhibitor p21, suggesting that MDM2 activation of E2F1 occurs as a consequence of inhibition of p53 transactivation of p21 [40] Upon overexpression of MDM2, p53 transactivation is blocked, ... SD of duplicate experiments) (G,H) Analysis of E2F1 DNA binding using increasing concentrations of wild-type MDM2 or MDM2 -K4 54A and ATP GST–E2F1 protein (100 ng) was incubated in the presence of ... presence of E2F1 antibody KH95 (200 ng, lane 3), and DNA- binding reactions were analysed using native gel electrophoresis (B) Analysis of E2F1 DNA binding using increasing concentrations of wild-type...

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Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf

Ngày tải lên : 30/03/2014, 11:20

... abundance of a-satellite and satellite III RNA in KD-1 cells than in control cells (Fig 6C) Therefore, knockdown of the PRDX5 gene stimulated transcription of some families of human satellite DNA, ... menadione-induced cell death, making a small contribution to protection from oxidation of nuclear DNA induced by menadione Transcription of satellite DNA in the PRDX5 knockdown clone The human PRDX5 ... Journal compilation ª 2006 FEBS A Kropotov et al Transcription of noncoding DNA A B C E D Fig Analysis of 7,8-dihydro-8-oxoguanine (8-oxoG) in the PRDX5 knockdown clone KD-1 (A,B) and in A549 cells...

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Báo cáo khoa học: Catalytic transformations of supercoiled DNA as studied by ﬂow linear dichroism technique doc

Ngày tải lên : 30/03/2014, 11:20

... Math 45, 1–17 Frank-Kamenetskii MD (1993) Unraveling DNA VCH Publishers, New York Vologodskii AV, Anshelevich VV, Lukashin AV & Frank-Kamenetskii MD (1979) Statistical mechanics of supercoils and ... characters of catalysis by topoisomerase II qðtÞ ¼ DNA f½ð1 À k tÞqðsinit Þ þ k tÞqðrfin Þg; ð6Þ where k( t) is a degree of DNA conversion (2) When the enzyme changes the DLk of DNA strictly ... Comparison of the kinetics of inhibition of reaction of lg of pUC19 DNA with lg of topoisomerase II by etoposide and AMPPNP (A) lM (curve 1), lM (2), 10 lM (3), 25 lM (4), 50 lM (5) of etoposide;...

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