k na and ca concentrations in single cells

... 5391 Recombinant bovine zona pellucida glycoproteins S Kanai et al residues of the complex-type N-linked chains are involved in sperm binding [32] In cows, the major neutral N-linked chain of ZP ... zona pellucida 5 5K glycoproteins Mol Reprod Dev 30, 126–134 32 Yonezawa N, Amari S, Takahashi K, Ikeda K, Imai FL, Kanai S, Kikuchi K & Nakano M (2005) Participation of the nonreducing terminal ... Suzuki A, Takeda Y & Nakano M (2001) Essential role of the nonreducing terminal a-mannosyl residues of the N-linked carbohydrate chain of bovine zona pellucida glycoproteins in sperm–egg binding...

Ngày tải lên: 07/03/2014, 05:20

... sister kinetochores, and activates the spindle checkpoint protein BubR1 in the mitotically arrested HeLa cells We also present evidence indicating that the cells blocked at mitosis were eliminated ... resulting in the formation of the kinetochore fibre, leads to a reduction in the concentration of the motor proteins and spindle checkpoint proteins [19] This reduction of checkpoint proteins is ... lines of evidence indicate that the checkpoint proteins like Mad2, Bub1 and BubR1 can sense the attachment of microtubules and kinetochores, and the tension across the sister chromatids [17] Kinetochores...

Ngày tải lên: 30/03/2014, 10:20

... Immunohistochemistry Calbindin is expressed in the cerebellum exclusively by Purkinje cells [8,22] Anti-CaBP immunohistochemistry deposited peroxidase reaction product throughout all Purkinje cells, including ... on Purkinje cells and a persistent multiinnervation of Purkinje cells by olivary climbing fibers [7,26] The phase relationships to sinusoidal vestibular stimulation of floccular Purkinje cells ... phosphate-buffered saline (TPBS; 8.5 mM Na2 HPO47H2O, mM KH2PO4, 125 mM NaCl, 30 mM Tris-HCl, 0.03 mM NaN3, pH 7.7) Sections were incubated overnight at room temperature in rabbit polyclonal anti-calbindin-D (anti-CaBP,...

Ngày tải lên: 07/08/2014, 17:22

... AND METHODS growing conditions In March 1994, seeds of Pinus pinaster Ait., provenance Landes (southwest France), were individually germinated in L cylindrical containers filled with a peat and ... (figures and 2) gesting In contrast to our results obtained on needles, Nguyen and Lamant [9, 10] found osmotic adjustment of about 0.3 MPa, by a two-fold increase of pinitol in fine roots of P pinaster ... pinaster seedlings grown in mineral solution, as it was also mentioned by Popp and Smirnoff [ 14] in Cajanus cajan Can results obtained in such conditions extrapolate to more realistic drought induction...

Ngày tải lên: 08/08/2014, 14:21

... temperature in streptavidin–peroxidase complex (1:500 in DPBS; Dako), rinsed in distilled water and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase substrate (Sigma) for minutes and counterstained ... COL10A1, 5'TACCTTGTGCCTCCCATTCAA-3' (forward) and 5'-TACAGTACAGTGCATAAATAAATAATATATCTCCA-3' (reverse); COL11A2, 5'-CCTGAGCCACTGAGTATGTTCATT-3' (forward) and 5'-TTGCAGGATCAGGGAAAGTGA-3' (reverse); ... serum) with × insulin–transferrin– selenium supplement (ITS+1; final concentrations 10 μg/ml bovine insulin, 5.5 μg/ml transferrin, ng/ml sodium selenite, 4.7 μg/ml linoleic acid and 0.5 mg/ml...

Ngày tải lên: 09/08/2014, 10:20

... Efficient binding of NC2 TATA-binding protein to DNA in the absence of TATA J Biol Chem 2005, 280:6222-6230 31 Kawaji H, Kasukawa T, Fukuda S, Katayama S, Kai C, Kawai J, Carninci P, Hayashizaki Y: CAGE ... formation and TFIIB binding in vivo The high prevalence for the occurrence of motif combinations in TFIIB-dominated genes in illustrated in Figure 5e In line with the known synergy between INR and ... resolution Binding of TBP and the initiating form of RNAPII (phosphorylated at serine in its carboxy-terminal domain) were monitored in parallel All three factors showed pronounced binding to the...

Ngày tải lên: 09/08/2014, 20:21

... by the lack of information on the actual amounts of choline and phosphatidylcholine that were consumed by this patient during pregnancy and therefore whether inadequate dietary intake contributed ... factor in this case, as the patient maintained normal plasma concentrations of haemoglobin throughout the pregnancy Low weight gain during pregnancy is another risk factor that contributes to low infant ... expected increase controls showed the expected increase in plasma choline and phosphatidylcholine concentrations nutrients during pregnancy can prevent these effects [1,15] Conclusion Our data analysis...

Ngày tải lên: 11/08/2014, 10:23

... letters indicate exchanged amino acid residues Vertical lines indicate the beginning of the predicted transmembrane domains (B and C) Surface distribution of wild-type F and G proteins in polarized ... Prydz K, Kolsto AB, Prydz H: Opposite sorting of tissue factor in human umbilical vein endothelial cells and Madin-Darby canine kidney epithelial cells Blood 1996, 88:1339-1349 Roberts RL, Fine ... and G targeting in endothelial cells compared with epithelial cells Exchange of both tyrosine signals in the F protein led to an apical redistribution in endothelial cells whereas only tyrosine...

Ngày tải lên: 12/08/2014, 02:20

... 6.5, containing 150 mM NaCl, mM phenylmethylsulfonylfluoride and 75 units/ml aprotinin) and we determined its protein content (BCA methods) Next, we took a volume containing 4.5 mg of protein, a ... and in control and in minor amount in fraction 7, while in hypoxia almost doubled in fractions and Lipid analysis in DRF Cholesterol was enriched in DRF in control (878 ± 80, nmol/mg prot) and ... cellular integrity, considering that PLA2 activation in anoxia may lead to cellular death through a caspase independent mechanism by inducing nuclear shrinkage [22] In line, with these findings,...

Ngày tải lên: 12/08/2014, 16:20

... will be maintained as long as the inducing condition remains unchanged, the gene template continues to transit between the active and inactive state in individual cells k3 f k2 f, k 2f k2 b k3 b TA ... competing for the promoter binding sites, or by binding TA to block its DNA-binding or activational domains Interestingly, in two studies reporting that the same promoters were capable of producing ... (k2 f and k' 2f are the TA-dependent and TA-independent activation rates of the promoter, respectively; k5 = In 2/ t ( RNA ) , k7 = In 2/ t ( protein ) , where t ( RNA ) and t ( protein ) are RNA...

Ngày tải lên: 13/08/2014, 23:20

... Insulin regulates alternative splicing of protein kinase C beta II through a phosphatidylinositol 3-kinasedependent pathway involving the nuclear serine/argininerich splicing factor, SRp40, in ... of insulin and wingless signaling pathways in Drosophila S2 cells Figure Activation of insulin and wingless signaling pathways in Drosophila S2 cells (a) Schematic representation of the insulin ... upon activation of the insulin and wingless pathways in S2 cells Binding of insulin-like peptides to the insulin receptor in Drosophila cells leads to the activation of dPI3 kinase, which Genome...

Ngày tải lên: 14/08/2014, 21:20

... Radioanalytical and Nuclear Chemistry, doi: 10.1007/s10967-0066939-4 Sahin, L., Cetinkaya, H., Murat, Saç M., and Ichedef, M (2013) Determination of radon and radium concentrations in drinking ... 10.1016/j.apradiso.2007 Khattak, N U., Khan, M A., Shah, M T., and Javed, M W (2011) Radon concentrations in drinking water sources of the main campus of the University of Peshawar and surrounding areas, Khyber Pakhtunkhwa, ... Markandeya river basins, Karnataka State, India Journal of Radioanalytical and Nuclear Chemistry, doi: 10.1007/s10967-010-0573-x Institute for Global Environmental Strategies (IGES), 2007 Final...

Ngày tải lên: 08/09/2015, 20:16

... Guanosine triphosphate JNK c-Jun N-terminal kinase LIMK LIM domain kinase MCS Monte Carlo step MDCK Madin Darby canine kidney mDia Mammalian diaphanous (Diaphanous-related formin) MLC Myosin light ... of the cells, and show that cells in the front were moving faster than those further back [201, 192], especially in fingers of cells Interestingly, in Madin Darby canine kidney (MDCK) cells, the ... protein kinase RTK Receptor tyrosine kinase Src Sarcoma VASP Vasodilator-stimulated phosphoprotein WASP WiskottAldrich syndrome protein WAVE WASP-family verprolin-homologous protein WGAP WAVE-binding...

Ngày tải lên: 09/09/2015, 18:51

... pro-apoptotic activity of Bax and Bak by preventing the binding of these proteins to anti-apoptotic Bcl-2, suggesting that these pro-apoptotic proteins kill by binding and inhibiting their anti-apoptotic ... Nur77 (Fernandez-Sarabia and Bischoff 1993; Lin, Kolluri et al 2004) In the former interaction, although Ras is usually known to promote survival in tumor cells through the PI3-kinase/Akt pathway, ... oxidative inactivation of PTEN, redox regulation of MAPKs such as c-Jun N-terminal kinase, p38MAPK and ERK, as well as activation of transcription factors such as AP-1 and NF-κB (Heffetz, Bushkin et...

Ngày tải lên: 14/09/2015, 08:42

... been effective in detecting general adaptability of the [3H]ouabain binding site content and NKA isoforms following training, inactivity induced by injury and ageing (Leivseth & Reikeras, 1994; ... leading to increased intracellular accumulation of cAMP Monensin Increases Na+ concentration Insulin/IGF-1 Increases Na+ affinity of NKA Possible translocation role Veratridine Increases Na+ influx ... Adenosine diphosphate AP Action potential ATP Adenosine 5´ triphosphate BMI Body mass index CaMKII Ca2 + calmodulin-dependent protein kinase cAMP Adenosine 3´,5´-cyclic monophosphate CGRP Calcitonin...

Ngày tải lên: 04/12/2015, 13:59

... alterations in serum cation concentration, can be known as a risk factor for cataract formation Na+ pump activity in lens is as in other cells and it is related to intracellular Na+ , extracellular K+ and ... examined by an expert ophthalmologist and routine ophthalmic examinations including visual acuity determination, slit lamp examination, ophthalmoloscopy and intraocular pressure measurement were ... nutritional status in the process of cataract formation and the possibility that biochemical parameters can be used as markers to determine the risk involved for cataractogenesis, considering the...

Ngày tải lên: 03/11/2012, 09:49

... Solvation and energetic of binding of Na+ and K+ to pyruvate kinase It has been hypothesized [18] that the difference between Na+ and K+ in the activation and inhibition of pyruvate kinase could ... monovalent and divalent cation binding sites (Fig 2, inset) Competition between Na+ or K+ and Mg2+ + + Fig Effect of Na (A) and K (B) on the activity of pyruvate kinase in 100% water and in various ... solutions, and apparent binding energies for pyruvate kinase -K+ and pyruvate kinase -Na+ complexes in water and water/ dimethylsulfoxide mixtures Transfer energies (DG°t) were taken and transformed to kJÆmol)1...

Ngày tải lên: 08/03/2014, 02:20

... depolarizing and hyperpolarizing sides, because rectification was not incorporated into the basic units The myocardial cells were assumed to be cylindrical in shape, 150 µm in length and 16 µm in diameter ... ∆Vm in cell 51 was ca 84 mV and those in the contiguous cells were ca 44 mV (cells 50 & 52), ca 26 mV (cells 49 & 53), ca 15 mV (cells 48 & 54), and ca mV (cells 47 & 55) exponential fall-off in ... that in cells on either side (cells 50 & 52) was only about mV C: With 100 gj-channels, the ∆Vm in cell 51 was ca 120 mV, that in cells 50 and 52 was ca 40 mV, and that in cells 49 and 53 was ca...

Ngày tải lên: 13/08/2014, 16:21

... between ROS and mtDNA mutation rate was assessed using a linear correlation test Results CagA and VacA proteins As shown in Fig 1a and b, the CagA protein (120 kDa) and VacA protein (95 kDa) were ... with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli extract The Hp and E ... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain...

Ngày tải lên: 25/10/2012, 11:18

... for the regulation and localization of claudin expression It has been shown that protein kinases such as Protein Kinase A (PKA) and Protein Kinase C (PKC) can phosphorylate claudins (6,7) Phorbol ... Wnt signaling is involved in claudin-1 expression and localization 99 Indeed, a study shows that increasing β-catenin increases the expression of claudin-1 in colorectal cancer cells, and that ... Furuse M., Tsukita S., Niikawa N., Nakamura Y., and Furukawa Y Involvement of claudin-1 in the beta-catenin/Tcf 101 signaling pathway and its frequent upregulation in human colorectal cancers Oncol...

Ngày tải lên: 03/11/2012, 11:17